scholarly journals Effects of a lipid-encapsulated zinc oxide dietary supplement, on growth parameters and intestinal morphology in weanling pigs artificially infected with enterotoxigenic Escherichia coli

2015 ◽  
Vol 57 (1) ◽  
Author(s):  
Sung jae Kim ◽  
Chang Hoon Kwon ◽  
Byung Chul Park ◽  
Chul Young Lee ◽  
Jeong Hee Han
Keyword(s):  
Escherichia Coli ◽  
Zinc Oxide ◽  
Dietary Supplement ◽  
Growth Parameters ◽  
Intestinal Morphology ◽  
Enterotoxigenic Escherichia Coli ◽  
Weanling Pigs

Effects of the lipid-coated zinc oxide dietary supplement on intestinal mucosal morphology and gene expression associated with the gut health in weanling pigs challenged with enterotoxigenic Escherichia coli K88

2018 ◽  
Vol 98 (3) ◽  
pp. 538-547 ◽  
Author(s):  
Jeong Hee Han ◽  
Min Hye Song ◽  
Ha Na Kim ◽  
Insurk Jang ◽  
C. Young Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Zinc Oxide ◽  
Mrna Level ◽  
Enterotoxigenic Escherichia Coli ◽  
Mrna Levels ◽  
Gut Health ◽  
Weanling Pigs ◽  
Epithelial Barrier Function ◽  
Colony Forming Units ◽  
Mucosal Morphology

Effects of a lipid-coated zinc oxide (ZnO) Shield Zn® (SZ) vs. ZnO were evaluated. Forty 25-d-old weanling pigs were fed a nursery diet supplemented with 100 mg kg−1 Zn with ZnO (ZnO-100), ZnO-2500, SZ-100, -200, or -400. All piglets were challenged orally with 5 × 108 colony-forming units of enterotoxigenic Escherichia coli K88 on day 7 and euthanized on day 14. The fecal consistency score (FCS) was less for the SZ group vs. ZnO-100 (P < 0.05). The intestinal villus height:crypt depth ratio and goblet cell density were greater for the SZ group vs. ZnO-100. By regression analyses, SZ-100 to -200 and SZ-300 to -400 were comparable to ZnO-2500 in the FCS and intestinal variables, respectively. The jejunal mucosal mRNA level did not differ between the SZ group and either ZnO group in insulin-like growth factor-I and multiple structural proteins and cytokines including zonula occludens protein (ZO) 1 and interleukin (IL) 10 except for lower ZO-1 and IL-10 mRNA levels for the SZ group than for ZnO-2500 and ZnO-100, respectively. The ZO-1 mRNA level regressed positively on the supplemental SZ concentration. Results suggest that SZ play a role in epithelial barrier function and inflammation by modulating the expression of ZO-1 and IL-10.

Gastrointestinal ecology response of piglets’ diets containing non-starch polysaccharide hydrolysis products and egg yolk antibodies following an oral challenge with Escherichia coli (k88)

2009 ◽  
Vol 89 (3) ◽  
pp. 341-352 ◽  
Author(s):  
E Kiarie ◽  
B A Slominski ◽  
D O Krause ◽  
C M Nyachoti
Keyword(s):  
Escherichia Coli ◽  
Egg Yolk ◽  
Intestinal Morphology ◽  
Oral Challenge ◽  
Enterotoxigenic Escherichia Coli ◽  
Adaptation Period ◽  
Egg Yolk Antibodies ◽  
Hydrolysis Products ◽  
K88 Fimbriae ◽  
Polysaccharide Hydrolysis

