Obesity is not a descriptive factor for oxidative stress and viscosity in follicular fluid of in vitro fertilization patients

2016 ◽  
Vol 186 (3) ◽  
pp. 641-646
Author(s):  
B. Yuksel ◽  
S. Kilic ◽  
N. Yilmaz ◽  
T. Goktas ◽  
U. Keskin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Vitro Fertilization

scholarly journals Estradiol and Progesterone Levels are Related to Redox Status in the Follicular Fluid During In Vitro Fertilization

2020 ◽  
Vol 4 (7) ◽  
Author(s):  
Bartira Marques Pizarro ◽  
Aline Cordeiro ◽  
Mila Weydtt Reginatto ◽  
Samir P C Campos ◽  
Ana Cristina A Mancebo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Redox Status ◽  
Oocyte Quality ◽  
Fertility Treatment ◽  
Blood Contamination ◽  
Oxidative Stress Biomarkers ◽  
Vitro Fertilization

Abstract Studies have reported a possible association between the levels of oxidative stress biomarkers in follicular fluid (FF) and infertility treatment outcomes. FF analysis can provide important information about oocyte quality. This study aimed to evaluate the possible correlation between oxidative stress biomarker and intrafollicular hormone levels and clinical and laboratory parameters in women during controlled ovarian stimulation. These women were undergoing in vitro fertilization with intracytoplasmic sperm injection (ICSI).The FF samples were acquired from September 2012 to February 2014 from women undergoing private fertility treatment in Rio de Janeiro, Brazil. A total of 196 women who were undergoing ICSI and had different infertility diagnoses were recruited. The FF from each patient (average patient age of 36.3 ± 4.3 years) was collected following puncture of just one follicle with the largest diameter. After ruling out blood contamination by spectrophotometry, 163 patient samples were utilized in the study. In the FF, the progesterone levels were negatively correlated with (a) hydrogen peroxide scavenging capacity (HPSC) (r = −0.294, P < 0.0001), (b) total number of follicles (r = −0.246, P < 0.001) and (c) total number of oocytes punctured (r = −0.268, P = 0.0001). The concentration of serum estradiol exhibited a positive correlation with intrafollicular HPSC (r = 0.165, P = 0.037). Our data indicate that the FF levels of estradiol and progesterone are related to the FF redox status, which is closely associated with the number of oocytes obtained during ICSI procedures.

Obesity is not a descriptive factor for oxidative stress in follicular fluid of in-vitro fertilization patients

2013 ◽  
Vol 100 (3) ◽  
pp. S425
Author(s):  
B. Yuksel ◽  
S. Kilic ◽  
N. Yilmaz ◽  
T. Goktas ◽  
M. Ulubay ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Vitro Fertilization

Oxidative stress indices in follicular fluid as measured by the thermochemiluminescence assay correlate with outcome parameters in in vitro fertilization

2004 ◽  
Vol 82 ◽  
pp. 1171-1176 ◽  
Author(s):  
Zofnat Wiener-Megnazi ◽  
Liad Vardi ◽  
Arie Lissak ◽  
Sergei Shnizer ◽  
Abraham Zeev Reznick ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Vitro Fertilization ◽  
Stress Indices ◽  
Outcome Parameters

scholarly journals Oxidative stress and DNA damage status in couples undergoing in vitro fertilization treatment

2021 ◽  
Author(s):  
Iman Al-Saleh ◽  
Serdar Coskun ◽  
Reem Al-Rouqi ◽  
Tahreer Al-Rajudi ◽  
Chafica Eltabache ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
In Vitro Fertilization ◽  
Seminal Plasma ◽  
Follicular Fluid ◽  
Fertilization Rate ◽  
Reproductive Hormones ◽  
Vitro Fertilization ◽  
Poor Fertilization

This study examined the status of oxidative stress in 599 couples undertaking in vitro fertilization (IVF) treatment and its association with reproductive hormones, smoking, and outcomes. Oxidative stress biomarkers such as malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), hydrogen peroxide (H2O2), catalase (CAT), and total antioxidant capacity (TAC) were determined in follicular fluid and seminal plasma. Tail moment (TM) was used to evaluate DNA damage in sperm and granulosa cells. Reproductive hormones in serum and cotinine (COT) in urine, follicular fluid, and seminal plasma samples were determined. We used log-binomial multivariate regression to estimate relative risks for the association between oxidative stress/DNA damage and IVF binary outcomes (fertilization rate, biochemical pregnancy, clinical pregnancy, and live birth). We observed an increase in the oxidative stress markers MDA, 8-OHdG, and H2O2 in follicular fluid and seminal plasma, but a decrease in the antioxidant protection markers CAT and TAC. The MDA, 8-OHdG, and H2O2 levels were significantly higher in seminal plasma than in follicular fluid, while TAC, CAT, and TM were higher in follicular fluid (p < 0.001). Although women were nonsmokers, COT levels >50 µg/l were observed in 5.7% (urine) and 1.4% (follicular fluid). An increase in the CAT levels of follicular fluid was associated with a 48 and 41% decrease in the risk of poor fertilization rate (≤50%) and unsuccessful live birth, respectively. After the models were adjusted for hormonal factors, the associations remained the same, except that elevated TAC in follicular fluid became significantly associated with a decrease of 42% in the risk of poor fertilization rate (≤50%).

