The Phenomenon of Chromatolysis after Resection of the Pneumogastric Nerve [Le Phenomène de la Chromatolyse après la Résection du Nerf pneumogastrique]. (Nouv. Icon. de la Salp., Nos. 4, 5, 6, 1900.) Ladame, C.

1902 ◽  
Vol 48 (200) ◽  
pp. 151-152
Author(s):  
H. J. Macevoy
Keyword(s):  
Methylene Blue ◽  
Toluidine Blue ◽  
Pneumogastric Nerve

The work is divided into six parts. In the first part, an account is given of the varying results obtained by Nissl's method of staining, concerning the minute anatomy of the nervous cell and its lesions. The numerous modifications of this method were tried, and Ladame places most reliance on the method of Van Gehuchten, which he has altered in certain details; so that, for instance, instead of washing in water after fixation he carries his specimens into 60 per cent. alcohol saturated with chloride of sodium; whence, after leaving them in this bath for a while, they are placed in 70 per cent. alcohol, also saturated with chloride of sodium. He uses, moreover, essence of cedar in preference to chloroform as the vehicle of paraffin for embedding, in spite of certain drawbacks. Toluidine blue is used instead of methylene blue for staining the sections, and gives a neat, intense coloration, agreeable to the eye; moreover it is more stable than methylene blue.

A SEM and Light Microscope Study of the Epidermal Leaf Structures of the Carnivorous Plant, Sarracenia purpurea, L.

1971 ◽  
Vol 29 ◽  
pp. 358-359
Author(s):  
B. J. Panessa ◽  
J. F. Gennaro
Keyword(s):  
Methylene Blue ◽  
Toluidine Blue ◽  
Outer Surface ◽  
Microscope Study ◽  
Light Microscope ◽  
Carnivorous Plant ◽  
Sarracenia Purpurea ◽  
Inner Surface

Tissue from the hood and sarcophagus regions were fixed in 6% glutaraldehyde in 1 M.cacodylate buffer and washed in buffer. Tissue for SEM was partially dried, attached to aluminium targets with silver conducting paint, carbon-gold coated(100-500Å), and examined in a Kent Cambridge Stereoscan S4. Tissue for the light microscope was post fixed in 1% aqueous OsO4, dehydrated in acetone (4°C), embedded in Epon 812 and sectioned at ½u on a Sorvall MT 2 ultramicrotome. Cross and longitudinal sections were cut and stained with PAS, 0.5% toluidine blue and 1% azure II-methylene blue. Measurements were made from both SEM and Light micrographs.The tissue had two structurally distinct surfaces, an outer surface with small (225-500 µ) pubescent hairs (12/mm2), numerous stoma (77/mm2), and nectar glands(8/mm2); and an inner surface with large (784-1000 µ)stiff hairs(4/mm2), fewer stoma (46/mm2) and larger, more complex glands(16/mm2), presumably of a digestive nature.

Toluidine blue and methylene blue stains in nephropathy of Fabry's disease

1980 ◽  
Vol 11 (4) ◽  
pp. 380-380 ◽  
Author(s):  
R TUBBS ◽  
G GEPHARDT ◽  
P HALL ◽  
R GIFFORDJR ◽  
J WATTS ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Toluidine Blue ◽  
Fabry’S Disease ◽  
Fabry's Disease

The photodynamic effect of methylene blue and toluidine blue on Candida albicans is dependent on medium conditions

2009 ◽  
Vol 47 (5) ◽  
pp. 619-623 ◽  
Author(s):  
Gabriela Guimarães Carvalho ◽  
Monalisa Poliana Felipe ◽  
Maricilia Silva Costa
Keyword(s):  
Methylene Blue ◽  
Candida Albicans ◽  
Toluidine Blue ◽  
Photodynamic Effect

scholarly journals Antimicrobial activity of methylene blue and toluidine blue O covalently bound to a modified silicone polymer surface

2009 ◽  
Vol 19 (34) ◽  
pp. 6167 ◽  
Author(s):  
C. Piccirillo ◽  
S. Perni ◽  
J. Gil-Thomas ◽  
P. Prokopovich ◽  
M. Wilson ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Antimicrobial Activity ◽  
Toluidine Blue ◽  
Polymer Surface ◽  
Silicone Polymer ◽  
Covalently Bound

scholarly journals IN VITRO EVALUATION OF ANTIMICROBIAL EFFICACY OF DIODE LASER WHEN USED ADJUNCTIVELY WITH SODIUM HYPOCHLORITE, METHYLENE BLUE OR TOLUIDINE BLUE IN ENTEROCOCCUS FAECALIS CONTAMINATED ROOT CANALS

2020 ◽  
pp. 148-151
Author(s):  
N. Shubhashini ◽  
Vinaya Kumar R ◽  
Annapoorna Kini ◽  
Swetha H.B. ◽  
Srikanth Choudhary B.S. ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Diode Laser ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Toluidine Blue ◽  
Antimicrobial Effect ◽  
Positive Control ◽  
Negative Control ◽  
Root Canals

AIM: To evaluate antimicrobial effect of diode laser when used adjunctively with sodium hypochlorite, methylene blue or toluidine blue in Enterococcus faecalis inoculated root canals. METHODOLOGY: 70 extracted uniradicular premolars were prepared with ProTaper files and sterilized. 5 were kept aside as negative control (G1) and the remaining 65 were inoculated with Enterococcus faecalis and incubated for 7 days. Following this, 5 were kept as positive control (G2) and the other 60 were randomly distributed into six groups: G3, diode laser irradiation (1.5W) with sodium hypochlorite; G4, diode laser (3W) with sodium hypochlorite; G5, diode laser (1.5W) with methylene blue; G6, diode laser (3W) with methylene blue; G7, diode laser (1.5W) with toluidine blue; G8, diode laser (3W) with toluidine blue. Subsequently, turbidity was assessed and CFU count determined following intracanal sampling and plating. RESULTS: G4 exhibited significantly lower mean CFUs/mL (P˂0.001) among the experimental groups. G4 demonstrated the most (98.8%) while G7 exhibited the least antibacterial effect (28.66%). Highest turbidity was observed in G5 (0.1310) while the lowest was noted in G4 (0.0287). CONCLUSION: Diode laser used adjunctively with sodium hypochlorite exhibited superior antibacterial efficacy when compared to its use with photosensitizer dyes like methylene blue and toluidine blue.

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