scholarly journals Biocompatibility and radioprotection by newly characterized melanin pigment and its production from Dietzia schimae NM3 in optimized whey medium by response surface methodology

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Sahar Eskandari ◽  
Zahra Etemadifar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Nutrient Broth ◽  
Tyrosinase Activity ◽  
Brown Pigment ◽  
Scavenging Activity ◽  
Protection Factor ◽  
Melanin Production

Abstract Purpose To characterize and optimize the productivity of melanin using an extremotolerant actinobacterium, Dietzia schimae NM3, for the first time. Methods An extracellular brown pigment produced by D. schimae NM3 in the nutrient broth and cheese whey medium by adding L-tyrosine. The extracted melanin was analyzed by UV-visible, HPLC, and FTIR assays. The radical scavenging activity (by DPPH) and sun protection factor (SPF) of the extracted melanin were measured. The melanin cytotoxicity was assayed by MTT and chromate biosorption was measured by atomic absorption spectroscopy. Finally, melanin production by D. schimae NM3 was optimized by response surface methodology (RSM) using Box-Behnken design in the whey medium. Result The purified melanin showed similar peak to the standard melanin (SIGMA) at 3.5 min in HPLC, and C=O bands, NH2, CH, C-N, and aromatic groups by FTIR. The radical scavenging activity (by DPPH) and SPF of the extracted melanin were obtained 188.9% and 20.22, respectively. Using MTT assay, the melanin revealed non-toxic effect on the normal human fibroblast (HFB) cell culture. The melanin yield 790 mg l−1, and tyrosinase activity 3400 U ml−1 were obtained in the medium contained whey powder [5% (w v−1)], L-tyrosine 2.5 g l−1, CuSO4 0.013 g l−1, and pH 10.5, incubated at 32 °C for 3 days. The ANOVA results indicated significant P-value, model F-value, and probability, with insignificant lack of fit. After optimization with mono-factors, the nutrient broth came up with melanin yield as 1.2 g l−1 and tyrosinase activity as 4040 U ml−1. Conclusion This is the first report of melanin production by D. schimae NM3 and this natural melanin showed valuable biological properties such as high antioxidant activity and radioprotection (SPF) and the biocompatibility to human cell line.

Optimization of DPPH Radical Scavenging Activity of Exopolysaccharides from Marine P. chrysogenum HGQ6 in Submerged Fermentation Using Response Surface Methodology

2011 ◽  
Vol 140 ◽  
pp. 379-384
Author(s):  
Gan Lin Guo ◽  
Lei Guo
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Fermentation Medium ◽  
Fractional Factorial ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

The objectives of this study were to investigate the effect of fermentation medium on the DPPH radical scavenging activity of exopolysaccharides from marine Penicillium chrysogenum HGQ6 by response surface methodology (RSM). A two-level fractional factorial design was used to evaluate the effect of different components of the medium. Maltose, FeSO4, and K2HPO4 were important factors significantly affecting DPPH radical scavenging activity. These selected variables were subsequently optimized using a Box-Behnken design, and response surface analysis. The optimal medium compositions were (% w/v): maltose 2.71, FeSO4 0.0016, K2HPO4 0.1, and KNO3 1.0. Under these optimal conditions, the DPPH radical scavenging rate achieved 34.0%, which agreed with the predicted values.

Optimization of Enzyme-Assisted Extraction of Carotenoids Antioxidants from Cordyceps militaris Using Response Surface Methodology

2017 ◽  
Vol 13 (5) ◽  
Author(s):  
Bai-Juan Wang ◽  
Qing-Song Yang ◽  
Ting Chen ◽  
Xiang-Dong Qin ◽  
Jun-Rong Ma ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cordyceps Militaris ◽  
Extraction Temperature ◽  
Scavenging Activity ◽  
Assisted Extraction ◽  
Development And Utilization ◽  
Enzyme Assisted Extraction

Abstract Response surface methodology (RSM) with a Box–Behnken design (BBD) was used to optimize the extraction conditions of carotenoid antioxidants from Cordyceps militaris. An enzyme-assisted extraction (EAE) method was developed and optimized to enhance 2,2-diphenyl-1-picrylhydrazil (DPPH) radical-scavenging activity of carotenoid antioxidants from C. militaris. The optimum conditions were as follows: a pH of 4.1, an extraction time of 40.4 min, an extraction temperature of 48.7 °C and 0.43 % enzyme (cellulose: pectinase, 1:1, w/w) concentration. Under these conditions, the DDPH radical-scavenging activity of C. militaris extracts was 81.62±2.26 %, indicating that the BBD is an efficient approach to develop mathematical models for predictingcarotenoids antioxidant extraction from C. militaris. The successful extraction of carotenoid antioxidants from C. militaris provides a basis for the development and utilization of C. militaris resources.

