Effects of Hydro-ethanolic Leaf Extract of Tithonia diversifolia on Parasitaemia Level, Serum Metabolites and Histopathology of Organs in Swiss Albino Mice Infected with Plasmodium berghei NK65

2017 ◽  
Vol 6 (2) ◽  
pp. 1-11 ◽  
Author(s):  
D Oloruntola ◽  
E Dada ◽  
I Osho ◽  
O Ogundolie
Keyword(s):  
Plasmodium Berghei ◽  
Leaf Extract ◽  
Tithonia Diversifolia ◽  
Serum Metabolites ◽  
Swiss Albino Mice ◽  
Parasitaemia Level ◽  
Albino Mice ◽  
Ethanolic Leaf Extract

scholarly journals Antiplasmodial Activity of Ethanolic Leaf Extract of Cymbopogon citratus (DC) Stapf in Swiss Albino Mice Infected with Plasmodium berghei NK 65

2021 ◽  
pp. 27-38
Author(s):  
E. O. Dada ◽  
R. O. Adebayo
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Plasmodium Berghei ◽  
Leaf Extract ◽  
Negative Control ◽  
Antiplasmodial Activity ◽  
Cymbopogon Citratus ◽  
Parasitaemia Level ◽  
Albino Mice ◽  
Ethanolic Leaf Extract

The study assessed the antiplasmodial activity of the ethanolic leaf extract of Cymbopogon citratus on chloroquine sensitive Plasmodium berghei in mice. Standard methods were used to determine the bioactive components of the leaf extract, acute toxicity test and antiplasmodial activity.  Mice obtained (of body weight 20-25 g) were housed and acclimatized for seven days at room temperature before the commencement of the experiment. A total of 16 albino mice were randomized into four groups of four mice each for acute toxicity while 35 were grouped into five groups of seven mice each for antiplasmodial activity. All the groups 1-5 were infected with P. berghei and were treated for six consecutive days with leaf extract dosage of 200, 400 and        800 mg/kg, standard antimalarial drug (chloroquine) as positive control and normal saline as negative control respectively. Phytochemical screening/ bioactive compounds of the leaf extract reveals the presence of saponins (10.3 mg/g), tannins (2.38 mg/g), flavonoids (1.87 mg/g), terpenoids (19.12 mg/g), steroids (6.21 mg/g) and glycosides (19.9 mg/g) as secondary metabolites. The leaf extract revealed decrease in body weight of the infected mice and did not show any toxicity at all dosage levels used. The antiplasmodial investigation revealed a decrease in percentage parasitaemia level in mice of extract treated groups compared with mice infected and not treated. The parasitaemia reduction was higher in 800 mg/kg than 200 mg/kg and 400 mg/kg. This significant decrease (P<0.05) in percentage parasitaemia level in the study was dose and time-dependent. The extract showed significant (p<0.05) antiplasmodial activity and could serve as possible candidates for the development of new effective drugs for the treatment of malaria.

scholarly journals Antiplasmodial and haematological effects of Senna occidentalis leaf ethanolic extracts on Swiss albino mice infected with Plasmodium berghei

2021 ◽  
Vol 12 (2) ◽  
pp. 122-128
Author(s):  
S.M. Babangida ◽  
G.J. Sow ◽  
D.M. Shehu
Keyword(s):  
Plasmodium Berghei ◽  
Blood Cells ◽  
Leaf Extract ◽  
White Blood Cells ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Swiss Albino Mice ◽  
Ethanolic Extracts ◽  
Albino Mice

