scholarly journals Evaluation of in vitro anti-inflammatory and antibacterial potential of Crescentia cujete leaves and stem bark

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Mst. Shahnaj Parvin ◽  
Nandita Das ◽  
Nusrat Jahan ◽  
Most. Afia Akhter ◽  
Laizuman Nahar ◽  
...  
Keyword(s):  
Stem Bark ◽  
Crescentia Cujete ◽  
Anti Inflammatory ◽  
Antibacterial Potential

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF KNEMA ATTENUATA (HOOK. F. AND THOMSON) WARB ETHANOLIC STEM BARK EXTRACT IN ALBINO WISTAR RATS

2019 ◽  
pp. 330-334
Author(s):  
SUPRIYA RAJA H
Keyword(s):  
Stem Bark ◽  
Inflammatory Activity ◽  
Assay Method ◽  
Bark Extract ◽  
Raw 264.7 ◽  
Myeloperoxidase Activity ◽  
Stem Bark Extract ◽  
Anti Inflammatory

Objective: Knema attenuata (Myristicaceae), popularly known as “wild nutmeg,” is an endemic tree species from Western Ghats, which has been used in folk medicine. Conventionally, the stem bark of K. attenuata is used for treating inflammatory conditions without any scientific information available for the same. The present study was undertaken to evaluate the anti-inflammatory activity of the ethanolic stem bark extract (ESBE) of K. attenuata using in vivo and in vitro screening models. Methods: The ethanolic extract of stem bark was prepared by soxhlation, and its cytotoxicity in RAW 264.7 cell line was assessed using MTT assay method. In vivo anti-inflammatory effect of extract was estimated in rats using carrageenan-induced paw edema model and cotton pellet-induced granuloma model. The in vitro anti-inflammatory activity of the extract was evaluated by cyclooxygenase and lipoxygenase inhibition assay, estimation of myeloperoxidase activity, and determination of cellular nitrite levels in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Results: Toxic symptoms were not observed for the ESBE. The extract demonstrated significant anti-inflammatory activity in both in vivo and in vitro models. The anti-inflammatory action exhibited by the extract was a result of the inhibition of leukocyte migration and nitric oxide pathway and partially by inhibition of mediators such as prostaglandins and leukotrienes. Conclusion: Findings from the study provide the evidence for the popular use of stem bark extract of K. attenuata as a potential anti-inflammatory agent.

scholarly journals Virtual Screening in Pharmacokinetics, Bioactivity, and Toxicity of the Amburana cearensis Secondary Metabolites

2021 ◽  
Vol 12 (6) ◽  
pp. 8471-8491
Keyword(s):  
Secondary Metabolites ◽  
Phenolic Acids ◽  
Stem Bark ◽  
New Drugs ◽  
Structure Property ◽  
In Vivo Models ◽  
Popular Medicine ◽  
Anti Inflammatory

Bioprospecting has contributed to the work of pharmaceutical chemists in the development and commercial disposal of new drugs. Currently, the pharmaceutical industry has emphasized drugs produced from bioactive compounds extracted from natural sources, based on popular medicine discussed in the literature, such as secondary metabolites isolated from the stem bark and seeds of the Amburana cearensis, rich in coumarin derivatives, flavonoids, and phenolic acids and is popularly used in the treatment of respiratory diseases and with anti-inflammatory and antioxidant bioactivity. This review is a study of the structure/activity and structure/property (SAR/SPA) relationship with the physicochemical properties calculated by the algorithms of the MarvinSketch software for the secondary metabolites of A. cearensis, as well as their correlation with in silico test values the SwissADME and admetSAR 2.0 servers and in vitro and in vivo models of the dataset from the PreADMET, GUSAR Online and PASS Online servers. The results showed that substances derived from coumarin, flavonoids, and phenolic acids have attributes of good permeability and low efflux, which favor their oral bioavailability, since phenolic heterosides, amburoside analogs, and biflavonoids are effective in local action as subcutaneous application, constituting promising antimicrobial, anti-inflammatory and antioxidant therapeutic actions in their proper administration routes.

