scholarly journals Exposure to ambient particulate matter alters the microbial composition and induces immune changes in rat lung

2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Naijian Li ◽  
Fang He ◽  
Baoling Liao ◽  
Yuming Zhou ◽  
Bing Li ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Rat Lung ◽  
Ambient Particulate Matter ◽  
Microbial Composition ◽  
Immune Changes

scholarly journals Chronic exposure to ambient particulate matter induces gut microbial dysbiosis in a rat COPD model

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Naijian Li ◽  
Zhaowei Yang ◽  
Baoling Liao ◽  
Tianhui Pan ◽  
Jinding Pu ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Chronic Exposure ◽  
Pulmonary Inflammation ◽  
Short Chain Fatty Acids ◽  
Chronic Obstructive ◽  
Rat Lung ◽  
Ambient Particulate Matter ◽  
Microbial Composition ◽  
Obstructive Pulmonary Disease ◽  
Microbial Dysbiosis

Abstract Background The role of the microbiota in the pathogenesis of chronic obstructive pulmonary disease (COPD) following exposure to ambient particulate matter (PM) is largely unknown. Methods Fifty-four male Sprague-Dawley rats were exposed to clean air, biomass fuel (BMF), or motor vehicle exhaust (MVE) for 4, 12, and 24 weeks. We performed pulmonary inflammation evaluation, morphometric measurements, and lung function analysis in rat lung at three different times points during exposure. Lung and gut microbial composition was assessed by 16S rRNA pyrosequencing. Serum lipopolysaccharide levels were measured and short-chain fatty acids in colon contents were quantified. Results After a 24-week PM exposure, rats exhibited pulmonary inflammation and pathological changes characteristic of COPD. The control and PM exposure (BMF and MVE) groups showed similar microbial diversity and composition in rat lung. However, the gut microbiota after 24 weeks PM exposure was characterized by decreased microbial richness and diversity, distinct overall microbial composition, lower levels of short-chain fatty acids, and higher serum lipopolysaccharide. Conclusion Chronic exposure to ambient particulate matter induces gut microbial dysbiosis and metabolite shifts in a rat model of chronic obstructive pulmonary disease.

scholarly journals Chronic Exposure to Ambient Particulate Matter Induces Gut Microbial Dysbiosis in a Rat COPD Model

2020 ◽  
Author(s):  
Naijian Li ◽  
Zhaowei Yang ◽  
Baoling Liao ◽  
Tianhui Pan ◽  
Jinding Pu ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Chronic Exposure ◽  
Pulmonary Inflammation ◽  
Short Chain Fatty Acids ◽  
Chronic Obstructive ◽  
Rat Lung ◽  
Ambient Particulate Matter ◽  
Microbial Composition ◽  
Obstructive Pulmonary Disease ◽  
Microbial Dysbiosis

Abstract Background: The role of the microbiota in the pathogenesis of chronic obstructive pulmonary disease (COPD) following exposure to ambient particulate matter (PM) is largely unknown.Methods: Fifty-four male Sprague-Dawley rats were exposed to clean air, biomass fuel (BMF), or motor vehicle exhaust (MVE) for 4, 12, and 24 weeks. We performed pulmonary inflammation evaluation, morphometric measurements, and lung function analysis in rat lung at three different times points during exposure. Lung and gut microbial composition was assessed by 16S rRNA pyrosequencing. Serum lipopolysaccharide levels were measured and short-chain fatty acids in colon contents were quantified.Results: After a 24-week PM exposure, rats exhibited pulmonary inflammation and pathological changes characteristic of COPD. The control and PM exposure (BMF and MVE) groups showed similar microbial diversity and composition in rat lung. However, the gut microbiota after 24 weeks PM exposure was characterized by decreased microbial richness and diversity, distinct overall microbial composition, lower levels of short-chain fatty acids, and higher serum lipopolysaccharide.Conclusion: Chronic exposure to ambient particulate matter induces gut microbial dysbiosis and metabolite shifts in a rat model of chronic obstructive pulmonary disease.

scholarly journals Chronic Exposure to Ambient Particulate Matter Induces Gut Microbial Dysbiosis in a Rat COPD Model

2020 ◽  
Author(s):  
Naijian Li ◽  
Zhaowei Yang ◽  
Baoling Liao ◽  
Tianhui Pan ◽  
Jinding Pu ◽  
...  
Keyword(s):  
Chronic Obstructive Pulmonary Disease ◽  
Particulate Matter ◽  
Pulmonary Disease ◽  
Chronic Exposure ◽  
Short Chain Fatty Acids ◽  
Chronic Obstructive ◽  
Ambient Particulate Matter ◽  
Microbial Composition ◽  
Obstructive Pulmonary Disease ◽  
Microbial Dysbiosis

