scholarly journals Microbial lipid fermentation of Trichosporon cutaneum in high saline water

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Luhuan Sun ◽  
Shuai Shao ◽  
Jie Bao
Keyword(s):  
Saline Water ◽  
Lipid Production ◽  
Yeast Cells ◽  
Trichosporon Cutaneum ◽  
Microbial Lipid ◽  
Interesting Phenomenon ◽  
Fermentative Production ◽  
Fresh Water Input ◽  
Freshwater Consumption

AbstractFermentative production of microbial lipid requires high fresh water input. The utilization of high saline seawater or industrial wastewater is an important alternative to reduce the freshwater consumption. This study revealed that oleaginous yeast Trichosporon cutaneum was tolerant to a high salinity up to 130 g/L of NaCl after long-term adaptive evolution. Lipid fermentation of T. cutaneum in seawater achieved the lipid production of 31.7 g/L with approximately 36% greater than that in freshwater. The saline water containing phenol was also tested for lipid fermentation and 23.6 g/L of lipid was produced simultaneously with the complete biodegradation of phenol. An interesting phenomenon was also observed that the yeast cells spontaneously segregated onto the upper surface of the saline water. This study extended the lipid fermentation options with practical application potentials.

scholarly journals Automatic synchronisation of the cell cycle in budding yeast through closed-loop feedback control

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Giansimone Perrino ◽  
Sara Napolitano ◽  
Francesca Galdi ◽  
Antonella La Regina ◽  
Davide Fiore ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Autonomous Vehicles ◽  
Genetic System ◽  
Yeast Cells ◽  
Control Engineering ◽  
Loop Feedback ◽  
Experimental Approaches ◽  
Closed Loop Feedback ◽  
Theoretical Foundations

AbstractThe cell cycle is the process by which eukaryotic cells replicate. Yeast cells cycle asynchronously with each cell in the population budding at a different time. Although there are several experimental approaches to synchronise cells, these usually work only in the short-term. Here, we build a cyber-genetic system to achieve long-term synchronisation of the cell population, by interfacing genetically modified yeast cells with a computer by means of microfluidics to dynamically change medium, and a microscope to estimate cell cycle phases of individual cells. The computer implements a controller algorithm to decide when, and for how long, to change the growth medium to synchronise the cell-cycle across the population. Our work builds upon solid theoretical foundations provided by Control Engineering. In addition to providing an avenue for yeast cell cycle synchronisation, our work shows that control engineering can be used to automatically steer complex biological processes towards desired behaviours similarly to what is currently done with robots and autonomous vehicles.

Microbial Lipid Production through Integrated Anaerobic-Aerobic Biotreatment Process: An Urban-Based Biorefinery Concept

2021 ◽  
Vol 9 (34) ◽  
pp. 11439-11447
Author(s):  
Emmanuel D. Revellame ◽  
Alex Zappi ◽  
Rafael Hernandez ◽  
Daniel Gang ◽  
William E. Holmes ◽  
...  
Keyword(s):  
Lipid Production ◽  
Microbial Lipid ◽  
Microbial Lipid Production

scholarly journals Cyber-yeast: Automatic synchronisation of the cell cycle in budding yeast through closed-loop feedback control

2020 ◽  
Author(s):  
Giansimone Perrino ◽  
Sara Napolitano ◽  
Francesca Galdi ◽  
Antonella La Regina ◽  
Davide Fiore ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Autonomous Vehicles ◽  
Genetic System ◽  
Yeast Cells ◽  
Control Engineering ◽  
Loop Feedback ◽  
Experimental Approaches ◽  
Closed Loop Feedback ◽  
Theoretical Foundations

ABSTRACTThe cell cycle is the process by which eukaryotic cells replicate. Yeast cells cycle asynchronously with each cell in the population budding at a different time. Although there are several experimental approaches to “synchronise” cells, these work only in the short-term. Here, we built a cyber-genetic system to achieve long-term synchronisation of the cell population, by interfacing genetically modified yeast cells with a computer by means of microfluidics to dynamically change medium, and a microscope to estimate cell cycle phases of individual cells. The computer implements a “controller” algorithm to decide when, and for how long, to change the growth medium to synchronise the cell-cycle across the population. Our work builds upon solid theoretical foundations provided by Control Engineering. In addition to providing a new avenue for yeast cell cycle synchronisation, our work shows that computers can automatically steer complex biological processes towards desired behaviours similarly to what is currently done with robots and autonomous vehicles.

scholarly journals AMPK and vacuole-associated Atg14p orchestrate μ-lipophagy for energy production and long-term survival under glucose starvation

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Arnold Y Seo ◽  
Pick-Wei Lau ◽  
Daniel Feliciano ◽  
Prabuddha Sengupta ◽  
Mark A Le Gros ◽  
...  
Keyword(s):  
Energy Source ◽  
Energy Production ◽  
Alternative Energy ◽  
Yeast Cells ◽  
Survival Strategies ◽  
Glucose Starvation ◽  
Term Survival ◽  
Long Term Survival ◽  
Glucose Depletion

Dietary restriction increases the longevity of many organisms, but the cell signaling and organellar mechanisms underlying this capability are unclear. We demonstrate that to permit long-term survival in response to sudden glucose depletion, yeast cells activate lipid-droplet (LD) consumption through micro-lipophagy (µ-lipophagy), in which fat is metabolized as an alternative energy source. AMP-activated protein kinase (AMPK) activation triggered this pathway, which required Atg14p. More gradual glucose starvation, amino acid deprivation or rapamycin did not trigger µ-lipophagy and failed to provide the needed substitute energy source for long-term survival. During acute glucose restriction, activated AMPK was stabilized from degradation and interacted with Atg14p. This prompted Atg14p redistribution from ER exit sites onto liquid-ordered vacuole membrane domains, initiating µ-lipophagy. Our findings that activated AMPK and Atg14p are required to orchestrate µ-lipophagy for energy production in starved cells is relevant for studies on aging and evolutionary survival strategies of different organisms.

scholarly journals Induction of the synthesis of an additional family of long-chain dolichols in the yeast Saccharomyces cerevisiae. Effect of starvation and ageing.

2002 ◽  
Vol 49 (3) ◽  
pp. 781-787 ◽  
Author(s):  
Anna Szkopinska ◽  
Ewa Swiezewska ◽  
Joanna Rytka
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Stationary Phase ◽  
Yeast Cells ◽  
Logarithmic Phase ◽  
Cellular Membranes ◽  
Yeast Saccharomyces Cerevisiae ◽  
Chemical Changes ◽  
Physico Chemical ◽  
Long Chain

The yeast Saccharomyces cerevisiae strain W303 synthesizes in the early logarithmic phase of growth dolichols of 14-18 isoprene residues. The analysis of the polyisoprenoids present in the stationary phase revealed an additional family which proved to be also dolichols but of 19-24 isoprene residues, constituting 39% of the total dolichols. The transfer of early logarithmic phase cells to a starvation medium lacking glucose or nitrogen resulted in the synthesis of the longer chain dolichols. The additional family of dolichols represented 13.8% and 10.3% of total dolichols in the glucose and nitrogen deficient media, respectively. The level of dolichols in yeast cells increased with the age of the cultures. Since both families of dolichols are present in stationary phase cells we postulate that the longer chain dolichols may be responsible for the physico-chemical changes in cellular membranes allowing yeast cells to adapt to nutrient deficient conditions to maintain long-term viability.

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