GC-MS profiling and assessment of antioxidant, antibacterial, and anticancer properties of extracts of Annona squamosa L. leaves

Abstract Background The research and application of plants in food supplements and drugs have attracted great interest. This study aimed to examine the efficiency of several solvents for the extraction of the main compounds from Annona squamosa leaves and to evaluate the antioxidant, antibacterial, and anticancer activities of these extracts. Methods Gas chromatography-mass spectrometry was used to screen the bioactive compounds of A. squamosa methanolic extract. The free radical, hydrogen peroxide, and nitric oxide scavenging activities of the extracts were investigated. Furthermore, MTT, nuclear staining, LDH, and monolayer wound repair assays were performed to evaluate the potential anticancer activity of the extracts in colon cancer cells while the antibacterial activity was tested by using a well diffusion assay. Results A. squamosa leaves extracts were found to contain several bioactive compounds, of which the majority were sesquiterpenes (C15H24). These extracts exhibited strong antioxidant activity and antibacterial potency against both gram-positive and gram-negative bacteria. Different A. squamosa leaves extracts displayed remarkable antiproliferative, cytotoxic, antimigration, and apoptotic activities in colon cancer cells. Conclusions A. squamosa leaves contain major bioactive compounds that inhibit the growth of several types of bacteria and colon cancer cell lines, which demonstrated their efficacy as an alternative source of antibiotics and for the development of novel drugs for colon cancer therapy.

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Anticancer Activities of Biogenic Silver Nanoparticles Targeting Apoptosis and Inflammatory Pathways in Colon Cancer Cells

Journal of Cluster Science ◽

10.1007/s10876-021-02143-z ◽

2021 ◽

Author(s):

Vydyanath R. Narasimha ◽

T. Sree Latha ◽

Reddanna Pallu ◽

Kalpana Panati ◽

Venkata Ramireddy Narala

Keyword(s):

Colon Cancer ◽

Silver Nanoparticles ◽

Cancer Cells ◽

Colon Cancer Cells ◽

Anticancer Activities ◽

Biogenic Silver Nanoparticles ◽

Inflammatory Pathways

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Effects of Hexane Root Extract of Ferula hermonis Boiss. on Human Breast and Colon Cancer Cells: An In Vitro and In Vivo Study

BioMed Research International ◽

10.1155/2019/3079895 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12 ◽

Cited By ~ 1

Author(s):

Nael Abutaha ◽

Fahd A. Nasr ◽

Mohammed Al-zharani ◽

Ali S. Alqahtani ◽

Omar M. Noman ◽

...

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Root Extract ◽

Therapeutic Effects ◽

Nuclear Staining ◽

Colon Cancer Cells ◽

Baccatin Iii ◽

Colon Cancers

Breast and colon cancers are leading causes of cancer-related deaths globally. Plants are a potential source of natural products that may be used for the treatment of cancer. Ferula hermonis (FH) is reported to have diverse therapeutic effects. However, there are few reports on the in vitro anticancer potential of FH extract. Our results showed that the Ferula hermonis root hexane extract (FHRH) can induce dose-dependent cytotoxic effects in breast and colon cancer cells with MTT IC50 values of 18.2 and 25 μg/ml, respectively. The FHRH extract induced apoptosis in both breast and colon cancer cells; this was confirmed by light and nuclear staining, q-PCR, and caspase 3/7 activation. This study also demonstrated the antitumor activity of FHRH in 9,10-dimethylbenz[α]anthracene DMBA-induced rodent mammary tumor model. The GC/MS analysis revealed the presence of 3,5-Dimethylbenzenemethanol, Alpha-Bisabolol, Alpha-pinene, Beta-pinene, and Baccatin III that have various pharmacological potentials. Overall, the present study suggests that FHRH extract possesses anticancer potential which is mediated through apoptotic effects in MDA-MB-231 and LoVo cells. The present study also considered a basis for further investigations into the potential use of FHRH extract as an anticancer therapy for breast and colon cancers.

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Effect of Dietary Bioactive Compounds on Thioredoxin Reductase 1 and 15kDa Selenoprotein Expression in Colon Cancer Cells

The FASEB Journal ◽

10.1096/fasebj.29.1_supplement.759.8 ◽

2015 ◽

Vol 29 (S1) ◽

Author(s):

Lauren Rosso ◽

Sarah Galinn ◽

Bradley Carlson ◽

Ryuta Tobe ◽

Salvador Naranjo‐Suarez ◽

...

