scholarly journals Evaluation of bactericidal effects of ultraviolet light C irradiation on cariogenic bacteria: An in vitro study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Moayyad Alharbi ◽  
Fahad Bakitian ◽  
Ali Alenezi
Keyword(s):  
Streptococcus Mutans ◽  
Ultraviolet Light ◽  
Single Layer ◽  
In Vitro Study ◽  
Bactericidal Effect ◽  
Oral Bacteria ◽  
Solid Surfaces ◽  
Limited Effect ◽  
Bactericidal Effects

Abstract Backgrounds Ultraviolet light C (UVL-C) irradiation has demonstrated an antimicrobial action against various pathogens. This study aimed to evaluate the bactericidal effect of UVL-C irradiation against cariogenic oral bacteria (Streptococcus mutans) in single layers and colonies grown on solid surfaces. Methods Two different experiments were performed. In the first experiment, a single layer of Streptococcus mutans bacteria on agar plates was exposed to UVL-C irradiation at energies from 0 to 21 mWs/cm2. The second experiment was conducted to inhibit viability of bacterial colonies on solid surfaces. The samples were derived from saliva from a patient where bacteria were grown on plastic strips and then exposed to UVL-C. The highest energy was 1050 mWs/cm2. Results Exposure to 21 mWs/cm2 was bactericidal in single layers of Streptococcus mutans. The result for bacterial colonies on solid surfaces indicated only a bacteriostatic effect, even at energies of 1050 mWs/cm2. Conclusions Ultraviolet light C exhibits bactericidal effects on single layers of Streptococcus mutans but has a limited effect on bacterial colonies in a biofilm. It is a matter of debate whether these in vitro results would have the same effect in clinical setting.

scholarly journals Anti-bacterial activity of three essential oils - An in vitro study

2019 ◽  
Vol 10 (2) ◽  
pp. 1049-1053 ◽  
Author(s):  
Geetha RV ◽  
John Rozar Raj B ◽  
Lakshmi Thangavelu
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Streptococcus Mutans ◽  
Bacterial Species ◽  
In Vitro Study ◽  
Oral Bacteria ◽  
Zone Of Inhibition ◽  
Thyme Oil ◽  
Rosemary Oil

To conduct a study regarding the antibacterial activity of essential oils against bacteria causing Caries. Essential oils are distillates of the volatile compounds of a plant’s secondary metabolism and may act as photoprotective agents. Their curative effect has been known since antiquity. It is based on a variety of pharmacological properties which are specific for each plant species. The mouth contains a variety of oral bacteria, but only a few species of bacteria are believed to cause dental caries. Antibacterial activity of the three essential oils, Rosemary oil, Holy basil oil, Thyme oil was screened against Streptococcus mutans, using disc diffusion technique. The rosemary oil was more effective against Streptococcus mutans with a zone of inhibition of 52 mm diameter (at concentration 200 µl), Rosemary oil showed a zone of inhibition of 44 mm diameter and with thyme oil, the zone diameter was 30 mm. The results of this study showed that the essential oils at different concentrations exhibited antibacterial activity against the bacterial species tested.

scholarly journals Activity of Human β-Defensin 3 Alone or Combined with Other Antimicrobial Agents against Oral Bacteria

2003 ◽  
Vol 47 (10) ◽  
pp. 3349-3351 ◽  
Author(s):  
Giuseppantonio Maisetta ◽  
Giovanna Batoni ◽  
Semih Esin ◽  
Filippo Luperini ◽  
Manuela Pardini ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Bactericidal Activity ◽  
Lactobacillus Acidophilus ◽  
Antimicrobial Agents ◽  
Bacterial Species ◽  
Bactericidal Effect ◽  
Oral Bacteria ◽  
Actinobacillus Actinomycetemcomitans ◽  
Streptococcus Sobrinus

ABSTRACT The in vitro activities of human β-defensin 3 (hBD-3) alone or combined with lysozyme, metronidazole, amoxicillin, and chlorhexidine were investigated with the oral bacteria Streptococcus mutans, Streptococcus sanguinis, Streptococcus sobrinus, Lactobacillus acidophilus, Actinobacillus actinomycetemcomitans, and Porphyromonas gingivalis. hBD-3 showed bactericidal activity against all of the bacterial species tested. The bactericidal effect was enhanced when the peptide was used in combination with the antimicrobial agents mentioned above.

Effects of Extracellular Plaque Components on the Chlorhexidine Sensitivity of Strains of Streptococcus mutans and Human Dental Plaque

1985 ◽  
Vol 64 (8) ◽  
pp. 1051-1054 ◽  
Author(s):  
L, E. Wolinsky ◽  
W.R. Hume
Keyword(s):  
Streptococcus Mutans ◽  
Mammalian Cells ◽  
Control Level ◽  
In Vitro Study ◽  
Fixed Time ◽  
Oral Bacteria ◽  
Thymidine Uptake ◽  
Cellular Division ◽  
In Vitro Susceptibility