Use of Response Surface Methodology for Phenolic Antioxidant Extraction from Lotus (Nelumbo nucifera Gaertn.) Pod Pericarp

2012 ◽  
Vol 506 ◽  
pp. 591-594 ◽  
Author(s):  
P. Pintathong ◽  
P. Chanphithak ◽  
P. Sereetaveekul ◽  
N. Thitipramote ◽  
P. Chaiwut
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phenolic Antioxidant ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

The pod of lotus (Nelumbo nucifera Gaertn.) was value-added by using as raw material for phenolic antioxidant extraction using conventional shaking method. The pericarp part of lotus pod gave the significantly highest values of extractable phenolic content (EPC), flavonoid content and DPPH radical scavenging activity which were equal to 27.15±1.74 mg gallic acid equivalent (GAE)/g dry weight (dw), 0.59±0.04 mg quercetin equivalent (QE)/g dw and 0.65±0.02 mg trolox equivalent antioxidant capacity (TEAC)/g dw, respectively. Response surface methodology (RSM) was further employed to optimize the extraction conditions of phenolic antioxidant from the pod pericarp. Three independent variables, i.e., ethanol concentration (X1), shaking speed (X2) and extraction time (X3) were investigated through two responses of EPC and DPPH radical scavenging activity. The statistical analysis indicates that the three variables and the contribution of quadratic model had significant influence on the all responses (P<0.05). The optimum extraction condition was provided as follows: ethanol concentration 77.6%, shaking speed 223.4 rpm and extraction time 5.2 h. Under the optimized condition, the predicted values of EPC and DPPH radical scavenging activity were 30.00 mg GAE/g dw and 0.74 mg TEAC/g dw, respectively. This could be hence applied in industrial phenolic antioxidant extraction of inedible pericarp of lotus pod.

OPTIMIZATION OF ULTRASONIC ASSISTED EXTRACTION OF ANTIOXIDANTS FROM MORINGA OLEIFERA LAM. LEAVES USING RESPONSE SURFACE METHODOLOGY

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (12) ◽  
pp. 32-38
Author(s):  
H. N Sholapur ◽  
◽  
F. S Dasankoppa ◽  
Z. Abbas ◽  
N.G.N. Swamy
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Moringa Oleifera ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

The present study was embarked upon for the optimization of conditions aimed at ultrasonic assisted extraction (UAE) of antioxidant chemicals from Moringa oleifera leaves by applying response surface methodology (RSM) based on face centered central composite design (CCD). Evaluation was done at three levels (20 experimental designs) with three process variables: extraction temperature (20, 30, 40oC), extraction time (20, 30, 40 min) and liquid-solid ratio (20:1, 30:1, 40:1) using probe sonicator. The extraction process optimization based on RSM was focused on the capacity of antioxidants present in the extracts to scavenge the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) free radicals. To envisage the optimization process, multiple regression analysis was carried out using RSM tool to obtain a quadratic polynomial equation. Stastical analysis of the factors and their interactions were tested using analysis of variance (ANOVA). Extraction of antioxidants was mainly influenced by liquid-solid ratio and temperature. The ideal combination of variables designed by RSM for maximum radical-scavenging activity was optimized with extracting temperature of 39.80 °C, extracting time of 39.95 min and liquidsolid ratio of 24.00 mL/g to obtain a predicted value of 85.528% of DPPH radical-scavenging activity, which was very close to the experimentally value of 86.49 % with an error of 1.12%, indicating suitability of the model.

scholarly journals Optimization of Lutein Recovery from Tetraselmis suecica by Response Surface Methodology

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 182
Author(s):  
Kang Hyun Lee ◽  
Ye Won Jang ◽  
Hansol Kim ◽  
Jang-Seu Ki ◽  
Hah Young Yoo
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
High Energy ◽  
Solid Loading ◽  
Control Group ◽  
Energy Yield ◽  
Tetraselmis Suecica ◽  
Antioxidant Power

Microalgae have been attracting attention as feedstock for biorefinery because they have various advantages, such as carbon fixation, high growth rate and high energy yield. The bioactive compounds and lutein contained in microalgae are known to be beneficial for human health, especially eye and brain health. In this study, in order to improve the recovery of bioactive extracts including lutein from Tetraselmis suecica with higher efficiency, an effective solvent was selected, and the extraction parameters such as temperature, time and solid loading were optimized by response surface methodology. The most effective solvent for lutein recovery was identified as 100% methanol, and the optimum condition was determined (42.4 °C, 4.0 h and 125 g/L biomass loading) by calculation of the multiple regression model. The maximum content of recovered lutein was found to be 2.79 mg/mL, and the ABTS radical scavenging activity (IC50) and ferric reducing antioxidant power (FRAP) value were about 3.36 mg/mL and 561.9 μmol/L, respectively. Finally, the maximum lutein recovery from T. suecica through statistical optimization was estimated to be 22.3 mg/g biomass, which was 3.1-fold improved compared to the control group.

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