Quantitative Approaches to Delineate Passive Transport Mechanisms in Cell Culture Monolayers

1999 ◽  
Author(s):  
Norman Ho
Keyword(s):  
Cell Culture ◽  
Transport Mechanisms ◽  
Passive Transport ◽  
Quantitative Approaches

Absorption from gastrointestinal tract of the rat after x-irradiation

1961 ◽  
Vol 201 (6) ◽  
pp. 1013-1016 ◽  
Author(s):  
Maurice F. Sullivan
Keyword(s):  
Nitrogen Mustard ◽  
Direct Action ◽  
Glucose Absorption ◽  
The Other ◽  
Transport Mechanisms ◽  
Passive Transport ◽  
Sugar Absorption ◽  
In Situ Perfusion ◽  
X Irradiation

An in situ perfusion method was used to measure glucose absorption from the x-irradiated or nitrogen-mustard-treated rat. Inhibition was greatest 3 days after treatment and recovery had begun by the 6th day. The effect on absorption was not appreciably different after 900 r than after 1,500 r, although histologic damage was much greater after the higher dose. Pretreatment with cysteine or AET prevented the decreased absorption after 900 r but did not protect against 1,500 r nor against the effect of HN2. The absorption of d(+)-xylose was also decreased 3 days after irradiation of the intestine. Thus, both active and passive transport mechanisms involved in sugar absorption were decreased by irradiation of the intestine. The absorption of fat, on the other hand, was not decreased as a result of direct action by x-radiation on the intestinal epithelium, since oleic acid absorption was unaffected by exposure of the intestine to radiation.

Contribution of Passive Transport Mechanisms to the Intestinal Absorption of β-Lactam Antibiotics

1990 ◽  
Vol 42 (5) ◽  
pp. 314-318 ◽  
Author(s):  
Mitsuru Sugawara ◽  
Hiroshi Saitoh ◽  
Ken Iseki ◽  
Katsumi Miyazaki ◽  
Takaichi Arita
Keyword(s):  
Intestinal Absorption ◽  
Transport Mechanisms ◽  
Passive Transport

Transepithelial transport mechanisms of 7,8-dihydroxyflavone, a small molecular TrkB receptor agonist, in human intestinal Caco-2 cells

2019 ◽  
Vol 10 (8) ◽  
pp. 5215-5227 ◽  
Author(s):  
Yufeng Chen ◽  
Fan Xue ◽  
Guobin Xia ◽  
Zhenlei Zhao ◽  
Chun Chen ◽  
...  
Keyword(s):  
Receptor Agonist ◽  
Transepithelial Transport ◽  
Efflux Transporters ◽  
Transport Mechanisms ◽  
Passive Transport ◽  
Cell Monolayers ◽  
Trkb Receptor

7,8-DHF was transported across Caco-2 cell monolayers by passive transport with carrier-mediated influx and efflux transporters.

Vitamin absorption in thein vivointestine of normal and infected (Hymenolepis diminuta: Cestoda) rats

1979 ◽  
Vol 53 (3) ◽  
pp. 213-222 ◽  
Author(s):  
D. F. Mettrick ◽  
D. J. Jackson
Keyword(s):  
Folic Acid ◽  
Regional Differences ◽  
Hymenolepis Diminuta ◽  
Water Fluxes ◽  
Transport Mechanisms ◽  
Passive Transport ◽  
Intestinal Uptake ◽  
Vitamin Uptake ◽  
Mucosal Uptake

ABSTRACTUptake and serosal transfer of the vitamins thiamine, riboflavin and folic acid have been studiedin vivoin normal and parasitized rats infected withHymenolepis diminuta(Cestoda).Regional differences in intestinal uptake of all three vitamins in both uninfected and parasitized animals were not statistically significant.In the parasitized intestine mucosal uptake and serosal transfer of thiamine were significantly inhibited, with increased mucosal accumulation of the vitamin as luminal thiamine concentration increased.Apparent increased riboflavin mucosal uptake in parasitized animals, was not matched by the reduced serosal transfer, suggesting adsorption of the vitamin in the unstirred aqueous layers.Mucosal uptake of folic acid increased in the parasitized gut; serosal transfer and mucosal accumulation were not affected.These results, indicating vitamin malabsorption associated with infection byH. diminuta, are consistent with the parasite inhibiting mucosal passive transport mechanisms. This conclusion is supported by the changes in net water fluxes associated with vitamin uptake in the parasitized intestine.

