scholarly journals Investigation of the In Vitro Antioxidant Potential Of Polyphenolic-Rich Extract of Artocarpus heterophyllus Lam Stem Bark and Its Antidiabetic Activity In Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 25 ◽  
pp. 2515690X2091612 ◽  
Author(s):  
Basiru Olaitan Ajiboye ◽  
Oluwafemi Adeleke Ojo ◽  
Babatunji Emmanuel Oyinloye ◽  
Mary Abiola Okesola ◽  
Adeyonu Oluwatosin ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Fasting Blood Glucose ◽  
Stem Bark ◽  
Diabetic Rats ◽  
Antioxidant Potential ◽  
Antidiabetic Activity ◽  
Artocarpus Heterophyllus ◽  
Inflammatory Parameters ◽  
Anti Inflammatory

Artocarpus heterophyllus Lam (Moraceae) stem bark has been used locally in managing diabetes mellitus with sparse scientific information. This study investigates the in vitro antioxidant potential of polyphenolic-rich extract of A heterophyllus stem bark as well as its antidiabetic activity in streptozotocin-induced diabetic rats. Fifty male Wistar rats were used with the induction of diabetes by a single intraperitoneal injection of streptozotocin (45 mg/kg body weight) and were orally administered 400 mg/kg free and bound phenols of A heterophyllus stem bark. The animals were sacrificed on the 28th day of the experiment using the cervical dislocation method; antihyperglycemia and anti-inflammatory parameters were subsequently assessed. The polyphenolic extracts demonstrated antioxidant potentials (such as hydrogen peroxide and diphenyl-1-picrylhydrazyl), as well as strong inhibitory activity against amylase and glucosidase. There was a significant ( P < .05) increase in glycogen, insulin concentration, pancreatic β-cell scores (HOMA-β), antioxidant enzymes and hexokinase activities, as well as glucose transporter concentration in diabetic animals administered the extracts and metformin. Also, a significant ( P < .05) reduction in fasting blood glucose, lipid peroxidation, glucose-6-phosphatase, and all anti-inflammatory parameters were observed in diabetic rats administered the extracts and metformin. The extracts demonstrated antidiabetic potential, which may be useful in the management of diabetes mellitus

scholarly journals In vitro, acute and subchronic evaluation of the antidiabetic activity of Atractylis flava Desf n-butanol extract in alloxan-diabetic rats

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Mohamed Akram Melakhessou ◽  
Salah Eddine Marref ◽  
Naima Benkiki ◽  
Cherine Marref ◽  
Imene Becheker ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Plant Extract ◽  
Fasting Blood Glucose ◽  
Folk Medicine ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Oral Glucose ◽  
Butanol Extract

Abstract Background Diabetes mellitus is a serious complex multifactorial disorder that imposes huge health and economic burden on societies. Because the currently available medications have many drawbacks, it's important to look for alternative therapies. Medicinal plants utilized in folk medicine are ideal candidates. Therefore, this work assessed the antidiabetic action of n-butanol extract from the whole plant Atractylis flava Desf (BEAF). These ethnomedicinal properties of BEAF were scientifically validated using in vitro and in vivo assays. In vitro antidiabetic effect of the BEAF was conducted using α-Glucosidase and α-Amylase assays. While the antihyperglycemic activity was assessed using two rat models: Alloxan-induced diabetic rats and oral glucose challenged rats. Experimental diabetes was induced by a single intraperitoneal injection of alloxan at a dose of 150 mg/kg and animals with fasting blood glucose levels (BGL) > 200 mg/dL were considered diabetic. Glibenclamide (5 mg/kg) was used as a typical drug. Results The BEAF at all tested dose levels (100, 250, and 500 mg/kg) showed a significant decrease in blood glucose level in all the two animal models. Besides, the plant extract exhibited a potent inhibitory effect on α-Amylase and α-Glucosidase activity at a concentration of 1000 µg/mL with 76.17% and 89.37%, respectively. Conclusion BEAF exerts in vitro and in vivo antidiabetic effects, these results suggest that the plant extract can be a therapeutic resource in the treatment of diabetes and hyperlipidemia.

scholarly journals RETRACTED ARTICLE: Antihyperglycemic and anti-inflammatory activities of polyphenolic-rich extract of Artocarpus heterophyllus lam stem bark in streptozotocin-induced diabetic rats