scholarly journals Investigation of the In Vitro Antioxidant Potential Of Polyphenolic-Rich Extract of Artocarpus heterophyllus Lam Stem Bark and Its Antidiabetic Activity In Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 25 ◽  
pp. 2515690X2091612 ◽  
Author(s):  
Basiru Olaitan Ajiboye ◽  
Oluwafemi Adeleke Ojo ◽  
Babatunji Emmanuel Oyinloye ◽  
Mary Abiola Okesola ◽  
Adeyonu Oluwatosin ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Fasting Blood Glucose ◽  
Stem Bark ◽  
Diabetic Rats ◽  
Antioxidant Potential ◽  
Antidiabetic Activity ◽  
Artocarpus Heterophyllus ◽  
Inflammatory Parameters ◽  
Anti Inflammatory

Artocarpus heterophyllus Lam (Moraceae) stem bark has been used locally in managing diabetes mellitus with sparse scientific information. This study investigates the in vitro antioxidant potential of polyphenolic-rich extract of A heterophyllus stem bark as well as its antidiabetic activity in streptozotocin-induced diabetic rats. Fifty male Wistar rats were used with the induction of diabetes by a single intraperitoneal injection of streptozotocin (45 mg/kg body weight) and were orally administered 400 mg/kg free and bound phenols of A heterophyllus stem bark. The animals were sacrificed on the 28th day of the experiment using the cervical dislocation method; antihyperglycemia and anti-inflammatory parameters were subsequently assessed. The polyphenolic extracts demonstrated antioxidant potentials (such as hydrogen peroxide and diphenyl-1-picrylhydrazyl), as well as strong inhibitory activity against amylase and glucosidase. There was a significant ( P < .05) increase in glycogen, insulin concentration, pancreatic β-cell scores (HOMA-β), antioxidant enzymes and hexokinase activities, as well as glucose transporter concentration in diabetic animals administered the extracts and metformin. Also, a significant ( P < .05) reduction in fasting blood glucose, lipid peroxidation, glucose-6-phosphatase, and all anti-inflammatory parameters were observed in diabetic rats administered the extracts and metformin. The extracts demonstrated antidiabetic potential, which may be useful in the management of diabetes mellitus

scholarly journals In vitro anti-inflammatory, anti-oxidant and in vivo anti-arthritic properties of stem bark extracts from Nauclea pobeguinii (Rubiaceae) in rats

2020 ◽  
Vol 10 (2) ◽  
pp. 65
Author(s):  
Mbiantcha Marius ◽  
Ateufack Gilbert ◽  
TsafackEric Gonzal ◽  
DjuichouNguemnang Stephanie Flore ◽  
AtsamoAlbert Donatien ◽  
...  
Keyword(s):  
Stem Bark ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Nauclea Pobeguinii

Novel saponin and benzofuran isoflavonoid with in vitro anti-inflammatory and free radical scavenging activities from the stem bark of Pterocarpus erinaceus (Poir)

2018 ◽  
Vol 28 ◽  
pp. 69-75 ◽  
Author(s):  
Paul Djouonzo Toukam ◽  
Maurice Fotsing Tagatsing ◽  
Lauve Rachel Tchokouaha Yamthe ◽  
Gakul Baishya ◽  
Nabin Chandra Barua ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Free Radical Scavenging ◽  
Pterocarpus Erinaceus ◽  
Anti Inflammatory ◽  
Free Radical Scavenging Activities

scholarly journals Maytenus macrocarpa (Ruiz & Pav.) Briq.: Phytochemistry and Pharmacological Activity

Molecules ◽  
2019 ◽  
Vol 24 (12) ◽  
pp. 2288 ◽  
Author(s):  
Milan Malaník ◽  
Jakub Treml ◽  
Veronika Rjašková ◽  
Karolina Tížková ◽  
Petra Kaucká ◽  
...  
Keyword(s):  
Traditional Medicine ◽  
Pharmacological Activity ◽  
Stem Bark ◽  
Cytotoxic Activities ◽  
Medicinal Tree ◽  
Pure Compounds ◽  
Anti Inflammatory

