Anticancer Activity of Mixed Doxorubicin and Pravastatin in Nanoemulsions Against HCT 116 Colon Cancer Cells

Combining drugs with different mechanism of action in nanocarriers is becoming a promising strategy in cancer therapy. In the present study, the anticancer activity of the combination of doxorubicin (DOX) and pravastatin (PRV) loaded in nanoemulsions (NEs) was evaluated in HCT 116 colon cancer cells. The NE formulas (NEa and NEb) consisted of different weight fractions of the surfactant mixture of Eumulgin HRE 40/ Soya phosphatidylcholine/ sodium oleate at a fixed weight ratio of 3.5:3.0:3.5, cholesterol (CHO), Tris- HCl buffer (pH 7.22), and 1-octanol. The cytotoxicity of the drug formulas, loaded in either water or NEs, was assessed through 3-(4, 5 Dimethylthiazole- 2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay, while the mechanism of cell death was determined by observing the morphological changes of treated cells under light microscope and identifying apoptosis by using the ApopNexin FITC kit and DAPI nuclear staining. It has been found that reducing the concentration of DOX from 15 to 7.5µM by formulating it with 7.5µM of PRV in NEa (NEa (1 DOX:1 PRV)) has preserved its cytotoxicity against HCT-116 cancer cells. The present study proved that the combination of the PRV and DOX loaded in NEa formulations improved the therapeutic potential of both of PRV and DOX as anticancer drugs.

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Cytotoxic Effect of the Combination of Gemcitabine and Atorvastatin Loaded in Microemulsion on the HCT116 Colon Cancer Cells

International Journal of Pharmaceutical and Clinical Research ◽

10.25258/ijpcr.v9i2.8298 ◽

2017 ◽

Vol 9 (2) ◽

Cited By ~ 1

Author(s):

Mayson H. Alkhatib ◽

Dalal Al-Saedi ◽

Wadiah S. Backer

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Anticancer Drugs ◽

Therapeutic Potential ◽

Morphological Changes ◽

In Vitro Study ◽

Colon Cancer Cells ◽

Apoptosis Detection ◽

Hct116 Cells

The combination of anticancer drugs in nanoparticles has great potential as a promising strategy to maximize efficacies by eradicating resistant, reduce the dosage of the drug and minimize toxicities on the normal cells. Gemcitabine (GEM), a nucleoside analogue, and atorvastatin (ATV), a cholesterol lowering agent, have shown anticancer effect with some limitations. The objective of this in vitro study was to evaluate the antitumor activity of the combination therapy of GEM and ATVencapsulated in a microemulsion (ME) formulation in the HCT116 colon cancer cells. The cytotoxicity and efficacy of the formulation were assessed by the 3- (4,5dimethylthiazole-2-yl)-2,5-diphyneltetrazolium bromide (MTT) assay. The mechanism of cell death was examined by observing the morphological changes of treated cells under light microscope, identifying apoptosis by using the ApopNexin apoptosis detection kit, and viewing the morphological changes in the chromatin structure stained with 4′,6-diamidino-2-phenylindole (DAPI) under the inverted fluorescence microscope. It has been found that reducing the concentration of GEM loaded on ME (GEM-ME) from 5μM to 1.67μM by combining it with 3.33μM of ATV in a ME formulation (GEM/2ATV-ME) has preserved the strong cytotoxicity of GEM-ME against HCT116 cells. The current study proved that formulating GEM with ATV in ME has improved the therapeutic potential of GEM and ATV as anticancer drugs.

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Ultrasound-enhanced biosynthesis of uniform ZnO nanorice using Swietenia macrophylla seed extract and its in vitro anticancer activity

Nanotechnology Reviews ◽

10.1515/ntrev-2021-0044 ◽

2021 ◽

Vol 10 (1) ◽

pp. 572-585

Author(s):

Darren Yi Sern Low ◽

Camille Keisha Mahendra ◽

Janarthanan Supramaniam ◽

Loh Teng Hern Tan ◽

Learn Han Lee ◽

...

Keyword(s):

Colon Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Hexagonal Wurtzite Structure ◽

Colon Cancer Cells ◽

Swietenia Macrophylla ◽

Zno Nps ◽

Human Colon Cancer ◽

Hct 116

Abstract In this study, ultrasonically driven biosynthesis of zinc oxide nanoparticles (ZnO NPs) using Swietenia macrophylla seed ethyl acetate fraction (SMEAF) has been reported. X-ray powder diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) analyses confirmed the presence of a pure hexagonal wurtzite structure of ZnO. Field emission scanning electron microscope images revealed the formation of uniquely identifiable uniform rice-shaped biologically synthesized ZnOSMEAF particles. The particle sizes of the biosynthesized NPs ranged from 262 to 311 nm. The underlying mechanisms for the biosynthesis of ZnOSMEAF under ultrasound have been proposed based on FTIR and XRD results. The anticancer activity of the as-prepared ZnOSMEAF was investigated against HCT-116 human colon cancer cell lines via methyl thiazolyl tetrazolium assay. ZnOSMEAF exhibited significant anticancer activity against colon cancer cells with higher potency than ZnO particles prepared using the chemical method and SMEAF alone. Exposure of HCT-116 colon cancer cells to ZnOSMEAF promoted a remarkable reduction in cell viability in all the tested concentrations. This study suggests that green sonochemically induced ZnO NPs using medicinal plant extract could be a potential anticancer agent for biomedical applications.