2019 ◽  
Vol 6 (1) ◽  
pp. 1649628
Author(s):  
Basiru Olaitan Ajiboye ◽  
Oluwafemi Adeleke Ojo ◽  
Babatunji Emmanuel Oyinloye ◽  
Mary Abiola Okesola ◽  
Adeyonu Oluwatosin ◽  
...  
Keyword(s):  
Stem Bark ◽  
Diabetic Rats ◽  
Artocarpus Heterophyllus ◽  
Retracted Article ◽  
Anti Inflammatory

scholarly journals Antidiabetic Studies and Dereplication of the Bioactive Constituents of Anthocleista nobilis G. Don by UHPLC/ESI-QTOF-MS

2018 ◽  
Vol 17 (1) ◽  
pp. 19-28
Author(s):  
Philip F Uzor ◽  
Charles O Nnadi ◽  
Joshua E Ahiabuike ◽  
Helen N Ani
Keyword(s):  
High Performance ◽  
Blood Glucose Concentration ◽  
Fasting Blood Glucose ◽  
Herbal Medicines ◽  
Stem Bark ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Bioactive Constituents ◽  
Traditional Use ◽  
Traditional Management

Diabetes mellitus remains a global health challenge and herbal medicines are known to be effective and safe for use in its management. Anthocleista nobilis is used in the traditional management of the disorder but this claimed use has not been validated. The present study was designed to establish and hence validate the use of the plant for the management of diabetes and also identify the bioactive phytoconstituents of the plant. The root and stem bark extracts of the plant were investigated for antidiabetic activity in alloxan-induced diabetic rats. The animals were treated orally with 100, 200 and 400 mg/kg bw of both extracts for seven days. The fasting blood glucose concentration (FBGC) of the animals was monitored for seven days with a glucometer. Ultra-high performance liquid chromatography/electrospray ionization-quadrupole time-of-flight mass spectrometric (UHPLC/ESI-QTOF-MS) technique was adopted for partial dereplication of secondary metabolites of the plant. The extracts significantly (p < 0.5) reduced the FBGC of the diabetic animals. Nine previously reported compounds in the genus Anthocleista were successfully dereplicated from the plant and identified to be scopoletin (1), anthocleistol (2), sweroside (3), djalonesone (4), fagaramide (5), lanosta-7, 24-dien-3-one (6), 1-hydroxy-3,7-dimethoxyxanthene (7), djalonensin (8) and secologanin (9). Numerous other prominent peaks were not identified and could be potential novel compounds. The traditional use of A. nobilis in the management of diabetes has been validated in the present study. The identified as well as the unidentified compounds could provide template for guided isolation of the antidiabetic and other bioactive constituents of the plant.Dhaka Univ. J. Pharm. Sci. 17(1): 19-28, 2018 (June)

scholarly journals In Vivo and In Vitro Antidiabetic and Anti-Inflammatory Properties of Flax (Linum usitatissimum L.) Seed Polyphenols

Nutrients ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 2759
Author(s):  
Hamza Mechchate ◽  
Imane Es-safi ◽  
Raffaele Conte ◽  
Christophe Hano ◽  
Amal Amaghnouje ◽  
...  
Keyword(s):  
Body Weight ◽  
Linum Usitatissimum ◽  
Fasting Blood Glucose ◽  
Antidiabetic Activity ◽  
Oral Glucose ◽  
Ratio Test ◽  
Linum Usitatissimum L ◽  
Anti Inflammatory

Flaxseed is an oilseed (45–50% oil on a dry-weight basis) crop. Its oil has demonstrated multiple health benefits and industrial applications. The goal of this research was to evaluate the antidiabetic and anti-inflammatory potential of the free polyphenol fraction of flax (Linum usitatissimum L.) seeds (PLU), based on their use in traditional medicine. Mice with alloxan-induced diabetes were used to study the antidiabetic activity of PLU in vivo, with an oral administration of 25 and 50 mg/kg over 28 days. Measurements of body weight and fasting blood glucose (FBG) were carried out weekly, and biochemical parameters were evaluated. An oral glucose tolerance test was also performed. Inhibitory activities of PLU on α-amylase and α-glucosidase activities were evaluated in vitro. The anti-inflammatory was evaluated in vivo in Wistar rats using the paw edema induction Test by carrageenan, and in vitro using the hemolysis ratio test. PLU administration to diabetic mice during the study period improved their body weight and FBG levels remarkably. In vitro inhibitory activity of digestive enzymes indicated that they may be involved in the proposed mode of action of PLU extract. Qualitative results of PLU revealed the presence of 18 polyphenols. These findings support daily consumption of flaxseed for people with diabetes, and suggest that polyphenols in flaxseed may serve as dietary supplements or novel phytomedicines to treat diabetes and its complications.