The gastrointestinal ecology (GE) of piglets fed diets containing non-starch polysaccharide hydrolysis products (HP) and egg yolk antibodies against K88 fimbriae (EYA) following oral challenge with enterotoxigenic Escherichia coli K88 (ETEC) was investigated. The HP were products of incubating feedstuffs with a blend of carbohydrase enzymes. Forty, 21-d-old pigs (two pigs/pen) were assigned to four diets to give five pens per diet. The diets were: a control fed without or with 5 g kg-1 of HP and EYA either singly or in combination forming a 2 × 2 factorial arrangement. Following a 9-d adaptation period, all pigs were orally challenged with ETEC and killed at 24 and 48 h post-challenge (one pig/pen on each occasion). Feeding HP increased pre-challenge average daily gain (252 vs. 207 g d-1; P = 0.01). An interaction (P < 0.10) between EYA and HP was observed such that when fed in combination they resulted in higher ileal digesta lactic acid and cecal DM contents and lower ileal digesta ammonia. The main effects (P < 0.05) were such that pigs fed EYA-diets had shorter intestinal crypt whilst pigs fed HP-diets showed low gastric pH and high ileal mucosal adherent lactobacilli counts. In conclusion, HP and EYA influenced indices of fermentative characteristics and intestinal morphology in the gastrointestinal ecology of piglets orally challenged with enterotoxigenic E. coli (k88).Key words: Egg yolk antibodies, ETEC, gastrointestinal ecology, non-starch polysaccharides hydrolysis products, piglet

scholarly journals Lasso Peptide Microcin J25 Effectively Enhances Gut Barrier Function and Modulates Inflammatory Response in an Enterotoxigenic Escherichia coli-Challenged Mouse Model

2020 ◽  
Vol 21 (18) ◽  
pp. 6500 ◽  
Author(s):  
Xiuliang Ding ◽  
Haitao Yu ◽  
Shiyan Qiao
Keyword(s):  
Escherichia Coli ◽  
Barrier Function ◽  
Inflammatory Responses ◽  
Intestinal Morphology ◽  
Enterotoxigenic Escherichia Coli ◽  
Control Group ◽  
Lasso Peptide ◽  
Gut Barrier Function ◽  
Microcin J25 ◽  
Etec Infection

Bacterial resistance leads to severe public health and safety issues worldwide. Alternatives to antibiotics are currently needed. A promising lasso peptide, microcin J25 (MccJ25), is considered to be the best potential substitute for antibiotics to treat pathogen infection, including enterotoxigenic Escherichia coli (ETEC). This study evaluated the efficacy of MccJ25 in the prevention of ETEC infection. Forty-five female BALB/c mice of clean grade (aged seven weeks, approximately 16.15 g) were randomly divided into three experimental groups as follows: (i) control group (uninfected); (ii) ETEC infection group; (iii) MccJ25 + ETEC group. Fifteen mice per group in five cages, three mice/cage. MccJ25 conferred effective protection against ETEC-induced body weight loss, decrease in rectal temperature and increase in diarrhea scores in mice. Moreover, in ETEC-challenged mice model, MccJ25 significantly improved intestinal morphology, decreased intestinal histopathological scores and attenuated intestinal inflammation by decreasing proinflammatory cytokines and intestinal permeability, including reducing serum diamine oxidase and D-lactate levels. MccJ25 enhanced epithelial barrier function by increasing occludin expression in the colon and claudin-1 expression in the jejunum, ultimately improving intestinal health of host. MccJ25 was further found to alleviate gut inflammatory responses by decreasing inflammatory cytokine production and expression via the activation of the mitogen-activated protein kinase and nuclear factor κB signaling pathways. Taken together, the results indicated that MccJ25 protects against ETEC-induced intestinal injury and intestinal inflammatory responses, suggesting the potential application of MccJ25 as an excellent antimicrobial or anti-inflammation agent against pathogen infections.

scholarly journals 150 A bio-active, mineral-based feed additive improved growth performance and reduced severity of diarrhea in weanling pigs challenged with enterotoxigenic Escherichia coli

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 84-85
Author(s):  
Sue Sinn ◽  
Ran Song ◽  
Dana Beckler ◽  
Rob Musser ◽  
Kim Friesen
Keyword(s):  
Escherichia Coli ◽  
Growth Performance ◽  
Meta Analysis ◽  
Enterotoxigenic Escherichia Coli ◽  
Feed Additive ◽  
Post Inoculation ◽  
Adaptation Period ◽  
Weanling Pigs ◽  
Serum Samples ◽  
E Coli

