scholarly journals Pathogenicity of three commercial products of entomopathogenic fungi, Beauveria bassiana, Metarhizum anisopilae and Lecanicillium lecanii against adults of olive fly, Bactrocera oleae (Gmelin) (Diptera: Tephritidae) in the laboratory

2009 ◽  
Vol 45 (No. 3) ◽  
pp. 98-102 ◽  
Author(s):  
M.F. Mahmoud
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Oral Treatment ◽  
Bactrocera Oleae ◽  
Commercial Products ◽  
Lecanicillium Lecanii ◽  
Laboratory Conditions ◽  
Olive Fly ◽  
Mortality Response ◽  
Lethal Time

The pathogenicity of entomopathogenic fungi, <I>Beauveria bassiana, Metarhizum anisopilae</I> and <I>Lecanicillium lecanii</I>, was evaluated against adults of the olive fly <I>Bactrocera oleae</I> (Gmelin) under laboratory conditions by two ways, contact bioassays and oral bioassays. The results showed that oral bioassays caused higher mortality after four treatments than the used contact bioassays. Moreover, the virulence of<I>L. lecanii</I> was higher than the virulence of <I>B. bassiana</I> and<I> M. anisopilae</I> in both ways of experiment. Lethal time (LT<sub>50</sub>) was shorter in oral bioassays than in contact bioassays in all treatments. It was 14.67, 8.30 and 5.43 days for <I>B. bassiana, M. anisopilae</I> and<I> L. lecanii</I> with oral treatment while it was 16.6, 26.07 and 12.59 days for <I>B. bassiana, M. anisopilae</I> and<I> L. lecanii</I>, respectively, with contact treatment. The slope values were 2.41, 2.55 and 2.37 for contact bioassays and 1.64, 1.69 and 1.61 for oral bioassays of <I>B. bassiana, M. anisopilae</I>and<I> L. lecanii</I>, respectively. The mortality response to the interaction between <I>B. bassiana</I> and <I>M. anisopilae</I> was synergistic while the interaction between <I>B. bassiana + L. lecanii</I> and <I>M. anisopilae + L. lecanii </I>showed an antagonistic response.

scholarly journals Evaluating the virulence of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) and Beauveria bassiana (Ascomycota: Hypocreales) isolates to Arabian rhinoceros beetle, Oryctes agamemnon arabicus

2015 ◽  
Vol 47 (3) ◽  
pp. 117 ◽  
Author(s):  
M.W. Khudhair ◽  
M.Z. Khalaf ◽  
H.F. Alrubeai ◽  
A.K. Shbar ◽  
B.S. Hamad ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Pupal Stage ◽  
Control Treatment ◽  
Rhinoceros Beetle ◽  
Oryctes Agamemnon ◽  
Lethal Time ◽  
Short Time

Virulence of entomopathogenic fungi <em>Metarhizium anisopliae</em> and <em>Beauveria bassiana</em> were tested against Arabian Rhinoceros Beetle, <em>Oryctes agamemnon arabicus</em> larvae. Four concentrations (1×10<sup>5</sup>, 1×10<sup>7</sup>, 1×10<sup>9</sup> and 1×10<sup>11</sup> conidia/mL<sup>–1</sup>) of two locally isolated entomopathogenic fungi spore suspensions were used in this study via larval direct spraying. Results revealed that both isolates can cause high mortality rate reaching 100% after 29 days. However, <em>Beauveria bassiana</em> scored higher mortality rate in short time especially at the concentration of 1×10<sup>11</sup> conidia/ mL<sup>–1</sup> with lethal time (LT)<sub>50</sub> 12.75 and LT<sub>90</sub> 20.00; while, <em>Metarhizium anisopliae</em> caused the higher percentage of malformed adults. Moreover, both isolates affected insect’s life cycle particularly in the pupal stage which was reduced remarkably by almost 50% in comparison with the control treatment.

scholarly journals Biorational Management And Mycosis Studies Of Grape Thrips [Rhipiphorothrips cruentatus H.]

