scholarly journals Quantitative Assessment of Mouse Mammary Gland Morphology Using Automated Digital Image Processing and TEB Detection

Endocrinology ◽  
2016 ◽  
Vol 157 (4) ◽  
pp. 1709-1716 ◽  
Author(s):  
Silvia Blacher ◽  
Céline Gérard ◽  
Anne Gallez ◽  
Jean-Michel Foidart ◽  
Agnès Noël ◽  
...  

Abstract The assessment of rodent mammary gland morphology is largely used to study the molecular mechanisms driving breast development and to analyze the impact of various endocrine disruptors with putative pathological implications. In this work, we propose a methodology relying on fully automated digital image analysis methods including image processing and quantification of the whole ductal tree and of the terminal end buds as well. It allows to accurately and objectively measure both growth parameters and fine morphological glandular structures. Mammary gland elongation was characterized by 2 parameters: the length and the epithelial area of the ductal tree. Ductal tree fine structures were characterized by: 1) branch end-point density, 2) branching density, and 3) branch length distribution. The proposed methodology was compared with quantification methods classically used in the literature. This procedure can be transposed to several software and thus largely used by scientists studying rodent mammary gland morphology.

Biometrics ◽  
2017 ◽  
pp. 382-402
Author(s):  
Petre Anghelescu

In this paper are presented solutions to develop algorithms for digital image processing focusing particularly on edge detection. Edge detection is one of the most important phases used in computer vision and image processing applications and also in human image understanding. In this chapter, implementation of classical edge detection algorithms it is presented and also implementation of algorithms based on the theory of Cellular Automata (CA). This work is totally related to the idea of understanding the impact of the inherently local information processing of CA on their ability to perform a managed computation at the global level. If a suitable encoding of a digital image is used, in some cases, it is possible to achieve better results in comparison with the solutions obtained by means of conventional approaches. The software application which is able to process images in order to detect edges using both conventional algorithms and CA based ones is written in C# programming language and experimental results are presented for images with different sizes and backgrounds.


2018 ◽  
Vol 53 (1) ◽  
pp. 45-63 ◽  
Author(s):  
Ewald Fauster ◽  
David C Berg ◽  
Dilmurat Abliz ◽  
Harald Grössing ◽  
Dieter Meiners ◽  
...  

Textile permeability is one of the dictating factors in the fabrication of fibre-reinforced polymer composites. However, reproducibility of experimental in-plane permeability characterization is still a challenging task due to the lack of standardized test and evaluation procedures. The paper at hand addresses two major sources for discrepancies when characterizing in-plane permeability through optical observation of radial flow experiments: digital image processing and data evaluation algorithms. A digital image processing strategy is presented, which robustly handles varying lightning conditions, optical properties of the materials under test and image occlusions caused by mechanical elements of the test setup. The strategy is of universal validity and independent of the choice of reinforcing material and impregnating fluid. An experimental analysis compares two approaches for fitting elliptic geometry models to data points detected along the fluid flow front. The study reveals the impact of the fitting strategy on the resulting permeability data and the benefit of forcing the ellipse centre to that of the injection opening. The computation algorithm of Chan and Hwang, widely used for calculating in-plane permeability values from experimental data, is critically discussed. A correction of the algorithm is proposed which avoids a violation of isotropic data characteristics while adding robustness to the data reduction. An experimental analysis compares anisotropic in-plane permeability values obtained with different evaluation algorithms. The study highlights the impact of the computational algorithm on the permeability data and reveals discrepancies of up to 6%, which is considerable compared to the scatter typically reported for in-plane permeability data.


Author(s):  
Petre Anghelescu

In this paper are presented solutions to develop algorithms for digital image processing focusing particularly on edge detection. Edge detection is one of the most important phases used in computer vision and image processing applications and also in human image understanding. In this chapter, implementation of classical edge detection algorithms it is presented and also implementation of algorithms based on the theory of Cellular Automata (CA). This work is totally related to the idea of understanding the impact of the inherently local information processing of CA on their ability to perform a managed computation at the global level. If a suitable encoding of a digital image is used, in some cases, it is possible to achieve better results in comparison with the solutions obtained by means of conventional approaches. The software application which is able to process images in order to detect edges using both conventional algorithms and CA based ones is written in C# programming language and experimental results are presented for images with different sizes and backgrounds.


