Using the histone H2a transcript as an endogenous standard to study relative transcript abundance during bovine early development

2007 ◽  
Vol 74 (6) ◽  
pp. 703-715 ◽  
Author(s):  
Christian Vigneault ◽  
Isabelle Gilbert ◽  
Marc-André Sirard ◽  
Claude Robert
Keyword(s):  
Early Development ◽  
Transcript Abundance ◽  
Histone H2a ◽  
Relative Transcript Abundance

Relative transcript abundance in porcine cumulus cells collected from different size follicles

2014 ◽  
Author(s):  
Carla Moros Nicolas ◽  
Maria Jose Izquierdo Rico ◽  
Y Li ◽  
Rakel Romar ◽  
H Funahashi
Keyword(s):  
Transcript Abundance ◽  
Cumulus Cells ◽  
Relative Transcript Abundance

scholarly journals Analysis of Cadmium Root Retention for Two Contrasting Rice Accessions Suggests an Important Role for OsHMA2

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 806
Author(s):  
Moez Maghrebi ◽  
Elena Baldoni ◽  
Giorgio Lucchini ◽  
Gianpiero Vigani ◽  
Giampiero Valè ◽  
...  
Keyword(s):  
Transcript Abundance ◽  
Root Cells ◽  
Expression Levels ◽  
Cd Tolerance ◽  
Relative Contribution ◽  
Relative Transcript Abundance ◽  
Potential Capacity ◽  
Cd Distribution ◽  
Cd Exposure ◽  
Cd Availability

Two rice accessions, Capataz and Beirao, contrasting for cadmium (Cd) tolerance and root retention, were exposed to a broad range of Cd concentrations (0.01, 0.1, and 1 μM) and analyzed for their potential capacity to chelate, compartmentalize, and translocate Cd to gain information about the relative contribution of these processes in determining the different pathways of Cd distribution along the plants. In Capataz, Cd root retention increased with the external Cd concentration, while in Beirao it resulted independent of Cd availability and significantly higher than in Capataz at the lowest Cd concentrations analyzed. Analysis of thiol accumulation in the roots revealed that the different amounts of these compounds in Capataz and Beirao, as well as the expression levels of genes involved in phytochelatin biosynthesis and direct Cd sequestration into the vacuoles of the root cells, were not related to the capacity of the accessions to trap the metal into the roots. Interestingly, the relative transcript abundance of OsHMA2, a gene controlling root-to-shoot Cd/Zn translocation, was not influenced by Cd exposure in Capataz and progressively increased in Beirao with the external Cd concentration, suggesting that activity of the OsHMA2 transporter may differentially limit root-to-shoot Cd/Zn translocation in Capataz and Beirao.

scholarly journals Polymorphic Forms of Expressed Bovine Interferon-τ Genes: Relative Transcript Abundance during Early Placental Development, Promoter Sequences of Genes and Biological Activity of Protein Products*

Endocrinology ◽  
2001 ◽  
Vol 142 (7) ◽  
pp. 2906-2915 ◽  
Author(s):  
Alan D. Ealy ◽  
Sandra F. Larson ◽  
Limin Liu ◽  
Andrei P. Alexenko ◽  
Gail L. Winkelman ◽  
...  
Keyword(s):  
Biological Activity ◽  
Transcript Abundance ◽  
Placental Development ◽  
Promoter Sequences ◽  
Relative Transcript Abundance ◽  
Polymorphic Forms

Detection of abscisic acid and relative transcript abundance in Syntrichia caninervis Mitt.

2021 ◽  
pp. 1-8
Author(s):  
Yigong Zhang ◽  
Ayibaiheremu Mutailifu ◽  
Yi Zhang ◽  
Honglan Yang ◽  
Daoyuan Zhang
Keyword(s):  
Abscisic Acid ◽  
Transcript Abundance ◽  
Syntrichia Caninervis ◽  
Relative Transcript Abundance

scholarly journals Maternal nutrition affects the composition of follicular fluid and transcript content in gilt oocytes

