scholarly journals Failure of Vitamin E to Extend the Life Span of a Human Diploid Cell Line in Culture

1977 ◽  
Vol 2 (3) ◽  
pp. 219-227 ◽  
Author(s):  
Hiroshi Sakagami ◽  
Masa-atsu Yamada
Keyword(s):  
Vitamin E ◽  
Life Span ◽  
Cell Line ◽  
Diploid Cell ◽  
Human Diploid Cell ◽  
Diploid Cell Line

Relationship Between Freezing Rate, Ultrastructure and Recovery in a Human Diploid Cell Line

1974 ◽  
Vol 15 (2) ◽  
pp. 419-427
Author(s):  
D. J. BEADLE ◽  
L. W. HARRIS
Keyword(s):  
Cell Line ◽  
Structural Damage ◽  
Diploid Cell ◽  
Freezing Rate ◽  
Human Diploid Cell ◽  
Simple Correlation ◽  
Thaw Cycle ◽  
Freeze Thaw Cycle ◽  
Diploid Cell Line ◽  
The Relationship

The relationship between freezing rate, ultrastructure and recovery in a human diploid cell line has been studied by freezing cells at rates that are known to give high and low recoveries and examining them immediately after thawing. Some correlation was found between structural damage and recovery. The main types of damage observed were loss of cytoplasm and nucleoplasm, indicating disruption of cellular membranes, and swelling of subcellular organelles due to osmotic changes during the freeze-thaw cycle. No simple correlation was found between freezing rate and structural damage. In the absence of a cryoprotectant both rapid and slow freezing produced similar types and amounts of damage resulting in low recovery. In the presence of 10% dimethylsulphoxide, however, slowly frozen cells showed few signs of damage and recovery was high. DMSO had no such protective effect on rapidly frozen cells.

Changes of enzymic activities in human diploid cell line WI-38 at various passages

1970 ◽  
Vol 61 (2-3) ◽  
pp. 357-364 ◽  
Author(s):  
K.-M. Wang ◽  
N.R. Rose ◽  
E.A. Bartholomew ◽  
M. Balzer ◽  
K. Berde ◽  
...  
Keyword(s):  
Cell Line ◽  
Diploid Cell ◽  
Human Diploid Cell ◽  
Diploid Cell Line

Characterization of a new human diploid cell line—IMR-91

In Vitro ◽  
1983 ◽  
Vol 19 (10) ◽  
pp. 797-804 ◽  
Author(s):  
Warren W. Nichols ◽  
Vincent J. Cristofalo ◽  
Lorraine H. Toji ◽  
Arthur E. Greene ◽  
Margaret M. Aronson ◽  
...  
Keyword(s):  
Cell Line ◽  
Diploid Cell ◽  
Human Diploid Cell ◽  
Diploid Cell Line

scholarly journals Comparative study of the immunogenicity in mice and monkeys of an inactivated CA16 vaccine made from a human diploid cell line

2014 ◽  
Vol 10 (5) ◽  
pp. 1266-1273 ◽  
Author(s):  
Erxia Yang ◽  
Chen Cheng ◽  
Ying Zhang ◽  
Jingjing Wang ◽  
Yanchun Che ◽  
...  
Keyword(s):  
Comparative Study ◽  
Cell Line ◽  
Diploid Cell ◽  
Human Diploid Cell ◽  
Diploid Cell Line

Replication of cytomegalovirus in human epitheloid diploid cell line

1976 ◽  
Vol 52 (4) ◽  
pp. 283-296 ◽  
Author(s):  
V. Vonka ◽  
E. Anisimov� ◽  
M. Macek
Keyword(s):  
Cell Line ◽  
Diploid Cell ◽  
Diploid Cell Line

scholarly journals A placental diploid cell line is not essential for ongoing trisomy 13 or 18 pregnancies

2001 ◽  
Vol 9 (4) ◽  
pp. 286-290 ◽  
Author(s):  
GHeleen Schuring-Blom ◽  
Kees Boer ◽  
NicoJ Leschot
Keyword(s):  
Cell Line ◽  
Diploid Cell ◽  
Trisomy 13 ◽  
Diploid Cell Line

Cytochemical Localization of Acid Phosphatase in WI-38 Cells Observed by HighVoltage Electron Microscopy

1975 ◽  
Vol 33 ◽  
pp. 446-447
Author(s):  
John J. Wolosewick ◽  
Keith R. Porter
Keyword(s):  
Acid Phosphatase ◽  
Cultured Cells ◽  
Diploid Cell ◽  
Human Diploid Cell ◽  
Sodium Cacodylate ◽  
Routine Procedure ◽  
Cytochemical Localization ◽  
Energy Beam ◽  
Voltage Electron ◽  
Diploid Cell Line

One of the major problems in the interpretation of high voltage electron micrographs of whole cultured cells results from the superimposition of membranes, filaments, ribosomes, etc., in the final 2-dimensional image. The gains provided by the greater penetrability and resolution of the higher energy beam are to some extent lost in the confusion introduced by the overlap of structures. The problem is overcome in part by stereomicroscopy and this becomes the routine procedure for thick specimens. The effectiveness of this is greatly improved, however, if the object or organelle of special interest can be selectively stained. Toward this end we have explored the use of cytochemical techniques which identify the distribution of certain intracellular enzymes. The results will be illustrated with procedures for acid phosphatase.The “aging” human diploid cell line WI-38 (passages 25-30) was cultured on sterilized, formvar-coated gold grids lightly shadowed with carbon. The cells were fixed for 1 hr. at 0-4°C in 2% glutaraldehyde in 0.1M sodium cacodylate (pH 7.2) plus 0.1M sucrose.

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