Polymorphic Forms of Expressed Bovine Interferon-τ Genes: Relative Transcript Abundance during Early Placental Development, Promoter Sequences of Genes and Biological Activity of Protein Products*

Two rice accessions, Capataz and Beirao, contrasting for cadmium (Cd) tolerance and root retention, were exposed to a broad range of Cd concentrations (0.01, 0.1, and 1 μM) and analyzed for their potential capacity to chelate, compartmentalize, and translocate Cd to gain information about the relative contribution of these processes in determining the different pathways of Cd distribution along the plants. In Capataz, Cd root retention increased with the external Cd concentration, while in Beirao it resulted independent of Cd availability and significantly higher than in Capataz at the lowest Cd concentrations analyzed. Analysis of thiol accumulation in the roots revealed that the different amounts of these compounds in Capataz and Beirao, as well as the expression levels of genes involved in phytochelatin biosynthesis and direct Cd sequestration into the vacuoles of the root cells, were not related to the capacity of the accessions to trap the metal into the roots. Interestingly, the relative transcript abundance of OsHMA2, a gene controlling root-to-shoot Cd/Zn translocation, was not influenced by Cd exposure in Capataz and progressively increased in Beirao with the external Cd concentration, suggesting that activity of the OsHMA2 transporter may differentially limit root-to-shoot Cd/Zn translocation in Capataz and Beirao.

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Uterine crowding in the sow affects litter sex ratio, placental development and embryonic myogenin expression in early gestation

Reproduction Fertility and Development ◽

10.1071/rd07200 ◽

2008 ◽

Vol 20 (4) ◽

pp. 497 ◽

Cited By ~ 11

Author(s):

W.-Y. Tse ◽

S. C. Town ◽

G. K. Murdoch ◽

S. Novak ◽

M. K. Dyck ◽

...

Keyword(s):

Sex Ratio ◽

Muscle Fibre ◽

Transcript Abundance ◽

Early Embryo ◽

Myoblast Differentiation ◽

Placental Development ◽

Myogenic Regulatory Factor ◽

Fibre Development ◽

Differentiation And Proliferation ◽

Fetal Muscle

Uterine crowding in the pig results in intrauterine growth restriction (IUGR), and permanently affects fetal muscle fibre development, representing production losses for the commercial pig herd. The present study sought to understand how different levels of uterine crowding in sows affects muscle fibre development in the early embryo at the time of muscle fibre differentiation and proliferation. Sows either underwent surgical, unilateral oviduct ligation (LIG; n = 10) to reduce the number of embryos in the uterus, or remained as intact, relatively-crowded controls (CTR; n = 10). Embryos and placentae were collected at Day 30 of gestation, and myogenic regulatory factor (MRF) transcript abundance was determined using real-time PCR for both myogenin (MYOG) and myoblast differentiation 1 (MYOD1). Unilateral tubal ligation resulted in lower numbers of embryos in utero, higher placental weights and a higher male : female sex ratio (P < 0.05). Relative MYOD1 expression was not different, but MYOG expression was higher (P < 0.05) in the LIG group embryos; predominantly due to effects on the male embryos. Relatively modest uterine crowding therefore affects MRF expression, even at very early stages of embryonic development, and could contribute to reported differences in fetal muscle fibre development, birthweight and thus post-natal growth performance in swine.

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Detection of abscisic acid and relative transcript abundance in Syntrichia caninervis Mitt.