2011 ◽  
Vol 56 (No. 4) ◽  
pp. 156-167 ◽  
Author(s):  
E. Warzych ◽  
A. Cieslak ◽  
P. Pawlak ◽  
N. Renska ◽  
E. Pers-Kamczyc ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Follicular Fluid ◽  
Diet Composition ◽  
Transcript Abundance ◽  
Marker Genes ◽  
Fluid Composition ◽  
Experimental Diet ◽  
Relative Transcript Abundance

Metabolomics focused on reproduction have been the subject of special interest in the past decade. Metabolite profiling provides detailed information on the status of follicular fluid and follicular cells which accompany the growing oocyte. Although nutrients present in the diet reach oocytes via the follicular fluid, it is not evident whether oocyte/embryo quality can be predicted based on the follicular fluid composition. Since this phenomenon has not yet been investigated in the pig, the aim of the present study was to investigate associations between diet composition and (1) selected markers related to developmental potential of oocytes (brilliant cresyl blue &ndash; BCB test, relative transcript abundance of EEF1A1 and ATP5A1 marker genes) and (2) fatty acid profile in the follicular fluid. Gilts were fed control and experimental diets which differed in composition and fatty acid profiles. The experimental diet used in this study comprised mainly locally grown grains (barley and rye) traditionally used for fattening pigs in Poland. Cumulus-oocyte complexes were aspirated from individual pairs of ovaries after animal slaughter, evaluated morphologically and subjected to the BCB test. Relative transcript abundance for the two marker genes was monitored by real-time PCR in oocytes of both categories (BCB+ and BCB&ndash;). Fatty acid profile in follicular fluid was analyzed by gas chromatography. We show that the experimental diet rich in n-3 fatty acid significantly influences fatty acid composition of the follicular fluid. The fatty acid profile of the follicular fluid of gilts fed the experimental diet differed from that of the control females. The content of saturated fatty acids was higher in the experimental group, whereas unsaturated and polyunsaturated fatty acids were more abundant in the control group (P &lt; 0.05). With regard to individual fatty acids, only C16:0 (palmitic acid), C18:2n-6 (linoleic acid) and C22:6 (docosahexaenoic acid) differed significantly. The abundance of ATP5A1 mRNA was influenced neither by diet composition nor by oocyte category (BCB<sup>+</sup>/BCB<sup>&ndash;</sup>) whereas that of the EEF1A1 was affected by both factors. Since higher mRNA level of the EEF1A1 gene was noted in BCB+ oocytes this may be considered as a marker of oocyte quality in the pig.

Effects of vitrification on the expression of pluripotency, apoptotic and stress genes in in vitro-produced porcine blastocysts

2015 ◽  
Vol 27 (7) ◽  
pp. 1072 ◽  
Author(s):  
Miriam Castillo-Martín ◽  
Marc Yeste ◽  
Eva Pericuesta ◽  
Roser Morató ◽  
Alfonso Gutiérrez-Adán ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Dna Fragmentation ◽  
Transcript Abundance ◽  
Cell Number ◽  
Total Cell ◽  
Total Cell Number ◽  
Altered Expression ◽  
Stress Genes ◽  
Relative Transcript Abundance

The aims of the present study were to: (1) evaluate the effect of vitrification and warming on quality parameters and expression levels of pluripotency, apoptotic and stress genes in in vitro-produced (IVP) porcine blastocysts; and (ii) determine the correlation between these parameters. To this end, total cell number, DNA fragmentation, peroxide levels and the relative transcript abundance of BCL-2 associated X protein (BAX), BCL2-like 1 (BCL2L1), heat shock protein 70 (HSPA1A), POU class 5 homeobox 1 (POU5F1), superoxide dismutase 1 (SOD1) and superoxide dismutase 2 (SOD2) were analysed in fresh and vitrified IVP blastocysts. The results suggest that vitrification procedures have no effect on total cell number and gene expression of BAX, BCL2L1, SOD1 and SOD2 or the BAX : BCL2L1 ratio. Nevertheless, a significant increase in DNA fragmentation (2.9 ± 0.4% vs 11.9 ± 2.0%) and peroxide levels (80.4 ± 2.6 vs 97.2 ± 3.1) were seen in vitrified compared with Day 7 fresh blastocysts. In addition, after blastocyst vitrification, relative transcript abundance was downregulated for POU5F1 and upregulated for HSPA1A. Finally, there was a significant correlation of POU5F1 and HSPA1A with DNA fragmentation (POU5F1, r = –0.561; HSPA1A, r = 0.604) and peroxide levels (POU5F1, r = –0.590; HSPA1A, r = 0.621). In conclusion, under the conditions of the present study, vitrification and warming of IVP porcine blastocysts resulted in altered expression of POU5F1 and HSPA1A, but had no effect on BAX, BCL2L1, SOD1 and SOD2 expression.