scholarly journals Comparative antidiabetic activity of methanolic extract of Operculina turpethum stem and root against healthy and streptozotocin induced diabetic rats

2012 ◽  
Vol 1 (9) ◽  
pp. 272-278 ◽  
Author(s):  
Shankaraiah Pulipaka ◽  
Srinivasa Reddy Challa ◽  
Ravindra Babu Pingili
Keyword(s):  
Diabetes Mellitus ◽  
Methanolic Extract ◽  
Fasting Blood Glucose ◽  
Pharmaceutical Journal ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Activity Levels ◽  
Glucose Levels ◽  
Normal Glucose

Diabetes mellitus (DM) consists of a group of syndromes characterized by hyperglycemia. Prevalence of both type 1 and type 2 DM is increasing worldwide, the prevalence of type 2 is rising much more rapidly because of increasing obesity and reduced activity levels as countries become more industrialized. Operculina turpethum, a medicinal plant widely used in the traditional Ayurveda and Siddha systems of medicine for the treatment of diabetes mellitus. The antidiabetic potential of the methanolic extract of O. turpethum stem (MEOTS) and methanolic extract of O. turpethum root (MEOTR) was evaluated in the Streptozotocin (STZ) - induced type 2 diabetic models. The dose 100 mg/kg of MEOTS and MEOTR were administered to normal, glucose loaded and experimental diabetic rats for 21 days. The signi?cantly (p< 0.05) reduction in fasting blood glucose levels were observed in the normal rats at 3 h as well as in the treated diabetic animals at 21 days, thereby justifying the use of the plant in the indigenous system of medicine.DOI: http://dx.doi.org/10.3329/icpj.v1i9.11618 International Current Pharmaceutical Journal 2012, 1(9): 272-278 

scholarly journals Role of TLR4/MyD88/NF-κB signaling in heart and liver-related complications in a rat model of type 2 diabetes mellitus

2021 ◽  
Vol 49 (3) ◽  
pp. 030006052199759
Author(s):  
Jiajia Tian ◽  
Yanyan Zhao ◽  
Lingling Wang ◽  
Lin Li
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Signaling Pathway ◽  
Rat Model ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Primary Response ◽  
Monocyte Chemoattractant Protein 1

Aims To analyze expression of members of the Toll-like receptor (TLR)4/myeloid differentiation primary response 88 (MyD88)/nuclear factor (NF)-κB signaling pathway in the heart and liver in a rat model of type 2 diabetes mellitus (T2DM). Our overall goal was to understand the underlying pathophysiological mechanisms. Methods We measured fasting blood glucose (FBG) and insulin (FINS) in a rat model of T2DM. Expression of members of the TLR4/MyD88/NF-κB signaling pathway as well as downstream cytokines was investigated. Levels of mRNA and protein were assessed using quantitative real-time polymerase chain reaction and western blotting, respectively. Protein content of tissue homogenates was assessed using enzyme-linked immunosorbent assays. Results Diabetic rats had lower body weights, higher FBG, higher FINS, and higher intraperitoneal glucose tolerance than normal rats. In addition, biochemical indicators related to heart and liver function were elevated in diabetic rats compared with normal rats. TLR4 and MyD88 were involved in the occurrence of T2DM as well as T2DM-related heart and liver complications. TLR4 caused T2DM-related heart and liver complications through activation of NF-κB. Conclusions TLR4/MyD88/NF-κB signaling induces production of tumor necrosis factor-α, interleukin-6, and monocyte chemoattractant protein-1, leading to the heart- and liver-related complications of T2DM.

Sequoyitol ameliorates diabetic nephropathy in diabetic rats induced with a high-fat diet and a low dose of streptozotocin

2014 ◽  
Vol 92 (5) ◽  
pp. 405-417 ◽  
Author(s):  
Xian-Wei Li ◽  
Yan Liu ◽  
Wei Hao ◽  
Jie-Ren Yang
Keyword(s):  
Diabetic Nephropathy ◽  
Blood Glucose ◽  
High Fat Diet ◽  
Low Dose ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
High Fat