Abstract Background: The role of the gut microbiota in the pathogenesis of chronic obstructive pulmonary disease following exposure to ambient particulate matter is largely unknown. We hypothesized that exposure alters gut microbial composition and metabolites and may involved in the pathogenesis of chronic obstructive pulmonary disease.Methods: Fifty-four male Sprague-Dawley rats were exposed to clean air, biomass fuel, or motor vehicle exhaust for 4, 12, and 24 weeks. Lung tissue was assessed histologically and gut microbial composition was assessed by 16S rRNA pyrosequencing. Serum lipopolysaccharide levels were measured and short-chain fatty acids in colon contents were quantified. Results: After a 24-week exposure to particulate matter, rats exhibited pulmonary inflammation and pathological changes characteristic of chronic obstructive pulmonary disease. The gut microbiome was characterized by decreased microbial richness and diversity, distinct overall microbial composition, lower levels of short-chain fatty acids, and higher serum lipopolysaccharide. Conclusion: Chronic exposure to ambient particulate matter induces gut microbial dysbiosis and metabolite shifts in a rat model of chronic obstructive pulmonary disease.

scholarly journals Chronic Exposure to Ambient Particulate Matter Induces Gut Microbial Dysbiosis in a Rat COPD Model

2020 ◽  
Author(s):  
Naijian Li ◽  
Zhaowei Yang ◽  
Baoling Liao ◽  
Tianhui Pan ◽  
Jinding Pu ◽  
...  
Keyword(s):  
Chronic Obstructive Pulmonary Disease ◽  
Particulate Matter ◽  
Pulmonary Disease ◽  
Chronic Exposure ◽  
Short Chain Fatty Acids ◽  
Chronic Obstructive ◽  
Ambient Particulate Matter ◽  
Microbial Composition ◽  
Obstructive Pulmonary Disease ◽  
Microbial Dysbiosis

Abstract Background The role of the gut microbiota in the pathogenesis of chronic obstructive pulmonary disease following exposure to ambient particulate matter is largely unknown. We hypothesized that exposure alters gut microbial composition and metabolites and is involved in the pathogenesis of chronic obstructive pulmonary disease. Methods Fifty-four male Sprague-Dawley rats were exposed to clean air, biomass fuel, or motor vehicle exhaust for 4, 12, and 24 weeks. Lung tissue was assessed histologically and gut microbial composition was assessed by 16S rRNA pyrosequencing. Serum lipopolysaccharide levels were measured and short-chain fatty acids in colon contents were quantified. Results After a 24-week exposure to particulate matter, rats exhibited pulmonary inflammation and pathological changes characteristic of chronic obstructive pulmonary disease. The gut microbiome was characterized by decreased microbial richness and diversity, distinct overall microbial composition, lower levels of short-chain fatty acids, and higher serum lipopolysaccharide. Conclusion Chronic exposure to ambient particulate matter induces gut microbial dysbiosis and metabolite shifts in a rat model of chronic obstructive pulmonary disease.

AMBIENT PARTICULATE MATTER (PM10 AND PM2.5) AIR POLLUTION IN DESNIANSKY DISTRICT IN KYIV

2017 ◽  
Vol 2017 (67) ◽  
pp. 31-37
Author(s):  
O. Turos ◽  
◽  
T. Maremukha ◽  
I. Kobzarenko ◽  
A. Petrosian ◽  
...  
Keyword(s):  
Air Pollution ◽  
Particulate Matter ◽  
Ambient Particulate Matter ◽  
Pm10 And Pm2.5

scholarly journals Effects of ambient particulate matter on a reconstructed human corneal epithelium model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ryota Ko ◽  
Masahiko Hayashi ◽  
Miho Tanaka ◽  
Tomoaki Okuda ◽  
Chiharu Nishita-Hara ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Cell Viability ◽  
Corneal Epithelium ◽  
Dependent Manner ◽  
Ambient Particulate Matter ◽  
Tissue Sections ◽  
Human Corneal Epithelium ◽  
Vitro Model ◽  
Dose Dependent

AbstractWe evaluated the effects of ambient particulate matter (PM) on the corneal epithelium using a reconstructed human corneal epithelium (HCE) model. We collected two PM size fractions [aerodynamic diameter smaller than 2.4 µm: PM0.3–2.4 and larger than 2.4 µm: PM>2.4] and exposed these tissues to PM concentrations of 1, 10, and 100 µg/mL for 24 h. After exposure, cell viability and interleukin (IL) IL-6 and IL-8 levels were determined, and haematoxylin and eosin and immunofluorescence staining of the zonula occludens-1 (ZO-1) were performed on tissue sections. In addition, the effects of a certified reference material of urban aerosols (UA; 100 µg/mL) were also examined as a reference. The viability of cells exposed to 100 μg/mL UA and PM>2.4 decreased to 76.2% ± 7.4 and 75.4% ± 16.1, respectively, whereas PM0.3–2.4 exposure had a limited effect on cell viability. These particles did not increase IL-6 and IL-8 levels significantly even though cell viability was decreased in 100 μg/mL UA and PM>2.4. ZO-1 expression was reduced in a dose-dependent manner in all groups. Reconstructed HCE could be used as an in vitro model to study the effects of environmental PM exposure on ocular surface cell viability and inflammation.

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