Effect of Protein Intake on IGF-I System Responses to a 7-Day Exercise-Induced Negative Energy Balance

Severe negative energy balance (NEB) in the postpartum period of dairy cows may be associated with declining fertility but the mechanisms by which nutrition influences reproduction are complex, poorly understood and confounded by lactation. Hence, both chronic and acute nutritional restriction of beef heifers have been used as models to examine the effects of NEB on ovarian and endocrine responses in the absence of lactation. Plasma IGF-I concentrations gradually decreased until the onset of anoestrus (Stagg et al., 1999) but concentrations may be confounded with stage of the oestrous cycle, especially around ovulation (Mackey et al., 2000). Therefore, the aim of this study was to examine the effect of nutritional restriction on periovulatory oestradiol (E2) and IGF-I concentrations.

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Nutritional effects on resumption of ovarian cyclicity and conception rate in postpartum dairy cows

BSAP Occasional Publication ◽

10.1017/s0263967x00033644 ◽

2001 ◽

Vol 26 (1) ◽

pp. 133-145 ◽

Cited By ~ 31

Author(s):

W.R. Butler

Keyword(s):

Energy Balance ◽

Dairy Cows ◽

Milk Production ◽

Dietary Protein ◽

Negative Energy ◽

Negative Energy Balance ◽

Breeding Period ◽

Plasma Urea ◽

Igf I ◽

High Dietary Protein

AbstractIncreased genetic potential for milk production has been associated with a decline in fertility of lactating cows. Following parturition the nutritional requirements increase rapidly with milk production and result in negative energy balance (NEBAL). NEBAL delays the time of first ovulation thereby affecting ovarian cycles before and during the subsequent breeding period The effects of NEBAL on reinitiation of ovulation are manifested through inhibition of LH pulse frequency and low levels of glucose, insulin and IGF-I in blood that collectively restrain oestrogen production by dominant follicles. Upregulation of LH pulses and peripheral IGF-I in association with the NEBAL nadir increases the likelihood that emerging dominant follicles will ovulate. The legacy of NEBAL is reduced fertility after insemination in conjunction with reduced serum progesterone concentrations. Diets high in crude protein support high milk yield, but may be detrimental to reproductive performance. Depending upon protein quantity and composition, serum concentrations of progesterone may be lower and the uterine luminal environment is altered. High protein intake is correlated with plasma urea concentrations that are inversely related to uterine pH and fertility. The direct effects of high dietary protein and plasma urea on embryo quality and development in cattle are inconsistent. In conclusion, the poor fertility of high producing dairy cows reflects the combined effects of a uterine environment that is dependent on progesterone, but has been rendered suboptimal for embryo development by antecedent effects of negative energy balance and may be further compromised by the effects of urea resultingfrom intake of high dietary protein.

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Plasma adrenal, gonadal, and conjugated steroids following long-term exercise-induced negative energy balance in identical twins

Metabolism ◽

10.1016/s0026-0495(99)90125-7 ◽

1999 ◽

Vol 48 (9) ◽

pp. 1120-1127 ◽

Cited By ~ 34

Author(s):

Janet Pritchard ◽

Jean-Pierre Després ◽

Jacques Gagnon ◽

Adnré Tchernof ◽

Adnré Nadeau ◽

...

Keyword(s):

Energy Balance ◽

Negative Energy ◽

Identical Twins ◽

Negative Energy Balance ◽

Exercise Induced ◽

Conjugated Steroids

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Insulin restores GH responsiveness during lactation-induced negative energy balance in dairy cattle: effects on expression of IGF-I and GH receptor 1A

Journal of Endocrinology ◽

10.1677/joe.0.1760205 ◽

2003 ◽

Vol 176 (2) ◽

pp. 205-217 ◽

Cited By ~ 172

Author(s):

ST Butler ◽

AL Marr ◽

SH Pelton ◽

RP Radcliff ◽

MC Lucy ◽

...

