scholarly journals In vivo imaging of emerging endocrine cells reveals a requirement for PI3K-regulated motility in pancreatic islet morphogenesis

Development ◽  
2018 ◽  
Vol 145 (3) ◽  
pp. dev158477 ◽  
Author(s):  
Julia Freudenblum ◽  
José A. Iglesias ◽  
Martin Hermann ◽  
Tanja Walsen ◽  
Armin Wilfinger ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
In Vivo Imaging ◽  
Endocrine Cells

scholarly journals A new mode of pancreatic islet innervation revealed by live imaging in zebrafish

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Yu Hsuan Carol Yang ◽  
Koichi Kawakami ◽  
Didier YR Stainier
Keyword(s):  
Neural Crest ◽  
Pancreatic Islets ◽  
Pancreatic Islet ◽  
Endocrine Cells ◽  
Close Contact ◽  
Time Lapse ◽  
Sensory Nerves ◽  
Time Lapse Imaging ◽  
Insight Into

Pancreatic islets are innervated by autonomic and sensory nerves that influence their function. Analyzing the innervation process should provide insight into the nerve-endocrine interactions and their roles in development and disease. Here, using in vivo time-lapse imaging and genetic analyses in zebrafish, we determined the events leading to islet innervation. Comparable neural density in the absence of vasculature indicates that it is dispensable for early pancreatic innervation. Neural crest cells are in close contact with endocrine cells early in development. We find these cells give rise to neurons that extend axons toward the islet as they surprisingly migrate away. Specific ablation of these neurons partly prevents other neurons from migrating away from the islet resulting in diminished innervation. Thus, our studies establish the zebrafish as a model to interrogate mechanisms of organ innervation, and reveal a novel mode of innervation whereby neurons establish connections with their targets before migrating away.

scholarly journals Use of the PET ligand florbetapir for in vivo imaging of pancreatic islet amyloid deposits in hIAPP transgenic mice

Diabetologia ◽  
2018 ◽  
Vol 61 (10) ◽  
pp. 2215-2224 ◽  
Author(s):  
Andrew T. Templin ◽  
Daniel T. Meier ◽  
Joshua R. Willard ◽  
Tami Wolden-Hanson ◽  
Kelly Conway ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Pancreatic Islet ◽  
In Vivo Imaging ◽  
Islet Amyloid ◽  
Amyloid Deposits

scholarly journals Noninvasive in vivo imaging of pancreatic islet cell biology

2008 ◽  
Vol 14 (5) ◽  
pp. 574-578 ◽  
Author(s):  
Stephan Speier ◽  
Daniel Nyqvist ◽  
Over Cabrera ◽  
Jia Yu ◽  
R Damaris Molano ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
In Vivo Imaging ◽  
Cell Biology ◽  
Islet Cell ◽  
Pancreatic Islet Cell

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

In vivo imaging of beta-amyloid load in transgenic rodents with [F-18]FDDNP microPET

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S588-S588
Author(s):  
Vladimir Kepe ◽  
Gregory M Cole ◽  
Jie Liu ◽  
Dorothy G Flood ◽  
Stephen P Trusko ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Beta Amyloid ◽  
Amyloid Load

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon
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