An in vitro study was undertaken to determine the effects of sucrose-derived extracellular plaque components on the sensitivity of selected oral bacteria to chlorhexidine (CX). Cultures of Streptococcus mutans HS-6, OMZ-176, Ingbritt C, 6715-wtl3, and pooled human plaque were grown in trypticase soy media with or without 1 % sucrose. The sensitivity to CX of bacteria grown in each medium was determined by fixed-time exposure to CX and subsequent measurement of 3H-thymidine uptake. One-hour exposure to CX at concentrations of 10-4 M (0.01% w/v) or greater substantially inhibited subsequent cellular division among all the S. mutans strains and human plaque samples tested. An IC50 (the CX concentration which depressed 3H-thymidine incorporation to 50% of control level) of close to 10-4 M was noted for S. mutans strains HS- 6, OMZ-176, and 6715-wt13 when grown in the presence of sucrose. The same strains grown in cultures without added sucrose showed about a ten-fold greater sensitivity to CX (IC50 close to 10 -5 M). A three-fold difference was noted for S. mutans Ingbritt C. Only a slight increase in the IC50 was noted for the plaque samples cultured in sucrose-containing media, but their threshold for depression of 3H-thymidine uptake by CX was lower than that for the sucrose-free plaque samples. The study showed that extracellular products confer some protection against CX to the bacteria examined, and provided an explanation for the disparity between clinically-recommended concentrations for plaque suppression and data on in vitro susceptibility. Also, when compared with similarly-derived indices of susceptibility of mammalian cells to CX, the data obtained give rise to new possibilities for therapeutic use of CX or other bisguanides in dentistry.

scholarly journals The effect of Streptococcus mutans on the Corrosion Behavior of Nickel-Titanium Dental Alloys - In Vitro Study

2019 ◽  
Vol 8 (6) ◽  
pp. 435-440
Author(s):  
Asmaa Marda ◽  
Khadija Mouflih ◽  
Abdelkebir Bellaouchou ◽  
Abdallah Guenbour ◽  
Asmae Elmansari ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Electrochemical Impedance ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Oral Bacteria ◽  
Open Circuit ◽  
Nickel Titanium ◽  
Dental Alloys ◽  
Enrichment Medium

This study aimed to compare the resistance of dental alloys to corrosion in a solution containing oral bacteria named Streptococcus mutans (S.mutans). The electrochemical behavior of Nickel-Titanium (NiTi) was investigated in sterile Fusayama artificial saliva (AS) with the enrichment medium tryptic soy broth (TSB) in solution 1 and (AS) with (TSB) and bacteria in solution 2. The electrochemical procedures selected for this work were open circuit potentials (OCP), Potentiodynamic polarization (PDP), and electrochemical impedance spectroscopy (EIS). The NiTi surface was examined using optical microscopy.      After 24 hours of immersion in artificial saliva, the results have shown that NiTi revealed high corrosion reactivity in the presence of S. mutans and present pitting corrosion on the surface.

Exploring the anti-caries properties of baicalin against Streptococcus mutans: an in vitro study

Biofouling ◽  
2021 ◽  
pp. 1-9
Author(s):  
Arval Viji Elango ◽  
Sahana Vasudevan ◽  
Karthi Shanmugam ◽  
Adline Princy Solomon ◽  
Prasanna Neelakantan
Keyword(s):  
Streptococcus Mutans ◽  
In Vitro Study ◽  
Vitro Study

Bactericidal Effect of the Er:YAG Laser on Dental Implant Surfaces: An In Vitro Study

2002 ◽  
Vol 73 (11) ◽  
pp. 1292-1298 ◽  
Author(s):  
Matthias Kreisler ◽  
Wolfgang Kohnen ◽  
Claudio Marinello ◽  
Hermann Götz ◽  
Heinz Duschner ◽  
...  
Keyword(s):  
Dental Implant ◽  
Er:Yag Laser ◽  
In Vitro Study ◽  
Bactericidal Effect ◽  
Vitro Study

scholarly journals Effect of Pomegranate Juice Extract on Streptococcus mutans - An In Vitro Study

2021 ◽  
Vol 10 (4) ◽  
pp. 203-206
Author(s):  
Rohith A. Nair ◽  
Jitheesh Jain ◽  
Jaseela Praveena ◽  
Pooja M. R ◽  
Shishir Shetty ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
In Vitro Study ◽  
Vitro Study ◽  
Pomegranate Juice

scholarly journals Synergism of Streptococcus mutans and Candida albicans Reinforces Biofilm Maturation and Acidogenicity in Saliva: An In Vitro Study

2021 ◽  
Vol 10 ◽  
Author(s):  
Hye-Eun Kim ◽  
Yuan Liu ◽  
Atul Dhall ◽  
Marwa Bawazir ◽  
Hyun Koo ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Critical Role ◽  
In Vitro Study ◽  
Acidic Ph ◽  
Acidic Environment ◽  
Symbiotic Interaction ◽  
Biofilm Development

Early childhood caries, a virulent-form of dental caries, is painful, difficult, and costly to treat that has been associated with high levels of Streptococcus mutans (Sm) and Candida albicans (Ca) in plaque-biofilms on teeth. These microorganisms appear to develop a symbiotic cross-kingdom interaction that amplifies the virulence of plaque-biofilms. Although biofilm studies reveal synergistic bacterial-fungal association, how these organisms modulate cross-kingdom biofilm formation and enhance its virulence in the presence of saliva remain largely unknown. Here, we compared the properties of Sm and Sm-Ca biofilms cultured in saliva by examining the biofilm structural organization and capability to sustain an acidic pH environment conducive to enamel demineralization. Intriguingly, Sm-Ca biofilm is rapidly matured and maintained acidic pH-values (~4.3), while Sm biofilm development was retarded and failed to create an acidic environment when cultured in saliva. In turn, the human enamel slab surface was severely demineralized by Sm-Ca biofilms, while there was minimal damage to the enamel surface by Sm biofilm. Interestingly, Sm-Ca biofilms exhibited an acidic environment regardless of their hyphal formation ability. Our data reveal the critical role of symbiotic interaction between S. mutans and C. albicans in human saliva in the context of pathogenesis of dental caries, which may explain how the cross-kingdom interaction contributes to enhanced virulence of plaque-biofilm in the oral cavity.

Sign in / Sign up

Export Citation Format

Share Document