The Effects of Bioactive Compounds from Blueberry and Blackcurrant Powder on Oat Bran Pastes: Enhancing In Vitro Antioxidant Activity and Reducing Reactive Oxygen Species in Lipopolysaccharide-Stimulated Raw264.7 Macrophages

In this study, blueberry and blackcurrant powder were chosen as the phenolic-rich enrichments for oat bran. A Rapid Visco Analyser was used to form blueberry and blackcurrant enriched oat pastes. An in vitro digestion process evaluated the changes of phenolic compounds and the in vitro antioxidant potential of extracts of pastes. The anthocyanidin profiles in the extracts were characterised by the pH differential method. The results showed that blueberry and blackcurrant powder significantly increased the content of phenolic compounds and the in vitro antioxidant capacity of pastes, while the total flavonoid content decreased after digestion compared to the undigested samples. Strong correlations between these bioactive compounds and antioxidant values were observed. Lipopolysaccharide-stimulated RAW264.7 macrophages were used to investigate the intracellular antioxidant activity of the extracts from the digested oat bran paste with 25% enrichment of blueberry or blackcurrant powder. The results indicated that the extracts of digested pastes prevented the macrophages from experiencing lipopolysaccharide (LPS)-stimulated intracellular reactive oxygen species accumulation, mainly by the Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2) signalling pathway. These findings suggest that the bioactive ingredients from blueberry and blackcurrant powder enhanced the in vitro and intracellular antioxidant capacity of oat bran pastes, and these enriched pastes have the potential to be utilised in the development of the functional foods.

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Phenolic Compounds Present in Medicinal Mushroom Extracts Generate Reactive Oxygen Species in Human Cells In Vitro

International Journal of Medicinal Mushrooms ◽

10.1615/intjmedmushr.v10.i1.20 ◽

2008 ◽

Vol 10 (1) ◽

pp. 1-13 ◽

Cited By ~ 19

Author(s):

Song Wei ◽

Johannes P. F. G. Helsper ◽

L. J. L. D. Van Griensven

Keyword(s):

Reactive Oxygen Species ◽

Phenolic Compounds ◽

Reactive Oxygen ◽

Human Cells ◽

Oxygen Species ◽

Medicinal Mushroom ◽

Mushroom Extracts

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In vitro Antitumour Activity of Tomato-Extracted Carotenoids on Human Colorectal Carcinoma

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4329982 ◽

2015 ◽

Vol 43 (2) ◽

pp. 293-301 ◽

Cited By ~ 2

Author(s):

Diana CENARIU ◽

Eva FISCHER-FODOR ◽

Piroska VIRAG ◽

Corina TATOMIR ◽

Mihai CENARIU ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Colon Cancer ◽

Antioxidant Activity ◽

Cancer Cell ◽

Antitumour Activity ◽

Reactive Oxygen ◽

Signalling Pathway ◽

Oxygen Species ◽

Tnf Receptors

The aim of this research was to establish whether all-trans lycopene extracted from fresh and frozen tomatoes is able to inhibit the in vitro proliferation of colon cancer cells, to trigger apoptosis by reactive oxygen species modulation and to reveal its influence on NF-kÎ² signalling, through the p65 transcription factor and expression of two TNF receptors: GITR and CD27. The carotenoid extracts containing all-trans lycopene were obtained from fresh (E1) and frozen/thawed (E2) tomatoes (Lycopersicon esculentum Mill.), hybrid â€˜Menhirâ€™ F1. DLD-1 and HT-29 human colon adenocarcinoma cell lines were co-cultivated with the two extracts and cytotoxicity, apoptosis, antioxidant activity, reactive oxygen species as well as modulation of NF-kÎ² signalling pathway were assessed. Tomato extracts E1 and E2 were able to inhibit colon cancer cell growth in vitro. E2 contained a higher proportion of all-trans lycopene and displayed superior cytotoxicity and a better apoptosis inducing capacity. The two extracts proved antioxidant activity against DPPH radicals and were able to scavenge the reactive oxygen species in the treated tumour cells. This study also showed that lycopene acts mainly through p65 protein and moderately by TNF receptors GITR and CD27 to deactivate the NF-kÎ² signalling pathway involved in cancer cell proliferation.

