Formulation and Evaluation of Aloe Vera Gel Shampoo

Aloe Vera is a natural product which is now a days used in the field of cosmetology. Our formulation is to formulate a herbal shampoo using a plant extract which is used as hair washing and conditioning shampoo to treat hair loss and prevent itching scalp and dandruff. is effective than the marketed shampoo as we have used essential oils like coconut oil which is derived from the coconut palm fruit , lavender oil which is derived from the flowers of lavender, rosemary oil derived from the flowering tops of rosemary. eucalyptus oil which is derived from the leaves of eucalyptus plant and some other excepients like antioxidants stabilizers, solubilizing agents, surfactants, cleansing agents, coloring agents and preservatives. All of these are used for the aim to improve the smoothening , shining and growth of the hair. Our study has evaluated all the tests like foaming ability test, pH determination, skin irritancy test, conditioning performance, wettability and in all those test it has shown good results when compared to the marketed shampoo.

Effects of Rosemary Oil (Rosmarinus Officinalis) Supplementation on the Fate of the Transplanted Human Olfactory Bulb Neural Stem Cells Against Ibotenic Acid-Induced Neurotoxicity (Alzheimer Model) in Rat

Rosemary oil (ROO) is known to have multiple pharmacological effects: it is an antioxidant, an anti-inflammatory, and cytoprotective. In the present study, we examined the effects of ROO on Human olfactory bulb neuronal stem cells (hOBNSCs) after their transplantation into rats, with the ibotenic (IBO) acid-induced cognitive deficit model. After 7 weeks, cognitive functions were assessed using the Morris water maze (MWM). After two months blood and the hippocampus were collected for biochemical, gene expression, and histomorphometric analyses. Learning ability and memory function were significantly enhanced after hOBNSCs transplantation and were nearly returned to normal in the treated group. The IBO acid injection was associated with a significant decline of total leukocyte count (TLC) and a significant increase in total and toxic neutrophils. As well, the level of IL-1β, TNF-α CRP in serum and levels of MDA and NO in hippocampus tissue were significantly elevated, while antioxidant markers (CAT, GSH, and SOD) were reduced in treated tissue compared to controls. The administration of ROO before or with cell transplantation attenuated all these parameters. In particular, the level of NO nearly returned to normal when rosemary was administrated before cell transplantation. Gene expression analysis revealed the potential protective effect of ROO and hOBNSCs via down-expression of R-βAmyl and R- CAS 3 and R-GFAP genes.

A nanoemulsion of Rosmarinus officinalis L. essential oil with allelopathic effect against Lactuca sativa L. seeds / Uma nanoemulsão a partir do óleo essencial de Rosmarinus officinalis L com efeito alelopático em sementes de Lactuca sativa L

Plant's essential oils have a wide range of allelopathic effects with potential uses as bioherbicides. In addition, the application of oils through nanoemulsions represents a promising alternative for agriculture, as it offers better performance and lowers toxic waste generation. Therefore, this study aimed to evaluate the chemical constitution of Rosmarinus officinalis (rosemary) essential oil, as well as its effects on germination, initial growth, Catalase (CAT), Peroxidase (POX), and Superoxide-Dismutase (SOD) enzymes activity of Lactuca sativa seeds. Nanoemulsions were produced at 5% concentration and then diluted with distilled water to 5.0, 7.0, and 10.0 mg/mL. We compared data obtained using variance (ANOVA) analysis, followed by Tukey's test at 5% probability. Rosemary oil showed a wide variety of terpenoid compounds, mainly the 1,8-cineol monoterpene, which accounted for 46% of the sample. The oil showed a dose-dependent negative allelopathic influence on all variables analyzed, causing a drop in germination percentage (%G), germination speed index (GVI), mean germination time (MTG), and leaf and root length. There was no change in CAT and SOD activity. The POX activity showed a reduction starting at the concentration of 7.0 mg/mL. The results showed allelopathic effects of rosemary oil, with potential use as a natural bioherbicide.