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Bioactive Compounds ◽

Thioredoxin Reductase ◽

Colon Cancer Cells ◽

Thioredoxin Reductase 1 ◽

Dietary Bioactive Compounds

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Anticancer Activity of Mixed Doxorubicin and Pravastatin in Nanoemulsions Against HCT 116 Colon Cancer Cells

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v7i1.8918 ◽

2017 ◽

Vol 7 (1) ◽

Author(s):

Mayson Alkhatib ◽

Retnowati . ◽

Amalia F.

Keyword(s):

Colon Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Therapeutic Potential ◽

Morphological Changes ◽

Weight Ratio ◽

Nuclear Staining ◽

Colon Cancer Cells ◽

Fixed Weight ◽

Hct 116

Combining drugs with different mechanism of action in nanocarriers is becoming a promising strategy in cancer therapy. In the present study, the anticancer activity of the combination of doxorubicin (DOX) and pravastatin (PRV) loaded in nanoemulsions (NEs) was evaluated in HCT 116 colon cancer cells. The NE formulas (NEa and NEb) consisted of different weight fractions of the surfactant mixture of Eumulgin HRE 40/ Soya phosphatidylcholine/ sodium oleate at a fixed weight ratio of 3.5:3.0:3.5, cholesterol (CHO), Tris- HCl buffer (pH 7.22), and 1-octanol. The cytotoxicity of the drug formulas, loaded in either water or NEs, was assessed through 3-(4, 5 Dimethylthiazole- 2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay, while the mechanism of cell death was determined by observing the morphological changes of treated cells under light microscope and identifying apoptosis by using the ApopNexin FITC kit and DAPI nuclear staining. It has been found that reducing the concentration of DOX from 15 to 7.5µM by formulating it with 7.5µM of PRV in NEa (NEa (1 DOX:1 PRV)) has preserved its cytotoxicity against HCT-116 cancer cells. The present study proved that the combination of the PRV and DOX loaded in NEa formulations improved the therapeutic potential of both of PRV and DOX as anticancer drugs.

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Ethyl Acetate Extracts of Two Artemisia Species: Analyses of Phenolic Profile and Anticancer Activities Against SW-480 Colon Cancer Cells

Natural Product Communications ◽

10.1177/1934578x19843011 ◽

2019 ◽

Vol 14 (5) ◽

pp. 1934578X1984301

Author(s):

Marina Radović Jakovljević ◽

Darko Grujičić ◽

Marko Živanović ◽

Milan Stanković ◽

Andrija Ćirić ◽

...

Keyword(s):

Colon Cancer ◽

Ethyl Acetate ◽

Cancer Cells ◽

Early Stage ◽

Human Colon ◽

Colon Cancer Cells ◽

Anticancer Activities ◽

Human Colon Cancer ◽

Phenolic Profile ◽

Ethyl Acetate Extracts

Because Artemisia vulgaris L. and Artemisia alba Turra are traditional medicinal plants used for the treatment of different diseases, we evaluated the cytotoxic/apoptotic activity of ethyl acetate extracts from these natural products against human colon cancer cells SW-480. The extracts contained a large amount of the total polyphenols and flavonoids. The phenolic profile showed the presence of phenolic acids (gallic, p-coumaric, vanillic, and ferulic acids) and flavonoids (rutin, myricetin, luteolin, quercetin, and apigenin). 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay indicated that antiproliferative activities of both A. vulgaris and A. alba extracts increased with the extension of time exposure, with decreasing IC50 values. Mitomycin C (MMC) alone had antiproliferative activity, but in combination with plant extracts caused stronger effect with lower IC50 values. Flow cytometry analyses showed that A. alba extract induced higher percentage of SW-480 cells in the early stage of apoptosis (33.5 ± 1.6 vs 0.7 ± 0.1, P < 0.05), whereas the A. vulgaris extract significantly increased the percentage of cells in necrosis (82.4 ± 5.0 vs 53.9 ± 2.3, P < 0.05). In conclusion, A. alba extract can be considered a potential source of bioactive components with anticancer activity or be used as a dietary food supplement or supplement to chemotherapy due to its synergistic effect with the MMC.

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