Folliculogenesis-related genes are differently expressed in secondary and tertiary ovarian follicles

Zygote ◽  
2021 ◽  
pp. 1-4
Author(s):  
L.B.S. Moura ◽  
D.M. Magalhães-Padilha ◽  
A.N.P. Morais ◽  
F.L.N. Aguiar ◽  
J. Geisler–Lee ◽  
...  
Keyword(s):  
Transcript Abundance ◽  
Ovarian Follicles ◽  
Mrna Abundance ◽  
Qpcr Data ◽  
Relative Transcript Abundance ◽  
Six Genes

Summary The relative mRNA abundance of 10 genes associated with folliculogenesis was compared between late preantral (secondary) and early antral (tertiary) ovarian follicles in goats. In total, 100 follicles in each category were mechanically isolated. The relative transcript abundance of the mRNAs were determined by qPCR. Data were analyzed using unpaired Student’s t-test. Of the 10 tested genes, ABLIM mRNA was not detected in either follicle category, six genes (SLIT3, TYMS, GTPBP1, AKR1C4, PIK3R6, and MAOB) were upregulated in secondary follicles compared with tertiary follicles, and three genes (ARHGEF12, CLEC6A, and CYTL1) showed similar mRNA abundances in both secondary and tertiary follicles. In conclusion, SLIT3, GTPBP1, AKR1C4, and PIK3R6 mRNA abundance was upregulated in secondary follicles (preantral phase) compared with in tertiary follicles (antral phase) in goats.

Relative transcript abundance of oxytocin receptor gene in porcine uterus during luteolysis and early pregnancy

2006 ◽  
Vol 47 (4) ◽  
pp. 345-351 ◽  
Author(s):  
Agnieszka Oponowicz ◽  
Anita Franczak ◽  
Beata Kurowicka ◽  
Genowefa Kotwica
Keyword(s):  
Early Pregnancy ◽  
Receptor Gene ◽  
Transcript Abundance ◽  
Oxytocin Receptor ◽  
Oxytocin Receptor Gene ◽  
Relative Transcript Abundance

scholarly journals Nano-ZnO-Induced Drought Tolerance Is Associated with Melatonin Synthesis and Metabolism in Maize

2020 ◽  
Vol 21 (3) ◽  
pp. 782 ◽  
Author(s):  
Luying Sun ◽  
Fengbin Song ◽  
Junhong Guo ◽  
Xiancan Zhu ◽  
Shengqun Liu ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Growth Regulation ◽  
Enzyme System ◽  
Transcript Abundance ◽  
Nano Zno ◽  
Melatonin Synthesis ◽  
Antioxidant Enzyme System ◽  
Relative Transcript Abundance ◽  
Underlying Mechanisms ◽  
Endogenous Melatonin

The applications of ZnO nanoparticles in agriculture have largely contributed to crop growth regulation, quality enhancement, and induction of stress tolerance, while the underlying mechanisms remain elusive. Herein, the involvement of melatonin synthesis and metabolism in the process of nano-ZnO induced drought tolerance was investigated in maize. Drought stress resulted in the changes of subcellular ultrastructure, the accumulation of malondialdehyde and osmolytes in leaf. The nano-ZnO (100 mg L−1) application promoted the melatonin synthesis and activated the antioxidant enzyme system, which alleviated drought-induced damage to mitochondria and chloroplast. These changes were associated with upregulation of the relative transcript abundance of Fe/Mn SOD, Cu/Zn SOD, APX, CAT, TDC, SNAT, COMT, and ASMT induced by nano-ZnO application. It was suggested that modifications in endogenous melatonin synthesis were involved in the nano-ZnO induced drought tolerance in maize.

