Multispectral intravital microscopy for simultaneous bright-field and fluorescence imaging of the microvasculature

AbstractIntravital video microscopy permits the observation of microcirculatory blood flow. This often requires fluorescent probes to visualize structures and dynamic processes that cannot be observed with conventional bright-field microscopy. Conventional light microscopes do not allow for simultaneous bright-field and fluorescent imaging. Moreover, in conventional microscopes, only one type of fluorescent label can be observed. This study introduces multispectral intravital video microscopy, which combines bright-field and fluorescence microscopy in a standard light microscope. The technique enables simultaneous real-time observation of fluorescently-labeled structures in relation to their direct physical surroundings. The advancement provides context for the orientation, movement, and function of labeled structures in the microcirculation.

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Fluorescent reconstitution on deposition of PM2.5 in lung and extrapulmonary organs

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1818134116 ◽

2019 ◽

Vol 116 (7) ◽

pp. 2488-2493 ◽

Cited By ~ 25

Author(s):

Donghai Li ◽

Yongjian Li ◽

Guiling Li ◽

Yu Zhang ◽

Jiang Li ◽

...

Keyword(s):

Fine Particulate Matter ◽

Deposition Process ◽

Fluorescent Imaging ◽

Imaging Method ◽

Single Particles ◽

Deposition Pattern ◽

Fine Particulate ◽

Temporal And Spatial ◽

Time Observation ◽

Real Time Observation

The deposition of PM2.5 (fine particulate matter in air with diameter smaller than 2.5 μm) in lungs is harmful to human health. However, real-time observation on the deposition of particles in the acinar area of the lung is still a challenge in experiments. Here, a fluorescent imaging method is developed to visualize the deposition process with a high temporal and spatial resolution. The observations reveal that the deposition pattern is nonuniform, and the maximum deposition rate in the acinar area differs significantly from the prediction of the widely used average deposition model. The method is also used to find single particles in the kidney and liver, though such particles are commonly believed to be too large to enter the extrapulmonary organs.

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Real-time observation of vortex lattices in a superconductor

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100151088 ◽

1993 ◽

Vol 51 ◽

pp. 1050-1051

Author(s):

K. Harada ◽

T. Matsuda ◽

J.E. Bonevich ◽

M. Igarashi ◽

S. Kondo ◽

...

Keyword(s):

Real Time ◽

Flux Line ◽

Electron Holography ◽

Lorentz Microscopy ◽

Vortex Lattices ◽

Flux Lines ◽

Magnetic Flux Lines ◽

Time Observation ◽

Thin Superconductor ◽

Real Time Observation

Previous observations of magnetic flux-lines (vortex lattices) in superconductors, such as the field distribution of a flux-line, and flux-line dynamics activated by heat and current, have employed the high spatial resolution and magnetic sensitivity of electron holography. And recently, the 2-D static distribution of vortices was also observed by this technique. However, real-time observations of the vortex lattice, in spite of scientific and technological interest, have not been possible due to experimental difficulties. Here, we report the real-time observation of vortex lattices in a thin superconductor, by means of Lorentz microscopy using a 300 kV field emission electron microscope. This technique allows us to observe the dynamic motion of individual vortices and record the events on a VTR system.The experimental arrangement is shown in Fig. 1. A Nb thin film for transmission observation was prepared by chemical etching. The grain size of the film was increased by annealing, and single crystals were observed with a thickness of 50∼90 nm.