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Antioxidant Properties of Galantamine Hydrobromide

Zeitschrift für Naturforschung C ◽

10.1515/znc-2003-5-613 ◽

2003 ◽

Vol 58 (5-6) ◽

pp. 361-365 ◽

Cited By ~ 28

Author(s):

M. Traykova ◽

T. Traykov ◽

V. Hadjimitova ◽

N. Bojadgieva

Keyword(s):

Reactive Oxygen Species ◽

Antioxidant Activity ◽

Antioxidant Properties ◽

Reactive Oxygen ◽

Oxygen Species ◽

Quaternary Nitrogen ◽

Scavenging Effect ◽

Brain Degeneration ◽

Antioxidant Effects

Abstract The antioxidant properties of galantamine hydrobromide ((4α,6β)-4a,5,9,10,11,12-hexahydro- 3-methoxy-11-methyl-6H-benzofuro[3a,3,2-ef][2]benzazepin-6-ol hydrobromide) were studied in vitro, using luminol-dependent chemiluminescence and spectrophotometry. It was found that this compound was a scavenger of reactive oxygen species (ROS). By comparing the antioxidant effects of galantamine ((4α,6β)-4a,5,9,10,11,12-hexahydro-3-methoxy-11-methyl- 6H-benzofuro[3a,3,2-ef][2]benzazepin-6-ol), galantamine hydrobromide, narwedine (4a, 5,9,10,11,12-hexahydro-3-methoxy-11-methyl-6H-benzofuro[3a,3,2-ef][2]benzazepin-6-one), and narwedine hydrobromide it was found that the antioxidant activity depended on the enolic OH group in the molecule. The presence of a quaternary nitrogen in the compound increased the strength of the scavenging effect. It is proposed that the antioxidant properties observed in vitro may contribute to the therapeutical effect of galantamine hydrobromide on patients with brain degeneration.

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In Vitro Evaluation of Antioxidant Potential of the Invasive Seagrass Halophila stipulacea

Marine Drugs ◽

10.3390/md19010037 ◽

2021 ◽

Vol 19 (1) ◽

pp. 37

Author(s):

Clementina Sansone ◽

Christian Galasso ◽

Marco Lo Martire ◽

Tomás Vega Fernández ◽

Luigi Musco ◽

...

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Antioxidant Activity ◽

Cell Line ◽

Reactive Oxygen ◽

Indigenous Species ◽

Great Activity ◽

Oxygen Species ◽

Halophila Stipulacea

Marine organisms with fast growth rates and great biological adaptive capacity might have biotechnological interests, since ecological competitiveness might rely on enhanced physiological or biochemical processes’ capability promoting protection, defense, or repair intracellular damages. The invasive seagrass Halophila stipulacea, a non-indigenous species widespread in the Mediterranean Sea, belongs to this category. This is the premise to investigate the biotechnological interest of this species. In this study, we investigated the antioxidant activity in vitro, both in scavenging reactive oxygen species and in repairing damages from oxidative stress on the fibroblast human cell line WI-38. Together with the biochemical analysis, the antioxidant activity was characterized by the study of the expression of oxidative stress gene in WI-38 cells in presence or absence of the H. stipulacea extract. Concomitantly, the pigment pool of the extracts, as well as their macromolecular composition was characterized. This study was done separately on mature and young leaves. Results indicated that mature leaves exerted a great activity in scavenging reactive oxygen species and repairing damages from oxidative stress in the WI-38 cell line. This activity was paralleled to an enhanced carotenoids content in the mature leaf extracts and a higher carbohydrate contribution to organic matter. Our results suggest a potential of the old leaves of H. stipulacea as oxidative stress damage protecting or repair agents in fibroblast cell lines. This study paves the way to transmute the invasive H. stipulacea environmental threat in goods for human health.

