scholarly journals Management of the grape mealy bug, Maconellicoccus hirsutus (Green), using entomopathogenic fungi and botanical oils: a laboratory study

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Gurav Shrishail Gopal ◽  
Balaiah Venkateshalu ◽  
A. M. Nadaf ◽  
P. N. Guru ◽  
Sateesh Pattepur
Keyword(s):  
Entomopathogenic Fungi ◽  
Cumulative Effect ◽  
Neem Oil ◽  
Standard Dosage ◽  
Maconellicoccus Hirsutus ◽  
Lecanicillium Lecanii ◽  
Minimal Concentration ◽  
Pongamia Oil ◽  
Mealy Bug ◽  
Significant Mortality

Abstract Background Laboratory experiments were conducted to evaluate the bio-efficacy and cumulative effect of entomopathogenic fungi (EPF), viz., Lecanicillium lecanii (Zimmermann) Zare and Gams, and Beauveria bassiana (Balsamo) Vuillemin and different botanical oils against grape mealy bug, Maconellicoccus hirsutus (Green). M. hirsutus infestation during berry formation has left no option other than chemical management. However, the pesticide application during this stage leads to residues. Thus, safer and sustainable biological need was tested for their effectiveness against M. hirsutus. Results Dipping bioassay technique was used to determine the cumulative mortality. When L. lecanii was used as sole (at 6 and 4 g/l), significant mortality was observed (51.63 and 50.18%). However, the maximum cumulative nymphal mortality was achieved when the combination of EPF formulations was used, i.e., 57.64% with B. bassiana + L. lecanii (6 g/l + 6 g/l). Their effectiveness was at par with the same combination but with minimal concentration, i.e., 4 g/l + 4 g/l (56.29%). Concerning the botanical oils evaluated, neem oil at 15 ml/l achieved a higher mortality of 81.36%. Consecutive effective treatment was a combination of neem oil and pongamia oil (at 10 + 10 ml/l) with 79.65% mortality. However, the standard dosage of neem oil (at 10 ml/l) and IIHR neem soap (at 10 g/l) also recorded the significant mortality of 78.09 and 77.67%, respectively. When compared, the neem oil was found significantly superior in sole and compatible combination than pongamia oil. Conclusions Lecanicillium lecanii and neem oil (sole and in combination with other compatible bioagents) proved significantly better for the management against M. hirsutus with an efficacy of 90.21 and 96.67 % mortality, respectively against the standard chemical control (more than 97%).

scholarly journals Biorational Management And Mycosis Studies Of Grape Thrips [Rhipiphorothrips cruentatus H.]

2021 ◽  
Author(s):  
Jawahar Ankireddy Reddy
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Methanolic Extract ◽  
Water Extract ◽  
Cost Benefit ◽  
Neem Oil ◽  
Lecanicillium Lecanii ◽  
Cost Benefit Ratio ◽  
Thrips Population

Field experiment was conducted to test the bioefficacy of various biorational insecticides against grapevine thrips at AICRP on Fruits, Department of Horticulture, MPKV, Rahuri, during 2017-18. Results revealed that standard check emamectin benzoate 5% SG consistently proved to be the most promising by recording the least thrips population (3.10/shoot). Among biorational insecticides neem oil 2% (4.09/shoot) proved as best treatment followed by, karanj oil (4.51/shoot) and neemazol (5.08/shoot). While entomopathogenic fungi Lecanicillium lecanii recorded less population (4.24/shoot) and emerged as best treatment as compared to the Metarhizium anisopliae (4.87/shoot) and Beauveria bassiana (5.34/shoot). However chlli methanolic extract (6.29/shoot), garlic methanolic extract (6.78/shoot), chilli water extract (6.85/shoot) and garlic water extract (7.08/shoot) are the least effective treatments. Incremental Cost Benefit Ratio (ICBR) in respect of different treatments ranged between 1.30 to 7.92. The highest ICBR of 1:7.92 was recorded in the treatment with emamectin benzoate 5 SG, and it was followed by Lecanicillium lecanii (1:6.34) and Metarhizium anisopliae (1:5.32). Although neem oil and karanj oil has great reduction of thrips population, but has less incremental cost benefit ratio i.e. 2.81 and 3.04, respectively, due to high dose and its cost. The mycosis test of three entomopathogenic fungi viz. Beauveria bassiana, Metarhizium anisopliae, Lecanicillium lecanii were studied on grape thrips. Mycosis by Beauveria bassiana was confirmed the pathogenicity of entomopathogenic fungi on grape thrips. Highly pronounced mycosis was observed by Metarhizium anisopliae on the dead bodies of thrips. Mycosis test of Lecanicillium lecanii was also proved on grape thrips (plate - 1, 2 and 3).

