scholarly journals Single cell Raman spectroscopy to identify different stages of proliferating human hepatocytes for cell therapy

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Chen Ma ◽  
Ludi Zhang ◽  
Ting He ◽  
Huiying Cao ◽  
Xiongzhao Ren ◽  
...  
Keyword(s):  
Machine Learning ◽  
Raman Spectroscopy ◽  
Discriminant Analysis ◽  
Cell Therapy ◽  
Linear Discriminant Analysis ◽  
Learning Model ◽  
Human Hepatocytes ◽  
Linear Discriminant ◽  
Analytical Technique ◽  
Machine Learning Model

Abstract Background Cell therapy provides hope for treatment of advanced liver failure. Proliferating human hepatocytes (ProliHHs) were derived from primary human hepatocytes (PHH) and as potential alternative for cell therapy in liver diseases. Due to the continuous decline of mature hepatic genes and increase of progenitor like genes during ProliHHs expanding, it is challenge to monitor the critical changes of the whole process. Raman microspectroscopy is a noninvasive, label free analytical technique with high sensitivity capacity. In this study, we evaluated the potential and feasibility to identify ProliHHs from PHH with Raman spectroscopy. Methods Raman spectra were collected at least 600 single spectrum for PHH and ProliHHs at different stages (Passage 1 to Passage 4). Linear discriminant analysis and a two-layer machine learning model were used to analyze the Raman spectroscopy data. Significant differences in Raman bands were validated by the associated conventional kits. Results Linear discriminant analysis successfully classified ProliHHs at different stages and PHH. A two-layer machine learning model was established and the overall accuracy was at 84.6%. Significant differences in Raman bands have been found within different ProliHHs cell groups, especially changes at 1003 cm−1, 1206 cm−1 and 1440 cm−1. These changes were linked with reactive oxygen species, hydroxyproline and triglyceride levels in ProliHHs, and the hypothesis were consistent with the corresponding assay results. Conclusions In brief, Raman spectroscopy was successfully employed to identify different stages of ProliHHs during dedifferentiation process. The approach can simultaneously trace multiple changes of cellular components from somatic cells to progenitor cells.

scholarly journals Single Cell Raman Spectroscopy to Identify Different Stages of Proliferating Human Hepatocytes for Cell Therapy

2021 ◽  
Author(s):  
Chen Ma ◽  
Ludi Zhang ◽  
Ting He ◽  
Huiying Cao ◽  
Chenhui Ma ◽  
...  
Keyword(s):  
Machine Learning ◽  
Raman Spectroscopy ◽  
Discriminant Analysis ◽  
Cell Therapy ◽  
Linear Discriminant Analysis ◽  
Learning Model ◽  
Human Hepatocytes ◽  
Linear Discriminant ◽  
Analytical Technique ◽  
Machine Learning Model

Abstract Background: Cell therapy provides hope for treatment of advanced liver failure. Proliferating human hepatocytes (ProliHHs) were derived from primary human hepatocytes (PHH) and as potential alternative for cell therapy in liver diseases. Due to the continuous decline of mature hepatic genes and increase of progenitor like genes during ProliHHs expanding, it is challenge to monitor the critical changes of the whole process. Raman microspectroscopy is a noninvasive, label free analytical technique with high sensitivity capacity. In this study, we evaluated the potential and feasibility to identify ProliHHs from PHH with Raman spectroscopy.Methods: Raman spectra were collected at least 600 single spectrum for PHH and ProliHHs at different stages (Passage 1 to Passage 4). Linear discriminant analysis and a two-layer machine learning model were used to analyze the Raman spectroscopy data. Significant differences in Raman bands were validated by the associated conventional kits.Results: Linear discriminant analysis successfully classified ProliHHs at different stages and PHH. A two-layer machine learning model was established and the overall accuracy was at 84.6%. Significant differences in Raman bands have been found within different ProliHHs cell groups, especially changes at 1003 cm-1, 1206 cm-1 and 1300 cm-1. These changes were linked with reactive oxygen species, hydroxyproline and triglyceride levels in ProliHHs, and the hypothesis were consistent with the corresponding assay results. Conclusions: In brief, Raman spectroscopy was successfully employed to identify different stages of ProliHHs during dedifferentiation process. The approach can simultaneously trace multiple changes of cellular components from somatic cells to progenitor cells.