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Synergistic antioxidant activity of size controllable chitosan-templated Prussian blue nanoparticle

Nanomedicine ◽

10.2217/nnm-2019-0223 ◽

2019 ◽

Vol 14 (19) ◽

pp. 2567-2578 ◽

Cited By ~ 4

Author(s):

Hyeryeon Oh ◽

Jin Sil Lee ◽

Daekyung Sung ◽

Jin Hyung Lee ◽

Sang Hyun Moh ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Antioxidant Activity ◽

Prussian Blue ◽

Reactive Oxygen Species Generation ◽

Reactive Oxygen ◽

Water Soluble ◽

Oxygen Species ◽

Antioxidant Agents

Aim: Prussian blue nanoparticles (PB NPs) have been reported as excellent antioxidant agents owing to their ability to scavenge reactive oxygen species. However, their poor stability in vivo limits their use in biomedical applications. Materials & methods: In this study, we developed chitosan-templated PB NPs using water-soluble chitosan samples with molecular weights ranging from 3 to 100 kDa, which stabilized the PB NPs and improved their antioxidant activity. Results & conclusion: The chitosan-templated PB NPs coordinated with the optimal chitosan molecular weight had uniform sphere-like particles, improved stability and effective scavenging activity of in vitro reactive oxygen species generation in murine fibroblast cells stimulated by oxidative stress agents without any cytotoxicity, implying that they could be promising antioxidant agents.

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Antioxidant Activity of Hydrogen Nanobubbles in Water with Different Reactive Oxygen Species both in Vivo and in Vitro

Langmuir ◽

10.1021/acs.langmuir.8b02440 ◽

2018 ◽

Vol 34 (39) ◽

pp. 11878-11885 ◽

Cited By ~ 1

Author(s):

Shu Liu ◽

Seiichi Oshita ◽

Dang Quoc Thuyet ◽

Masanao Saito ◽

Takahiko Yoshimoto

Keyword(s):

Reactive Oxygen Species ◽

Antioxidant Activity ◽

Reactive Oxygen ◽

Oxygen Species

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Structure Effect on Antioxidant Activity of Catecholamines toward Singlet Oxygen and Other Reactive Oxygen Species in vitro

Journal of Clinical Biochemistry and Nutrition ◽

10.3164/jcbn.09-112 ◽

2010 ◽

Vol 47 (3) ◽

pp. 181-190 ◽

Cited By ~ 18

Author(s):

Takako Shimizu ◽

Yuji Nakanishi ◽

Meiko Nakahara ◽

Naoki Wada ◽

Yoshihiko Moro-oka ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Antioxidant Activity ◽

Singlet Oxygen ◽

Reactive Oxygen ◽

Structure Effect ◽

Oxygen Species

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Bioactive compounds and in vitro antioxidant activity of pulps from fruits from the Brazilian atlantic forest

Acta Scientiarum Technology ◽

10.4025/actascitechnol.v42i1.44503 ◽

2019 ◽

Vol 42 ◽

pp. e44503 ◽

Cited By ~ 1

Author(s):

Ana Carolina Leme Castelucci ◽

Paula Porrelli Moreira da Silva ◽

Marta Helena Fillet Spoto

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Phenolic Compounds ◽

Antioxidant Capacity ◽

Bioactive Compounds ◽

Multivariate Statistical ◽

The Family ◽

The One ◽

Free Radical Activity

The fruits belonging to the family Myrtaceae are known sources of compounds with functional characteristics. Nevertheless, the studies are focused only on some species of this family. In this sense, we aimed to quantify the bioactive compounds present in the pulps of cambuci, feijoa, uvaia and grumixama; to evaluate the in vitro antioxidant capacity of each one and to correlate the contribution of these bioactive compounds with the antioxidant activity of each fruit pulp. For this, the compounds ascorbic acid, phenolic compounds, carotenoids, and flavonoids were quantified for the pulps of cambuci, feijoa, uvaia, and grumixama, as well as the in vitro antioxidant capacity by the methods DPPH and ABTS. The results were evaluated by multivariate statistical techniques. The pulps present good antioxidant potential, the one from cambuci presented the highest values for antioxidant activity given by the method DPPH (61.86 µg of Trolox g-1), that from uvaia was prominent by the presence of ascorbic acid (85.40 ascorbic acid 100 g-1) and that from feijoa, by the flavonoid contents (62.45 mg quercetin g-1) and phenolic compounds (10.21 mg gallic acid equivalent g–1). A correlation was observed between pulp antioxidant capacity and the contents of ascorbic acid and carotenoids; on the other hand, the phenolic compounds and flavonoids little contributed for the anti-free radical activity of the methods DPPH and ABTS.

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