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In Vitro Anticancer Activity of Au, Ag Nanoparticles Synthesized Using Commelina nudiflora L. Aqueous Extract Against HCT-116 Colon Cancer Cells

Biological Trace Element Research ◽

10.1007/s12011-016-0666-7 ◽

2016 ◽

Vol 173 (2) ◽

pp. 297-305 ◽

Cited By ~ 32

Author(s):

Palaniselvam Kuppusamy ◽

Solachuddin J. A. Ichwan ◽

Putri Nur Hidayah Al-Zikri ◽

Wastuti Hidayati Suriyah ◽

Ilavenil Soundharrajan ◽

...

Keyword(s):

Colon Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Aqueous Extract ◽

Ag Nanoparticles ◽

Colon Cancer Cells ◽

Hct 116

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Purpurin binding interacts with LHPP protein that inhibits PI3K/AKT phosphorylation and induces apoptosis in colon cancer cells HCT‐116

Journal of Biochemical and Molecular Toxicology ◽

10.1002/jbt.22665 ◽

2020 ◽

Author(s):

Zhiwen Li ◽

Xu Zhou ◽

Huaqiang Zhu ◽

Xie Song ◽

Hengjun Gao ◽

...

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Colon Cancer Cells ◽

Akt Phosphorylation ◽

Hct 116

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Methylsulfonylmethane Induces p53 Independent Apoptosis in HCT-116 Colon Cancer Cells

International Journal of Molecular Sciences ◽

10.3390/ijms17071123 ◽

2016 ◽

Vol 17 (7) ◽

pp. 1123 ◽

Cited By ~ 10

Author(s):

Arzu Karabay ◽

Asli Koc ◽

Tulin Ozkan ◽

Yalda Hekmatshoar ◽

Asuman Sunguroglu ◽

...

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Colon Cancer Cells ◽

Hct 116

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Limonene and BEZ 235 inhibits growth of COLO-320 and HCT-116 colon cancer cells

International Journal of Drug Delivery ◽

10.5138/09750215.1919 ◽

2016 ◽

Vol 8 (3) ◽

pp. 89 ◽

Cited By ~ 2

Author(s):

Raja Ratna Reddy Yakkanti ◽

P Chandra Sekhar ◽

K Bharath Nandhan Reddy ◽

S Ramamoorthy ◽

S Ranga Suresh ◽

...

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Colon Cancer Cells ◽

Wild Type ◽

Anticancer Effects ◽

Clonogenic Potential ◽

Hct 116 ◽

Cancer Types ◽

Hct 116 Cells ◽

Preclinical And Clinical Studies

<p>D-Limonene is a dietary monoterpene with significant anticancer activity against many cancer types in preclinical and clinical studies. The study is designed to investigate synergistic anticancer effects of limonene and BEZ235 combination in COLO-320 and HCT-116 colon cancer cells. Cells were treated with both the drugs alone and in combination and the effects on cell viability; cell migration and clonogenic potential were examined. Results show that both drugs exhibited dose and time dependant cytotoxicity on the cell lines tested. CompuSyn analysis of the drug combination effects revealed the strong synergistic interaction of the combination. Our results also indicate that COLO-320 cells were more sensitive for anticancer effects of the drugs than HCT-116 cells. The presence of Ras and PI3K mutations in HCT-116 cells could possibly be one of the main reasons for the observed outcome as compared to the wild type expressions of them in COLO-320 cells.</p>

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THE INHIBITION OF POLYISOPRENOIDS FROM NYPA FRUTICANS LEAVES ON CYCLOOXYGENASE 2 EXPRESSION OF WIDR COLON CANCER CELLS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i8.26098 ◽

2018 ◽

Vol 11 (8) ◽

pp. 154 ◽

Cited By ~ 1

Author(s):

Dini Permata Sari ◽

Mohammad Basyuni ◽

Poppy Anjelisa Zaitun Hasibuan ◽

Ridha Wati

Keyword(s):

Colon Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Colon Cancer Cell ◽

Cyclooxygenase 2 ◽

Colon Cancer Cells ◽

Chemopreventive Agents ◽

Nypa Fruticans ◽

Diphenyltetrazolium Bromide ◽

Cox 2

Objective: The objective of the study was to investigate the inhibitory activity of polyisoprenoids from Nypa fruticans leaves on the expression of cyclooxygenase 2 (COX-2) against colon cancer cells.Methods: Anticancer activity performed was tested by dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method on colon cancer cell WiDr. The expression of COX-2 was observed by the immunocytochemistry method.Result: Polyisoprenoids from N. fruticans leaves exhibit anticancer activity on WiDr cells through inhibition of COX-2 expression with IC50 180.186±7.16 μg/ml.Conclusions: This study showed that polyisoprenoids from N. fruticans leaves promise chemopreventive agents for colon cancer through COX-2 inhibition.

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