The Development of In Vitro Models for the Blood–Brain and Blood–CSF Barriers

2000 ◽  
Author(s):  
Dorothea Korte ◽  
Helmut Franke ◽  
Joachim Wegener ◽  
Matthias Haselbach ◽  
Hans-Joachim Galla ◽  
...  
Keyword(s):  
In Vitro Models ◽  
Blood Brain

scholarly journals Biochemical‐ and Biophysical‐Induced Barriergenesis in the Blood–Brain Barrier: A Review of Barriergenic Factors for Use in In Vitro Models

2021 ◽  
Vol 1 (5) ◽  
pp. 2170051
Author(s):  
Christina L. Schofield ◽  
Aleixandre Rodrigo-Navarro ◽  
Matthew J. Dalby ◽  
Tom Van Agtmael ◽  
Manuel Salmeron-Sanchez
Keyword(s):  
Blood Brain Barrier ◽  
In Vitro Models ◽  
Brain Barrier ◽  
Blood Brain

Understanding the Physiology of the Blood-Brain Barrier: In Vitro Models

Physiology ◽  
1998 ◽  
Vol 13 (6) ◽  
pp. 287-293 ◽  
Author(s):  
Gerald A. Grant ◽  
N. Joan Abbott ◽  
Damir Janigro
Keyword(s):  
Central Nervous System ◽  
Tissue Culture ◽  
Nervous System ◽  
Blood Brain Barrier ◽  
In Vitro Models ◽  
Brain Barrier ◽  
Blood Brain ◽  
Brain Tissue Culture ◽  
The Brain

Endothelial cells exposed to inductive central nervous system factors differentiate into a blood-brain barrier phenotype. The blood-brain barrier frequently obstructs the passage of chemotherapeutics into the brain. Tissue culture systems have been developed to reproduce key properties of the intact blood-brain barrier and to allow for testing of mechanisms of transendothelial drug permeation.

Putative proteins involved in manganese transport across the blood-brain barr 1ier

2007 ◽  
Vol 26 (4) ◽  
pp. 295-302 ◽  
Author(s):  
Vanessa A Fitsanakis ◽  
Greg Piccola ◽  
Ana Paula Marreilha dos Santos ◽  
Judy L Aschner ◽  
Michael Aschner
Keyword(s):  
In Vitro Models ◽  
Complex Nature ◽  
Divalent Metal Transporter 1 ◽  
Divalent Metal Transporter ◽  
Culture Techniques ◽  
Metal Transporter ◽  
Blood Brain ◽  
Essential Nutrient ◽  
Manganese Transport

Manganese (Mn) is an essential nutrient required for proper growth and maintenance of numerous biological systems. At high levels it is known to be neurotoxic. While focused research concerning the transport of Mn across the blood-brain barrier (BBB) is on-going, the exact identity of the transporter(s) responsible is still debated. The transferrin receptor (TfR) and the divalent metal transporter-1 (DMT-1) have long been thought to play a role in brain Mn deposition. However, evidence suggests that Mn may also be transported by other proteins. One model system of the BBB, rat brain endothelial (RBE4) cells, are known to express many proteins suspected to be involved in metal transport. This review will discuss the biological importance of Mn, and then briefly describe several proteins that may be involved in transport of this metal across the BBB. The latter section will examine the potential usefulness of RBE4 cells in characterizing various aspects of Mn transport, and basic culture techniques involved in working with these cells. It is hoped that ideas put forth in this article will stimulate further investigations into the complex nature of Mn transport, and address the importance as well as the limitation of in vitro models in answering these questions. Human & Experimental Toxicology (2007) 26, 295-302

Claudin-5 Expression in In Vitro Models of the Blood–Brain Barrier

2011 ◽  
pp. 347-354 ◽  
Author(s):  
Itzik Cooper ◽  
Katayun Cohen-Kashi-Malina ◽  
Vivian I. Teichberg
Keyword(s):  
Blood Brain Barrier ◽  
In Vitro Models ◽  
Brain Barrier ◽  
Blood Brain

Transport Studies Using Blood-Brain Barrier In Vitro Models: A Critical Review and Guidelines

2020 ◽  
Author(s):  
Ana R. Santa-Maria ◽  
Marjolein Heymans ◽  
Fruzsina R. Walter ◽  
Maxime Culot ◽  
Fabien Gosselet ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Critical Review ◽  
In Vitro Models ◽  
Brain Barrier ◽  
Transport Studies ◽  
Blood Brain

scholarly journals Mechanisms of Glutamate Efflux at the Blood—Brain Barrier: Involvement of Glial Cells

2011 ◽  
Vol 32 (1) ◽  
pp. 177-189 ◽  
Author(s):  
Katayun Cohen-Kashi-Malina ◽  
Itzik Cooper ◽  
Vivian I Teichberg
Keyword(s):  
Endothelial Cells ◽  
Blood Brain Barrier ◽  
Glial Cells ◽  
Excitatory Amino Acid ◽  
Active Role ◽  
In Vitro Models ◽  
Brain Barrier ◽  
Amino Acid Transporters ◽  
Blood Brain

At high concentrations, glutamate (Glu) exerts potent neurotoxic properties, leading to irreversible brain damages found in numerous neurological disorders. The accepted notion that Glu homeostasis in brain interstitial fluid is maintained primarily through the activity of Glu transporters present on glial cells does not take into account the possible contribution of endothelial cells constituting the blood-brain barrier (BBB) to this process. Here, we present evidence for the presence of the Glu transporters, excitatory amino-acid transporters (EAATs) 1 to 3, in porcine brain endothelial cells (PBECs) and show their participation in Glu uptake into PBECs. Moreover, transport of Glu across three in vitro models of the BBB is investigated for the first time, and evidence for Glu transport across the BBB in both directions is presented. Our results provide evidence that the BBB can function in the efflux mode to selectively remove Glu, via specific transporters, from the abluminal side (brain) into the luminal compartment (blood). Furthermore, we found that glial cells lining the BBB have an active role in the efflux process by taking up Glu and releasing it, through hemichannels, anion channels, and possibly the reversal of its EAATs, in close proximity to ECs, which in turn take up Glu and release it to the blood.

New approaches to in vitro models of blood–brain barrier drug transport

2003 ◽  
Vol 8 (20) ◽  
pp. 944-954 ◽  
Author(s):  
Tetsuya Terasaki ◽  
Sumio Ohtsuki ◽  
Satoko Hori ◽  
Hitomi Takanaga ◽  
Emi Nakashima ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Drug Transport ◽  
In Vitro Models ◽  
Brain Barrier ◽  
New Approaches ◽  
Blood Brain
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