Expression Patterns Of NBEAL2 In Human Tissues and a Megakaryocytic Cell Line

Blood ◽  
2013 ◽  
Vol 122 (21) ◽  
pp. 1075-1075
Author(s):  
Leila Noetzli ◽  
Natalie Smith ◽  
Gary Brodsky ◽  
Jorge Di Paola
Keyword(s):  
Skeletal Muscle ◽  
Bone Marrow ◽  
Cell Line ◽  
Transcript Abundance ◽  
Human Platelets ◽  
Human Tissues ◽  
Protein Coding ◽  
Relative Transcript Abundance ◽  
Peripheral Leukocytes ◽  
Megakaryocytic Cell

Abstract Gray platelet syndrome (GPS) is a rare autosomal recessive bleeding disorder characterized by thrombocytopenia, large platelets, and deficient alpha granules in platelets and megakaryocytes. The genetic cause of GPS was recently elucidated by our lab and others, and deleterious mutations were found in the gene NBEAL2 in several affected individuals. NBEAL2 stands for Neurobeachin-like 2 and is a large (30kb, 54 exon) gene on human chromosome 3p21.31. NBEAL2 is a member of a family of proteins that contain a BEACH (Beige and Chediak Higashi) domain which is a highly conserved domain that has been associated with protein functions such as vesicular trafficking, membrane dynamics, and receptor signaling. Very little is known about NBEAL2 other than its involvement with GPS. The relative expression of the NBEAL2 transcript, both in tissues and in subcellular compartments, is not known. It has previously been published that NBEAL2 protein coding isoforms are present in a variety of human tissues, but the abundance of NBEAL2 transcript in each tissue is not known. Furthermore, there is currently no available antibody that recognizes the 302 kDa NBEAL2 protein to validate that mRNA presence translates to protein expression. We have examined the relative transcript abundance of NBEAL2 in a human cDNA library and have validated a novel NBEAL2 antibody in a human megakaryocytic cell line (Dami) and human platelets. We obtained a human mRNA tissue library from Invitrogen which was reverse transcribed to cDNA. Tissues analyzed include bladder, brain, cervix, colon, esophagus, heart, kidney, liver, lung, ovary, placenta, prostate, skeletal muscle, small intestine, spleen, testes, thymus, thyroid, trachea, bone marrow, peripheral leukocytes, CD33+, and CD36+. To investigate relative transcript abundance, we used a Taqman qPCR probe designed to identify all six protein coding isoforms, as determined by Ensembl (http://uswest.ensembl.org). Highest NBEAL2 expression was seen in CD33+ cells, which was 54.3 fold higher than the tissue with lowest expression (skeletal muscle). Relatively high expression was also seen in the peripheral leukocytes, bone marrow, lung, esophagus, and cervix. Alternatively, NBEAL2 expression was low in the brain, despite the homology to the Neurobeachin (NBEA) brain specific protein. While high transcript abundance may infer function, relative protein expression is necessary to validate these findings. Therefore, we designed a novel NBEAL2 antibody against a 14 amino acid peptide (SLEPRRPEEAGAEVC) encoded by exon 1 of NBEAL2 that is 100% conserved between human and mouse. Western blot characterization of this antibody showed the expected approximately 300 kDa band in both soluble and insoluble Dami lysates and human platelet rich plasma lysates. Furthermore, the NBEAL2 antibody was also used for immunofluorescence of Dami cells to determine the approximate subcellular localization of the protein. Preliminary results suggest that NBEAL2 is localized to the cytoplasm, a finding that is consistent with a subcellular localization prediction program (Euk-mPLoc 2.0). In conclusion, we have determined the relative abundance of NBEAL2 transcript in several human tissues, and have begun to characterize a novel antibody against NBEAL2 using the human megakaryocytic Dami cell line and human platelets. Ongoing studies with this novel tool in the Nbeal2 knockout mouse model will likely provide new information about this elusive protein. Disclosures: Di Paola: CSL Behring: Consultancy; Pfizer: DSMB, DSMB Other.

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