Estrogen and Progesterone Receptors in Meningiomas: Comparison of Nuclear Binding, Dextran-Coated Charcoal, and Immunoperoxidase Staining Assays

Neurosurgery ◽  
1989 ◽  
Vol 25 (4) ◽  
pp. 546-553 ◽  
Author(s):  
Jaroslava Halper ◽  
Douglas S. Colvard ◽  
Bernd W. Scheithauer ◽  
Nai-Siang Jiang ◽  
Michael F. Press ◽  
...  
Keyword(s):  
Estrogen Receptors ◽  
Steroid Receptors ◽  
Progesterone Receptors ◽  
Immunoperoxidase Staining ◽  
Estrogen And Progesterone Receptors ◽  
High Affinity ◽  
Nuclear Binding ◽  
Dextran Coated Charcoal ◽  
The Status

Abstract We studied the status of estrogen (ER) and progesterone (PR) receptors in meningiomas removed from 52 patients, comparing dextran-coated charcoal (DCC), nuclear binding (NB), and immunoperoxidase (IP) assays. Each of the assays was performed independently by investigators well-experienced with these assays. The NB assay is a new assay that measures functional steroid receptors—that is, the activation of the receptor and its binding to the nucleus. The assay is very sensitive and requires a relatively small amount of tissue as compared with the DCC assay. In agreement with data from other studies, PR were detected in most meningiomas by all 3 methods: in 69% of the cases by NB, in 76% by DCC, and in 89% by IP. ER were detected in only a few cases: in 33% by NB, in 2% by DCC, and in none by the IP assay. The agreement for PR sites was 62% for all 3 assays, it was 66% between the NB and DCC assays, 67% between the NB and IP assays, and 86% between the DCC and IP assays. Of 26 cases that were positive by the DCC assay, 6 (23%) were negative by NB. The overall agreement for all three ER assays was 65%. The data suggest that the majority of meningiomas contain high-affinity receptors for progesterone, that estrogen receptors are present in only a few meningiomas, and that some of these estrogen and progesterone receptors appear to be functional.

Hormonal dependency of cerebral meningiomas

1990 ◽  
Vol 73 (5) ◽  
pp. 743-749 ◽  
Author(s):  
Uwe M. H. Schrell ◽  
Eric F. Adams ◽  
Rudolf Fahlbusch ◽  
Robert Greb ◽  
Gustav Jirikowski ◽  
...  
Keyword(s):  
Estrogen Receptors ◽  
Binding Sites ◽  
Solid Phase ◽  
Steroid Receptors ◽  
Progesterone Receptors ◽  
Nuclear Compartment ◽  
Content Type ◽  
High Affinity ◽  
Immunohistochemical Techniques ◽  
Fine Print

✓ Female sex steroid receptors were examined in 50 human cerebral meningiomas. For estrogen receptors, high-affinity binding sites (dissociation constant (Kd): 0.05 to 0.2 nM) were found in the cytosolic fraction with a capacity of less than 4 fmol/mg protein in 10 meningiomas using a dextran-coated charcoal (DCC) assay. In the same cytosolic fraction, the solid-phase enzyme immunoassay revealed only one cytosol with a positive colorimetric reaction equal to 5 fmol/mg protein. However, in the nuclear compartment, none of the tumors stained positively for estrogen receptors with immunohistochemical techniques. In addition, the most convincing evidence for the absence of estrogen receptors was obtained by in situ hybridization using an oligonucleotide probe complementary to a fraction of the human receptor messenger ribonucleic acid (mRNA). In none of the 50 meningiomas was the expression of estrogen mRNA coding for the estrogen receptor detected. For progesterone receptors, high-affinity binding sites (Kd: 0.3 to 2.6 nM) were found in 49 of the 50 tumors using a DCC assay. In the same cytosols, solid-phase enzyme immunoassay revealed that each tumor was positive for progesterone receptors. However, in the nuclear compartment, only five tumors had partially positive staining for progesterone receptors with immunohistochemical techniques. Within the confines of this study, it is concluded that: 1) the estrogen receptor is generally absent in meningioma tissue, and 2) the progesterone receptor is mainly absent in the nuclear compartment, leading to the conclusion that the cytosolic progesterone receptor may be an inactive form. This study suggests that female sex steroid receptors are not primarily involved in the proliferative rate of cerebral meningiomas and that they are of no current significance as markers for adjuvant medical therapy of most meningiomas.

Simultaneous measurement of estrogen and progesterone receptors in tumor cytosols with use of 125I-labeled estradiol and of 3H-R5020.

