Larrea divaricata Cav. aqueous extract and nordihydroguariaretic acid modulate oxidative stress in submandibular glands of diabetic rats: a buccal protective in diabetes

Diabetes mellitus has been reported to affect functions of the hippocampus. We hypothesized thatCentella asiatica, a herb traditionally being used to improve memory, prevents diabetes-related hippocampal dysfunction. Therefore, the aim of this study was to investigate the protective role ofC. asiaticaon the hippocampus in diabetes.Methods. Streptozotocin- (STZ-) induced adult male diabetic rats received 100 and 200 mg/kg/day body weight (b.w)C. asiaticaleaf aqueous extract for four consecutive weeks. Following sacrifice, hippocampus was removed and hippocampal tissue homogenates were analyzed for Na+/K+-, Ca2+- and Mg2+-ATPases activity levels. Levels of the markers of inflammation (tumor necrosis factor, TNF-α; interleukin, IL-6; and interleukin, IL-1β) and oxidative stress (lipid peroxidation product: LPO, superoxide dismutase: SOD, catalase: CAT, and glutathione peroxidase: GPx) were determined. The hippocampal sections were visualized for histopathological changes.Results. Administration ofC. asiaticaleaf aqueous extract to diabetic rats maintained near normal ATPases activity levels and prevents the increase in the levels of inflammatory and oxidative stress markers in the hippocampus. Lesser signs of histopathological changes were observed in the hippocampus ofC. asiaticaleaf aqueous extract treated diabetic rats.Conclusions.C. asiaticaleaf protects the hippocampus against diabetes-induced dysfunction which could help to preserve memory in this condition.

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Positive moderation of the hematology, plasma biochemistry and ocular indices of oxidative stress in alloxan-induced diabetic rats, by an aqueous extract of the leaves of Sansevieria liberica Gerome and Labroy

Asian Pacific Journal of Tropical Medicine ◽

10.1016/s1995-7645(12)60196-5 ◽

2013 ◽

Vol 6 (1) ◽

pp. 27-36 ◽

Cited By ~ 6

Author(s):

Ikewuchi Jude Chigozie ◽

Ikewuchi Catherine Chidinma

Keyword(s):

Oxidative Stress ◽

Aqueous Extract ◽

Diabetic Rats ◽

Plasma Biochemistry

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N-acetylcysteine supplementation remodels thiol-related biochemical pathways towards decreased oxidation in diabetic submandibular glands

Research Society and Development ◽

10.33448/rsd-v10i3.13169 ◽

2021 ◽

Vol 10 (3) ◽

pp. e14510313169

Author(s):

Aluísio Eustáquio de Freitas Miranda Filho ◽

Ana Carolina Guimarães Ribeiro ◽

Nadine Francine Marcula Linhares Nunes ◽

Samuel Nuno Pereira Lima ◽

Vinicio Felipe Brasil Rocha ◽

...

Keyword(s):

Oxidative Stress ◽

Therapeutic Process ◽

Relative Weight ◽

Reducing Power ◽

Salivary Secretion ◽

Diabetic Rats ◽

Submandibular Glands ◽

Biochemical Alterations ◽

Stress Markers ◽

The Status

Secondary disorders in consequences to diabetes involves the development of several diseases in the oral cavity, as periodontitis, xerostomy, infection by diverse pathogens and dysfunctions on the salivary secretion. These alterations occur partially, in consequence of the oxidative stress occasioned by hyperglycemia, and are important in patients undiagnosed or that have flaws in their therapeutic process. The aim of this work was to evaluate biochemical alterations of submandibular glands in response to oxidative stress during diabetes mellitus, and verify the effects of N-acetylcystein supplementation to diabetic rats, specially on the regulation of modifications related to glutathione and thiol proteins. For this purpose, the levels of some oxidative stress markers and the occurrence of the post-translational event of S-glutathionylation were evaluated. The a-amilase degranulation by isolated acinar cells and glandular relative weight was also measured for each experimental group. The compound was able to decrease the lipoperoxidation and proteic oxidation observed in the submandibular gland of diabetic rats, preventing the decrease of the tecidual reducing power and increasing the occurrence of the post-translational process of S-glutathionylation. The diabetic condition increases the degranulation of a-amilase and the glandular weight, but the supplementation with N-acetylcystein did not affect these events. Together these findings may help to elucidate the status of oxidative stress on salivary glands and suggest new therapeutic strategies employing antioxidants of low molecular weight to prevent oral and systemic dysfunctions related to diabetes.

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