scholarly journals IN VITRO EVALUATION OF ANTIMICROBIAL EFFICACY OF DIODE LASER WHEN USED ADJUNCTIVELY WITH SODIUM HYPOCHLORITE, METHYLENE BLUE OR TOLUIDINE BLUE IN ENTEROCOCCUS FAECALIS CONTAMINATED ROOT CANALS

2020 ◽  
pp. 148-151
Author(s):  
N. Shubhashini ◽  
Vinaya Kumar R ◽  
Annapoorna Kini ◽  
Swetha H.B. ◽  
Srikanth Choudhary B.S. ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Diode Laser ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Toluidine Blue ◽  
Antimicrobial Effect ◽  
Positive Control ◽  
Negative Control ◽  
Root Canals

AIM: To evaluate antimicrobial effect of diode laser when used adjunctively with sodium hypochlorite, methylene blue or toluidine blue in Enterococcus faecalis inoculated root canals. METHODOLOGY: 70 extracted uniradicular premolars were prepared with ProTaper files and sterilized. 5 were kept aside as negative control (G1) and the remaining 65 were inoculated with Enterococcus faecalis and incubated for 7 days. Following this, 5 were kept as positive control (G2) and the other 60 were randomly distributed into six groups: G3, diode laser irradiation (1.5W) with sodium hypochlorite; G4, diode laser (3W) with sodium hypochlorite; G5, diode laser (1.5W) with methylene blue; G6, diode laser (3W) with methylene blue; G7, diode laser (1.5W) with toluidine blue; G8, diode laser (3W) with toluidine blue. Subsequently, turbidity was assessed and CFU count determined following intracanal sampling and plating. RESULTS: G4 exhibited significantly lower mean CFUs/mL (P˂0.001) among the experimental groups. G4 demonstrated the most (98.8%) while G7 exhibited the least antibacterial effect (28.66%). Highest turbidity was observed in G5 (0.1310) while the lowest was noted in G4 (0.0287). CONCLUSION: Diode laser used adjunctively with sodium hypochlorite exhibited superior antibacterial efficacy when compared to its use with photosensitizer dyes like methylene blue and toluidine blue.

scholarly journals In Vitro Antimicrobial Effect of a Cold Plasma Jet against Enterococcus faecalis Biofilms

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Chunqi Jiang ◽  
Christoph Schaudinn ◽  
David E. Jaramillo ◽  
Paul Webster ◽  
J. William Costerton
Keyword(s):  
Enterococcus Faecalis ◽  
Cold Plasma ◽  
Plasma Jet ◽  
Antimicrobial Effect ◽  
Bactericidal Effect ◽  
Positive Control ◽  
Negative Control ◽  
Reactive Plasma ◽  
Bacterial Cell Morphology

The hypothesis that a cold plasma jet has the antimicrobial effect against Enterococcus faecalis biofilms was tested in vitro. 27 hydroxyapatite discs were incubated with E. faecalis for six days to form a monoculture biofilm on the disc surface. The prepared substrata were divided into three groups: the negative control, the positive control (5.25% NaOCl solution), and the plasma treatment group. Resultant colony-forming unit counts were associated with observations of bacterial cell morphology changes using scanning electron microscopy (SEM). Treatment of E. faecalis biofilm with the plasma and 5.25% NaOCl for 5 min resulted in 93.1% and 90.0% kill (P<0.0001), respectively. SEM detected that nearly no intact bacteria were discernible for the plasma-exposed HA disc surfaces. The demonstrated bactericidal effect of the plasma with direct surface contact may be due to the enhanced oxidation by the locally produced reactive plasma species.