scholarly journals Sex estimation in cranial remains: A comparison of machine learning and discriminant analysis in Italian populations

2020 ◽  
Author(s):  
A Pozzi ◽  
C Raffone ◽  
MG Belcastro ◽  
TL Camilleri-Carter
Keyword(s):  
Machine Learning ◽  
Discriminant Analysis ◽  
Linear Discriminant Analysis ◽  
Foramen Magnum ◽  
Sex Estimation ◽  
Learning Models ◽  
Linear Discriminant ◽  
Machine Learning Methods ◽  
Cranial Measurements ◽  
Machine Learning Models

ABSTRACTObjectivesUsing cranial measurements in two Italian populations, we compare machine learning methods to the more traditional method of linear discriminant analysis in estimating sex. We use crania in sex estimation because it is useful especially when remains are fragmented or displaced, and the cranium may be the only remains found.Materials and MethodsUsing the machine learning methods of decision tree learning, support-vector machines, k-nearest neighbor algorithm, and ensemble methods we estimate the sex of two populations: Samples from Bologna and samples from the island of Sardinia. We used two datasets, one containing 17 cranial measurements, and one measuring the foramen magnum.Results and DiscussionOur results indicate that machine learning models produce similar results to linear discriminant analysis, but in some cases machine learning produces more consistent accuracy between the sexes. Our study shows that sex can be accurately predicted (> 80%) in Italian populations using the cranial measurements we gathered, except for the foramen magnum, which shows a level of accuracy of ∼70% accurate which is on par with previous geometric morphometrics studies using crania in sex estimation. We also find that our trained machine learning models produce population-specific results; we see that Italian crania are sexually dimorphic, but the features that are important to this dimorphism differ between the populations.

scholarly journals New approaches for predicting outcomes in patients with atrial fibrillation

2020 ◽  
Vol 27 ◽  
pp. 28-32
Author(s):  
N. A. Novikova ◽  
M. Yu. Gilyarov ◽  
A. Yu. Suvorov ◽  
A. Yu. Kuchina
Keyword(s):  
Neural Network ◽  
Machine Learning ◽  
Discriminant Analysis ◽  
Linear Discriminant Analysis ◽  
Test Group ◽  
Learning Technologies ◽  
Support Vector ◽  
K Nearest Neighbor ◽  
Cardiovascular Risks ◽  
Linear Discriminant

Aim: we aimed to assess the capabilities of “machine learning” methods in predicting remote outcomes in patients with non-valvular atrial fi brillation (AF).Methods. From 2015 to 2016 234 patients with non-valvular AF were included in the study (median age 72 (65; 79) years; 50.0% men). During the median follow-up of 2.9 (2.7; 3.2) years 42 patients died, 9 patients had non-fatal acute cerebral circulatory disorders and 3 patients had non-fatal myocardial infarction (MI). These events in 52 subjects (22.2% from all patients included) were combined into a combined endpoint (death and a nonfatal cardiovascular accident at the stage of remote observation). The first 184 patients comprised a “training” group. The next 50 patients formed the “test” group. The following methods of «machine learning» were used in the analysis: classifi cation trees, linear discriminant analysis, the k-nearest neighbor method, support vectors method, neural network.Results. Long-term outcomes were influenced by age, known traditional risk factors for cardiovascular diseases, the presence of these diseases, changes in intracardiac hemodynamics and heart chambers as evaluated by echocardiography, the presence of concomitant anemia, advanced stages of chronic kidney disease, and the administration of drugs associated with a more severe cardiovascular disease progression (amiodarone, digoxin). The best prognosis was created using the model of linear discriminant analysis, the complex neural network model, and the support vector machine.Conclusion. Modern methods aimed at prognosis estimation seem to be of importance in cardiology. These methods include big data analysis and machine learning technologies. The methods require further evaluation and confirmation, and in the future they may allow correcting cardiovascular risks, using data from real clinical practice and evidence-based medicine at the same time.

scholarly journals NEW METHODS FOR PREDICTING OUTCOMES AND COMPLICATIONS IN PATIENTS WITH ATRIAL FIBRILLATION

2019 ◽  
Vol 26 (2(96)) ◽  
pp. 45-50
Author(s):  
N. A. Novikova ◽  
M. Yu. Gilyarov ◽  
A. Yu. Suvorov ◽  
A. Yu. Kuchina
Keyword(s):  
Neural Network ◽  
Machine Learning ◽  
Atrial Fibrillation ◽  
Discriminant Analysis ◽  
Linear Discriminant Analysis ◽  
Test Group ◽  
Learning Technologies ◽  
Support Vector ◽  
K Nearest Neighbor ◽  
Linear Discriminant

