Transgenic Rice for Disease Resistance

2004 ◽  
pp. 171-190
Plant Disease ◽  
2021 ◽  
Author(s):  
Suresh Pokhrel ◽  
Sathish K Ponniah ◽  
Yulin Jia ◽  
Oliver Yu ◽  
Muthusamy Manoharan

The isoflavones are a group of plant secondary metabolites primarily synthesized in legumes and are known for their role in improving human health and plant disease resistance. The isoflavones, especially genistein, act as precursors for the production of phytoalexins, which may induce broad-spectrum disease resistance in plants. In the present study, we screened transgenic rice lines expressing the isoflavone synthase (GmIFS1) gene from soybean for rice blast (Magnaporthe oryzae) resistance. Two homozygous transgenic lines (I2 and I10), based on single copy gene integration, were identified. The expression of GmIFS1 in transgenic lines was confirmed by qRT-PCR. Genistein was detected in the transgenic lines using LC-MS/MS. Subsequently, the transgenic lines were evaluated against the rice blast pathogen, isolate YJ54 (race IB-54). The results indicated that more than 60% of the plants in both the lines (I2 and I10) showed resistance against the blast pathogen. The progenies of one of the resistant transgenic lines (I10) also showed more than 65% resistance against rice blast. The resistance of these transgenic lines against rice blast may be attributed to the synthesis of isoflavone (genistein) in rice.


2007 ◽  
Vol 48 (9) ◽  
pp. 1263-1274 ◽  
Author(s):  
A. Yara ◽  
T. Yaeno ◽  
M. Hasegawa ◽  
H. Seto ◽  
J.-L. Montillet ◽  
...  

2006 ◽  
Vol 5 (11) ◽  
pp. 805-811 ◽  
Author(s):  
Heng-xiu YU ◽  
Qiao-quan LIU ◽  
Ling WANG ◽  
Zhi-peng ZHAO ◽  
Li XU ◽  
...  

2018 ◽  
Vol 19 (8) ◽  
pp. 2025-2035 ◽  
Author(s):  
Jun Wang ◽  
Xuan Zeng ◽  
Dongsheng Tian ◽  
Xiaobei Yang ◽  
Lanlan Wang ◽  
...  

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e78348 ◽  
Author(s):  
Xian Yong Zhang ◽  
Zhuan Hua Nie ◽  
Wen Juan Wang ◽  
David W. M. Leung ◽  
Da Gao Xu ◽  
...  

PLoS ONE ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. e0175734 ◽  
Author(s):  
Zhenzhen Wang ◽  
Qiang Han ◽  
Qian Zi ◽  
Shun Lv ◽  
Dewen Qiu ◽  
...  

2008 ◽  
Vol 9 (4) ◽  
pp. 525-529 ◽  
Author(s):  
YOSHIMITSU TAKAKURA ◽  
FANG‐SIK CHE ◽  
YUJI ISHIDA ◽  
FUMIKI TSUTSUMI ◽  
KEN‐ICHI KUROTANI ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document