scholarly journals Estradiol and Progesterone Levels are Related to Redox Status in the Follicular Fluid During In Vitro Fertilization

2020 ◽  
Vol 4 (7) ◽  
Author(s):  
Bartira Marques Pizarro ◽  
Aline Cordeiro ◽  
Mila Weydtt Reginatto ◽  
Samir P C Campos ◽  
Ana Cristina A Mancebo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Redox Status ◽  
Oocyte Quality ◽  
Fertility Treatment ◽  
Blood Contamination ◽  
Oxidative Stress Biomarkers ◽  
Vitro Fertilization

Abstract Studies have reported a possible association between the levels of oxidative stress biomarkers in follicular fluid (FF) and infertility treatment outcomes. FF analysis can provide important information about oocyte quality. This study aimed to evaluate the possible correlation between oxidative stress biomarker and intrafollicular hormone levels and clinical and laboratory parameters in women during controlled ovarian stimulation. These women were undergoing in vitro fertilization with intracytoplasmic sperm injection (ICSI).The FF samples were acquired from September 2012 to February 2014 from women undergoing private fertility treatment in Rio de Janeiro, Brazil. A total of 196 women who were undergoing ICSI and had different infertility diagnoses were recruited. The FF from each patient (average patient age of 36.3 ± 4.3 years) was collected following puncture of just one follicle with the largest diameter. After ruling out blood contamination by spectrophotometry, 163 patient samples were utilized in the study. In the FF, the progesterone levels were negatively correlated with (a) hydrogen peroxide scavenging capacity (HPSC) (r = −0.294, P < 0.0001), (b) total number of follicles (r = −0.246, P < 0.001) and (c) total number of oocytes punctured (r = −0.268, P = 0.0001). The concentration of serum estradiol exhibited a positive correlation with intrafollicular HPSC (r = 0.165, P = 0.037). Our data indicate that the FF levels of estradiol and progesterone are related to the FF redox status, which is closely associated with the number of oocytes obtained during ICSI procedures.

Oxidative stress biomarkers in newborn calves: Comparison among artificial insemination, in vitro fertilization and cloning

2020 ◽  
Vol 219 ◽  
pp. 106538 ◽  
Author(s):  
Gabriela N. Dantas ◽  
Bianca P. Santarosa ◽  
Vitor H. Santos ◽  
Henrique B. Hooper ◽  
Ricardo A. Micai ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Artificial Insemination ◽  
Oxidative Stress Biomarkers ◽  
Vitro Fertilization ◽  
Stress Biomarkers ◽  
Newborn Calves

scholarly journals From “Every Day” Hormonal to Oxidative Stress Biomarkers in Blood and Follicular Fluid, to Embryo Quality and Pregnancy Success?

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Katarzyna Olszak-Wąsik ◽  
Anna Bednarska-Czerwińska ◽  
Anita Olejek ◽  
Andrzej Tukiendorf
Keyword(s):  
Oxidative Stress ◽  
Follicular Fluid ◽  
Embryo Quality ◽  
R Package ◽  
Single Embryo Transfer ◽  
Oxidative Stress Biomarkers ◽  
Oocyte Growth ◽  
Vitro Fertilization ◽  
Stress Markers

