scholarly journals Induction of resistance in chili against Sclerotium rolfsii by plant-growth-promoting rhizobacteria and Anagallis arvensis

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Warda Sharf ◽  
Arshad Javaid ◽  
Amna Shoaib ◽  
Iqra Haider Khan
Keyword(s):  
Plant Growth ◽  
Fungal Pathogen ◽  
Sclerotium Rolfsii ◽  
Plant Growth Promoting Rhizobacteria ◽  
Positive Control ◽  
Plant Survival ◽  
Southern Blight ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Blight Disease

Abstract Background Sclerotium rolfsii is a soil-borne fungal pathogen causing diseases in more than 500 plant species. It causes southern blight disease in chili. Chemical fungicides are used to control this disease, which also pollute the environment. The present study was designed to assess the potential of two species of plant-growth-promoting rhizobacteria (PGPR) viz. Bacillus megaterium and Pseudomonas fluorescence, and an allelopathic weed, Anagallis arvensis L., for the control of southern blight disease of chili. Results Initially, three PGPR strains, viz. B. megaterium OSR3, B. megaterium ZMR6, and P. fluorescence PF-097, were selected for their in vitro antagonistic assessment against S. rolfsii by dual culture technique on potato dextrose agar medium. OSR3 showed the highest antagonistic potential (68%), followed by PF-097 (54%) and ZMR6 (33%). In a pot experiment, the two best strains of PGPR, namely OSR3 and PF-097, and dried biomass of A. arvensis (DBA) in different concentrations (1, 2 and 3%) were used to manage southern blight disease of chili. In positive control treatment (S. rolfsii only), plant survival was low (73%) than the negative control (100%). OSR3, PF-097, OSR3 + 2% DBA, and PF-097 + 2% DBA significantly enhanced plant survival over positive control. The highest increase in chili growth over positive control was recorded due to OSR3, followed by PF-097 inoculations. Contents of carotenoid and chlorophyll were significantly decreased due to the fungal pathogen and improved due to PGPR strains. Application of the two PGPR strains and different concentrations of A. arvensis distinctly increased the catalase (CAT), peroxidase (POX), and polyphenol peroxidase (PPO) activities over positive control. Conclusions The present study concluded that PGPR strains B. megaterium OSR3 and P. fluorescence PF-097 can control southern blight disease effectively and increase growth and yield of chili.

scholarly journals BIO-CONTROL ACTIVITY OF PLANT GROWTH PROMOTING RHIZOBACTERIA ON SCLEROTIUM ROLFSII

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Z. Mary Swaroopa ◽  
R. Jaya Madhuri
Keyword(s):  
Plant Growth ◽  
Agricultural Development ◽  
Crop Improvement ◽  
Sclerotium Rolfsii ◽  
Plant Growth Promoting Rhizobacteria ◽  
Crop Productivity ◽  
Chemical Components ◽  
Control Activity ◽  
Plant Growth Promoting ◽  
Growth Promoting

Crop productivity and crop improvement are colloidal components as the demand of the increasing population, worldwide for the provision of food from crops require dedicated agricultural strategies that tend to lean on natural, available and, beneficial, easily reproducible means of products. In general, the soil components rich in organic matter that can avail rich microbial community initiates agricultural productivity in abundance and in the way to deduce it. But, commercially available chemical pesticides, pollution in the environment, biotic and abiotic constituents are found to be the key components that stress the crop productivity. This can be overtaken by the microbes that can function as both “bio-fertilizer” and “antagonistic” agents, mentioned as Plant growth-promoting rhizobacteria(PGPR), as they present satisfactory, advantageous impact when ever required, due to their presence in the rhizospheric region, by providing nutrients uptake from soil and controlling the unnecessary hazardous bio-impact on plants .Present study relies on sustainable agricultural development that utilizes the bacteria from the rhizospheric region thereby recommending bio-formulation in the future to mobilize the unaware farmer for better productivity, free of devastating chemical components that enter the food chain via crop produced by using chemicals, and also by easy means without affecting the surrounding environment and human health. In this context, Sclerotium rolfsii, a deleterious pathogen that affects groundnut crops predominantly, how best can be prevented and can be suppressed by using beneficial PGPR is been studied.

Biochemical and molecular characterization of DAPG-producing plant growth-promoting rhizobacteria (PGPR) of groundnut (Arachis hypogaea L.)

2016 ◽  
Author(s):  
D. Sherathia ◽  
R. Dey ◽  
M. Thomas ◽  
T. Dalsania ◽  
K. Savsani ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Plant Growth ◽  
Fungal Pathogens ◽  
Quantitative Estimation ◽  
Sclerotium Rolfsii ◽  
Plant Growth Promoting Rhizobacteria ◽  
Growth And Yield ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth-promoting rhizobacteria (PGPR) thrive in the rhizosphere of plants and play a beneficial role in plant growth, and development along with biocontrol activities. The present study was undertaken with the aim of developing rhizobacterial inoculants for groundnut for enhancement of growth and yield and suppression of major soil-borne fungal diseases caused by Sclerotium rolfsii (stem rot) and Aspergillus niger (collar rot). Out of a total of 154 rhizobacterial isolates obtained from groundnut rhizosphere, 78 isolates were selected on the basis of in vitro antifungal activities against three major soil-borne fungal pathogens of groundnut, i.e. Aspergillus niger, Aspergillus flavus and Sclerotium rolfsii. The selected isolates were further screened for the production of 2,4-Diacetylphloroglucinol (2,4-DAPG) by the gene specific PCR amplification of phlD gene. A total of 11 rhizobacterial isolates were found to have DAPG-producing genes and selected for further studies. Gene specific primers were also used for characterization of the isolates for plant growth-promoting and biocontrol traits. The qualitative and quantitative estimation of the various attributes of the isolates were also carried out. Majority of the isolates showed production of IAA, siderophores and fluorescent pigments. The DAPG-producing rhizobacterial isolates have great potential as bio-inoculants for groundnut crop for suppressing soil-borne fungal pathogens and to enhance growth and yield.

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