Sequoyitol decreases blood glucose, improves glucose intolerance, and enhances insulin signaling in ob/ob mice. The aim of this study was to investigate the effects of sequoyitol on diabetic nephropathy in rats with type 2 diabetes mellitus and the mechanism of action. Diabetic rats, induced with a high-fat diet and a low dose of streptozotocin, and were administered sequoyitol (12.5, 25.0, and 50.0 mg·(kg body mass)−1·d−1) for 6 weeks. The levels of fasting blood glucose (FBG), serum insulin, blood urea nitrogen (BUN), and serum creatinine (SCr) were measured. The expression levels of p22phox, p47phox, NF-κB, and TGF-β1 were measured using immunohistochemisty, real-time PCR, and (or) Western blot. The total antioxidative capacity (T-AOC), as well as the levels of malondialdehyde (MDA) and reactive oxygen species (ROS) were also determined. The results showed that sequoyitol significantly decreased FBG, BUN, and SCr levels, and increased the insulin levels in diabetic rats. The level of T-AOC was significantly increased, while ROS and MDA levels and the expression of p22phox, p47phox, NF-κB, and TGF-β1 were decreased with sequoyitol treatment both in vivo and in vitro. These results suggested that sequoyitol ameliorates the progression of diabetic nephropathy in rats, as induced by a high-fat diet and a low dose of streptozotocin, through its glucose-lowering effects, antioxidant activity, and regulation of TGF-β1 expression.

scholarly journals ANTIOXIDANT PROPERTIES OF SINAPIC ACID: IN VITRO AND IN VIVO APPROACH

2017 ◽  
Vol 10 (6) ◽  
pp. 255 ◽  
Author(s):  
Nithya R ◽  
Subramanian S
Keyword(s):  
Antioxidant Properties ◽  
Intraperitoneal Administration ◽  
Radical Scavenging ◽  
Sinapic Acid ◽  
Lipid Peroxides ◽  
Diabetic Rats ◽  
Antioxidant Potential ◽  
Protein Carbonyls

Objective: This study was aimed to evaluate the antioxidant potential of sinapic acid in both in vitro and in vivo. Recently, we have reported that oral administration of sinapic acid (3,5-dimethoxy 4-hydroxycinnamic acid) an active phyto ingredient widely distributed in rye, mustard, berries, and vegetables has been shown to ameliorate hyperglycemia.Methods: Experimental Type 2 diabetes was induced in male Wistar rats by feeding high-fat diet to induce insulin resistance followed by intraperitoneal administration of a single low dose streptozotocin (35 mg/kg body weight [bw]). Sinapic acid was administered orally at a concentration of 25 mg/kg bw/rat/day for 30 days, and its efficacy was compared with metformin. In vitro, antioxidant scavenging properties of sinapic acid were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), superoxide, and nitric oxide (NO) assay.Results: Sinapic acid treatment showed a significant decline in the levels of lipid peroxides, hydroperoxides and protein carbonyls in the plasma and vital tissues of diabetic rats. The treatment also improved the antioxidant status in diabetic rats indicating the antioxidant potential of sinapic acid. In addition, the results of DPPH, ABTS, superoxide, and NO radical scavenging assays substantiate the free radical scavenging efficacy of sinapic acid.Conclusion: The results of this study evidenced that sinapic acid possess significant antioxidant properties which in turn may be responsible for its antidiabetic properties.

scholarly journals Evaluation of Antihyperglycemic potential of homeopathic medicines Insulinum, Pancreatinum and Uranium nitricum in Streptozotocin Induced diabetic Rats.

2021 ◽  
Vol 19 (3) ◽  
pp. 02-17
Author(s):  
Aditya Dilipkumar Patil ◽  
Charusheela Dilipkumar Patil ◽  
Dilipkumar Kondiba Patil
Keyword(s):  
Diabetes Mellitus ◽  
Glucose Tolerance Test ◽  
Fasting Blood Glucose ◽  
Hdl Cholesterol ◽  
Holistic Approach ◽  
Tolerance Test ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Treatment Of Diabetes

INTRODUCTION – Diabetes Mellitus is an emerging endocrine and metabolic disorder which has affected millions of people globally. Homeopathy a science of ultra-molecular doses with a holistic approach is used in treatment of Diabetes Mellitus. Homeopathic medicines are prepared from plant, mineral, sarcodes, nosodes and animal parts. Insulinum, Pancreatinum and Uranium nitricum is used in homeopathy for treatment of Diabetes Mellitus. However no any preclinical studies have been investigated for the anti-diabetic effect and its safety. METHODS – Homeopathic medicines Insulinum, Pancreatinum and Uranium nitricum in 6C (1012) dilution factor were used to examine antihyperglycemic effects in streptozotocin induced diabetic rats. After 28 days treatment bodyweight, Hematology, Biochemistry (serum glucose, urea, creatinine, SGPT, SGOT, ALP, Triglyceride and HDL-cholesterol), Oral Glucose Tolerance Test, HbA1C with histopathology of (Liver, Kidney, Pancreas) was measured. RESULTS – After Streptozotocin induction, the animals have shown significant increase in the fasting blood glucose level (p

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