Background. Oxidative stress appears to be involved in oocyte growth and maturation that when impaired results in poor embryo quality and lower potential to implant. The biochemical microenvironment of the oocyte (follicular fluid (FF)) consists of hormones and other various substances regulating the balance between oxidants and antioxidants. Aim. The aim of this study was to examine the possible impact of selected biomarkers (“every day,” hormonal biomarkers, enzymatic and nonenzymatic antioxidants, and also oxidative stress markers) in serum and FF, on embryo quality and pregnancy success in infertile women undergoing infertility treatment. Methods. All 53 patients, mean age 34.7±4.1 years, with serum AMH level≥0.7 ng/mL, were diagnosed with idiopathic infertility. They were stimulated in short antagonist protocol, followed by in vitro fertilization (IVF-ICSI intracytoplasmatic sperm injection) and a single embryo transfer. Follicular fluid was aspirated from the first mature follicle. In statistical analyses the R software was used, then all data was assessed with the Shapiro-Wilk test, logistic regression, and later the receiver operating characteristic (ROC) curve was applied using “pROC” R package. Results. We did not observe any correlation between AMH and embryo quality and pregnancy rate. Statistically significant results were only found for biomarkers examined in follicular fluid. Greater levels of GPX in FF were associated with the increased chance of producing a high quality embryo (the optimal cut-off concentration was established at over 450 lU/L.) Regarding pregnancy success, increasing levels of GR (cut-off at 21 IU/L), CuZnSOD (cut-off at 9NU/mL), and GST (cut-off at 2.5 IU/L) resulted in lower chances of a successful pregnancy. Conclusion. Our results indicate that FF markers may have some advantages in predicting embryo quality and pregnancy over AMH. The GPX system seems to be mostly related to embryo quality and pregnancy.

Obesity is not a descriptive factor for oxidative stress in follicular fluid of in-vitro fertilization patients

2013 ◽  
Vol 100 (3) ◽  
pp. S425
Author(s):  
B. Yuksel ◽  
S. Kilic ◽  
N. Yilmaz ◽  
T. Goktas ◽  
M. Ulubay ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Vitro Fertilization

Oxidative stress indices in follicular fluid as measured by the thermochemiluminescence assay correlate with outcome parameters in in vitro fertilization

2004 ◽  
Vol 82 ◽  
pp. 1171-1176 ◽  
Author(s):  
Zofnat Wiener-Megnazi ◽  
Liad Vardi ◽  
Arie Lissak ◽  
Sergei Shnizer ◽  
Abraham Zeev Reznick ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Vitro Fertilization ◽  
Stress Indices ◽  
Outcome Parameters

scholarly journals Oxidative stress and DNA damage status in couples undergoing in vitro fertilization treatment

2021 ◽  
Author(s):  
Iman Al-Saleh ◽  
Serdar Coskun ◽  
Reem Al-Rouqi ◽  
Tahreer Al-Rajudi ◽  
Chafica Eltabache ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
In Vitro Fertilization ◽  
Seminal Plasma ◽  
Follicular Fluid ◽  
Fertilization Rate ◽  
Reproductive Hormones ◽  
Vitro Fertilization ◽  
Poor Fertilization

This study examined the status of oxidative stress in 599 couples undertaking in vitro fertilization (IVF) treatment and its association with reproductive hormones, smoking, and outcomes. Oxidative stress biomarkers such as malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), hydrogen peroxide (H2O2), catalase (CAT), and total antioxidant capacity (TAC) were determined in follicular fluid and seminal plasma. Tail moment (TM) was used to evaluate DNA damage in sperm and granulosa cells. Reproductive hormones in serum and cotinine (COT) in urine, follicular fluid, and seminal plasma samples were determined. We used log-binomial multivariate regression to estimate relative risks for the association between oxidative stress/DNA damage and IVF binary outcomes (fertilization rate, biochemical pregnancy, clinical pregnancy, and live birth). We observed an increase in the oxidative stress markers MDA, 8-OHdG, and H2O2 in follicular fluid and seminal plasma, but a decrease in the antioxidant protection markers CAT and TAC. The MDA, 8-OHdG, and H2O2 levels were significantly higher in seminal plasma than in follicular fluid, while TAC, CAT, and TM were higher in follicular fluid (p < 0.001). Although women were nonsmokers, COT levels >50 µg/l were observed in 5.7% (urine) and 1.4% (follicular fluid). An increase in the CAT levels of follicular fluid was associated with a 48 and 41% decrease in the risk of poor fertilization rate (≤50%) and unsuccessful live birth, respectively. After the models were adjusted for hormonal factors, the associations remained the same, except that elevated TAC in follicular fluid became significantly associated with a decrease of 42% in the risk of poor fertilization rate (≤50%).

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