scholarly journals Compatibility of entomopathogenic fungi and botanicals against sucking pests of okra: an ecofriendly approach

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Jaydeep Halder ◽  
Pratap A. Divekar ◽  
A. T. Rani
Keyword(s):  
Entomopathogenic Fungi ◽  
Natural Enemies ◽  
Metarhizium Anisopliae ◽  
Chemical Control ◽  
Growth Period ◽  
Neem Oil ◽  
Lecanicillium Lecanii ◽  
Maximum Reduction ◽  
Ladybird Beetles ◽  
Micraspis Discolor

Abstract Background Okra, Abelmoschus esculentus (Linn.) Moench, is one of the most important traditional vegetables in India. The crop is attacked severely by sucking pests, viz., jassid, Amrasca biguttula biguttula Ishida, and whitefly, Bemisia tabaci Genn., throughout its growth period. To control these sucking pests, different entomopathogenic fungi (EPF), viz., Beauveria bassiana, Metarhizium anisopliae, and Lecanicillium lecanii were tested alone and their 1:1 combinations with neem oil against these sucking pests and compared to Imidacloprid 17.8% SL as chemical control under field conditions during 2018 and 2019. Results Among the EPF tested, the lowest jassid (1.16, 1.27 leaf-1) and whitefly (1.33, 0.84 leaf-1) population was recorded in plots treated with L. lecanii during the two consecutive years. Combination of L. lecanii and neem oil at half of their recommended concentrations had the lowest jassid and whitefly population among all the treatments and maximum reduction over control and at par with chemical control, i.e., Imidacloprid 17.8 SL at 0.33 ml l−1. Moreover, all these biopesticides were found relatively safe to the polyphagous predators (Micraspis discolor, Menochilus sexmaculatus, and spiders) and at par with untreated control. In contrast, Imidacloprid 17.8 SL was the most toxic among all the treatments with the lowest numbers of spiders and ladybird beetles. Conclusion Combination of the EPF like B. bassiana, M. anisopliae, and L. lecanii with neem oil at half of their recommended concentrations could be a viable ecofriendly option in the management of the sucking pests of okra, along with the conservation of natural enemies.

scholarly journals Effect of Biofertilizers and Neem Oil on the Entomopathogenic Fungi Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok

2001 ◽  
Vol 44 (4) ◽  
pp. 419-423 ◽  
Author(s):  
Edson Hirose ◽  
Pedro M. O. J. Neves ◽  
João A. C. Zequi ◽  
Luís H. Martins ◽  
Cristiane H. Peralta ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Azadirachta Indica ◽  
Organic Agriculture ◽  
Metarhizium Anisopliae ◽  
Neem Oil ◽  
Colony Diameter ◽  
Negative Effect ◽  
Fungitoxic Effect

The in vitro fungitoxic effect of three biofertilizers, E.M.-4, Multibion <FONT FACE=Symbol>Ô</FONT> and Supermagro used in organic agriculture and the neem oil (Azadirachta indica A. Juss) on the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana was studied. These products were mixed in a medium where the two fungi were inoculated, and germination, vegetative growth and conidiogenesis were assessed. The biofertilizers Supermagro and E.M.- 4 showed to be less toxic for the two fungi whereas Multibion<FONT FACE=Symbol>Ô</FONT> caused major inhibition on M. anisopliae, with reductions in germination (-37.74%), colony diameter (-30.26%) and conidiogenesis (-42.62%). Neem oil promoted a larger negative effect on B. bassiana, inhibiting germination (-45.27%), colony diameter (-36.62%) and conidiogenesis (-84.93%).

Pongamia oil soap for the management of chilli mite, Polyphagotarsonemus latus Banks and its impact on spider population

ENTOMON ◽  
2021 ◽  
Vol 46 (1) ◽  
pp. 69-72
Author(s):  
K. V. Veena Krishnan ◽  
K. M. Sreekumar
Keyword(s):  
Field Experiment ◽  
Neem Oil ◽  
Polyphagotarsonemus Latus ◽  
Pongamia Oil

