Homophilic synaptic target recognition mediated by immunoglobulin-like cell adhesion molecule Fasciclin III

Development ◽  
1997 ◽  
Vol 124 (20) ◽  
pp. 4143-4152 ◽  
Author(s):  
H. Kose ◽  
D. Rose ◽  
X. Zhu ◽  
A. Chiba
Keyword(s):  
Neural Network ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Growth Cone ◽  
Cell Adhesion Molecule ◽  
Network Formation ◽  
Target Recognition ◽  
Basic Mechanism ◽  
Growth Cones ◽  
Homophilic Interaction

We demonstrate that the cell adhesion molecule Fasciclin III (FAS3) mediates synaptic target recognition through homophilic interaction. FAS3 is expressed by the RP3 motoneuron and its target muscles during synaptic target recognition. The RP3 growth cone can form synapses on muscles that ectopically express FAS3. This mistargeting is dependent on FAS3 expression in the motoneurons. In addition, when the FAS3-negative aCC and SNa motoneuron growth cones ectopically express FAS3, they gain the ability to recognize FAS3-expressing muscles as alternative targets. We propose that homophilic synaptic target recognition serves as a basic mechanism of neural network formation.

Structure of cell–cell adhesion mediated by the Down syndrome cell adhesion molecule

2021 ◽  
Vol 118 (39) ◽  
pp. e2022442118
Author(s):  
Luqiang Guo ◽  
Yichun Wu ◽  
Haishuang Chang ◽  
Ze Zhang ◽  
Hua Tang ◽  
...  
Keyword(s):  
Neural Network ◽  
Down Syndrome ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Neural Development ◽  
Cell Adhesion Molecule ◽  
Network Formation ◽  
Electron Tomography ◽  
Three Dimensional ◽  
Immunoglobulin Superfamily

The Down syndrome cell adhesion molecule (DSCAM) belongs to the immunoglobulin superfamily (IgSF) and plays important roles in neural development. It has a large ectodomain, including 10 Ig-like domains and 6 fibronectin III (FnIII) domains. Previous data have shown that DSCAM can mediate cell adhesion by forming homophilic dimers between cells and contributes to self-avoidance of neurites or neuronal tiling, which is important for neural network formation. However, the organization and assembly of DSCAM at cell adhesion interfaces has not been fully understood. Here we combine electron microscopy and other biophysical methods to characterize the structure of the DSCAM-mediated cell adhesion and generate three-dimensional views of the adhesion interfaces of DSCAM by electron tomography. The results show that mouse DSCAM forms a regular pattern at the adhesion interfaces. The Ig-like domains contribute to both trans homophilic interactions and cis assembly of the pattern, and the FnIII domains are crucial for the cis pattern formation as well as the interaction with the cell membrane. By contrast, no obvious assembly pattern is observed at the adhesion interfaces mediated by mouse DSCAML1 or Drosophila DSCAMs, suggesting the different structural roles and mechanisms of DSCAMs in mediating cell adhesion and neural network formation.

scholarly journals Growth cone localization of neural cell adhesion molecule on central nervous system neurons in vitro.

1986 ◽  
Vol 102 (6) ◽  
pp. 2281-2294 ◽  
Author(s):  
A N van den Pol ◽  
U di Porzio ◽  
U Rutishauser
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Growth Cone ◽  
Colloidal Gold ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Growth Cones ◽  
Neural Cell ◽  
Immunocytochemical Staining ◽  
Neural Cell Adhesion

Ultrastructural analysis of colloidal gold immunocytochemical staining and immunofluorescence microscopy has been used to study the presence of neural cell adhesion molecule (NCAM) on the surface of neuronal growth cones. The studies were carried out with cultures of rat hypothalamic and ventral mesencephalic cells, using morphology and expression of tyrosine hydroxylase, neurofilaments, and glial fibrillary acidic protein as differential markers for neurons and glia. NCAM was found on all plasmalemmal surfaces of neurons including perikarya and neurites. The density of NCAM varied for different neurons growing in the same culture dish, and neurons had at least 25 times more colloidal gold particles on their plasmalemmal membranes than astroglia. Of particular interest in the present study was a strong labeling for NCAM on all parts of neuritic growth cones, including the lamellar and filopodial processes that extend from the tip of the axon. The density of NCAM was similar on different filopodia of the same growth cone. Therefore, in situations where homophilic (NCAM-NCAM) binding might contribute to axon pathfinding, a choice in direction is more likely to reflect differences in the NCAM content of the environment, rather than the distribution of NCAM within a growth cone. On the other hand, the variation in NCAM levels between single neurons in culture was significant and could provide a basis for selective responses of growing neurites.

Testis-Specific Cell Adhesion Molecule, CEACAM6-L, Forms Homophilic Interaction at the Cell Adhesion Site in Vitro

2012 ◽  
Vol 29 (11) ◽  
pp. 786-793 ◽  
Author(s):  
Hitoshi Kurio ◽  
Jae Man Lee ◽  
Takahiro Kusakabe ◽  
Hiroshi Iida
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Specific Cell ◽  
Homophilic Interaction ◽  
Adhesion Site

Function of a Cell Adhesion Molecule, Plexin, in Neuron Network Formation

1997 ◽  
Vol 19 (1) ◽  
pp. 101-105 ◽  
Author(s):  
Hajime Fujisawa ◽  
Kunimasa Ohta ◽  
Toshiki Kameyama ◽  
Yasunori Murakami
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Network Formation ◽  
Neuron Network ◽  
A Cell

scholarly journals The Ig Superfamily Cell Adhesion Molecule, apCAM, Mediates Growth Cone Steering by Substrate–Cytoskeletal Coupling

1998 ◽  
Vol 141 (1) ◽  
pp. 227-240 ◽  
Author(s):  
Daniel M. Suter ◽  
Laura D. Errante ◽  
Victoria Belotserkovsky ◽  
Paul Forscher
Keyword(s):  
Cell Adhesion ◽  
Cell Surface ◽  
Adhesion Molecule ◽  
Growth Cone ◽  
Cell Adhesion Molecule ◽  
Latency Period ◽  
Cytoplasmic Domain ◽  
Cell Surface Receptors ◽  
Vitro Assay ◽  
Surface Receptors

Dynamic cytoskeletal rearrangements are involved in neuronal growth cone motility and guidance. To investigate how cell surface receptors translate guidance cue recognition into these cytoskeletal changes, we developed a novel in vitro assay where beads, coated with antibodies to the immunoglobulin superfamily cell adhesion molecule apCAM or with purified native apCAM, replaced cellular substrates. These beads associated with retrograde F-actin flow, but in contrast to previous studies, were then physically restrained with a microneedle to simulate interactions with noncompliant cellular substrates. After a latency period of ∼10 min, we observed an abrupt increase in bead-restraining tension accompanied by direct extension of the microtubule-rich central domain toward sites of apCAM bead binding. Most importantly, we found that retrograde F-actin flow was attenuated only after restraining tension had increased and only in the bead interaction axis where preferential microtubule extension occurred. These cytoskeletal and structural changes are very similar to those reported for growth cone interactions with physiological targets. Immunolocalization using an antibody against the cytoplasmic domain of apCAM revealed accumulation of the transmembrane isoform of apCAM around bead-binding sites. Our results provide direct evidence for a mechanical continuum from apCAM bead substrates through the peripheral domain to the central cytoplasmic domain. By modulating functional linkage to the underlying actin cytoskeleton, cell surface receptors such as apCAM appear to enable the application of tensioning forces to extracellular substrates, providing a mechanism for transducing retrograde flow into guided growth cone movement.

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