Maytenus macrocarpa (Celastraceae) is a tree native to Amazonia. Its roots, leaves, bark, and combinations of these are used in traditional medicine mainly to treat rheumatism and, to a lesser extent, to heal wounds and to combat bronchitis and diarrhea. To date, mainly triterpenes and dihydro-β-agarofuran sesquiterpenes were isolated from M. macrocarpa. Extracts and selected pure compounds isolated from the leaves, roots, and stem bark showed antibacterial, antiviral, antiparasitic, anti-inflammatory, and cytotoxic activities in vitro. The aim of this review is to summarize the available ethnobotanical, phytochemical, and pharmacological information about this traditional Amazonian medicinal tree, as well as to attract the attention of phytochemists and pharmacognosists to this potentially interesting source of ethnopharmaceuticals.

scholarly journals In-vitro antioxidant and anti-inflammatory activities of ethanol stem-bark extract of Blighia sapida K. D. koenig

2021 ◽  
Author(s):  
M.B. Adekola ◽  
J.O. Areola ◽  
O.F. Fagbohun ◽  
F.T. Asaolu ◽  
G.E. Ogundepo ◽  
...  
Keyword(s):  
Stem Bark ◽  
Bark Extract ◽  
Stem Bark Extract ◽  
Anti Inflammatory ◽  
Blighia Sapida

scholarly journals Evaluation of in vitro antioxidant, anti-inflammatory, anticoagulant and antiplatelet potential of Rhus mysorensis

Biomedicine ◽  
2021 ◽  
Vol 41 (4) ◽  
pp. 724-731
Author(s):  
Ghouseul Azam ◽  
Sathisha G. Jayanna ◽  
Anitha Nelliankla ◽  
Vasanthraj Boraiah ◽  
Sujatha M. Hanumegowda ◽  
...  
Keyword(s):  
Biological Activities ◽  
Anticoagulant Activity ◽  
Stem Bark ◽  
No Production ◽  
Human Beings ◽  
Column Fraction ◽  
Anti Inflammatory ◽  
Plasma Recalcification Time ◽  
Recalcification Time

Since ancient times human beings are using plant-based medicines for the treatment of various ailments, especially in the rural areas, due to their availability and affordability. Rhus mysorensis (RM) is widely used as a traditional medicine to treat various ailments. Owing to its potential medicinal value, the present study was designed to explore the in vitro antioxidant, anti-inflammatory, anticoagulant and antiplatelet properties of purified column fraction of RM. The methanol extract of stem bark powder was sequentially fractioned by solvent partitioning. The liquid methanol fraction was further fractionated by column chromatography using gradient elution. Eluted fractions were analyzed using HPLC for percentage purity and yield. The fraction with higher percentage of purity and yield was assessed for in vitro antioxidant activity by measuring SOD and GPx activities, anti-inflammatory activity by the inhibition of nitric oxide (NO) production in LPS induced RAW264.7 cells, anticoagulation by plasma recalcification time and antiplatelet activity by agonists induced platelet aggregation respectively. The antioxidant potency of column fraction (B8) revealed that, highest enzyme activities were recorded at a concentration of 320µg/ml. The enzyme activity was found to be 2.45 U/ml for SOD and 135.75 U/L for GPx respectively. Purified column fraction (B8) of RM significantly reduces the production of NO in LPS stimulated RAW 264.7 cell lines at 320????g/ml concentration with 31.90% of inhibition. The anticoagulant activity of purified fraction was determined in terms of plasma recalcification time. Interestingly, the fraction showed the most potent anticoagulant activity both in PRP and PPP as it prolonged the clotting time. The findings indicate that the stem bark of RM possesses potent antioxidant, anti-inflammatory, anticoagulant and antiplatelet activities, supporting the use of this species for treating oxidative stress-induced inflammatory diseases. Further, bioactivity guided fractionation studies to characterize and identify specific phytochemicals responsible for these biological activities are needed.

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