2021 ◽  
Author(s):  
Jawahar Ankireddy Reddy
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Methanolic Extract ◽  
Water Extract ◽  
Cost Benefit ◽  
Neem Oil ◽  
Lecanicillium Lecanii ◽  
Cost Benefit Ratio ◽  
Thrips Population

Field experiment was conducted to test the bioefficacy of various biorational insecticides against grapevine thrips at AICRP on Fruits, Department of Horticulture, MPKV, Rahuri, during 2017-18. Results revealed that standard check emamectin benzoate 5% SG consistently proved to be the most promising by recording the least thrips population (3.10/shoot). Among biorational insecticides neem oil 2% (4.09/shoot) proved as best treatment followed by, karanj oil (4.51/shoot) and neemazol (5.08/shoot). While entomopathogenic fungi Lecanicillium lecanii recorded less population (4.24/shoot) and emerged as best treatment as compared to the Metarhizium anisopliae (4.87/shoot) and Beauveria bassiana (5.34/shoot). However chlli methanolic extract (6.29/shoot), garlic methanolic extract (6.78/shoot), chilli water extract (6.85/shoot) and garlic water extract (7.08/shoot) are the least effective treatments. Incremental Cost Benefit Ratio (ICBR) in respect of different treatments ranged between 1.30 to 7.92. The highest ICBR of 1:7.92 was recorded in the treatment with emamectin benzoate 5 SG, and it was followed by Lecanicillium lecanii (1:6.34) and Metarhizium anisopliae (1:5.32). Although neem oil and karanj oil has great reduction of thrips population, but has less incremental cost benefit ratio i.e. 2.81 and 3.04, respectively, due to high dose and its cost. The mycosis test of three entomopathogenic fungi viz. Beauveria bassiana, Metarhizium anisopliae, Lecanicillium lecanii were studied on grape thrips. Mycosis by Beauveria bassiana was confirmed the pathogenicity of entomopathogenic fungi on grape thrips. Highly pronounced mycosis was observed by Metarhizium anisopliae on the dead bodies of thrips. Mycosis test of Lecanicillium lecanii was also proved on grape thrips (plate - 1, 2 and 3).

scholarly journals Compatibility and combined efficacy of entomopathogenic fungi and neonicotinoid insecticides against Myzus persicae (Sulzer): An ecofriendly approach

2021 ◽  
Vol 30 (1) ◽  
pp. 24
Author(s):  
Jaydeep Halder ◽  
Sujan Majumder ◽  
A. B. Rai
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Myzus Persicae ◽  
Metarhizium Anisopliae ◽  
Lethal Concentration ◽  
Lipaphis Erysimi ◽  
Verticillium Lecanii ◽  
Neonicotinoid Insecticides ◽  
Test Population ◽  
Lethal Time

Efficacy of different entomopathogenic fungi (EPF) viz., Beauveria bassiana, Metarhizium anisopliae and Lecanicillium (=Verticillium) lecanii and neonicotinoid insecticides i.e., Imidacloprid, Thiamethoxam and Acetamiprid were evaluated alone and their 1:1 combination against Lipaphis erysimi prevalent in vegetable ecosystem. Among the entomopathogenic fungi, B. bassiana was found most promising registering lowest median lethal time (LT50) of 48.17, 48.92 and 48.87 h during the period of 2018, 2019 and 2020, respectively, followed by L. lecanii (49.57, 49.45 and 50.46 h), M. anisopliae (51.81, 51.67 and 51.63 h). Amongst the three neonicotinoids, Acetamiprid was found more efficacious than the Imidacloprid and Thiamethoxam. Blending of B. bassiana and Acetamiprid at half of their recommended dose took lowest (22.76, 23.48 and 23.06 h during 2018, 2019 and 2020, respectively) lethal time to kill the fifty per cent test population followed by L. lecanii + Acetamiprid (22.58, 22.68, 22.52 h) and M. anisopliae + Acetamiprid (22.61, 23.82, 23.60 h). Combinations of these entomopathogenic fungi and neonicotinoid insecticides had co-toxicity co-efficient values > 1 and lower LT50 values than each of their individual indicating the compatibility amongst them. Co-application of these EPF with sub-lethal concentration of neonicotinoids could not only be a green ecofriendly option against this sucking pest but also able to minimize the chemical insecticides load in the environment.