A research was conducted to investigate the haematological effects of ethanolic leaf extracts of Senna occidentalis on Swiss albino mice infected with 0.2 ml of Plasmodium berghei infected blood. Fifteen (15) mice weighing between 140-260g were assigned into five study groups of three mice each. The first group is treated with 0.2 mL of normal saline (drug free control). Group 2, 3, 4 were treated with 100, 200, and 400mg/kg of theethanolic leaf extract respectively while group 5 received 10mg/kg of chloroquine phosphate. All doses were administered orally. The results obtained were analyzed using Analysis of Variance with Duncan’s Multiple Range Test to separate the means. The result of the preliminary phytochemical analysis revealed the presence of alkaloids, saponins, cardiac glycosides, diterpenoids, flavonoids, steroids, tannins, Triterpenoids, carbohydrates and proteins. The level of parasite suppression ranges from 35% to 75% and the activity increased with increase in concentration of the extracts (dose dependent). The extracts were found to increase the level of some haematological parameters such as Red Blood Cells, White Blood Cells and Haemoglobin. The effect is concentration dependent, increases with increase in concentration. Thus, the anti plasmodial efficacy of the leaf extract of S. occidentalis on P. berghei is confirmed. It is recommended that , 400 mg/kg leaf ethanolic extracts of S. occidentalis couldbe use in the treatment of malarial fever.

scholarly journals Phytochemical Analysis and Antiplasmodial (curative) Activities of Methanolic Leaf Extract of Morinda lucida (Ewe Oruwo) in Male Swiss Mice Infected with Plasmodium berghei NK65

2019 ◽  
pp. 1-13
Author(s):  
Momoh Johnson Oshiobugie ◽  
Damazio Olanrewaju Anthony ◽  
Ajetunmobi Asibiallau Oladipupo ◽  
Babalola Adenike Omosalewa ◽  
Adekunle Oluwasegun Michael ◽  
...  
Keyword(s):  
Body Weight ◽  
Vitamin A ◽  
Plasmodium Berghei ◽  
Leaf Extract ◽  
Liver Homogenate ◽  
Haematological Parameters ◽  
Swiss Albino Mice ◽  
Swiss Mice ◽  
Albino Mice ◽  
Group B

Aim: Medicinal plants have been used for the treatment of many infections and diseases including malaria. The study was conducted to determine the effect of in vivo anti-plasmodial and antioxidant properties of the methanolic leaf extract of Morinda lucida in male Swiss albino mice infected with Plasmodium Berghei NK65. Study Design and Methodology: Phytochemical, GC-MS and AAS analyses were determined in the plant. Swiss albino mice were inoculated intraperitoneally with Plasmodium berghei NK65. Thirty-five (35) mice were grouped into seven groups, five per group. Group A were not infected with P.berghei NK65. Group B, C and D served as the negative and positive control groups while Group E, F and G mice were treated with 400, 600 and 800 mg/kg body weight of methanolic leaf extract of M. lucida. Haematological parameters were determined in the whole blood using BC-3200 Auto Hematology Analyzer. TP, MDA, CAT, SOD % inhibition, SOD unit and vitamin A were all determined in the liver homogenate using standard procedures. Results: The GC-MS result of the M. lucida shows the presence of five bioactive compounds. It was also observed that the plant contains the following minerals: iron, magnesium, potassium, phosphorus and copper. Acute toxicity shows that the LD50 >000mg/Kg b.wt. The extract caused 30.96%, 32.93% and 67.23% reduction in parasitemia at 400, 600 and 800 mg/kg body weight respectively while chloroquine exerted 96.53% and artesunate exerted 92.03% reduction at 10 mg/kg body weight respectively. The Haematological parameters showed that the plant extract is not haematotoxic since it significantly (P<0.05) reduced WBC count, and increase RBC, HGB, and HCT values in the treated mice compared to the infected untreated mice. This study shows that the mean lipid peroxidation (MDA) level was significantly decreased in the malaria treated mice (group C, D, E, F and G) compared to the untreated mice (group B). There was also a significant increase in the total protein, catalase, SOD % inhibition, SOD unit and Vitamin A levels in the liver homogenate of animals treated with chloroquine, artesunate and extract of M. lucida compared to the untreated mice. Conclusions: The study shows that Morinda lucida possess antiplasmodial activity in male Swiss mice infected with Plasmodium berghei NK 65.

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