Oscillations and cell development in Dictyostelium discoideum stimulated by folic acid pulses

1977 ◽  
Vol 27 (1) ◽  
pp. 105-114
Author(s):  
B. Wurster ◽  
K. Schubiger
Keyword(s):  
Folic Acid ◽  
Cyclic Amp ◽  
Dictyostelium Discoideum ◽  
Cell Development ◽  
Biochemical Oscillations ◽  
High Concentrations ◽  
Periodic Changes

Folic acid is known to be a chemoattractant of pre-aggregation cells of Dictyostelium discoideum. When supplied in pulses, folic acid induces biochemical oscillations and stimulates the development of pre-aggregation to aggregation-competent amoebae. The continuous supply of folic acid has no stimulatory effect. Folic-acid-induced oscillations are accompanied by periodic changes in the cyclic AMP concentration. Pulses of folic acid applied with rhythms between 7 and 11 min efficiently induce oscillations. In contrast, a rhythm of 2 min neither induces oscillations nor suppresses them. Cells start to oscillate with a rhythm of about 8 min. This inherent rhythm is independent of the inducing rhythm. Oscillating cells are less sensitive to folic acid than pre-oscillating ones. They respond only to high concentrations of folic acid which also interact with the oscillating system.

Cyclic GMP and cyclic AMP changes in response to folic acid pulses during cell development of Dictyostelium discoideum

1979 ◽  
Vol 8 (3) ◽  
pp. 235-242 ◽  
Author(s):  
Bernd Wurster ◽  
Katrin Schubiger ◽  
Philippe Brachet
Keyword(s):  
Folic Acid ◽  
Cyclic Amp ◽  
Dictyostelium Discoideum ◽  
Cyclic Gmp ◽  
Cell Development

scholarly journals Cyclic AMP and folic acid mediated cyclic GMP accumulation in Dictyostelium discoideum

FEBS Letters ◽  
1977 ◽  
Vol 79 (2) ◽  
pp. 331-336 ◽  
Author(s):  
José M. Mato ◽  
Peter J.M. Van Haastert ◽  
Frans A. Krens ◽  
Els H. Rhunsburger ◽  
Fred C.P.M. Dobbe ◽  
...  
Keyword(s):  
Folic Acid ◽  
Cyclic Amp ◽  
Dictyostelium Discoideum ◽  
Cyclic Gmp

scholarly journals Evidence for a messenger function of cyclic GMP during phosphodiesterase induction in Dictyostelium discoideum

1982 ◽  
Vol 152 (1) ◽  
pp. 232-238
Author(s):  
P J van Haastert ◽  
F J Pasveer ◽  
R C van der Meer ◽  
P R van der Heijden ◽  
H van Walsum ◽  
...  
Keyword(s):  
Computer Simulation ◽  
Folic Acid ◽  
Cyclic Amp ◽  
Dictyostelium Discoideum ◽  
Cyclic Gmp ◽  
Close Correlation ◽  
Phosphodiesterase Activity ◽  
Transient Elevation ◽  
Lines Of Evidence ◽  
Stimulation Of

Chemotactic stimulation of vegetative or aggregative Dictyostelium discoideum cells induced a transient elevation of cyclic GMP levels. The addition of chemoattractants to postvegetative cells by pulsing induced phosphodiesterase activity. The following lines of evidence suggest a messenger function for cyclic GMP in the induction of phosphodiesterase: (i) Folic acid and cyclic AMP increased cyclic GMP levels and induced phosphodiesterase activity. (ii) Cyclic AMP induced both cyclic GMP accumulation and phosphodiesterase activity by binding to a rate receptor. (iii) The effects of chemical modification of cyclic AMP or folic acid on cyclic GMP accumulation and phosphodiesterase induction were closely correlated. (iv) A close correlation existed between the increase of cyclic GMP levels and the amount of phosphodiesterase induced, independent of the type of chemoattractant by which this cyclic GMP accumulation was produced. (v) Computer simulation of cyclic GMP binding to intracellular cyclic GMP-binding proteins indicates that half-maximal occupation by cyclic GMP required the same chemoattractant concentration as did half-maximal phosphodiesterase induction.