Application of rosemary essential oil (Rosmarinus officinalis Linnaeus, 1753) for anesthesia and transport of bighead carp (Hypophthalmichthys nobilis Richardson, 1845) fingerlings

The aim of the present research is to study the efficacy of rosemary oil as an anesthetic for bighead carp (Hypophthalmichthys nobilis Richardson, 1845) and as an anesthetic used for transport of fingerlings. The fish used in the experiment have an average body weight (BW, g) of 42.13±17.90 and an average total length (TL, cm) of 16.54±2.32. Five treatments are conducted with five experimental concentrations: 0.20 ml.l-1, 0.30 ml.l-1, 0.40 ml.l-1, 0.50 ml.l-1 and 0.60 ml.l-1. For each concentration 10 fish are used or a total of 50 fish. For the transport experiment, 3 concentrations are used: 0.06 ml.l-1, 0.08 ml.l-1 and 0.10 ml.l-1. The applied stocking density is 3 fish/l. A total of 60 fish are used for each concentration or a total of 180 fish. The established results, for the first experiment, show that at concentration of 0.60 ml.l-1 the induction of anesthesia is the fastest (3.46 min). From all tested concentrations the recovery time is the shortest at the lowest concentration – 2.16 min (p≤0.001). The concentration of 0.10 ml.l-1 of rosemary oil can be used for transport of bighead carp fingerlings for a period from 1 to 2 hours. The concentration of 0.08 ml.l-1 can be applied when the duration of the transport is from 1 to 3 hours.

RESPONSE OF BROILER CHICKEN TO INOVO ADMINISTRATION OF DIFFERENT LEVELS OF ROSEMARY OIL(ROSMARINUS OFFICINALIS)

This study was conducted to determine the percentage of hatchability, post-hatch growth performance, immune response of broiler subjected to in- ovo injection of different doses of rosemary oil. A total of 300 eggs of ross broiler strain were obtained.The experiment distributed into five treatment groups: T1, Negative Control T2, positive control (in ovo injection of 0.05 ml distilled water) T3, in ovo injection of 0.05ml of rosemary oil; T4, in ovo injection of 0.075 ml of rosemary oil; T5, in ovo injection of 0.1 ml of rosemary oil.Highest hatchability percentage was shown in 0.1ml rosemary oil -injected hatching eggs. The final weight of birds from 0.075 and 0.1 ml of rosemary oil-injected eggs was significantly (P < 0.05) highest through the experiment. Feed intake and feed conversion ratio were improved with in ovo administration of rosemary oil at 0.075 and 0.1 ml doses. In addition, the level of blood cholesterol and blood glucose were decreased (p<0.05) in birds from rosemary oil-injected eggs. The obtained data showed a positive effect of in-ovo injection of rosemary oil on stimulating bird's immunity.

Comparing the Effects of Progressive Muscle Relaxation Technique and Aromatherapy With Rosemary Oil on Preoperative Anxiety in General Surgery Candidates