2014 ◽  
Vol 626 ◽  
pp. 258-263
Author(s):  
Ling Ling Hu ◽  
Li Wen Xiong ◽  
T.X. Yu

A numerical model of the aluminium foam with voronoi cells is built and uni-directionally crushed with various velocities from 1m/s to 110m/s. It is shown that the foam deforms homogeneously within the whole specimen and the stress in the foam increases gradually with the strain without an obvious plateau stage under the low-velocity compression, while the deformation is concentrated within a zone near the impact end and an obvious plateau stage can be found in the stress-strain curves of the foams under the high-velocity crushing. By analyzing the distribution of the density within the foams using the digital image processing technology, the densification strain of the foams under dynamic crushing can be determined. Then combining the foam’s stress-strain curve under the low-velocity compression, the dynamic plateau stress of the foams can be predicted. It is shown that both the densification strain and the plateau stress of the foams under the high-velocity crushing predicted by employing the digital image process technology are in good agreement with the numerical simulations. The results show that both the plateau stress and the densification strain of the foams increase with the impact velocity, which is essentially caused by the localization of the foam’s deformation under dynamic crushing.


2020 ◽  
Vol 44 (2) ◽  
pp. 171-178
Author(s):  
P. Arul Jose ◽  
T. Sasikumar ◽  
P. Arun Bose ◽  
N. Prabhu

In this paper, fibre epoxy composite specimens are prepared by hand lay-up method with stacking sequences (04/±452)s. Five specimens are prepared with the same dimensions and named as S1, S2, S3, S4, and S5. These specimens are tested for impact analysis using a drop-weight apparatus (DWA-Ceast9350) at energy levels of 25, 30, and 35 J, followed by tensile testing using a universal testing machine (Dak9103). The damaged areas of the impacted specimens are examined by digital image processing, numerically simulated ABAQUS software, and scanning electron microscopy, followed by tensile strength results. The results of the experiment reveal that when the impact energy increases, the damage area increases, the tensile strength decreases, and the cracks formed during impact are shown in scanning electron microscope imaging. The homogeneity and isotropy of the composite are identified by the Feret ratio and circular shape factor.


Author(s):  
R. C. Gonzalez

Interest in digital image processing techniques dates back to the early 1920's, when digitized pictures of world news events were first transmitted by submarine cable between New York and London. Applications of digital image processing concepts, however, did not become widespread until the middle 1960's, when third-generation digital computers began to offer the speed and storage capabilities required for practical implementation of image processing algorithms. Since then, this area has experienced vigorous growth, having been a subject of interdisciplinary research in fields ranging from engineering and computer science to biology, chemistry, and medicine.


Author(s):  
L. Montoto ◽  
M. Montoto ◽  
A. Bel-Lan

INTRODUCTION.- The physical properties of rock masses are greatly influenced by their internal discontinuities, like pores and fissures. So, these need to be measured as a basis for interpretation. To avoid the basic difficulties of measurement under optical microscopy and analogic image systems, the authors use S.E.M. and multiband digital image processing. In S.E.M., analog signal processing has been used to further image enhancement (1), but automatic information extraction can be achieved by simple digital processing of S.E.M. images (2). The use of multiband image would overcome difficulties such as artifacts introduced by the relative positions of sample and detector or the typicals encountered in optical microscopy.DIGITAL IMAGE PROCESSING.- The studied rock specimens were in the form of flat deformation-free surfaces observed under a Phillips SEM model 500. The SEM detector output signal was recorded in picture form in b&w negatives and digitized using a Perkin Elmer 1010 MP flat microdensitometer.


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