scholarly journals Prolactin Enhances CCAAT Enhancer-Binding Protein-β (C/EBPβ) and Peroxisome Proliferator-Activated Receptor γ (PPARγ) Messenger RNA Expression and Stimulates Adipogenic Conversion of NIH-3T3 Cells

2000 ◽  
Vol 14 (2) ◽  
pp. 307-316 ◽  
Author(s):  
Rika Nanbu-Wakao ◽  
Yoshio Fujitani ◽  
Yasuhiko Masuho ◽  
Masa-aki Muramatu ◽  
Hiroshi Wakao
Keyword(s):  
Messenger Rna ◽  
Binding Protein ◽  
Rna Expression ◽  
Peroxisome Proliferator ◽  
3T3 Cells ◽  
Peroxisome Proliferator Activated Receptor ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein ◽  
Nih 3T3 ◽  
Nih 3T3 Cells

scholarly journals Combined Anti-Adipogenic Effects of Hispidulin and p-Synephrine on 3T3-L1 Adipocytes

Biomolecules ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1764
Author(s):  
Dahae Lee ◽  
Hee Jae Kwak ◽  
Byoung Ha Kim ◽  
Seung Hyun Kim ◽  
Dong-Wook Kim ◽  
...  
Keyword(s):  
Synergistic Effects ◽  
Binding Protein ◽  
Pharmacokinetic Parameters ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein ◽  
Mitogen Activated Protein ◽  
Human Participants ◽  
Arrabidaea Chica

Hispidulin is abundant in Arrabidaea chica, Crossostephium chinense, and Grindelia argentina, among others. p-Synephrine is the main phytochemical constituent of Citrus aurantium. It has been used in combination with various other phytochemicals to determine synergistic effects in studies involving human participants. However, there have been no reports comparing the anti-adipogenic effects of the combination of hispidulin and p-synephrine. The current study explores the anti-adipogenic effects of hispidulin alone and in combination with p-synephrine in a murine preadipocyte cell line, 3T3-L1. Co-treatment resulted in a greater inhibition of the formation of red-labeled lipid droplets than the hispidulin or p-synephrine-alone treatments. Co-treatment with hispidulin and p-synephrine also significantly inhibited adipogenic marker proteins, including Akt, mitogen-activated protein kinases, peroxisome proliferator-activated receptor gamma, CCAAT/enhancer-binding protein alpha, glucocorticoid receptor, and CCAAT/enhancer-binding protein β. Although further studies are required to assess the effects of each drug on pharmacokinetic parameters, a combination treatment with hispidulin and p-synephrine may be a potential alternative strategy for developing novel anti-obesity drugs.

scholarly journals Combined Beneficial Effect of Genistein and Atorvastatin on Adipogenesis in 3T3-L1 Adipocytes

Biomolecules ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1052
Author(s):  
Dahae Lee ◽  
Ji-Youn Kim ◽  
Hae-Won Kim ◽  
Jeong-Eun Yoo ◽  
Ki Sung Kang
Keyword(s):  
Binding Protein ◽  
Inhibitory Effect ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Marker Proteins ◽  
Low Concentrations ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein ◽  
Mitogen Activated Protein ◽  
Combinational Treatment

Genistein (4,5,7-trihydroxyisoflavone) is abundant in various dietary vegetables, especially soybeans, and is known to have not only an estrogenic effect but also an antiadipogenic effect. Atorvastatin (dihydroxy monocarboxylic acid) is a statin used to prevent heart disease. Although genistein and atorvastatin have been reported to possess antiadipogenic effects, their combined effects are still unclear. The aim of the current study was to explore whether the combination of genistein and atorvastatin at low concentrations significantly suppresses adipogenesis in a murine preadipocyte cell line (3T3-L1) compared to treatment with genistein or atorvastatin alone. Our results showed that cotreatment with 50 µM genistein and 50 nM atorvastatin significantly suppressed preadipocyte differentiation, whereas when each compound was used alone, there was no inhibitory effect. Additionally, cotreatment with genistein and atorvastatin significantly downregulated adipogenic marker proteins, including mitogen-activated protein kinases (MAPKs), peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), glucocorticoid receptor (GR), and CCAAT/enhancer-binding protein β (C/EBPβ). This is the first evidence of the combined antiadipogenic effects of genistein and atorvastatin. Although additional experiments are required, combinational treatment with genistein and atorvastatin may be an alternative treatment for menopause-associated lipid metabolic disorders and obesity.

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