pos-1 encodes a cytoplasmic zinc-finger protein essential for germline specification in C. elegans

Development ◽  
1999 ◽  
Vol 126 (1) ◽  
pp. 1-11 ◽  
Author(s):  
H. Tabara ◽  
R.J. Hill ◽  
C.C. Mello ◽  
J.R. Priess ◽  
Y. Kohara
Keyword(s):  
Germ Cell ◽  
Zinc Finger Protein ◽  
Early Embryogenesis ◽  
Cell Fates ◽  
C Elegans ◽  
Lethal Mutations ◽  
Finger Protein ◽  
Asymmetric Divisions ◽  
Maternal Mrnas ◽  
Novel Protein

Germ cells arise during early C. elegans embryogenesis from an invariant sequence of asymmetric divisions that separate germ cell precursors from somatic precursors. We show that maternal-effect lethal mutations in the gene pos-1 cause germ cell precursors to inappropriately adopt somatic cell fates. During early embryogenesis, pos-1 mRNA and POS-1 protein are present predominantly in the germ precursors. POS-1 is a novel protein with two copies of a CCCH finger motif previously described in the germline proteins PIE-1 and MEX-1 in C. elegans, and in the mammalian TIS11/Nup475/TTP protein. However, mutations in pos-1 cause several defects in the development of the germline blastomeres that are distinct from those caused by mutations in pie-1 or mex-1. The earliest defect detected in pos-1 mutants is the failure to express APX-1 protein from maternally provided apx-1 mRNA, suggesting that POS-1 may have an important role in regulating the expression of maternal mRNAs in germline blastomeres.

scholarly journals Meiosis-specific ZFP541 repressor complex promotes meiotic prophase exit during spermatogenesis

2021 ◽  
Author(s):  
Yuki Horisawa-Takada ◽  
Chisato Kodera ◽  
Kazumasa Takemoto ◽  
Akihiko Sakashita ◽  
Kenichi Horisawa ◽  
...  
Keyword(s):  
Germ Cell ◽  
Meiotic Prophase ◽  
Zinc Finger Protein ◽  
Chromatin Modification ◽  
Biological Processes ◽  
Transcriptional Program ◽  
Transcription Network ◽  
Finger Protein ◽  
Repressor Complex ◽  
Novel Protein

SummaryDuring spermatogenesis, meiosis is accompanied by robust alteration in gene expression and chromatin status. However, it remained elusive how meiotic transcriptional program is established to ensure completion of meiotic prophase. Here, we identified a novel protein complex consisting of germ-cell-specific zinc-finger protein ZFP541 and its interactor KCTD19 as the key transcriptional regulator for meiotic prophase exit. Our genetic study showed that ZFP541 and KCTD19 are co-expressed from pachytene onward and play an essential role in the completion of meiotic prophase program in the testis. Furthermore, our ChIP-seq and transcriptome analyses revealed that ZFP541 binds to and suppresses a broad range of genes whose function is associated with biological processes of transcriptional regulation and covalent chromatin modification. The present study demonstrated that germ-cell specific ZFP541-KCTD19 containing complex promotes meiotic prophase exit in males, and triggers reconstruction of the transcription network and chromatin organization leading to post-meiotic development.

The mab-21 gene of Caenorhabditis elegans encodes a novel protein required for choice of alternate cell fates

Development ◽  
1995 ◽  
Vol 121 (11) ◽  
pp. 3615-3626 ◽  
Author(s):  
K.L. Chow ◽  
D.H. Hall ◽  
S.W. Emmons
Keyword(s):  
Cell Fate ◽  
Hox Genes ◽  
Cell Signalling ◽  
Gene Mutations ◽  
Genetic Modifiers ◽  
Tail Region ◽  
Cell Fates ◽  
C Elegans ◽  
Body Regions ◽  
Novel Protein

The gene mab-21, which encodes a novel protein of 386 amino acids, is required for the choice of alternate cell fates by several cells in the C. elegans male tail. Three cells descended from the ray 6 precursor cell adopt fates of anterior homologs, and a fourth, lineally unrelated hypodermal cell is transformed into a neuroblast. The affected cells lie together in the lateral tail epidermis, suggesting that mab-21 acts as part of a short-range pattern-formation mechanism. Each of the changes in cell fate brought about by mab-21 mutants can be interpreted as a posterior-to-anterior homeotic transformation. mab-21 mutant males and hermaphrodites have additional pleiotropic phenotypes affecting movement, body shape and fecundity, indicating that mab-21 has functions outside the tail region of males. We show that the three known alleles of mab-21 are hypomorphs of a new gene. Mosaic analysis revealed that mab-21 acts cell autonomously to specify the properties of the sensory ray, but non-autonomously in the hypodermal versus neuroblast cell fate choice. Presence of cell signalling in the choice of the neuroblast fate was confirmed by cell ablation experiments. Mutations in mab-21 were shown previously to be genetic modifiers of the effects of HOM-C/Hox gene mutations on ray identity specification. The results presented here support the conclusion that mab-21 acts as part of a mechanism required for correct cell fate choice, possibly involving the function of HOM-C/Hox genes in several body regions.