Evaluation of entomopathogenic fungi against Raoiella indica Hirst (Acari: Prostigmata: Tenuipalpidae)

ENTOMON ◽  
2019 ◽  
Vol 44 (3) ◽  
pp. 219-224
Author(s):  
Indhusri Chavan ◽  
S. Pradeep ◽  
S. Sridhara ◽  
K.N. Ashrith
Keyword(s):  
Laboratory Condition ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Fungal Pathogens ◽  
Environmentally Friendly ◽  
Probit Analysis ◽  
Life Stages ◽  
Leaf Discs ◽  
Lecanicillium Lecanii ◽  
Raoiella Indica

Entomopathogenic fungi Metarrhizium anisopliae, Beauveria bassiana and Lecanicillium lecanii tested against immature and adults of Raoiella indica under laboratory condition with five different concentrations of each sprayed on leaf discs containing larvae, nymphs, and adults, indicated that all life stages were susceptible. Larval and nymphal stages were generally less susceptible than adults. Based on probit analysis, L. lecanii was the most virulent with LC50 of 8.15 × 105 conidia ml-1 and 1.30 × 105 conidia ml-1 followed by M. anisopliae 18.05 x 105 conidia ml-1 and 2.70 × 105 conidia/ml and B. bassiana (27.13 × 105 conidia ml-1 and 4.80 × 105 conidia ml-1) for immature and adults, respectively. However the efficacy of the fungal pathogens evaluated clearly differs from that of the controls. These entomopathogenic fungi could be considered as an environmentally friendly alternative for biocontrol of R. indica.

scholarly journals Selecting native entomopathogenic fungi against Cosmopolites sordidus (Germar) in the laboratory

2021 ◽  
Vol 20 (1) ◽  
pp. 093-097
Author(s):  
Junior Steve Garrido Pérez ◽  
Richard Paredes-Espinosa ◽  
Giannfranco Egoávil Jump ◽  
Oniel Jeremias Aguirre Gil
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Mortality Rates ◽  
Paecilomyces Lilacinus ◽  
Cosmopolites Sordidus ◽  
Laboratory Conditions

The objective of this study was to select native isolates of entomopathogenic fungi against adult Cosmopolites sordidus in laboratory conditions. Four isolates of Beauveria bassiana, two isolates of Metarhizium anisopliae, and two isolates of Paecilomyces lilacinus were tested against C. sordidus. The entomopathogenic fungi that resulted in mortality rates over 50% were M. anisopliae (MA-CsCha and MA-Carabid) and B. bassiana (BB-CsCha and BB-CsLp). The most virulent isolates were MA-CsCha, BB-CsCha, and MA-Carabid with median lethal times (LT50) of 4.82, 5.4, and 5.79 days, respectively. In conclusion, the MA-CsCha, BB-CsCha, MA-Carabid, and BB-CsLp isolates are viable candidates to be tested in banana fields.

scholarly journals PENGGUNAAN CENDAWAN ENTOMOPATOGEN Beauveria bassiana (BALSAMO) VUILLEMIN DAN Lecanicillium lecanii (ZIMM) ZARE & GAMS UNTUK MENGENDALIKAN Helopeltis antonii SIGN (HEMIPTERA: MIRIDAE) The Use of Entomopathogenic Fungi Beauveria bassiana (Balsamo)Vuillemin

2018 ◽  
Vol 8 (3) ◽  
pp. 197-202 ◽  
Author(s):  
Sri Hastuti Anggarawati ◽  
Teguh Santoso ◽  
Ruly Anwar
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Mass Production ◽  
Low Cost ◽  
Instar Nymph ◽  
Pathology Laboratory ◽  
Tea Plantation ◽  
Lecanicillium Lecanii ◽  
Lc50 Value ◽  
Insect Pathology

Helopeltis sp. has been known as one of major pests on tea, cacao and cashew plantation. Recently, genus Helopeltis is also reported attack Acacia plantation in Sumatra and Kalimantan. For such extensive plantation, low cost biocontrol agents like entomopathogenic fungi are choosen because of the simplicity for mass production. The objective of this study was to measure the effectiveness of Beauveria bassiana and Lecanicillium lecanii against Helopeltis sp. Both fungi that were used in this study were obtained from IPB Insect Pathology Laboratory. The tested insect, Helopeltis sp. was collected from Gunung Mas tea plantation. The insects were reared in laboratory. Four level of conidial density, 109, 108, 107, 106 conidia/ mL were applied to Helopeltis adult (B. bassiana) and 3rdinstar nymph (L. lecanii). Daily mortality was observed until seven days post treatment. The result showed that L. lecanii at 106 conidia/mL caused 96.25% mortality of 3rd instar nymph of Helopeltis sp. with the LC50 value at two days observation was 1.03 x 106 conidia/ mL, LT50 was 1.198 days and LT95 was 5.25 days. On the other hand, B. bassiana at 106 conidia/ mL caused 81.25%. mortality of adult Helopeltis sp. while 100% mortality could be attained by using 108 conidia/ mL. The LC50 of B. bassiana was 3.2 x 104 conidia/ mL, at four days observation and LT50 was 4.214 days. The two fungi were thus judged effective against Helopeltis sp. in this bio assay.Key words: Beauveria bassiana, conidia, density, Helopeltis sp., Lecanicillium lecanii, mortality.