Journal of Bryology ◽

10.1080/03736687.2021.1989927 ◽

2021 ◽

pp. 1-8

Author(s):

Yigong Zhang ◽

Ayibaiheremu Mutailifu ◽

Yi Zhang ◽

Honglan Yang ◽

Daoyuan Zhang

Keyword(s):

Abscisic Acid ◽

Transcript Abundance ◽

Syntrichia Caninervis ◽

Relative Transcript Abundance

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Using the histone H2a transcript as an endogenous standard to study relative transcript abundance during bovine early development

Molecular Reproduction and Development ◽

10.1002/mrd.20665 ◽

2007 ◽

Vol 74 (6) ◽

pp. 703-715 ◽

Cited By ~ 25

Author(s):

Christian Vigneault ◽

Isabelle Gilbert ◽

Marc-André Sirard ◽

Claude Robert

Keyword(s):

Early Development ◽

Transcript Abundance ◽

Histone H2a ◽

Relative Transcript Abundance

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Synthesis, Crystal Structure, and Biological Activity of Ethyl 4-Methyl-2,2-dioxo-1H-2λ6,1-benzothiazine-3-carboxylate Polymorphic Forms

Scientia Pharmaceutica ◽

10.3390/scipharm86020021 ◽

2018 ◽

Vol 86 (2) ◽

pp. 21 ◽

Cited By ~ 4

Author(s):

Igor Ukrainets ◽

Anna Burian ◽

Vyacheslav Baumer ◽

Svitlana Shishkina ◽

Lyudmila Sidorenko ◽

...

Keyword(s):

Crystal Structure ◽

Biological Activity ◽

Polymorphic Forms

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Maternal nutrition affects the composition of follicular fluid and transcript content in gilt oocytes

Veterinární Medicína ◽

10.17221/1573-vetmed ◽

2011 ◽

Vol 56 (No. 4) ◽

pp. 156-167 ◽

Cited By ~ 14

Author(s):

E. Warzych ◽

A. Cieslak ◽

P. Pawlak ◽

N. Renska ◽

E. Pers-Kamczyc ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Fatty Acid Profile ◽

Follicular Fluid ◽

Diet Composition ◽

Transcript Abundance ◽

Marker Genes ◽

Fluid Composition ◽

Experimental Diet ◽

Relative Transcript Abundance

Metabolomics focused on reproduction have been the subject of special interest in the past decade. Metabolite profiling provides detailed information on the status of follicular fluid and follicular cells which accompany the growing oocyte. Although nutrients present in the diet reach oocytes via the follicular fluid, it is not evident whether oocyte/embryo quality can be predicted based on the follicular fluid composition. Since this phenomenon has not yet been investigated in the pig, the aim of the present study was to investigate associations between diet composition and (1) selected markers related to developmental potential of oocytes (brilliant cresyl blue – BCB test, relative transcript abundance of EEF1A1 and ATP5A1 marker genes) and (2) fatty acid profile in the follicular fluid. Gilts were fed control and experimental diets which differed in composition and fatty acid profiles. The experimental diet used in this study comprised mainly locally grown grains (barley and rye) traditionally used for fattening pigs in Poland. Cumulus-oocyte complexes were aspirated from individual pairs of ovaries after animal slaughter, evaluated morphologically and subjected to the BCB test. Relative transcript abundance for the two marker genes was monitored by real-time PCR in oocytes of both categories (BCB+ and BCB–). Fatty acid profile in follicular fluid was analyzed by gas chromatography. We show that the experimental diet rich in n-3 fatty acid significantly influences fatty acid composition of the follicular fluid. The fatty acid profile of the follicular fluid of gilts fed the experimental diet differed from that of the control females. The content of saturated fatty acids was higher in the experimental group, whereas unsaturated and polyunsaturated fatty acids were more abundant in the control group (P < 0.05). With regard to individual fatty acids, only C16:0 (palmitic acid), C18:2n-6 (linoleic acid) and C22:6 (docosahexaenoic acid) differed significantly. The abundance of ATP5A1 mRNA was influenced neither by diet composition nor by oocyte category (BCB<sup>+</sup>/BCB<sup>–</sup>) whereas that of the EEF1A1 was affected by both factors. Since higher mRNA level of the EEF1A1 gene was noted in BCB+ oocytes this may be considered as a marker of oocyte quality in the pig.

