scholarly journals Diagnostic value of miR-186-5p for carotid artery stenosis and its predictive significance for future cerebral ischemic event

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Weibo Lv ◽  
Tao Zhang ◽  
Hongwei Zhao ◽  
Shuang He ◽  
Bingwei Li ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Carotid Artery Stenosis ◽  
Diagnostic Value ◽  
Artery Stenosis ◽  
Ischemic Event ◽  
Cerebral Ischemic

scholarly journals Circulating miR-106b-5p serves as a diagnostic biomarker for asymptomatic carotid artery stenosis and predicts the occurrence of cerebral ischemic events

2020 ◽  
Vol 25 (5) ◽  
pp. 436-442
Author(s):  
Tao Zhang ◽  
Zhiming Chen ◽  
Xiaowen Yang ◽  
Rui Fu ◽  
Jing Wang ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Predictive Value ◽  
Carotid Artery Stenosis ◽  
Diagnostic Value ◽  
Artery Stenosis ◽  
Asymptomatic Carotid ◽  
Cox Regression Analysis ◽  
Asymptomatic Carotid Artery Stenosis ◽  
Cerebral Ischemic ◽  
Ischemic Events

This study aimed to investigate the expression and diagnostic value of miR-106b-5p in asymptomatic carotid artery stenosis (CAS) patients, and further explore its predictive value for the occurrence of cerebral ischemic events (CIE). A total of 58 asymptomatic CAS cases and 61 healthy controls were recruited. Quantitative RT-PCR was applied for the measurement of the miR-106b-5p level. The receiver operating characteristic (ROC) curve was plotted to assess the diagnostic value of miR-106b-5p for CAS. Kaplan–Meier methods and Cox regression analysis were performed to assess the predictive value of miR-106b-5p for the occurrence of CIE. In patients with asymptomatic CAS, miR-106b-5p was highly expressed. The miR-106b-5p level showed a significant association with dyslipidemia, hypertension, and the degree of carotid stenosis. miR-106b-5p had a relative accuracy in differentiating patients with asymptomatic CAS from healthy individuals, with a sensitivity of 89.7% and specificity of 83.6% at the cutoff value of 0.198. Patients with high miR-106b-5p expression experienced more CIE. miR-106b-5p was highly expressed in patients with asymptomatic CAS. Our present results provide evidence for miR-106b-5p as a promising biomarker for CAS diagnosis, and for predicting the risk of future CIE in patients with asymptomatic CAS.

scholarly journals Urinary Proteomics Identifying Novel Biomarkers for the Diagnosis and Phenotyping of Carotid Artery Stenosis

2021 ◽  
Vol 8 ◽  
Author(s):  
Wei Wang ◽  
Jianqiang Wu ◽  
Peng Liu ◽  
Xiaoyue Tang ◽  
Haiyu Pang ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Risk Stratification ◽  
Carotid Artery Stenosis ◽  
Function Analysis ◽  
High Sensitivity ◽  
Diagnostic Value ◽  
Artery Stenosis ◽  
Healthy Controls ◽  
Early Screening ◽  
Diagnostic Values

Background: Carotid artery stenosis (CAS) is caused by the formation of atherosclerotic plaques inside the arterial wall and accounts for 20–30% of all strokes. The development of an early, noninvasive diagnostic method and the identification of high-risk patients for ischemic stroke is essential to the management of CAS in clinical practice.Methods: We used the data-independent acquisition (DIA) technique to conduct a urinary proteomic study in patients with CAS and healthy controls. We identified the potential diagnosis and risk stratification biomarkers of CAS. And Ingenuity pathway analysis was used for functional annotation of differentially expressed proteins (DEPs). Furthermore, receiver operating characteristic (ROC) analysis was performed to evaluate the diagnostic values of DEPs.Results: A total of 194 DEPs were identified between CAS patients and healthy controls by DIA quantification. The bioinformatics analysis showed that these DEPs were correlated with the pathogenesis of CAS. We further identified 32 DEPs in symptomatic CAS compared to asymptomatic CAS, and biological function analysis revealed that these proteins are mainly related to immune/inflammatory pathways. Finally, a biomarker panel of six proteins (ACP2, PLD3, HLA-C, GGH, CALML3, and IL2RB) exhibited potential diagnostic value in CAS and good discriminative power for differentiating symptomatic and asymptomatic CAS with high sensitivity and specificity.Conclusions: Our study identified novel potential urinary biomarkers for noninvasive early screening and risk stratification of CAS.