Keyword(s):

Adipose Tissue ◽

Blood Glucose ◽

Energy Balance ◽

Dairy Cattle ◽

Negative Energy ◽

Negative Energy Balance ◽

Mrna Levels ◽

Hepatic Expression ◽

Gh Receptor ◽

Igf I

Early lactation in dairy cattle is a period of severe negative energy balance (NEB) characterized by reduced blood glucose and insulin concentrations and elevated blood GH concentrations. The liver is refractory to GH during NEB and this uncoupling of the GH-IGF axis results in diminished plasma concentrations of IGF-I. Our objectives were to examine the effects of insulin administration during the immediate postpartum period on plasma IGF-I and GH concentrations and to examine the hepatic expression of total GH receptors (all GH receptor transcripts), GH receptor 1A (GHR 1A) and IGF-I. In addition, we examined adipose tissue for total GH receptor and IGF-I mRNA levels to establish the effects of chronic hyperinsulinemia on an insulin-responsive peripheral tissue. Holstein cows (n=14) were subjected to either a hyperinsulinemic-euglycemic clamp (insulin; INS) or saline infusion (control; CTL) for 96 h starting on day 10 postpartum. Insulin was infused i.v. (1 micro g/kg body weight per h), blood samples were collected hourly, and euglycemia was maintained by infusion of glucose. Insulin concentrations during the infusions were increased 8-fold in INS compared with CTL cows (2.33+/-0.14 vs 0.27+/-0.14 ng/ml (S.E.M.); P<0.001) while blood glucose concentrations were not different between treatments (45.3+/-2.2 vs 42.5+/-2.2 mg/dl; P>0.1). Plasma IGF-I increased continuously during the insulin infusion, and reached the highest concentrations at the end of the clamp, being almost 4-fold higher in INS compared with CTL cows (117+/-4 vs 30+/-4 ng/ml; P<0.001). Hepatic expression of GHR 1A and IGF-I mRNA was low in CTL cows, but was increased 3.6-fold (P<0.05) and 6.3-fold (P<0.001) respectively in INS cows. By contrast, in adipose tissue the changes in gene expression in response to insulin were reversed with decreases in both total GHR and IGF-I mRNA. The expressions of GHR 1A and IGF-I mRNA in liver tissue were correlated in INS (r=0.86; P<0.05), but not CTL cows (r=0.43; P>0.1). Insulin appears to be a key metabolic signal in coupling the GH-IGF axis, thus orchestrating a marked elevation in circulating IGF-I concentrations.

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Effect of negative energy balance on the insulin-like growth factor system in pre-recruitment ovarian follicles of post partum dairy cows

Reproduction ◽

10.1530/rep-06-0122 ◽

2007 ◽

Vol 133 (3) ◽

pp. 627-639 ◽

Cited By ~ 26

Author(s):

S Llewellyn ◽

R Fitzpatrick ◽

D A Kenny ◽

J J Murphy ◽

R J Scaramuzzi ◽

...

Keyword(s):

Energy Balance ◽

Mrna Expression ◽

Post Partum ◽

Ovarian Follicles ◽

Negative Energy ◽

Negative Energy Balance ◽

Igf Binding Proteins ◽

Igf I ◽

Igf Binding ◽

Ovarian Cyclicity

Post partumnegative energy balance (NEB) in dairy cattle is associated with a delayed return to ovarian cyclicity and reduced fertility. This study compared the IGF system of pre-recruitment ovarian follicles between cows in mild (n= 6) or severe (n= 6) NEB during early lactation. Ovaries were collected in the second weekpost partum, when circulating concentrations of IGF-I and glucose were lower (P< 0.01) in severe NEB cows. mRNA expression for IGF-II, type 1 IGF receptor (IGF-1R) and IGF-binding proteins (IGFBP)-1 to IGFBP-6 was determined byin situhybridisation in individual follicles using radiolabelled oligonucleotide probes. Follicles were classified as very small (1–2.5 mm) or small (2.5–5 mm) and healthy or atretic. Relative mRNA concentrations were measured as optical density (OD) units using image analysis. Thecal IGF-II mRNA expression was highest in very small, healthy follicles (P< 0.05). Granulosa cell IGFBP-2 was the only component to change with EB status, with higher mRNA expression in mild compared with severe NEB cows (P< 0.05). IGFBP-1 and IGFBP-3 mRNA expression were undetectable. IGF-1R, IGFBP-4 and IGFBP-5 mRNA expression were not significantly altered by follicle size or health, but IGFBP-5 tended to increase in atretic follicles. The pattern of IGFBP-6 mRNA expression in theca paralleled that of IGF-II mRNA, with higher (P< 0.05) levels in healthy, very small follicles. In conclusion, the reduced expression of IGFBP-2 mRNA in severe NEB cows may alter the bioavailability of circulating IGF-I and locally produced IGF-II to modulate the pre-recruitment stages of follicles required to maintain normalpost partumovarian cyclicity.

