Effect of crude leaf extract of Osyris quadripartita on Plasmodium berghei in Swiss albino mice

A research was conducted to investigate the haematological effects of ethanolic leaf extracts of Senna occidentalis on Swiss albino mice infected with 0.2 ml of Plasmodium berghei infected blood. Fifteen (15) mice weighing between 140-260g were assigned into five study groups of three mice each. The first group is treated with 0.2 mL of normal saline (drug free control). Group 2, 3, 4 were treated with 100, 200, and 400mg/kg of theethanolic leaf extract respectively while group 5 received 10mg/kg of chloroquine phosphate. All doses were administered orally. The results obtained were analyzed using Analysis of Variance with Duncan’s Multiple Range Test to separate the means. The result of the preliminary phytochemical analysis revealed the presence of alkaloids, saponins, cardiac glycosides, diterpenoids, flavonoids, steroids, tannins, Triterpenoids, carbohydrates and proteins. The level of parasite suppression ranges from 35% to 75% and the activity increased with increase in concentration of the extracts (dose dependent). The extracts were found to increase the level of some haematological parameters such as Red Blood Cells, White Blood Cells and Haemoglobin. The effect is concentration dependent, increases with increase in concentration. Thus, the anti plasmodial efficacy of the leaf extract of S. occidentalis on P. berghei is confirmed. It is recommended that , 400 mg/kg leaf ethanolic extracts of S. occidentalis couldbe use in the treatment of malarial fever.

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In vivo Antiplasmodial Activity of Ethanolic Leaf Extract of Tithonia diversifolia (Hemsl.) A. Gray against Plasmodium berghei Nk65 in Infected Swiss Albino Mice

Journal of Applied Life Sciences International ◽

10.9734/jalsi/2016/28803 ◽

2016 ◽

Vol 8 (3) ◽

pp. 1-8

Author(s):

E Dada ◽

D Oloruntola

Keyword(s):

Plasmodium Berghei ◽

Leaf Extract ◽

Antiplasmodial Activity ◽

Tithonia Diversifolia ◽

Swiss Albino Mice ◽

Albino Mice ◽

Ethanolic Leaf Extract

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Phytochemical Analysis and Antiplasmodial (curative) Activities of Methanolic Leaf Extract of Morinda lucida (Ewe Oruwo) in Male Swiss Mice Infected with Plasmodium berghei NK65

International Journal of TROPICAL DISEASE & Health ◽

10.9734/ijtdh/2019/v37i130156 ◽

2019 ◽

pp. 1-13

Author(s):

Momoh Johnson Oshiobugie ◽

Damazio Olanrewaju Anthony ◽

Ajetunmobi Asibiallau Oladipupo ◽

Babalola Adenike Omosalewa ◽

Adekunle Oluwasegun Michael ◽

...

Keyword(s):

Body Weight ◽

Vitamin A ◽

Plasmodium Berghei ◽

Leaf Extract ◽

Liver Homogenate ◽

Haematological Parameters ◽

Swiss Albino Mice ◽

Swiss Mice ◽

Albino Mice ◽

Group B

Aim: Medicinal plants have been used for the treatment of many infections and diseases including malaria. The study was conducted to determine the effect of in vivo anti-plasmodial and antioxidant properties of the methanolic leaf extract of Morinda lucida in male Swiss albino mice infected with Plasmodium Berghei NK65. Study Design and Methodology: Phytochemical, GC-MS and AAS analyses were determined in the plant. Swiss albino mice were inoculated intraperitoneally with Plasmodium berghei NK65. Thirty-five (35) mice were grouped into seven groups, five per group. Group A were not infected with P.berghei NK65. Group B, C and D served as the negative and positive control groups while Group E, F and G mice were treated with 400, 600 and 800 mg/kg body weight of methanolic leaf extract of M. lucida. Haematological parameters were determined in the whole blood using BC-3200 Auto Hematology Analyzer. TP, MDA, CAT, SOD % inhibition, SOD unit and vitamin A were all determined in the liver homogenate using standard procedures. Results: The GC-MS result of the M. lucida shows the presence of five bioactive compounds. It was also observed that the plant contains the following minerals: iron, magnesium, potassium, phosphorus and copper. Acute toxicity shows that the LD50 >000mg/Kg b.wt. The extract caused 30.96%, 32.93% and 67.23% reduction in parasitemia at 400, 600 and 800 mg/kg body weight respectively while chloroquine exerted 96.53% and artesunate exerted 92.03% reduction at 10 mg/kg body weight respectively. The Haematological parameters showed that the plant extract is not haematotoxic since it significantly (P<0.05) reduced WBC count, and increase RBC, HGB, and HCT values in the treated mice compared to the infected untreated mice. This study shows that the mean lipid peroxidation (MDA) level was significantly decreased in the malaria treated mice (group C, D, E, F and G) compared to the untreated mice (group B). There was also a significant increase in the total protein, catalase, SOD % inhibition, SOD unit and Vitamin A levels in the liver homogenate of animals treated with chloroquine, artesunate and extract of M. lucida compared to the untreated mice. Conclusions: The study shows that Morinda lucida possess antiplasmodial activity in male Swiss mice infected with Plasmodium berghei NK 65.