scholarly journals Evaluation of the potential of (pelargonium roseum) scented geranium plant to uptake and accumulate lead

2019 ◽  
pp. 139-145
Author(s):  
Majid Mahdiyeh ◽  
Mohammad Reza Sangi ◽  
Malek Soleimani ◽  
Abolfazle Tagavi
Keyword(s):  
Day Treatment ◽  
Culture Solution ◽  
Transport Mechanisms ◽  
Passive Transport ◽  
Lead Uptake ◽  
Major Objective ◽  
Soil System ◽  
Linear Pattern ◽  
High Concentrations ◽  
Pelargonium Roseum

The major objective of this investigation was to evaluate the potential of scentedgeranium plant, Pelargonium roseum to uptake and accumulate lead under greenhouseconditions. Plants were grown in an artificial soil system and exposed to a range of leadconcentrations (0-2500mg.L- 1 Pb (NO3)2) in over a 14 day treatment period. The resultsshowed that the uptake and accumulation of lead in shoot and root followed a similartrend. The highest amount of lead accumulation was detected at 2500mg.L- 1 lead nitratein the culture solution that was 86566.68±7864 mg.kg-' DW for roots and4416.04±502.27 mg.kg-' DW for shoots. The linear pattern of uptake suggests theinvolvement of both active and passive transport mechanisms for lead uptake. Finally,because the high concentrations of lead found in shoots of scented geranium plants hasfar exceeded 0.1 % DW, Pelargonium roseum may be a new hyperaccumulator speciesfor this metal and be used for phytoremediation industry.

Physical Properties of Isolated Perfused Renal Tubular Basement Membranes

1971 ◽  
Vol 29 ◽  
pp. 390-391
Author(s):  
Jared Grantham ◽  
Larry Welling
Keyword(s):  
Basement Membrane ◽  
Basement Membranes ◽  
Transport Mechanisms ◽  
Permeability Characteristics ◽  
Peritubular Capillaries ◽  
Strategic Location ◽  
Tubular Lumen ◽  
Glomerular Filtrate ◽  
Urine Formation ◽  
Renal Tubular

In the course of urine formation in mammalian kidneys over 90% of the glomerular filtrate moves from the tubular lumen into the peritubular capillaries by both active and passive transport mechanisms. In all of the morphologically distinct segments of the renal tubule, e.g. proximal tubule, loop of Henle and distal nephron, the tubular absorbate passes through a basement membrane which rests against the basilar surface of the epithelial cells. The basement membrane is in a strategic location to affect the geometry of the tubules and to influence the movement of tubular absorbate into the renal interstitium. In the present studies we have determined directly some of the mechanical and permeability characteristics of tubular basement membranes.

A T.E.M. study of mouse mammary epithelial cell secretory differentiation cultured on collagen and Matrigel

1991 ◽  
Vol 49 ◽  
pp. 188-189
Author(s):  
W.N. Bentham ◽  
V. Rocha
Keyword(s):  
Cell Culture ◽  
Mammary Epithelial ◽  
Secretory Process ◽  
Cell Culture System ◽  
Protein Maturation ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Mouse Mammary Epithelial Cell ◽  
Secretory Differentiation

It has been an interest of our lab to develop a mammary epethelial cell culture system that faithfully duplicates the in vivo condition of the lactating gland. Since the introduction of collagen as a matrix on which cells are cultivated other E.C.M. type matrices have been made available and are used in many cell culture techniques. We have previously demonstrated that cells cultured on collagen and Matrigel do not differentiate as they do in vivo. It seems that these cultures often produce cells that show a disruption in the secretory process. The appearance of large ribosomal studded vesicles, that specifically label with antibody to casein, suggest an interruption of both protein maturation and secretion at the E.R. to golgi transition. In this report we have examined cultures on collagen and Matrigel at relative high and low seeding densities and compared them to cells from the in vivo condition.

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