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Effect of season and photoperiod on the time of first postpartum ovulation in Awassi ewes

Acta Veterinaria Hungarica ◽

10.1556/avet.2011.036 ◽

2011 ◽

Vol 59 (4) ◽

pp. 497-510 ◽

Cited By ~ 2

Author(s):

Vera Faigl ◽

Mónika Keresztes ◽

Alíz Márton ◽

Hedvig Fébel ◽

Margit Kulcsár ◽

...

Keyword(s):

Energy Balance ◽

Ovarian Function ◽

Post Partum ◽

Negative Energy ◽

Negative Energy Balance ◽

Plasma Metabolites ◽

Control Group ◽

Ovarian Activity ◽

Seasonal Differences ◽

Igf I

Seasonal differences in the resumption of postpartum ovarian activity, milk production and periparturient metabolic status were investigated in lactating non-suckling dairy Awassi sheep in two consecutive experiments. In Experiment 1, autumn-lambing (AL, n = 27) and spring-lambing (SL, n = 37) ewes were investigated. Ovarian activity was monitored by means of individual progesterone (P4) profiles from day 5 to day 100 post partum. Most of the AL dams (89%) ovulated till day 35 after parturition and became cyclic thereafter. Incidence of persistent corpus luteum (CLP) and short luteal phases (sCL) was frequent (18% and 29%, respectively) among non-conceiving dams. In contrast, only 24% of the SL ewes ovulated before day 35. P4 levels during the luteal phase were lower in cyclic animals, and the cycle was longer in SL than in AL animals. No CLP or sCL was detected in the spring-lambing group, and 61% of SL ewes remained acyclic till the end of the trial. Lactation length was significantly longer in SL dams than in AL ewes (P = 0.008). According to the plasma metabolites (BHB, NEFA) and metabolic hormones (insulin, IGF-I, thyroxine) examined, negative energy balance did not appear in any of the animals. However, seasonal differences were seen in IGF-I and thyroxine levels, which were higher in the SL dams. In Experiment 2, influence of additional lighting was studied in autumn-lambing ewes. The long-day photoperiod (LD, n = 23) group was exposed to artificial light from sunset till midnight (approx. 16 h light/8 h dark) from some weeks before the expected date of delivery in mid-September until the end of December. The control group (n = 25) experienced only natural daylength. The first postpartum ovulation tended to occur later in the LD animals than in the controls (P = 0.047). The lactation of the LD group tended to be longer (P = 0.061). NEFA, BHB, insulin, IGF-I and thyroxine levels did not differ between the groups. Conclusions: (i) The ovarian function of the Awassi population is seasonal under temperate continental climate conditions. (ii) The first postpartum ovulation of non-suckling, autumn-lambing dams may occur very early, even before the completion of uterine involution. (iii) Additional artificial lighting may delay the time of first postpartum ovulation in AL ewes. (iv) Postpartum negative energy balance is unlikely to occur in dairy Awassi ewes even in high-producing intensive systems.