Field experiment was conducted to check the efficacy of the pongamia oil soap along with Spiromesifen and Neem oil soap at different concentrations in controlling chilli mite, Polyphagotarsonemus latus and its impact on spider population. Spiromesifen was found effective against chilli mite and showed persistent action in the field, whereas pongamia oil soap reported an immediate control over the pest but its effectiveness declined with time and concentration. Among the botanicals, 3% pongamia oil soap was found effective and was followed by the 2% pongamia oil soap. Pongamia oil soap proved effective against mite up to seven days after the treatment and the effect declined by 14 days after the spray. The botanicals as well as the chemical spiromesifen were found safe to spiders in the field.

scholarly journals Amino Acids in Entomopathogenic Fungi Cultured In Vitro

Agronomy ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1899
Author(s):  
Lech Wojciech Szajdak ◽  
Stanisław Bałazy ◽  
Teresa Meysner
Keyword(s):  
Amino Acids ◽  
Entomopathogenic Fungi ◽  
Pathogenic Fungi ◽  
Individual Species ◽  
Conidiobolus Coronatus ◽  
Fungal Strains ◽  
Isaria Farinosa ◽  
Lecanicillium Lecanii ◽  
Wide Range

The content of bounded amino acids in six entomopathogenic fungi was identified and determined. Analyzing the elements characterizing the pathogenicity of individual species of fungi based on infectivity criteria, ranges of infected hosts, and the ability to induce epizootics, these can be ranked in the following order: Isaria farinosa, Isaria tenuipes, Isaria fumosorose, Lecanicillium lecanii, Conidiobolus coronatus, Isaria coleopterorum. These fungi represent two types of Hyphomycetales-Paecilomyces Bainier and Verticillium Nees ex Fr. and one type of Entomophtorales-Conidiobolus Brefeld. Our study indicates that there are significant quantitative and qualitative differences of bounded amino acids in the entomopathogenic fungal strains contained in the mycelium between high and low pathogenicity strains. The richest composition of bounded amino acids has been shown in the mycelium of the Isaria farinosa strain, which is one of the most commonly presented pathogenic fungi in this group with a very wide range of infected hosts and is the most frequently recorded in nature as an important factor limiting the population of insects.

scholarly journals Control Strategies of Papaya Mealybug, Paracoccus marginatus Williams and Willink in the Laboratory Condition

2015 ◽  
Vol 3 (4) ◽  
pp. 687-691 ◽  
Author(s):  
M.J.H. Biswas ◽  
M.A.M. Khan ◽  
K.S. Ahmed
Keyword(s):  
Laboratory Experiment ◽  
Laboratory Condition ◽  
Control Strategies ◽  
Adalia Bipunctata ◽  
Neem Oil ◽  
Ladybird Beetles ◽  
Karanja Oil ◽  
Coccinella Transversalis ◽  
Micraspis Discolor ◽  
Significant Mortality

The laboratory experiment was conducted at the laboratory of the Department of Entomology, Bangladesh Agricultural University to determine the effectiveness of polythene band, predatory ladybird beetles (available species found in Bangladesh viz. Coccinella transversalis (F.), Micraspis discolor (F.), Menochilus sexmaculatus (F.), Cyclonida sanguinea, Adalia bipunctata, Coccinella novemnotata and Illeis koebelei), four chemical insecticides and three botanical oils in controlling papaya mealybug, Paracoccus marginatus Williams and Granara de Willink. The chemical insecticides were Sevin 85 SP, Dimethoate 40 EC, Fipronil 50 EC, Deltamethrin 2.5 EC, and the botanical oils were Neem oil, Mahogany oil and Karanja oil. In the laboratory experiment, polythene banding failed to prevent the plant from the infestation of papaya mealybug. Seven commonly available species of ladybird beetles in Bangladesh did not provide any control to papaya mealybug. Among the chemical insecticides, Sevin 85 SP and Dimethoate 40 EC showed significant mortality of papaya mealybug. Deltamethrin 2.5 EC and Neem oil (2%) provided moderate control of the pest. The remaining insecticides and botanical oils were less effective to control papaya mealybug. Int J Appl Sci Biotechnol, Vol 3(4): 687-691

Bio-efficacy of Millettia pinnata oil soap in the suppression of brinjal fruit and shoot borer, Leucinodes orbonalis Guenee

ENTOMON ◽  
2021 ◽  
Vol 46 (1) ◽  
pp. 41-46
Author(s):  
P. Jeevitha ◽  
K. M. Sreekumar ◽  
B. Ramesha ◽  
C.K. Yamini Varma ◽  
N.K. Binitha
Keyword(s):  
Soap Solution ◽  
Neem Oil ◽  
Minimum Level ◽  
Shoot Borer ◽  
Leucinodes Orbonalis ◽  
Millettia Pinnata ◽  
Pongamia Oil ◽  
Fruit Damage ◽  
Chemical Pesticides ◽  
Pungam Oil