scholarly journals Effect of the native strain of the predator Nesidiocoris tenuis Reuter and the entomopathogenic fungi Beauveria bassiana and Lecanicillium muscarium against Bemisia tabaci (Genn.) under greenhouse conditions in Tunisia

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Besma Hamrouni Assadi ◽  
Sabrine Chouikhi ◽  
Refki Ettaib ◽  
Naima Boughalleb M’hamdi ◽  
Mohamed Sadok Belkadhi
Keyword(s):  
Beauveria Bassiana ◽  
Bemisia Tabaci ◽  
Entomopathogenic Fungi ◽  
Tobacco Plant ◽  
Economic Losses ◽  
Native Strain ◽  
Lecanicillium Muscarium ◽  
Southern Tunisia ◽  
Significant Efficacy ◽  
Nesidiocoris Tenuis

Abstract Background The misuse of chemical insecticides has developed the phenomenon of habituation in the whitefly Bemisia tabaci (Gennadius) causing enormous economic losses under geothermal greenhouses in southern Tunisia. Results In order to develop means of biological control appropriate to the conditions of southern Tunisia, the efficacy of the native strain of the predator Nesidiocoris tenuis Reuter (Hemiptera: Miridae) and two entomopathogenic fungi (EPF) Beauveria bassiana and Lecanicillium muscarium was tested against Bemisia tabaci (Gennadius). Indeed, the introduction of N. tenuis in doses of 1, 2, 3, or 4 nymphs per tobacco plant infested by the whitefly led to highly significant reduction in the population of B. tabaci, than the control devoid of predator. The efficacy of N. tenuis was very high against nymphs and adults of B. tabaci at all doses per plant with a rate of 98%. Likewise, B. bassiana and L. muscarium, compared to an untreated control, showed a very significant efficacy against larvae and adults of B. tabaci. In addition, the number of live nymphs of N. tenuis treated directly or introduced on nymphs of B. tabaci treated with the EPF remained relatively high, exceeding 24.8 nymphs per cage compared to the control (28.6). Conclusions It can be concluded that the native strain of N. tenuis and the EPF tested separately were effective against B. tabaci. Their combined use appears to be possible.

scholarly journals On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Shehzad ◽  
Muhammad Tariq ◽  
Tariq Mukhtar ◽  
Asim Gulzar
Keyword(s):  
Sex Ratio ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Insect Pest ◽  
Diamondback Moth ◽  
Economic Damage ◽  
Offspring Sex Ratio ◽  
Development Of Resistance

Abstract Background The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a noxious pest of cruciferous crops all over the world causing serious economic damage. Management of insect pest generally depends on chemical control; however, due to development of resistance against all types of insecticides, alternative approaches especially utilization of a microbial agent is inevitable. Results Potential of 2 entomopathogenic fungi (EPF), viz., Beauveria bassiana and Metarhizium anisopliae, was evaluated against 2nd and 3rd larval instars of P. xylostella by adopting leaf dip and direct spraying methods under laboratory conditions. Significant mortality rate was achieved by each fungus under adopted methodologies. However, B. bassiana was found to be more effective in both conditions than M. anisopliae. Highest mean corrected mortality (77.80%) was recorded, when spores of B. bassiana were sprayed on the 2nd instar larvae (LC50=1.78×104/ml) after the 6th day of treatment. Similarly, incase of M. anisopliae LC50 for the 2nd instar at the same methodology was 2.78×104/ml with a mortality percentage of 70.0%. Offspring sex ratio was non-significantly related to treatment concentration and methodology, except for the control. Conclusion Beauveria bassiana and M. anisopliae had potential to suppress P. xylostella infestations when applied appropriately. Present findings suggested that B. bassiana and M. anisopliae when sprayed on immatures of host insect had more effect as compared to leaf dip procedure. Furthermore, no significant effect of concentrations was observed on sex ratio.

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