scholarly journals Identification and Characterization of an Outer Membrane Receptor Gene in Acinetobacter baumannii Required for Utilization of Desferricoprogen, Rhodotorulic Acid, and Desferrioxamine B as Xenosiderophores

2012 ◽  
Vol 35 (5) ◽  
pp. 753-760 ◽  
Author(s):  
Tatsuya Funahashi ◽  
Tomotaka Tanabe ◽  
Kazutoshi Mihara ◽  
Katsushiro Miyamoto ◽  
Hiroshi Tsujibo ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Outer Membrane ◽  
Receptor Gene ◽  
Membrane Receptor ◽  
Outer Membrane Receptor ◽  
Rhodotorulic Acid ◽  
Desferrioxamine B ◽  
Identification And Characterization

scholarly journals Characterization of a Gene Encoding the Outer Membrane Receptor for Ferric Enterobactin in Aeromonas hydrophila ATCC 7966T

2013 ◽  
Vol 77 (2) ◽  
pp. 353-360 ◽  
Author(s):  
Tatsuya FUNAHASHI ◽  
Tomotaka TANABE ◽  
Katsushiro MIYAMOTO ◽  
Hiroshi TSUJIBO ◽  
Jun MAKI ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Aeromonas Hydrophila ◽  
Membrane Receptor ◽  
Outer Membrane Receptor ◽  
Gene Encoding

scholarly journals Identification and characterization of an iron-regulated gene, chtA, required for the utilization of the xenosiderophores aerobactin, rhizobactin 1021 and schizokinen by Pseudomonas aeruginosa

Microbiology ◽  
2006 ◽  
Vol 152 (4) ◽  
pp. 945-954 ◽  
Author(s):  
Páraic Ó Cuív ◽  
Paul Clarke ◽  
Michael O'Connell
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Outer Membrane ◽  
Membrane Receptor ◽  
Outer Membrane Receptor ◽  
Significant Similarity ◽  
Hydroxamate Siderophores ◽  
Insertional Inactivation ◽  
Titration Assay

Pseudomonas aeruginosa utilizes several xenosiderophores under conditions of iron limitation, including the citrate hydroxamate siderophore aerobactin. Analysis of the P. aeruginosa genome sequence revealed the presence of two genes, chtA (PA4675) and PA1365, encoding proteins displaying significant similarity to the aerobactin outer-membrane receptor, IutA, of Escherichia coli. The chtA and PA1365 genes were mutated by insertional inactivation and it was demonstrated that ChtA is the outer-membrane receptor for aerobactin. ChtA also mediated the utilization of rhizobactin 1021 and schizokinen, which are structurally similar to aerobactin. In contrast to the utilization of other xenosiderophores by P. aeruginosa, there was no apparent redundancy in the utilization of aerobactin, rhizobactin 1021 and schizokinen. The utilization of citrate hydroxamate siderophores by P. aeruginosa was demonstrated to be TonB1 dependent. A Fur box was identified in the region directly upstream of chtA and it was demonstrated by the in vivo Fur titration assay that this region is capable of binding Fur and accordingly that expression of chtA is iron regulated. The PA1365 mutant was unaffected in the utilization of citrate hydroxamate siderophores.

Identification of siderophores ofPseudomonas stutzeri

2006 ◽  
Vol 52 (12) ◽  
pp. 1164-1176 ◽  
Author(s):  
Anna M Zawadzka ◽  
Frederik P.J Vandecasteele ◽  
Ronald L Crawford ◽  
Andrzej J Paszczynski
Keyword(s):  
Outer Membrane ◽  
Receptor Gene ◽  
Pseudomonas Stutzeri ◽  
Membrane Receptor ◽  
Outer Membrane Receptor ◽  
Colony Hybridization ◽  
Normal Spectrum ◽  
Pseudomonas Balearica

We have identified two types of siderophores produced by Pseudomonas, one of which has never before been found in the genus. Twelve strains of Pseudomonas stutzeri belonging to genomovars 1, 2, 3, 4, 5, and 9 produced proferrioxamines, the hydroxamate-type siderophores. Pseudomonas stutzeri JM 300 (genomovar 7) and DSM 50238 (genomovar 8) and Pseudomonas balearica DSM 6082 produced amonabactins, catecholate-type siderophores. The major proferrioxamines detected were the cyclic proferrioxamines E and D2. Pseudomonas stutzeri KC also produced cyclic (X1and X2) and linear (G1and G2a-c) proferrioxamines. Our data indicate that the catecholate-type siderophores belong to amonabactins P 750, P 693, T 789, and T 732. A mutant of P. stutzeri KC (strain CTN1) that no longer produced the secondary siderophore pyridine-2,6-dithiocarboxylic acid continued to produce all other siderophores in its normal spectrum. Siderophore profiles suggest that strain KC (genomovar 9) belongs to the proferrioxamine-producing P. stuzeri. Moreover, a putative ferrioxamine outer membrane receptor gene foxA was identified in strain KC, and colony hybridization showed the presence of homologous receptor genes in all P. stutzeri and P. balearica strains tested.Key words: siderophore, Pseudomonas stutzeri, ferrioxamine, amonabactin.

Characterization of the stability functions and inverted repeat structure X3 of the IncFI plasmid ColV2-K94

1986 ◽  
Vol 32 (10) ◽  
pp. 806-813
Author(s):  
Peter C. Weber ◽  
Sunil Palchaudhuri
Keyword(s):  
Outer Membrane ◽  
Inverted Repeat ◽  
Iron Uptake ◽  
Membrane Receptor ◽  
Outer Membrane Receptor ◽  
Plasmid Maintenance ◽  
Stability Functions ◽  
Repeat Structure ◽  
The Stability

A region of the IncFI plasmid ColV2-K94 which showed homology to the sop partitioning genes of F was cloned and characterized in an attempt to study the stability functions of this element. The sop region contained the incD incompatibility determinant common to many IncFI plasmids, but could not confer on ColV2-K94 miniplasmids the same stable inheritance found in the intact ColV2-K94; thus, other functions appear to be required for efficient plasmid maintenance. Adjacent to the area of sop homology was the X3 region, which was found to contain three inverted IS1-like sequences. The X3 region of ColV2-K94 was similar in organization to the aerobactin iron uptake region of ColV3-K30, but ColV2-K94 lacked the ability to synthesize either the aerobactin siderophore or its outer membrane receptor.

Characterization of the cloacin DF13/aerobactin outer membrane receptor protein of Escherichia coli (pFS8)

1985 ◽  
Vol 51 (5-6) ◽  
pp. 553-554
Author(s):  
Willy J. A. Krone ◽  
Freek Stegehuis ◽  
Frits K. de Graaf ◽  
Bauke Oudega
Keyword(s):  
Escherichia Coli ◽  
Outer Membrane ◽  
Membrane Receptor ◽  
Receptor Protein ◽  
Outer Membrane Receptor
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