Aim: assessment of the capabilities of “machine learning” methods in predicting remote outcomes in patients with non-valvular atrial fibrillation (AF).Methods. From 2015 to 2016 234 patients with non-valvular AF were included in the study (median age 72 (65; 79) years; 50.0% men). During the median follow-up of 2.9 (2.7; 3.2) years 42 patients died, 9 patients had non-fatal acute cerebral circulatory disorders and 3 patients had non-fatal myocardial infarction (MI). These events in 52 subjects (22.2% from all patients included) were combined into a combined endpoint (death and a nonfatal cardiovascular accident at the stage of remote observation). The first 184 patients comprised a “training” group. The next 50 patients formed the “test” group. The following methods of «machine learning» were used in the analysis: classification trees, linear discriminant analysis, the k-nearest neighbor method, support vectors method, neural network.Results. Long-term outcomes were influenced by age, known traditional risk factors for cardiovascular diseases, the presence of these diseases, changes in intracardiac hemodynamics and heart chambers as evaluated by echocardiography, the presence of concomitant anemia, advanced stages of chronic kidney disease, and the administration of drugs associated with a more severe cardiovascular disease progression (amiodarone, digoxin). The best prognosis was created using the model of linear discriminant analysis, the complex neural network model, and the support vector machine.Conclusion. Modern methods aimed at prognosis estimation seem to be of great potential for cardiology. These methods include big data analysis and machine learning technologies. The methods require further evaluation and con firmation, and in the future they may allow correcting cardiovascular risks, using data from real clinical practice and evidence-based medicine at the same time.

scholarly journals Deteksi kavitasi menggunakan linear discriminant analysis pada pompa sentrifugal

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Berli Paripurna Kamiel ◽  
Yusuf Ahmad ◽  
Krisdiyanto Krisdiyanto
Keyword(s):  
Machine Learning ◽  
Discriminant Analysis ◽  
Linear Discriminant Analysis ◽  
Centrifugal Pump ◽  
Shape Factor ◽  
Vibration Signal ◽  
Large Contribution ◽  
Centrifugal Pumps ◽  
Linear Discriminant ◽  
The Impact

Cavitation is a phenomenon that often occurs in the centrifugal pumps. The impact of cavitation is a decrease in pump performance which will affect the ongoing production process in the industries. It is important to have a method to detect the phenomenon of cavitation early. The vibration signal is a parameter that is often used in detecting cavitation or other faulty components. One of the methods is based on the pattern recognition i.e. machine learning. Linear Discriminant Analysis (LDA) is a machine learning algorithm that has the advantage of reducing the parameters used into low dimensions without reducing the accuracy of their classification. The study proposes LDA to classify normal conditions, initial cavitation, intermediate cavitation and severe cavitation. The recording of the vibration signal is taken using the an accelerometer mounted on the inlet of the centrifugal pump. The vibration signal is then extracted using 10 statistic parameters of time domain as the LDA feature selection, namely mean, RMS, standard deviation, kurtosis, skewness, crest factor, clearance factor, shape factor, variance and peak value. The results shows that the LDA classifier can detect and classify cavitation conditions with an accuracy rate of 98.8% on training and 99.6% on testing. The shape factor, kurtosis, skewness and RMS parameters are a combination of parameters that have a large contribution to the classifier to detect and classify cavitation conditions.Keywords: Linear Discriminant Analysis (LDA), cavitation, centrifugal pump, statistical parameter

scholarly journals X-ray microtomography and linear discriminant analysis enable detection of embolism-related acoustic emissions

Plant Methods ◽  
2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Niels J. F. De Baerdemaeker ◽  
Michiel Stock ◽  
Jan Van den Bulcke ◽  
Bernard De Baets ◽  
Luc Van Hoorebeke ◽  
...  
Keyword(s):  
Machine Learning ◽  
Discriminant Analysis ◽  
Linear Discriminant Analysis ◽  
Point Contact ◽  
Vulnerability Curve ◽  
Linear Discriminant ◽  
X Ray ◽  
Species Vulnerability ◽  
Depth Analysis ◽  
Ae Signals