Antimicrobial Activity of Calcium Hydroxide Synthesized from Indonesian Limestone against Enterococcus faecalis

2021 ◽  
Vol 1044 ◽  
pp. 171-177
Author(s):  
Myrna Nurlatifah Zakaria ◽  
Ida Rodiyah Siti Halimah ◽  
Atia Nurul Sidiqa ◽  
Ira Artilia ◽  
Arief Cahyanto
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antimicrobial Property ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Negative Control ◽  
Root Canals ◽  
Infected Root

Recurrent endodontic infections of treated teeth are mainly linked to microbial persistency in the root canal system. Enterococcus faecalis is a bacterium that is often identified in these recurrent infections. Calcium hydroxide (Ca(OH)2) is a widely used root canal medicament and is now being developed to be produced from natural materials. Ca(OH)2, which is synthesized from natural limestone from Palimanan, Indonesia, has a structure similar to Ca(OH)2, which is often used as a root canal medicament. The purpose of this study was to determine the antimicrobial property of Ca(OH)2 paste synthesized from natural limestone compared to conventional Ca(OH)2 paste. This study is an in vitro experimental study consists of 3 groups (n=24, namely, conventional Ca(OH)2 paste, limestone synthesized Ca(OH)2 paste, and saline as the negative control, for 2 intervals of time and evaluated by Total Plate Counting (TPC) method. Samples were mandibular first premolar root canal prepared by root canal preparation and contaminated with E. faecalis (ATCC 29212). The infected root canals of each group were treated by the medicaments, and samples from the root canals were taken on day 7 (n=4) and 14 (n=4). Results showed that both Ca(OH)2 groups had antimicrobial activities against E. faecalis. The limestone Ca(OH)2 paste group had the least amount of bacterial colonies on the 7th day compared to other groups. In contrast, on the 14th day, the conventional Ca(OH)2 paste had the least bacterial colonies. It can be concluded that the Ca(OH)2 paste synthesized from natural limestone has an antimicrobial effect on E. faecalis, the antimicrobial effect was higher on the 7th day but decreases on the 14th day, whereas the conventional Ca(OH)2 had a higher antimicrobial effect on day 14 compared to day 7. Further studies are expected to improve the long-term antimicrobial effectiveness and sustainability of the natural limestone synthesized Ca(OH)2 paste.

Antibacterial Efficacy of Diode and Er:YAG Laser Irradiation in Experimentally Contaminated Primary Molar Root Canals

2009 ◽  
Vol 34 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Senem Selvi Kuvvetli ◽  
Nuket Sandalli ◽  
Nursen Topcuoglu ◽  
Guven Kulekci
Keyword(s):  
Laser Irradiation ◽  
Diode Laser ◽  
Er:Yag Laser ◽  
Positive Control ◽  
Control Group ◽  
Antibacterial Efficacy ◽  
Primary Molar ◽  
Root Canals ◽  
Number Of Bacteria

Objective: In vitro comparison of the antibacterial efficacy of Diode and Er:YAG laser irradiation with that of NaOCl irrigation in contaminated primary molar root canals. Study Design: 96 root canals prepared from 32 extracted primary molar teeth were mechanically enlarged and the teeth were randomly divided into 4 subgroups. The roots were inoculated with an overnight culture of Enterococcus faecalis in tryptic soy broth for 24 hours. The root canals irradiated with diode and Er:YAG laser and irrigated with NaOCl(5.25%) were experimental groups and untreated canals served as positive control group. Bacterial growth was analysed by counting viable E.faecalis on tryptic soy agar plates. Results: The number of bacteria was significantly reduced in experimental groups in comparison with the control group. Diode laser was determined to be more effective in reducing the number of bacteria when compared to Er:YAG laser. NaOCl irrigation was found significantly most effective. Conclusions: Diode laser irradiation and 5.25 % NaOCl application provided a significant antibacterial effect in vitro, in contaminated primary molar root canals.

scholarly journals AN INVITRO COMPARATIVE EVALUATION OF ANTICANDIDAL HERBS (GINGER & TURMERIC) ON STREPTOCOCCUS MUTANS

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Bafna Harshal P ◽  
Lunawat Sadhana R ◽  
Vathar Jagdishchandra ◽  
Kalantharakath Thanveer ◽  
Ricky Pal Singh ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Ethanolic Extract ◽  
Antimicrobial Effect ◽  
Positive Control ◽  
Negative Control ◽  
Dimethyl Formamide ◽  
Zone Of Inhibition ◽  
Ginger Extract ◽  
Inert Solvent