1981 ◽  
Vol 27 (5) ◽  
pp. 687-691 ◽  
Author(s):  
S N Thibodeau ◽  
L Freeman ◽  
N S Jiang
Keyword(s):  
Estrogen Receptors ◽  
Simultaneous Measurement ◽  
Liquid Scintillation ◽  
Scintillation Counter ◽  
Progesterone Receptors ◽  
Scatchard Plot ◽  
Estrogen And Progesterone Receptors ◽  
Dual Isotope ◽  
Dextran Coated Charcoal ◽  
Dual Label

Abstract We describe a dual-isotope assay for measuring the concentration of estrogen receptors and progesterone receptors in tumor cytosols. The concentrations of these receptors are derived from separate Scatchard-plot analyses of a single dextran-coated charcoal assay that incorporates both radioiodinated estradiol and tritiated R5020 as the labeled ligands. The two isotopes, radioiodine and tritium, are easily measured with a liquid-scintillation counter. The concentrations of estrogen and progesterone receptors derived from the dual-label assay are identical to values derived from the respective single-label assays. Moreover, values obtained from an assay with dextran-coated charcoal that incorporates radioiodinated estradiol are identical to those obtained from an assay in which tritiated estradiol is used as the labeled ligand. The dual-isotope assay requires both less time and less tissue.

scholarly journals Investigating the Factors Associated with the Level of Expression of Estrogen and Progesterone Receptors in Patients Suffering from Colorectal Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Saleheh Salehi far ◽  
Maryam Soltani ◽  
Mahmoud Zardast ◽  
Mohammad Reza Ghasemian Moghaddam
Keyword(s):  
Colorectal Cancer ◽  
Drugs Of Abuse ◽  
Progesterone Receptors ◽  
Advanced Stage ◽  
Estrogen And Progesterone Receptors ◽  
Familial History ◽  
Factors Associated ◽  
The Status ◽  
The Mean ◽  
Estrogen And Progesterone Receptor

Background. The present study was performed to investigate the factors related to the expression level of estrogen and progesterone receptor in patients with colorectal cancer. Material and Methods. This crosssectional study was performed on 54 patients suffering from colorectal cancer referring to Imam Reza Hospital in Birjand during 2018-2019. After the biopsy performed during surgery, the specimen was sent for immunohistochemistry, and the status of receptors was determined. Eventually, the data were analyzed by SPSS 22. Results. Out of the 54 patients studied, 64.8% were male. The mean age of the patients was 62.28 ± 14.03 years. The level of expression of beta-estrogen receptors and progesterone receptors had a significant relationship with age, consuming drugs of abuse, and familial history ( P = 0.001 ). Also, the level of expression of estrogen and progesterone receptors of patients with a more advanced stage of cancer was significantly lower ( P = 0.001 ). Conclusion. The extent of expression of estrogen and progesterone receptors affects the progression and prognosis of disease. Thus, through hormone therapy, a step can be taken to reduce the progression and even to treat colorectal cancer.

scholarly journals Morphological Characteristics and Expression of Estrogen and Progesterone Receptors in the Canine Endometrium During the Estrus Cycle, Cystic Endometrial Hyperplasia and Pyometra

2018 ◽  
Vol 68 (3) ◽  
pp. 239-250
Author(s):  
Darko Marinković ◽  
Milan Aničić ◽  
Slobodanka Vakanjac ◽  
Svetlana Nedić ◽  
Vladimir Magaš
Keyword(s):  
Estrogen Receptors ◽  
Endometrial Hyperplasia ◽  
Progesterone Receptors ◽  
Morphological Characteristics ◽  
Estrus Cycle ◽  
Estrogen And Progesterone Receptors ◽  
Strong Reaction ◽  
Weak Reaction ◽  
Moderate Reaction ◽  
Cyclic Changes

Abstract The estrus cycle of bitches is divided into four phases: proestrus, estrus, diestrus and anestrus, during which different morphological changes, and also cyclic changes of estrogen and progesterone receptors are present. Several pathological changes can be differentiated on the endometrium, but one of these is the most important - cystic endometrial hyperplasia, which frequently develops into pyometra. The aim of the present study was to describe morphological characteristics, and expression of estrogen and progesterone receptors on the endometrium of mixedbreed bitches during the different phases of the estrus cycle, cystic endometrial hyperplasia and pyometra. The uterus and ovaries of 36 mixed breed bitches in different phases of the estrus cycle and also with cystic endometrial hyperplasia (CEH) and chronic purulent endometritis - pyometra were examined macroscopically, histopathologically, and immunohistochemically for estrogen receptors (ΕR) and progesterone receptors (PR). During proestrus uterine cells showed a weak reaction for both estrogen and progesterone receptors, but during estrus a large number of uterine cells showed a strong reaction on estrogen receptors and moderate reaction on progesterone receptors. On the contrary, during diestrus the scores for the estrogen receptors decreased, while the progesterone receptors level increased - uterine cells expressed strong reaction for progesterone receptors, and moderate reaction for estrogen receptors. Uterine cells in cystic endometrial hyperplasia expressed a strong reaction for estrogen receptors, and moderate reaction for progesterone receptors, but on the other hand the uterine cells in the uterus with pyometra expressed a moderate to strong reaction for progesterone receptors, and a weak reaction for estrogen receptors. In further investigations it would be interesting to perform quantitative analysis for both estrogen and progesterone receptors during different phases of the estrus cycle and also in the uterus with cystic endometrial hyperplasia and pyometra.