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Effects of vitrification on the expression of pluripotency, apoptotic and stress genes in in vitro-produced porcine blastocysts

Reproduction Fertility and Development ◽

10.1071/rd13405 ◽

2015 ◽

Vol 27 (7) ◽

pp. 1072 ◽

Cited By ~ 13

Author(s):

Miriam Castillo-Martín ◽

Marc Yeste ◽

Eva Pericuesta ◽

Roser Morató ◽

Alfonso Gutiérrez-Adán ◽

...

Keyword(s):

Superoxide Dismutase ◽

Dna Fragmentation ◽

Transcript Abundance ◽

Cell Number ◽

Total Cell ◽

Total Cell Number ◽

Altered Expression ◽

Stress Genes ◽

Relative Transcript Abundance

The aims of the present study were to: (1) evaluate the effect of vitrification and warming on quality parameters and expression levels of pluripotency, apoptotic and stress genes in in vitro-produced (IVP) porcine blastocysts; and (ii) determine the correlation between these parameters. To this end, total cell number, DNA fragmentation, peroxide levels and the relative transcript abundance of BCL-2 associated X protein (BAX), BCL2-like 1 (BCL2L1), heat shock protein 70 (HSPA1A), POU class 5 homeobox 1 (POU5F1), superoxide dismutase 1 (SOD1) and superoxide dismutase 2 (SOD2) were analysed in fresh and vitrified IVP blastocysts. The results suggest that vitrification procedures have no effect on total cell number and gene expression of BAX, BCL2L1, SOD1 and SOD2 or the BAX : BCL2L1 ratio. Nevertheless, a significant increase in DNA fragmentation (2.9 ± 0.4% vs 11.9 ± 2.0%) and peroxide levels (80.4 ± 2.6 vs 97.2 ± 3.1) were seen in vitrified compared with Day 7 fresh blastocysts. In addition, after blastocyst vitrification, relative transcript abundance was downregulated for POU5F1 and upregulated for HSPA1A. Finally, there was a significant correlation of POU5F1 and HSPA1A with DNA fragmentation (POU5F1, r = –0.561; HSPA1A, r = 0.604) and peroxide levels (POU5F1, r = –0.590; HSPA1A, r = 0.621). In conclusion, under the conditions of the present study, vitrification and warming of IVP porcine blastocysts resulted in altered expression of POU5F1 and HSPA1A, but had no effect on BAX, BCL2L1, SOD1 and SOD2 expression.

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Folliculogenesis-related genes are differently expressed in secondary and tertiary ovarian follicles

Zygote ◽

10.1017/s0967199421000265 ◽

2021 ◽

pp. 1-4

Author(s):

L.B.S. Moura ◽

D.M. Magalhães-Padilha ◽

A.N.P. Morais ◽

F.L.N. Aguiar ◽

J. Geisler–Lee ◽

...

Keyword(s):

Transcript Abundance ◽

Ovarian Follicles ◽

Mrna Abundance ◽

Qpcr Data ◽

Relative Transcript Abundance ◽

Six Genes

Summary The relative mRNA abundance of 10 genes associated with folliculogenesis was compared between late preantral (secondary) and early antral (tertiary) ovarian follicles in goats. In total, 100 follicles in each category were mechanically isolated. The relative transcript abundance of the mRNAs were determined by qPCR. Data were analyzed using unpaired Student’s t-test. Of the 10 tested genes, ABLIM mRNA was not detected in either follicle category, six genes (SLIT3, TYMS, GTPBP1, AKR1C4, PIK3R6, and MAOB) were upregulated in secondary follicles compared with tertiary follicles, and three genes (ARHGEF12, CLEC6A, and CYTL1) showed similar mRNA abundances in both secondary and tertiary follicles. In conclusion, SLIT3, GTPBP1, AKR1C4, and PIK3R6 mRNA abundance was upregulated in secondary follicles (preantral phase) compared with in tertiary follicles (antral phase) in goats.

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