Objective: Anxiety is one of the most common problems of patients before surgery. This study aims to compare the effects of Progressive Muscle Relaxation (PMR) technique and aromatherapy with rosemary oil on preoperative anxiety in candidates for general surgery.The aim of this study was to determine the effect of progressive muscle relaxation technique and aromatherapy with rosemary on preoperative anxiety in candidates for general surgery. This study aimed to determine the effect of progressive muscle relaxation techniques and aromatherapy with rose on preoperative anxiety in patients undergoing general surgery was performed. Anxiety is one of the most common problems of patients before surgery. Anxiety is one of the most common disorders in patients prior to surgery Methods: In this clinical trial, participants were 90 patients who were candidates for general surgery referred to Ayatollah Kashani Hospital in Shahrekord, Iran in 2018. After obtaining informed written consent from them, they were randomly divided into three groups: PMR (n=30), aromatherapy (n=30) and control (n=30). The intervention was performed one hour before the surgery. Data collection tools were a demographic form and the Depression, Anxiety and Stress Scale - 21 Items. Collected data were analyzed using chi-square test, paired t-test, and one-way analysis of variance in SPSS v.21 software.In both groups, the intervention was performed by the patient one hour before the operation. In both groups an hour before the operation was performed by the patient. Data collection tools included demographic characteristics questionnaire, 21-item DASS anxiety scale. Data collection tools included demographic questionnaires anxiety scale survey had 21 questions DASS. In this clinical trial, 90 patients who were candidates for general surgery referred to Ayatollah Kashani Hospital in Shahrekord in 1398 were randomly divided into three groups: relaxation (30), aromatherapy (30) and control (30). In this clinical trial, 90 patients undergoing general surgery hospital Ayatollah Kashani In 1398 randomly into three groups: relaxation (30), aromatherapy (30) and control (30) were divided. In both groups, relaxation and aromatherapy techniques were performed by the patient one hour before the operation. In both groups, relaxation techniques and aromatherapy one hour before surgery was performed by the patient. Data were analyzed using SPSS software version 21. Data using SPSS version 21 was analyzed. Data collection tools included demographic characteristics questionnaire, 21-item DASS Anxiety Scale (DASS 21). Data collection tools included demographic questionnaires anxiety scale survey had 21 questions DASS. In both groups, relaxation and aromatherapy techniques were performed by the patient one hour before the operation. In both groups, relaxation techniques and aromatherapy one hour before surgery was performed by the patient. Results: Mean pre-intervention anxiety score was 16.97±2.77 and 16.80±2.66 in the PMR and aromatherapy groups, respectively (P˃0.05), which decreased to 16.03±2.54 and 15.03±2.88 after the intervention (P<0.05). There was no statistically significant difference between the two groups in terms of anxiety and the two methods were not superior to each other. But after the intervention, this difference became significant (P<0.05). But after this difference was significant (P<0.05). The results showed that the mean scores of anxiety before the intervention between the three groups were not statistically significant (P>0.05). Results showed that the mean score of anxiety before the intervention showed no significant difference between the three groups (P<0.05). Also, there was no statistically significant difference between the two groups in terms of the studied variable and the two methods of progressive muscle relaxation and aromatherapy were not superior to each other. Also compared between the two groups there was no significant difference variable and progressive muscle relaxation and aromatherapy superior to each other did not. Conclusion: PMR and aromatherapy with rosemary oil can be used in clinics to reduce the level of anxiety in patients before general surgery. According to the results of the study, it can be suggested that relaxation and aromatherapy be used to reduce the level of anxiety of patients before general surgery in the clinic. According to the results, it can be suggested that the relaxation and aromatherapy to reduce the level of public anxiety before surgery was clinical.

Fabrication of rosemary essential oil microcapsules and using in active packaging

Essential oils are interesting plant extracts that are used for preservation in food field. They can be used in antibacterial food packaging systems. The disadvantage of essential oils is that they degrade due to light and heat and lose their biological activity. Encapsulation techniques enable essential oils to be protected them. In this study wall material maltodextrin and whey protein concentrate were used. The emulsions were prepared with rosemary oil and wall material. Freeze dried capsules were synthesized. The encapsulation efficiencies, surface morphologies and particle size distributions were determined. In addition, the paper coating formulations were prepared with rosemary oil capsule by different concentrations and applicate them onto the paper. Antimicrobial test was applied to both gram negative and positive bacteria on the coated papers. In addition, color, gloss and contact angle were measured by spectrophotometer, glossmeter and goniometer, respectively. The offset test prints were made on the coated papers, and the color and gloss of the prints were measured and the color changes were calculated. As a result, capsules with rosemary oil were produced. It was determined that rosemary coated papers showed activity against E. coli bacteria and this paper could be used in active food packaging.

Rosemary oil nano-emulsion potentiates the apoptotic effect of mitomycin C on cancer cells in vitro

Purpose: To formulate nano-emulsified rosemary oil (REO/NE) and determine its effect on the anticancer agent, mitomycin C (MC) when used as a carrier for the drug. Methods: The droplet size of REO/NE was markedly enlarged when mixed with MC. The cytotoxicity of the formulations on HeLa and MCF-7 cells was determined using MTT assay. The combination index (CI) values were estimated with CompuSyn software, while apoptosis was determined using DAPI fluorescent dye. Results: Treatment of MCF-7 cells and HeLa cells with REO/NE (1% v:v and 1.33% v:v, respectively) reduced the IC50 of MC 33 and 15 folds, respectively. Under fluorescent microscopy, cells treated with REO/NE+MC had more marked reduction of the nuclear area than MC-treated cells. Conclusion: These results indicate that REO/NE is an efficient carrier for MC since it enhanced MC delivery and increased its effect on the cells through the induction of apoptosis at low concentrations of MC.