Aim: To comparatively evaluate the antimicrobial effect of turmeric and ginger extracts on Streptococcus mutans in in-vitro conditions. Material & Method: An in-vitro experimental study was conducted in a laboratory setting. Ethanolic extract of Ginger and Turmeric was prepared separately by cold masseration technique. The extract of each was then diluted with an inert solvent, Dimethyl Formamide, to obtain 5 different concentrations (2%, 4%, 6%, 8%, and 10%) of each. 0.2% chlorhexidine was used as a positive control and dimethyl formamide was used as negative control. The different extracts, along with controls, were then subjected to microbiological investigation to determine which gave a wider zone of inhibition against streptococcus mutans. The zone of inhibition was measured in millimeters. Results: Turmeric extracts presented the largest zone of inhibition 33mm at the concentration of 8%, while Ginger extract showed a zone of inhibition of 34mm at the concentration of 10%. Conclusion: Ethanolic extract of Turmeric demonstrated antimicrobial activity against Strptococcus Mutans at a lower concentration than that of Ginger. Keywords: Ginger, Turmeric, Streptococcus Mutans.

scholarly journals COMPARATIVE EVALUATION OF ROOT CANAL DISINFECTION USING 3% SODIUM HYPOCHLORITE, 940NM DIODE LASER AND PHOTODYNAMIC THERAPY IN SINGLE SITTING PULPECTOMY: AN IN-VIVO STUDY

2020 ◽  
pp. 1-3
Author(s):  
Thomas T Parakkal ◽  
Pulkit Jhingan ◽  
Shivani Mathur ◽  
Vinod Sachdev
Keyword(s):  
Photodynamic Therapy ◽  
Diode Laser ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Bacterial Species ◽  
Root Canals ◽  
Root Canal Disinfection ◽  
Infected Root ◽  
Colony Forming Units

Introduction: Enterococcus faecalis is a common bacterial species in resistant and recurrent infections. It has an active proton pump which prevents its easy inhibition leading to endodontic treatment failure. LASER and photodynamic therapy have been employed lately in improving antimicrobial chemotherapy of localized infections. Aim: To compare disinfection efficacy of 940nm Diode LASER & Photodynamic therapy(PDT) to Conventional method using 3% Sodium hypochlorite in canals of endodontically treated primary teeth. Method: Single sitting Pulpectomy was performed on 60 mandibular first primary molars, divided into 3 groups, disinfected using mentioned modalities. Disinfection potential was compared collecting samples at 3 stages using paper points, cultured and incubated for 24hrs to check change in Colony Forming Units of the bacteria. Results: Data analysis with SPSS version 20 software using Kruskal-Wallis and Mann Whitney-U test. Conclusion: The three groups showed effective disinfection as revealed by the reduction in the colony forming units of the bacteria at baseline and after intervention. However, diode laser when used in conjunction with sodium hypochlorite as a disinfecting modality was found to be the most effective for the eradication of Enterococcus faecalis from infected root canals.

scholarly journals Effect of Hydrogen Peroxide on the Antibacterial Substantivity of Chlorhexidine

2010 ◽  
Vol 2010 ◽  
pp. 1-4 ◽  
Author(s):  
Shahriar Shahriari ◽  
Zahed Mohammadi ◽  
Mohammadi Mehdi Mokhtari ◽  
Rasoul Yousefi
Keyword(s):  
Hydrogen Peroxide ◽  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Time Lapse ◽  
Positive Control ◽  
Negative Control ◽  
The Other ◽  
Time Period ◽  
Colony Forming Units

The purpose of thisin vitrostudy was to assess the effect of hydrogen peroxide on the antibacterial substantivity of chlorhexidine (CHX). Seventy-five dentine tubes prepared from human maxillary central and lateral incisor teeth were used. After contamination withEnterococcus faecalisfor 14 days, the specimens were divided into five groups as follows: CHX, H2O2, CHX + H2O2, infected dentine tubes (positive control), and sterile dentine tubes (negative control). Dentine chips were collected with round burs into tryptic soy broth, and after culturing, the number of colony-forming units (CFU) was counted. The number of CFU was minimum in the first cultures in all experimental groups, and the results obtained were significantly different from each other at any time period (). At the first culture, the number of CFU in the CHX + H2O2group was lower than other two groups. At the other experimental periods, the CHX group showed the most effective antibacterial action (). Hydrogen peroxide group showed the worst result at all periods. In each group, the number of CFU increased significantly by time lapse (). In conclusion, H2O2had no additive effect on the residual antibacterial activity of CHX.

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