Folate deaminase and cyclic AMP phosphodiesterase in Dictyostelium discoideum: Their regulation by extracellular cyclic AMP and folic acid

1981 ◽  
Vol 10 (2) ◽  
pp. 79-86 ◽  
Author(s):  
R.L. Bernstein ◽  
C. Rossier ◽  
R. Van Driel ◽  
M. Brunner ◽  
G. Gerisch
Keyword(s):  
Folic Acid ◽  
Cyclic Amp ◽  
Dictyostelium Discoideum ◽  
Cyclic Amp Phosphodiesterase

scholarly journals Chemotaxis to cyclic AMP and folic acid is mediated by different G proteins in Dictyostelium discoideum

1990 ◽  
Vol 96 (4) ◽  
pp. 668-673
Author(s):  
FANJA KESBEKE ◽  
PETER J. M. HAASTERT ◽  
RENÉ J. W. DE WIT ◽  
B. EWA SNAAR-JAGALSKA
Keyword(s):  
Signal Transduction ◽  
Folic Acid ◽  
Cyclic Amp ◽  
G Proteins ◽  
Dictyostelium Discoideum ◽  
Guanylate Cyclase ◽  
Inhibitory Effect ◽  
Sensory Transduction ◽  
Surface Receptors ◽  
Gtpγs Binding

Mutant Frigid A (fgdA) of Dictyostelium discoideum is defective in a functional Ga2 subunit of a G protein and is characterized by a complete blockade of the cyclic AMP-mediated sensory transduction steps, including cyclic AMP relay, chemotaxis and the cyclic GMP response. Folic acid-mediated transmembrane signal transduction was investigated in this mutant; the results show that: (1) cell surface folic acid receptors are present in fgdA mutants. (2) Folic acid induces intracellular responses, including activation of guanylate cyclase and chemotaxis. (3) The inhibitory effect of GTP on folic acid binding to membranes is present. (4) GTPγS binding and highaffinity GTPase are stimulated by folic acid. These data strongly suggest that folic acid receptors are coupled to guanylate cyclase and chemotaxis via a Ga protein that is different from Ga2. The results imply that surface receptors for cyclic AMP and folic acid are coupled to different G proteins.

scholarly journals Regulation of development in Dictyostelium discoideum. IV. Effects of ions on the rate of differentiation and cellular response to cyclic AMP.

1980 ◽  
Vol 87 (3) ◽  
pp. 823-827 ◽  
Author(s):  
F T Marin ◽  
F G Rothman
Keyword(s):  
Cyclic Amp ◽  
Dictyostelium Discoideum ◽  
Early Development ◽  
Maximum Rate ◽  
Cellular Response ◽  
Developmental Regulation ◽  
Intrinsic Rate ◽  
Rate Of Development ◽  
High Concentrations ◽  
Early Developmental

The effects of ionic environment on both the intrinsic rate of differentiation and the response to exogenous cyclic AMP in Dictyostelium discoideum have been examined. K+ specifically inhibits the rate of early development when present at concentrations > 15 mM. Na+ does not inhibit at concentrations up to 25 mM, and can partially overcome K+ inhibition. The maximum rate of development also depends upon the presence of adequate levels of extracellular Ca++. The effect of exogenous cyclic AMP on the rate of development is inhibited by the absence of Ca++, and/or the presence of high concentrations of K+. Under optimal ionic conditions, the only effect of exogenous cyclic AMP on early developments of K+. Under optimal ionic conditions, the only effect of exogenous cyclic AMP on early development is a specific inhibition. The implications of these results for current models of early developmental regulation are discussed.