Estrogen and Progesterone Receptors in Human Colorectal Tumour Cells (Study of 70 Cases)

1988 ◽  
Vol 3 (1) ◽  
pp. 41-48 ◽  
Author(s):  
G. Bracali ◽  
A.M. Caracino ◽  
F. Rossodivita ◽  
C. Bianchi ◽  
M.G. Loli ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Estrogen Receptor ◽  
Progesterone Receptor ◽  
Estrogen Receptors ◽  
Progesterone Receptors ◽  
Human Colorectal Cancer ◽  
Estrogen And Progesterone Receptors ◽  
Colorectal Tumour ◽  
Estrogen Receptor Positive ◽  
Cytochemical Technique

Estrogen and progesterone receptors were studied in 70 cases of human colorectal cancer by a cytochemical technique. 28.5% of the cases were estrogen-receptor positive and 42.8% progesterone-receptor positive. There was no difference between the sexes for estrogen receptors but the women had more tumours with progesterone receptors than men. The presence of receptors is unrelated to the differentiation of the tumour. More colon tumours were positive than those of the sigma and rectum. The concentration of cells with receptors in positive cancer cases tended to be low or medium-low.

Microassay for nuclear binding of steroid receptors with use of intact cells from small samples of avian and human tissue.

1988 ◽  
Vol 34 (2) ◽  
pp. 363-369 ◽  
Author(s):  
D S Colvard ◽  
W R Jankus ◽  
N J Berg ◽  
M L Graham ◽  
N S Jiang ◽  
...  
Keyword(s):  
Binding Assay ◽  
Steroid Receptors ◽  
Progesterone Receptors ◽  
Small Samples ◽  
Intact Cells ◽  
Nuclear Binding ◽  
Assay Tube ◽  
Estrogen Receptor Positive ◽  
Small Needle ◽  
Endometrial Carcinomas

Abstract A micro version of a nuclear binding assay to assess the biological activity of receptors for steroid hormones was developed for application to small (needle) biopsies of human tumors for the purpose of predicting responses to steroid therapy. This easier assay requires 10-fold less tissue than the original nuclear binding assay described for progesterone receptors in the avian oviduct, endometrium, and endometrial carcinomas (Spelsberg TC, et al., Endocrinology 1987;121:631). We describe the application of this micro assay to normal avian oviduct and cancers of the human breast, and we demonstrate a tissue specificity and saturation of nuclear binding. The micro assay reliably measured as little as 0.5 mg equivalents of tissue per assay tube. Results for breast tumors determined to be estrogen-receptor-positive by the standard dextran-coated charcoal method were also determined with this nuclear binding assay. As described previously for progesterone receptors in endometrial carcinomas, some receptor-positive breast biopsies displayed negligible capacity for nuclear binding. Therefore, with the present assay we have identified nonfunctional receptors in these biopsies, which may be useful for accurate prediction of patients' responses to therapy with hormones.

Estrogen and Progesterone Receptors in Breast Carcinoma and in Nonmalignant Breast Tissue

1985 ◽  
Vol 71 (5) ◽  
pp. 477-481 ◽  
Author(s):  
Massimo Gion ◽  
Riccardo Mione ◽  
Ruggero Dittadi ◽  
Luciano Griggio ◽  
Gabriele Munegato ◽  
...  
Keyword(s):  
Breast Carcinoma ◽  
Estrogen Receptors ◽  
Breast Tissue ◽  
Progesterone Receptors ◽  
Estrogen And Progesterone Receptors ◽  
Receptor Level ◽  
Receptor Status ◽  
Benign Tissue ◽  
Receptor Concentration ◽  
The Relationship

Since 1983 we have studied the relationship, in the same patient, between receptor status in breast carcinoma and in nonmalignant breast tissue. Fifty patients have been evaluated to date. The total unoccupied cytosol estrogen and progesterone receptors were determined by a dextran-coated charcoal method. In nonmalignant breast tissue we found a measurable receptor concentration above the sensitivity of the method in 62 % of cases for estrogen receptors and in 44 % of cases for progesterone receptors. No relationships were found between the receptor level of each tumor and that of the corresponding benign tissue. The data suggest that the levels of the receptors in the tumor and in the nonmalignant tissue are totally independent.