Abstract Background Acoustic emission (AE) sensing is in use since the late 1960s in drought-induced embolism research as a non-invasive and continuous method. It is very well suited to assess a plant’s vulnerability to dehydration. Over the last couple of years, AE sensing has further improved due to progress in AE sensors, data acquisition methods and analysis systems. Despite these recent advances, it is still challenging to detect drought-induced embolism events in the AE sources registered by the sensors during dehydration, which sometimes questions the quantitative potential of AE sensing. Results In quest of a method to separate embolism-related AE signals from other dehydration-related signals, a 2-year-old potted Fraxinus excelsior L. tree was subjected to a drought experiment. Embolism formation was acoustically measured with two broadband point-contact AE sensors while simultaneously being visualized by X-ray computed microtomography (µCT). A machine learning method was used to link visually detected embolism formation by µCT with corresponding AE signals. Specifically, applying linear discriminant analysis (LDA) on the six AE waveform parameters amplitude, counts, duration, signal strength, absolute energy and partial power in the range 100–200 kHz resulted in an embolism-related acoustic vulnerability curve (VCAE-E) better resembling the standard µCT VC (VCCT), both in time and in absolute number of embolized vessels. Interestingly, the unfiltered acoustic vulnerability curve (VCAE) also closely resembled VCCT, indicating that VCs constructed from all registered AE signals did not compromise the quantitative interpretation of the species’ vulnerability to drought-induced embolism formation. Conclusion Although machine learning could detect similar numbers of embolism-related AE as µCT, there still is insufficient model-based evidence to conclusively attribute these signals to embolism events. Future research should therefore focus on similar experiments with more in-depth analysis of acoustic waveforms, as well as explore the possibility of Fast Fourier transformation (FFT) to remove non-embolism-related AE signals.

MULTIPLE CLASSIFICATION TECHNIQUES TOWARD A ROBUST AND RELIABLE P300 BCI SYSTEM

2020 ◽  
Vol 32 (02) ◽  
pp. 2050010
Author(s):  
Fatma EL-Zahraa M. Labib ◽  
Islam A. Fouad ◽  
Mai S. Mabrouk ◽  
Amr A. Sharawy
Keyword(s):  
Machine Learning ◽  
Support Vector Machine ◽  
Discriminant Analysis ◽  
Linear Discriminant Analysis ◽  
Learning Algorithms ◽  
Machine Learning Algorithms ◽  
Support Vector ◽  
Linear Discriminant ◽  
The Brain ◽  
Bci System

A brain–computer interface (BCI) can be used for people with severe physical disabilities such as ALS or amyotrophic lateral sclerosis. BCI can allow these individuals to communicate again by creating a new communication channel directly from the brain to an output device. BCI technology can allow paralyzed people to share their intent with others, and thereby demonstrate that direct communication from the brain to the external world is possible and that it might serve useful functions. BCI systems include machine learning algorithms (MLAs). Their performance depends on the feature extraction and classification techniques employed. In this paper, we propose a system to exploit the P300 signal in the brain, a positive deflection in event-related potentials. The P300 signal can be incorporated into a spelling device. There are two benefits behind this kind of research. First of all, this work presents the research status and the advantages of communication via a BCI system, especially the P300 BCI system for disordered people, and the related literature review is presented. Secondly, the paper discusses the performance of different machine learning algorithms. Two different datasets are presented: the first dataset 2004 and the second dataset 2019. A preprocessing step is introduced to the subjects in both datasets first to extract the important features before applying the proposed machine learning methods: linear discriminant analysis (LDA I and LDA II), support vector machine (SVM I, SVM II, SVM III, and SVM IV), linear regression (LREG), Bayesian linear discriminant analysis (BLDA), and twin support vector machine (TSVM). By comparing the performance of the different machine learning systems, in the first dataset it is found that BLDA and SVMIV classifiers yield the highest performance for both subjects “A” and “B”. BLDA yields 98% and 66% for 15th and 5th sequences, respectively, whereas SVMIV yields 98% and 54.4% for 15th and 5th sequences, respectively. While in the second dataset, it is obvious that BLDA classifier yields the highest performance for both subjects “1” and “2”, it achieves 90.115%. The paper summarizes the P300 BCI system for the two introduced datasets. It discusses the proposed system, compares the classification methods performances, and considers some aspects for the future work to be handled. The results show high accuracy and less computational time which makes the system more applicable for online applications.

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