Patterning of the C. elegans 1 degrees vulval lineage by RAS and Wnt pathways

Development ◽  
2000 ◽  
Vol 127 (23) ◽  
pp. 5047-5058 ◽  
Author(s):  
M. Wang ◽  
P.W. Sternberg
Keyword(s):  
Short Range ◽  
Precursor Cell ◽  
Ras Signaling ◽  
Cell Fates ◽  
C Elegans ◽  
Asymmetric Divisions ◽  
Anchor Cell ◽  
Wnt Pathways ◽  
Vulval Precursor Cell ◽  
Proper Orientation

In C. elegans, the descendants of the 1 degrees vulval precursor cell (VPC) establish a fixed spatial pattern of two different cell fates: E-F-F-E. The two inner granddaughters attach to the somatic gonadal anchor cell (AC) and generate four vulF cells, while the two outer granddaughters produce four vulE progeny. zmp-1::GFP, a molecular marker that distinguishes these two fates, is expressed in vulE cells, but not vulF cells. We demonstrate that a short-range AC signal is required to ensure that the pattern of vulE and vulF fates is properly established. In addition, signaling between the inner and outer 1 degrees VPC descendants, as well as intrinsic polarity of the 1 degrees VPC daughters, is involved in the asymmetric divisions of the 1 degrees VPC daughters and the proper orientation of the outcome. Finally, we provide evidence that RAS signaling is used during this new AC signaling event, while the Wnt receptor LIN-17 appears to mediate signaling between the inner and outer 1 degrees VPC descendants.

scholarly journals LIN-39 and the EGFR/RAS/MAPK pathway regulate C. elegans vulval morphogenesis via the VAB-23 zinc finger protein

Development ◽  
2011 ◽  
Vol 138 (21) ◽  
pp. 4649-4660 ◽  
Author(s):  
M. W. Pellegrino ◽  
S. Farooqui ◽  
E. Frohli ◽  
H. Rehrauer ◽  
S. Kaeser-Pebernard ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Mapk Pathway ◽  
C Elegans ◽  
Finger Protein

scholarly journals Discovery of a novel oocyte-specific Krüppel-associated box domain-containing zinc finger protein required for early embryogenesis in cattle

2017 ◽  
Vol 144 ◽  
pp. 103-112 ◽  
Author(s):  
Jacqelyn M. Hand ◽  
Kun Zhang ◽  
Lei Wang ◽  
Prasanthi P. Koganti ◽  
Kristen Mastrantoni ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Early Embryogenesis ◽  
Finger Protein

scholarly journals Physical and functional interaction between SET1/COMPASS complex component CFP-1 and a Sin3 HDAC complex

2018 ◽  
Author(s):  
F. Beurton ◽  
P. Stempor ◽  
M. Caron ◽  
A. Appert ◽  
Y. Dong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Zinc Finger Protein ◽  
Regulation Of Gene Expression ◽  
Functional Interaction ◽  
Protein Targets ◽  
Coordinate Regulation ◽  
C Elegans ◽  
Finger Protein ◽  
Proteomics Approach ◽  
The Relationship

AbstractThe CFP1 CXXC zinc finger protein targets the SET1/COMPASS complex to non-methylated CpG rich promoters to implement tri-methylation of histone H3 Ly4 (H3K4me3). Although H3K4me3 is widely associated with gene expression, the effects of CFP1 loss depend on chromatin context, so it is important to understand the relationship between CFP1 and other chromatin factors. Using a proteomics approach, we identified an unexpected link betweenC. elegansCFP-1 and a Rpd3/Sin3 histone deacetylase complex. We find that mutants of CFP-1, SIN-3, and the catalytic subunit SET-2/SET1 have similar phenotypes and misregulate common genes. CFP-1 directly binds SIN-3 through a region including the conserved PAH1 domain and recruits SIN-3 and the HDA-1/HDAC subunit to H3K4me3 enriched promoters. Our results reveal a novel role for CFP-1 in mediating interaction between SET1/COMPASS and a Sin3 HDAC complex at promoters and uncover coordinate regulation of gene expression by chromatin complexes having distinct activities.

scholarly journals GLP-1: A novel zinc finger protein required in somatic cells of the gonad for germ cell development

2007 ◽  
Vol 301 (1) ◽  
pp. 106-116 ◽  
Author(s):  
Shanru Li ◽  
Min Min Lu ◽  
Deying Zhou ◽  
Stephen R. Hammes ◽  
Edward E. Morrisey
Keyword(s):  
Germ Cell ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Somatic Cells ◽  
Cell Development ◽  
Germ Cell Development ◽  
Finger Protein
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