Steroid Hormone Receptors in Laryngeal Carcinoma

1986 ◽  
Vol 94 (4) ◽  
pp. 512-517 ◽  
Author(s):  
Erkki Virolainen ◽  
Pekka Tuohimaa ◽  
Kalle Aitasalo ◽  
Jyrki Kyttä ◽  
Riitta Vanharanta-Hiltunen
Keyword(s):  
Estrogen Receptors ◽  
Laryngeal Carcinoma ◽  
Androgen Receptors ◽  
Progesterone Receptors ◽  
Steroid Hormone Receptors ◽  
Estrogen And Progesterone Receptors ◽  
Carcinoma Tissue ◽  
Squamous Carcinoma Cells ◽  
Laryngeal Mucosa ◽  
Laryngeal Carcinomas

The larynx has long been shown to be a target organ for androgenic steroids in both women and men, and specific androgen receptors have been determined in normal laryngeal mucosa and in laryngeal carcinoma tissue. In this study, samples from 21 primary laryngeal carcinomas, from 4 recurrent laryngeal carcinomas and from 1 cervical metastasis of laryngeal carcinoma were obtained at the time of surgery to assay specific androgen, estrogen, and progesterone receptors. Specific androgen receptors were found in 8 samples (31%). The level of receptors varied from 1.7 femtomoles (fmol) per milligram to 7.3 fmol/mg cytosol protein. Detectable levels of specific estrogen receptors were found in 18 samples (69%) and progesterone receptors in 8 of the 15 samples studied (53%). There was no apparent correspondence with donors’ sex, since samples from both females and males contained all kinds of receptors. We know that antiestrogen inhibits the growth of squamous carcinoma cells lines positive for estrogen receptors in vitro and that this effect is reversible with the appropriate hormone. Thus, the relatively high percentage of estrogen and progesterone receptors found in laryngeal carcinoma tissue may open new aspects in the treatment of laryngeal carcinoma with antihormones.

scholarly journals An immunohistochemical analysis of sex-steroid receptors, tumor suppressor gene p53 and Ki-67 in the normal and neoplastic uterine cervix squamous epithelium

2014 ◽  
Vol 67 (7-8) ◽  
pp. 202-207 ◽  
Author(s):  
Marinos Nikolaou ◽  
Dimitra Koumoundourou ◽  
Panagiota Ravazoula ◽  
Margarita Papadopoulou ◽  
Georgios Michail ◽  
...  
Keyword(s):  
Estrogen Receptors ◽  
Cervical Intraepithelial Neoplasia ◽  
Cervical Carcinoma ◽  
Steroid Receptors ◽  
Progesterone Receptors ◽  
Intraepithelial Neoplasia ◽  
Sex Steroid ◽  
Ki 67 ◽  
Sex Steroid Receptors ◽  
Squamous Cervical Carcinoma

Introduction. Malignant transformation of sex-steroid dependent tissues is associated with the loss of expression of sex steroid receptors as well as of the tumor suppression gene p53. The aim of this study is to evaluate the expression of sex-steroid receptors, p53 and Ki-67 in specimens from pre-malignant and malignant cervical epithelial lesions throughout the menstrual cycle. Material and Methods. Immunohistochemical staining was performed on formalin fixed, paraffin embedded tissue sections of normal squamous cervical epithelium, cervical intraepithelial neoplasia and invasive squamous cervical carcinoma, specimens utilizing antibodies against estrogen receptors, progesterone receptors, p53 protein and Ki-67 antigen. Results. In the samples taken from the normal cervical tissue, basal cells were usually estrogen receptor-positive, progesterone receptornegative, p53-negative and Ki-67-negative throughout the menstrual cycle. In contrast, para-basal cells were estrogen receptorpositive and progesterone receptor-negative in the follicular phase, but estrogen receptor-negative and progesterone receptor -positive and Ki-67 positive in the luteal phase. In cervical precancerous and cancer tissue samples (cervical intraepithelial neoplasia and squamous cervical carcinoma), the expression of estrogen receptors decreased. 31.15% of cervical intraepithelial neoplasia and 11.5% of squamous cervical carcinoma were positive for estrogen receptors. However, the expression of progesterone receptors increased. 29.5% of cervical intraepithelial neoplasia and 49.2% of squamous cervical carcinoma were positive for progesterone receptors. Positive staining for p53 was observed in 15 (24.59%) cases of cervical intraepithelial neoplasia and in 39 (64%) of squamous cervical carcinoma. The expression Ki-67 index in squamous cervical carcinoma cases (47.60%) was significantly higher than of cervical intraepithelial neoplasia cases (30.2%) (p=0.041). Conclusion. The findings of this study suggest that tumor cervical cells evade normal growth control by sex steroid hormones while synchronously abnormal regulatory mechanisms acquire control of the cell cycle.

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