miR-28-5p improved carotid artery stenosis by regulating vascular smooth muscle cell proliferation and migration

Vascular ◽  
2021 ◽  
pp. 170853812110195
Author(s):  
Qiangrui Liu ◽  
Shibiao Yan ◽  
Yangyi Yuan ◽  
Shishun Ji ◽  
Long Guo
Keyword(s):  
Smooth Muscle ◽  
Carotid Artery ◽  
Carotid Artery Stenosis ◽  
Diagnostic Value ◽  
Artery Stenosis ◽  
Healthy Controls ◽  
Asymptomatic Carotid ◽  
Asymptomatic Carotid Artery Stenosis ◽  
And Migration ◽  
Proliferation And Migration

Objectives Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are involved in carotid artery stenosis. The purpose of this study was to investigate the diagnostic value of serum miR-28-5p in asymptomatic carotid artery stenosis and its regulation on the proliferation and migration of VSMCs. Methods Serum miR-28-5p levels in 65 healthy controls and 68 asymptomatic carotid artery stenosis patients were detected by qRT–PCR. The receiver-operating characteristic curve was applied to elucidate the diagnostic value of serum miR-28-5p for carotid artery stenosis patients. The specificity of miRNA targets was detected by luciferase reporter assay. CCK-8 and Transwell assay were applied to detect proliferation and migration of cells. Pearson correlation test was used to investigate the correlation between Forkhead box subclass O 1 (FOXO1) and serum miR-28-5p. Results Serum miR-28-5p was significantly reduced in asymptomatic carotid artery stenosis patients. Moreover, miR-28-5p could distinguish asymptomatic carotid artery stenosis patients from healthy controls, with sensitivity and specificity of 86.8% and 81.5%, respectively, indicating its high diagnostic value. The overexpression of miR-28-5p inhibited the proliferation and migration of VSMCs, while inhibition of miR-28-5p resulted in the opposite effect. What is more, FOXO1, a direct target of miR-28-5p, was significantly increased in asymptomatic carotid artery stenosis patients. Inhibition of miR-28-5p in VSMCs reversed the reduction of FOXO1 levels in patients. Conclusions miR-28-5p is a valuable diagnostic biomarker for asymptomatic carotid artery stenosis and can affect the proliferation and migration of VSMCs by regulating FOXO1.

scholarly journals Analysis of the diagnostic and prognostic value of miR-9-5p in carotid artery stenosis

2021 ◽  
Author(s):  
Hongxin Liu ◽  
Juan Zhou ◽  
Wei Jiang ◽  
Feng Wang
Keyword(s):  
Carotid Artery ◽  
Roc Curve ◽  
Predictive Value ◽  
Carotid Artery Stenosis ◽  
Diagnostic Value ◽  
Artery Stenosis ◽  
Expression Level ◽  
Kaplan Meier ◽  
Cerebrovascular Events ◽  
Low Expression

More and more evidence shows that microRNAs (miRNAs) play an important role in the diagnosis and prognosis of human diseases. In this study, we investigated the diagnostic value of miR-9-5p for asymptomatic carotid artery stenosis (CAS) and its predictive value for future cerebrovascular events within 5 years. A total of 88 asymptomatic CAS patients and 86 healthy individuals were recruited. The expression level of serum miR-9-5p was determined by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). The diagnostic value of miR-9-5p in CAS was assessed by a receiving operator characteristic (ROC) curve. The predictive value of miR-9-5p for the occurrence of cerebrovascular events was evaluated by the Kaplan-Meier method. The serum level of miR-9-5p was significantly decreased in asymptomatic CAS patients. ROC curve had an AUC value of 0.910, with the sensitivity and specificity of 80.7% and 87.2% at the cut-off value of 0.72, respectively. A total of 25 patients had cerebrovascular events during the 5-year follow-up, including 3 strokes and 22 transient ischemic attacks (TIAs). Kaplan-Meier survival analysis revealed that the low expression level of miR-9-5p was an independent factor closely related to the occurrence of cerebrovascular events. Serum miR-9-5p could be used as a new biomarker for the diagnosis of CAS, and the low expression of miR-9-5p is associated with poor prognosis.

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