Challenge with high concentrations of cyclic AMP induces transient changes in the cytosolic free calcium concentration in Dictyostelium discoideum

1994 ◽  
Vol 107 (8) ◽  
pp. 2107-2115 ◽  
Author(s):  
C. Schlatterer ◽  
F. Gollnick ◽  
E. Schmidt ◽  
R. Meyer ◽  
G. Knoll
Keyword(s):  
Cyclic Amp ◽  
Dictyostelium Discoideum ◽  
Free Calcium ◽  
Vegetative Cells ◽  
Phosphoinositide Signaling ◽  
Competent Cells ◽  
Dependent Protein ◽  
Free Calcium Concentration ◽  
High Concentrations ◽  
Camp Receptor

Dictyostelium discoideum cells use cyclic AMP (cAMP) for chemotactic signaling as well as for differentiation. The precise regulation of the cytosolic Ca2+ concentration ([Ca2+]i) seems to play a key role for both processes. We performed single cell measurements of [Ca2+]i in amoebae that were starved in suspension for various times and scrape-loaded with the Ca2+ indicator fura-2. Stimulation of cells with cAMP at the concentration required to induce gene expression (> or = 100 microM) elicited a global transient increase in [Ca2+]i that depended on the presence of external Ca2+. Both vegetative and aggregation-competent cells displayed a rise in [Ca2+]i, with aggregation-competent cells responding more often than vegetative cells. Basal [Ca2+]i in the presence of Ca2+ was high in vegetative cells and declined during development; the cAMP-induced rise in [Ca2+]i was higher and lasted longer in vegetative cells than in aggregative cells. The addition of 2′-deoxy-cAMP, which binds to the cAMP receptor, induced an increase in [Ca2+]i, whereas the membrane-permeant analogue 8-bromo-cAMP that has a low affinity for the receptor but activates cAMP-dependent protein kinase had no effect. This indicates that the change in [Ca2+]i is mediated by the cell surface cAMP receptor. Since HC85 mutant cells, which lack the G alpha 2 subunit of the G-protein that couples the receptor to phospholipase C, also responded to stimulation with cAMP, the Ca2+ influx does not seem to be triggered by the phosphoinositide signaling cascade.(ABSTRACT TRUNCATED AT 250 WORDS)

In Vitro Effects of Ethanol on Rabbit Platelet Aggregation, Secretion of Granule Contents, and Cyclic AMP Levels in the Presence of Prostacyclin

1989 ◽  
Vol 61 (02) ◽  
pp. 254-258 ◽  
Author(s):  
Margaret L Rand ◽  
Peter L Gross ◽  
Donna M Jakowec ◽  
Marian A Packham ◽  
J Fraser Mustard
Keyword(s):  
Cyclic Amp ◽  
Inhibitory Effect ◽  
Rabbit Platelet ◽  
Inhibitory Effects ◽  
Low Concentrations ◽  
Rabbit Platelets ◽  
High Concentrations ◽  
In Vitro Effects ◽  
Aggregation Of Platelets

SummaryEthanol, at physiologically tolerable concentrations, inhibits platelet responses to low concentrations of collagen or thrombin, but does not inhibit responses of washed rabbit platelets stimulated with high concentrations of ADP, collagen, or thrombin. However, when platelet responses to high concentrations of collagen or thrombin had been partially inhibited by prostacyclin (PGI2), ethanol had additional inhibitory effects on aggregation and secretion. These effects were also observed with aspirin- treated platelets stimulated with thrombin. Ethanol had no further inhibitory effect on aggregation of platelets stimulated with ADP, or the combination of ADP and epinephrine. Thus, the inhibitory effects of ethanol on platelet responses in the presence of PGI2 were very similar to its inhibitory effects in the absence of PGI2, when platelets were stimulated with lower concentrations of collagen or thrombin. Ethanol did not appear to exert its inhibitory effects by increasing cyclic AMP above basal levels and the additional inhibitory effects of ethanol in the presence of PGI2 did not appear to be brought about by further increases in platelet cyclic AMP levels.

scholarly journals A cytosolic cyclic AMP-dependent protein kinase in Dictyostelium discoideum. I. Properties.

1984 ◽  
Vol 259 (1) ◽  
pp. 654-661 ◽  
Author(s):  
I H Majerfeld ◽  
B H Leichtling ◽  
J A Meligeni ◽  
E Spitz ◽  
H V Rickenberg
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Dictyostelium Discoideum ◽  
Dependent Protein Kinase ◽  
Dependent Protein
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