Performance of a biotrickling filter for anaerobic utilization of gas-phase methanol coupled to thiosulphate reduction and resource recovery through volatile fatty acids production

Summary A method of acetate determination by gas phase chromatography using a porous polymer stationary phase is reported that is suitable for use with aqueous and plasma samples. It is linear up to 100 mmol/l and has a coefficient of variation of less than 4 % for acetate values of greater than 1 mmol/l. The recovery of acetate from plasma is 92 % and sample retention time is 3 minutes. The reference range of plasma acetate in a group of 40 apparently healthy subjects was from less than 0·1 to 0·35 mmol/l. The frequently encountered problem of adsorption and ghosting of volatile fatty acids is overcome without the addition of formic acid vapour to the carrier gas.

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Online headspace chromatographic method for measuring VFA in biogas reactor

Water Science & Technology ◽

10.2166/wst.2005.0555 ◽

2005 ◽

Vol 52 (1-2) ◽

pp. 473-478 ◽

Cited By ~ 17

Author(s):

K. Boe ◽

D.J. Batstone ◽

I. Angelidaki

Keyword(s):

Fatty Acids ◽

Organic Acid ◽

Gas Phase ◽

Volatile Fatty Acids ◽

Chromatographic Method ◽

Negative Impact ◽

Automated System ◽

Liquid Volume ◽

Strong Impact ◽

Short Period

A headspace chromatographic method has been applied to measure volatile fatty acids in anaerobic digesters using gas phase extraction at pH less than 2 and temperature higher than 65 °C. The concentration of volatile fatty acids in liquid and gas phase can be correlated in the form of Henry's coefficient. Analysis of different factors in the batch indicated that pH, temperature, and salt addition had a strong impact on apparent solubility, while liquid/gas volume ratio and organic acid concentration had little impact. Larger liquid volume had a positive impact on extraction efficiency, while increased gas headspace had a negative impact, indicating that the system was total mass-limited. The best conditions were at pH < 2.0, and temperatures above 75 °C. The advantage of an automated gas-extraction system for organic acid analysis, compared to an automated liquid system is that it contains no filter element, which is susceptible to fouling in manure samples or samples with high solid content. An automated system which included a valerate pulse to the parent reactor was implemented, and operated for a short period.

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Treatment of odorous volatile fatty acids using a biotrickling filter

Bioresource Technology ◽

10.1016/j.biortech.2006.12.032 ◽

2008 ◽

Vol 99 (3) ◽

pp. 589-595 ◽

Cited By ~ 20

Author(s):

Y.F. Tsang ◽

H. Chua ◽

S.N. Sin ◽

S.Y. Chan

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Biotrickling Filter

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Unravelling a microbial synergy to boost caproate production via carboxylates chain elongation with ethanol

HKIE Transactions ◽

10.33430/v26n2thie-2018-0041 ◽

2019 ◽

Vol 26 (2) ◽

pp. 63-71

Author(s):

Ling Leng ◽

Ying Wang ◽

Peixian Yang ◽

Takashi Narihiro ◽

Masaru Konishi Nobu ◽

...

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Microbial Consortia ◽

Chain Elongation ◽

Desulfovibrio Vulgaris ◽

Rrna Gene ◽

Selective Enrichment ◽

Microbial Network ◽

Cost Efficient ◽

Rrna Gene Sequencing

Chain elongation of volatile fatty acids for medium chain fatty acids production (e.g. caproate) is an attractive approach to treat wastewater anaerobically and recover resource simultaneously. Undefined microbial consortia can be tailored to achieve chain elongation process with selective enrichment from anaerobic digestion sludge, which has advantages over pure culture approach for cost-efficient application. Whilst the metabolic pathway of the dominant caproate producer, Clostridium kluyveri, has been annotated, the role of other coexisting abundant microbiomes remained unclear. To this end, an ethanol-acetate fermentation inoculated with fresh digestion sludge at optimal conditions was conducted. Also, physiological study, thermodynamics and 16 S rRNA gene sequencing to elucidate the biological process by linking the system performance and dominant microbiomes were integrated. Results revealed a possible synergistic network in which C. kluyveri and three co-dominant species, Desulfovibrio vulgaris, Fusobacterium varium and Acetoanaerobium sticklandii coexisted. D. vulgaris and A. sticklandii (F. varium) were likely to boost the carboxylates chain elongation by stimulating ethanol oxidation and butyrate production through a syntrophic partnership with hydrogen (H2) serving as an electron messenger. This study unveils a synergistic microbial network to boost caproate production in mixed culture carboxylates chain elongation.

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МЕТАБОЛИТЫ ПРОБИОТИЧЕСКИХ БАКТЕРИЙ ОТВЕЧАЮТ ЗА ЭФФЕКТИВНОСТЬ ДЕЙСТВИЯ ПРОБИОТИКА "ПРОФОРТ®"

10.33845/0033-3239-2019-68-9-10-45-47 ◽

2019 ◽

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Broiler Chicks ◽

Bacillus Sp

МЕЛИКИДИ В.Х., ТЮРИНА Д.Г., СЕЛИВАНОВ Д.Г., НОВИКОВА Н.И. ООО «БИОТРОФ», Санкт-Петербург Аннотация: Приведены данные исследования методом газожидкостной хроматомасс-спектрометрии метаболитов, синтезируемых пробиотическими бактериями, входящими в состав кормовой добавки «Профорт®». Проведен опыт в условиях интенсивного промышленного птицеводства по применению кормовой добавки «Профорт®» (50 тыс. голов бройлеров в группе). Среди метаболитов пробиотических штаммов Enterococcus sp. и Bacillus sp. обнаружены такие полезные вещества, как молочная кислота, уксусная, пропионовая и другие короткоцепочечные (летучие) жирные кислоты, активные пептиды. Результаты зоотехнического опыта показали, что при скармливании бройлерам пробиотика «Профорт®» (500 г/т) живая масса при убое в 40 дней была выше контроля на 6,9%, конверсия корма улучшилась на 3,0%, а европейский индекс продуктивности бройлеров - на 5,69%. Ключевые слова: ПРОБИОТИКИ, МЕТАБОЛИТЫ, ЛЕТУЧИЕ ЖИРНЫЕ КИСЛОТЫ, ЦЫПЛЯТА-БРОЙЛЕРЫ,ПРОДУКТИВНОСТЬ, PROBIOTICS, METABOLITES, VOLATILE FATTY ACIDS, BROILER CHICKS,PRODUCTIVITY

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