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Stresses and defects in the formation of a cellular pattern in directional solidification. Real-time observation by synchrotron X-ray topography on a Al-0.73 wt% Cu alloy

Journal de Physique III ◽

10.1051/jp3:1994130 ◽

1994 ◽

Vol 4 (2) ◽

pp. 293-304 ◽

Cited By ~ 11

Author(s):

G. Grange ◽

C. Jourdan ◽

J. Gastaldi ◽

B. Billia

Keyword(s):

Directional Solidification ◽

Real Time ◽

X Ray ◽

Cu Alloy ◽

Cellular Pattern ◽

Time Observation ◽

Real Time Observation

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Faculty Opinions recommendation of Real-time observation of trigger factor function on translating ribosomes.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1084835.537763 ◽

2007 ◽

Author(s):

Laura Itzhaki

Keyword(s):

Real Time ◽

Trigger Factor ◽

Time Observation ◽

Real Time Observation ◽

Factor Function

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MACHO Alert 95‐30: First Real‐Time Observation of Extended Source Effects in Gravitational Microlensing

The Astrophysical Journal ◽

10.1086/304974 ◽

1997 ◽

Vol 491 (2) ◽

pp. 436-450 ◽

Cited By ~ 119

Author(s):

C. Alcock ◽

W. H. Allen ◽

R. A. Allsman ◽

D. Alves ◽

T. S. Axelrod ◽

...

Keyword(s):

Real Time ◽

Extended Source ◽

Source Effects ◽

Gravitational Microlensing ◽

Time Observation ◽

Real Time Observation

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Real-time observation of ultrafast molecular rotation in weakly bound dimers

Physical Review Research ◽

10.1103/physrevresearch.3.023050 ◽

2021 ◽

Vol 3 (2) ◽

Author(s):

Jiaqi Zhou ◽

Chaoxiong He ◽

Ming-Ming Liu ◽

Enliang Wang ◽

Shaokui Jia ◽

...

Keyword(s):

Real Time ◽

Molecular Rotation ◽

Weakly Bound ◽

Time Observation ◽

Real Time Observation

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Real-time observation of dissipative solitons formation and breaking dynamics in a mode-locked fiber laser

Infrared Physics & Technology ◽

10.1016/j.infrared.2021.103786 ◽

2021 ◽

pp. 103786

Author(s):

Dongdong Han ◽

Yijie Wang ◽

Zhanqiang Hui ◽

Zhixing Zhang ◽

Kaili Ren ◽

...

Keyword(s):

Fiber Laser ◽

Real Time ◽

Dissipative Solitons ◽

Time Observation ◽

Real Time Observation

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Histologic Examination of a Sea Pig (Scotoplanes sp.) Using Bright Field Light Microscopy

Journal of Marine Science and Engineering ◽

10.3390/jmse9080848 ◽

2021 ◽

Vol 9 (8) ◽

pp. 848

Author(s):

Elise E. B. LaDouceur ◽

Linda A. Kuhnz ◽

Christina Biggs ◽

Alicia Bitondo ◽

Megan Olhasso ◽

...

Keyword(s):

Deep Sea ◽

Body Wall ◽

Monterey Bay ◽

Bright Field ◽

Male And Female ◽

Rete Mirabile ◽

Lateral Body ◽

Bright Field Microscopy ◽

Microscopic Findings ◽

Tube Feet

Sea pigs (Scotoplanes spp.) are deep-sea dwelling sea cucumbers of the phylum Echinodermata, class Holothuroidea, and order Elasipodida. Few reports are available on the microscopic anatomy of these deep-sea animals. This study describes the histologic findings of two, wild, male and female Scotoplanes sp. collected from Monterey Bay, California. Microscopic findings were similar to other holothuroids, with a few notable exceptions. Sea pigs were bilaterally symmetrical with six pairs of greatly enlarged tube feet arising from the lateral body wall and oriented ventrally for walking. Neither a rete mirabile nor respiratory tree was identified, and the large tube feet may function in respiration. Dorsal papillae protrude from the bivium and are histologically similar to tube feet with a large, muscular water vascular canal in the center. There were 10 buccal tentacles, the epidermis of which was highly folded. Only a single gonad was present in each animal; both male and female had histologic evidence of active gametogenesis. In the male, a presumed protozoal cyst was identified in the aboral intestinal mucosa, and was histologically similar to previous reports of coccidians. This work provides control histology for future investigations of sea pigs and related animals using bright field microscopy.

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