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Tetrorchidium didymostemon leaf extract reduces Plasmodium berghei induced oxidative stress and hepatic injury in Swiss albino mice

Advances in Traditional Medicine ◽

10.1007/s13596-021-00548-9 ◽

2021 ◽

Author(s):

Osamudiamen Ebohon ◽

Francis Irabor ◽

Merit Esewi Ayevbuomwan ◽

Ehimwenma Sheena Omoregie

Keyword(s):

Oxidative Stress ◽

Plasmodium Berghei ◽

Leaf Extract ◽

Hepatic Injury ◽

Swiss Albino Mice ◽

Albino Mice

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Modulatory influence of Adhatoda vasica Nees leaf extract against gamma irradiation in Swiss albino mice

Phytomedicine ◽

10.1016/j.phymed.2003.12.006 ◽

2005 ◽

Vol 12 (4) ◽

pp. 285-293 ◽

Cited By ~ 19

Author(s):

A. Kumar ◽

J. Ram ◽

R.M. Samarth ◽

M. Kumar

Keyword(s):

Gamma Irradiation ◽

Leaf Extract ◽

Adhatoda Vasica ◽

Swiss Albino Mice ◽

Albino Mice

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Vernonia Amygdalina Del (Bitter Leaf) extract ameliorates isoniazid (INH) induced liver injury in Swiss Albino Mice

10.1101/482091 ◽

2018 ◽

Author(s):

Gebreselassie Addisu Tilaye ◽

Muluken Fekadie Zerihun ◽

Kasaw Adane Chuffa ◽

Mahelet Arayaselassie ◽

Daniel Seifu

Keyword(s):

Liver Injury ◽

Leaf Extract ◽

Drug Clearance ◽

Vernonia Amygdalina ◽

Drug Induced ◽

Standard Drug ◽

Swiss Albino Mice ◽

Antitubercular Drugs ◽

Toxic Injury ◽

Albino Mice

AbstractLiver plays a central role in the metabolism of drugs. Drug clearance and transformation exposes liver to toxic injury. Antitubercular drugs have been found to be hepatotoxic and potentially lead to drug-induced liver injury. Isoniazid is one of the most hepatotoxic first line antitubercular drugs. Conventional drugs used in the treatment of liver disease are often inadequate and a search for supplementation or alternative drugs for the treatment of hepatic damage is indispensible. Therefore our study aims to investigate the hepatoprotective potential of Vernonia Amygdalina Del (bitter leaf) extract against Isoniazid-induced liver injury in Swiss Albino Mice. Treatment of Mice orally with Vernonia Amygdalina Del extract at dose of 250mg/kg and 375 mg/kg significantly lowered (P<0.05) the serum level of liver enzymes in Isoniazid pretreated mice. The hepatoptotective activity of the extract found to be comparable with the standard drug, Silymarin (100 mg/kg, P.o.). Moreover, treatment with the extract significantly alleviated Isoniazid induced hepatic injury as supported by the photomicrographs of liver section of mice. The data shows aqueous Vernonia Amygdalina Del extract has a very promising hepatoprotective potential against isoniazid-induced liver injury.

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