scholarly journals Obesity leads to distinct metabolomic signatures in follicular fluid of women undergoing in vitro fertilization

2019 ◽  
Vol 316 (3) ◽  
pp. E383-E396 ◽  
Author(s):  
Meghan L. Ruebel ◽  
Brian D. Piccolo ◽  
Kelly E. Mercer ◽  
Lindsay Pack ◽  
Dean Moutos ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mass Spectrometry ◽  
Lipid Metabolism ◽  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Time Of Flight ◽  
Unknown Primary ◽  
Vitro Fertilization ◽  
Complex Lipids

Although obesity negatively influences the metabolic homeostasis of cells within a broad range of tissues, its impact on oocyte metabolism is not fully understood. Prior evidence suggests that obesity increases expression of oocyte genes associated with inflammation, oxidative stress, and lipid metabolism; however, the metabolic impact of these genetic differences is not known. To address this gap, we conducted an exploratory assessment of the follicular fluid (FF) metabolome in eight overweight/obese (OW) and nine normal-weight (NW) women undergoing in vitro fertilization. FF and serum were collected and analyzed by untargeted metabolomics using gas chromatography-quadrupole time-of-flight mass spectrometry and charged-surface hybrid column-electrospray ionization quadrupole time-of-flight tandem mass spectrometry. Untargeted metabolomics identified obesity-associated changes in FF metabolites related to oxidative stress/antioxidant capacity, xenometabolism/amino acid biosynthesis, and lipid metabolism. Discriminant FF metabolites included elevated uric acid, isothreonic acid, one unknown primary metabolite, and six unknown complex lipids in OW compared with NW women. Conversely, 2-ketoglucose dimethylacetal, aminomalonate, two unknown primary metabolites, and two unknown complex lipids were decreased in FF of OW relative to NW women. Indole-3-propionic acid (IPA), a bacteria-derived metabolite, was also decreased in both FF and serum of OW women ( P < 0.05). The significant correlation between antioxidant IPA in serum and FF ( R = 0.95, P < 0.0001) suggests a potential serum biomarker of FF antioxidant status or reflection of the gut metabolism interaction with the follicle. These results suggest that obesity has important consequences for the follicular environment during the preconception period, a window of time that may be important for lifestyle interventions to ameliorate obesity-associated risk factors.

scholarly journals The effect of follicular fluid selenium concentration on oocyte maturation in women with polycystic ovary syndrome undergoing in vitro fertilization/Intracytoplasmic sperm injection: A cross-sectional study

2021 ◽  
pp. 689-698
Author(s):  
Seyedeh Mahsa Poormoosavi ◽  
Mohammad Amin Behmanesh ◽  
Hossein Najafzadeh Varzi ◽  
Shabnam Mansouri ◽  
Sima Janati
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Polycystic Ovary Syndrome ◽  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Selenium Concentration ◽  
Ovary Syndrome ◽  
Cross Sectional ◽  
Vitro Fertilization

Background: A high level of free radicals and oxidative substances in women with polycystic ovary syndrome (PCOS) can affect the ovaries through oxidative stress. Antioxidants such as selenium, a vital trace element in human health, can improve the prognosis of PCOS by reducing oxidative stress. Objective: This study was performed due to the lack of comprehensive information about selenium concentration in follicular fluid and its effect on the oocyte count and quality in infertile women with PCOS. Materials and Methods: In this cross-sectional study, 78 women with PCOS referred to Umm-al-Banin Infertility Clinic Center, Ganjavian Hospital, Dezful, Iran for in-vitro fertilization from March to November 2019 were enrolled. After ovarian stimulation with the antagonist protocol, the oocytes were retrieved under transvaginal ultrasound in in-vitro fertilization/intracytoplasmic sperm injection cycles, and selenium concentrations were measured in the follicular fluid using an atomic absorption method by spectrophotometer device. Oocyte count and morphology were evaluated using inverted optical microscopy. Results: There were no significant differences between follicular fluid selenium concentrations in terms of the total number of oocytes and immature oocytes in the metaphase I and germinal vesicle stages. However, a significantly reduced number of metaphase II oocytes was observed at selenium levels < 40 μg/dL (p = 0.001). Conclusion: Based on our results, low levels of follicular selenium concentration in infertile women with PCOS can reduce the quality and potency of oocyte maturation. Key words: Polycystic ovary syndrome, Oxidative stress, Selenium, In vitro fertilization, Oocyte quality, Follicular fluid.

Sign in / Sign up

Export Citation Format

Share Document