A field experiment to evaluate the efficacy of pungam (Millettia/Pongamia) oil soap at four different concentrations against brinjal fruit and shoot borer (BFSB), Leucinodes orbonalis Guenee and its effect on spiders of brinjal field revealed that application of 3% pungam oil soap brought down fruit damage to minimum level (12.94% on 7 days after third spray) followed by chlorantraniliprole 18.5% SC (0.3 mL/L), 2, 1 and 0.6% pungam oil soap and neem oil soap 0.6%. Efficacy of chlorantraniliprole persisted up to 14th day of spray followed by pungam oil soap. None of the botanical or chemical pesticides found to influence the spider population until seven days. After 14 days of application soap solution either alone or with pungam oil increased spiders over the control whereas 14 DAS it was minimum in standard check.

Evaluation of pongamia oil soap against leaf hopper, Amrasca biguttula biguttula (Ishida) infesting okra

ENTOMON ◽  
2020 ◽  
Vol 44 (4) ◽  
pp. 287-292
Author(s):  
Anu Thomas ◽  
K. M. Sreekumar
Keyword(s):  
Soap Solution ◽  
Field Studies ◽  
Reproductive Stage ◽  
Vegetative Stage ◽  
Neem Oil ◽  
Amrasca Biguttula Biguttula ◽  
Leaf Hopper ◽  
Pongamia Oil

Field studies on evaluation of pongamia and neem oil soap at various concentrations against okra leaf hopper, Amrasca biguttula biguttula (Ishida) was carried out during the year 2018- Treatments i.e. T1: Pongamia oil soap 0.6%; T2: Pongamia oil soap 1%; T3: Pongamia oil soap 2%; T4: Neem oil soap 0.6%; T5: Soap solution 0.5%; T6: Quinalphos 25 EC @ 0.05%; T7: Standard check applied once at vegetative stage and twice during reproductive stage. Quinalphos 25 EC @ 0.05 % was effective followed by pongamia oil soap 2 per cent, pongamia oil soap 1 per cent, neem oil soap 0.6 per cent and pongamia oil soap 0.6 per cent. The effectiveness of the soap reduced after seven days of treatment.

scholarly journals Pathogenicity of three commercial products of entomopathogenic fungi, Beauveria bassiana, Metarhizum anisopilae and Lecanicillium lecanii against adults of olive fly, Bactrocera oleae (Gmelin) (Diptera: Tephritidae) in the laboratory

2009 ◽  
Vol 45 (No. 3) ◽  
pp. 98-102 ◽  
Author(s):  
M.F. Mahmoud
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Oral Treatment ◽  
Bactrocera Oleae ◽  
Commercial Products ◽  
Lecanicillium Lecanii ◽  
Laboratory Conditions ◽  
Olive Fly ◽  
Mortality Response ◽  
Lethal Time

The pathogenicity of entomopathogenic fungi, <I>Beauveria bassiana, Metarhizum anisopilae</I> and <I>Lecanicillium lecanii</I>, was evaluated against adults of the olive fly <I>Bactrocera oleae</I> (Gmelin) under laboratory conditions by two ways, contact bioassays and oral bioassays. The results showed that oral bioassays caused higher mortality after four treatments than the used contact bioassays. Moreover, the virulence of<I>L. lecanii</I> was higher than the virulence of <I>B. bassiana</I> and<I> M. anisopilae</I> in both ways of experiment. Lethal time (LT<sub>50</sub>) was shorter in oral bioassays than in contact bioassays in all treatments. It was 14.67, 8.30 and 5.43 days for <I>B. bassiana, M. anisopilae</I> and<I> L. lecanii</I> with oral treatment while it was 16.6, 26.07 and 12.59 days for <I>B. bassiana, M. anisopilae</I> and<I> L. lecanii</I>, respectively, with contact treatment. The slope values were 2.41, 2.55 and 2.37 for contact bioassays and 1.64, 1.69 and 1.61 for oral bioassays of <I>B. bassiana, M. anisopilae</I>and<I> L. lecanii</I>, respectively. The mortality response to the interaction between <I>B. bassiana</I> and <I>M. anisopilae</I> was synergistic while the interaction between <I>B. bassiana + L. lecanii</I> and <I>M. anisopilae + L. lecanii </I>showed an antagonistic response.

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