Abstract A mineral-based feed additive, NutriQuest Protect™, was evaluated in five artificial Enterotoxigenic Escherichia coli (ETEC) challenge experiments to determine the effects on pig growth performance, fecal consistency and immune response. The five experiments were conducted following a similar procedure and utilized a total of 232 weanling pigs (19 d of age) assigned to one of three experimental treatments: non-challenged control (NC), challenged control (CC), and challenged pigs fed Protect at 4.0 g/kg (CP) with 36, 36, and 44 pens per treatment, respectively. Pharmacological ZnO or medications were not included in any diets. Pigs were allowed a 7-d adaptation period following weaning, orally inoculated with E. coli K88 or F18 on 0 d post-inoculation (dpi) and 1-dpi. Studies were concluded on 4-dpi. Pig BW and feed disappearance were measured on 0-dpi and 4-dpi. Serum samples were collected on 0 and 4-dpi to measure porcine proinflammatory cytokines. Fecal scores were measured daily over the challenge period. Data from the five experiments were compiled for meta-analysis using the MIXED procedure of SAS. The NC pigs had a greater ADG (0.09 vs. -0.01 kg/d, P = 0.002), ADFI (0.24 vs. 0.21 kg/d, P = 0.09), and final BW (6.8 vs. 6.5 kg, P < 0.05). Diarrhea frequency was significantly higher in CC pigs compared with pigs on CP and NC treatments (28.5 vs. 18.7 vs. 5.3%, P < 0.05). Results from the five experiments suggest that NutriQuest Protect™ improves growth performance and reduces inflammation and diarrhea in weaned pigs artificially challenged with E. coli K88 or F18.

Net fluid and electrolyte losses in enterotoxigenic Escherichia coli infected piglet small intestine upon perfusion with fumaric acid, zinc oxide, egg yolk antibodies or carbadox

2008 ◽  
Vol 88 (3) ◽  
pp. 485-488 ◽  
Author(s):  
E. Kiarie ◽  
D. O. Krause ◽  
C. M. Nyachoti
Keyword(s):  
Escherichia Coli ◽  
Zinc Oxide ◽  
Fumaric Acid ◽  
Egg Yolk ◽  
Enterotoxigenic Escherichia Coli ◽  
Prostaglandin E ◽  
Egg Yolk Antibodies ◽  
Intestinal Segments ◽  
K88 Fimbriae ◽  
Fluid Losses

Intestinal segments (10 per piglet) prepared in four anesthetized piglets were used to evaluate the effects of anti-diarrhea agents on net fluid and electrolyte losses and prostaglandin E2 (PGE2) concentrations upon infection with enterotoxigenic Escherichia coli (ETEC). Pairs of segments (non-infected and ETEC-infected) were perfused with either saline, fumaric acid (FA), zinc oxide (ZnO), egg yolk antibodies against K88 fimbriae (EYA) or carbadox (AB) during a 7.5-h period. Segments perfused with saline had higher (P < 0.05) net fluid and electrolyte losses compared to segments perfused with anti-diarrhea agents whilst FA showed higher fluid losses compared to other anti-diarrhea agents. Key words: Anti-diarrhea agents, enterotoxigenic Escherichia coli, piglets

scholarly journals Effect of rearing environment and dietary zinc oxide on the response of group-housed weaned pigs to enterotoxigenic Escherichia coli O149 challenge

animal ◽  
2011 ◽  
Vol 5 (8) ◽  
pp. 1170-1178 ◽  
Author(s):  
R.D. Slade ◽  
I. Kyriazakis ◽  
S.M. Carroll ◽  
F.H. Reynolds ◽  
I.J. Wellock ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Zinc Oxide ◽  
Enterotoxigenic Escherichia Coli ◽  
Dietary Zinc ◽  
Weaned Pigs ◽  
Rearing Environment
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