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Differing adaptations of IGF-I and its IGFBPs in dairy cows during a negative energy balance in early lactation and a negative energy balance induced by feed restriction in mid-lactation

Veterinární Medicína ◽

10.17221/7030-vetmed ◽

2013 ◽

Vol 58 (No. 9) ◽

pp. 459-467 ◽

Cited By ~ 5

Author(s):

EC Kessler ◽

JJ Gross ◽

RM Bruckmaier

Keyword(s):

Energy Balance ◽

Dairy Cows ◽

Negative Energy ◽

Mrna Abundance ◽

Negative Energy Balance ◽

Feed Restriction ◽

Time Points ◽

Period 2 ◽

Igf I ◽

Igfbp 3

: Control of metabolic pathways is a major task of the somatotropic axis and its constituents. Insulin-like growth-factor binding proteins (IGFBPs) bind IGF-I and -II and act as carriers and regulators of their activities in blood, body fluids and tissues. Over two periods of physiological adaptation, this study investigated the binding pattern of IGF-I to IGFBPs in the plasma of 50 multiparous Holstein dairy cows and identified relationships with the hepatic mRNA abundance of IGFBPs and plasma IGF-I during the lactational negative energy balance (NEB) and during a deliberately induced NEB by feed restriction. Period 1 lasted from week 3 antepartum (a.p.) to week 12 postpartum (p.p.) and period 2, the period of feed restriction, started at around 100 DIM and lasted for three weeks with a control (C) and a restricted group (R). Blood samples and liver biopsies were collected in week 3 a.p., and in weeks 1 and 4 p.p. of period 1 and in weeks 0 and 3 of period 2. For column chromatography of IGFBPs, plasma samples of all animals were pooled by group and time points of sampling. Plasma IGF-I dropped from week 3 a.p. to week 1 p.p. and thereafter increased until week 0 (period 2) and did not change up to week 3 of period 2. The binding of IGF-I to plasma IGFBP-1 and -2 increased in period 1 from week 3 a.p. to week 4 p.p., while at the same time it decreased for IGFBP-3. During period 2, the binding of IGF-I to plasma IGFBP-1 and -2 decreased for both groups, but less for R cows. In C cows, the IGF-I binding to IGFBP-3 in plasma increased from week 0 to week 3 of period 2, whereas R cows showed a slight decrease. In period 1, hepatic mRNA abundance of IGFBP-3 followed the plasma IGFBP-3 binding in contrast to the mRNA abundances of IGFBP-1 and -2. The latter increased from week 3 a.p. to week 1 p.p. and decreased afterwards whereas IGF-I binding to IGFBP-1 and -2 increased. In week 3 of period 2, the binding of IGF-I to IGFBP-1 and -2 and their hepatic mRNA abundance were higher in R cows compared to C cows. Hepatic mRNA abundance of IGF-I was consistently positively correlated with plasma IGF-I, especially pronounced during the NEBs in week 1 p.p. (period 1) and in week 3 (period 2) in R cows. While no distinct relation between mRNA abundance of IGFBP-1 and plasma IGF-I was evident, the mRNA abundance of IGFBP-2 was inversely related to plasma IGF-I over all experimental time points independent of treatment. The mRNA abundance of IGFBP-3 was particularly correlated with plasma IGF-I during the 2 experimental stages of a NEB. Obviously IGFBP-3, but not IGFBP-1 and -2, binding in plasma closely followed the respective pattern of hepatic mRNA abundance during the entire experimental period. The fact that changes in the different plasma IGFBPs during altering metabolic stages in different stages of lactation do not always strictly follow their mRNA abundance in liver suggests tissues other than the liver flexibly contributing to the IGFBP pool in plasma as well as a partially post-transcriptional regulation of IGFBP synthesis.  

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Follicular dynamics, plasma metabolites, hormones and insulin-like growth factor I (IGF-I) in lactating cows with positive or negative energy balance during the preovulatory period

Reproduction Nutrition Development ◽

10.1051/rnd:19920403 ◽

1992 ◽

Vol 32 (4) ◽

pp. 331-341 ◽

Cited By ~ 46

Author(s):

MC Lucy ◽

J. Beck ◽

CR Staples ◽

HH Head ◽

RL De La Sota ◽

...

Keyword(s):

Growth Factor ◽

Energy Balance ◽

Negative Energy ◽

Negative Energy Balance ◽

Plasma Metabolites ◽

Insulin Like Growth Factor ◽

Factor I ◽

Lactating Cows ◽

Igf I ◽

Follicular Dynamics

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Leptin Modulates the Response of Brown Adipose Tissue to Negative Energy Balance: Implication of the GH/IGF-I Axis

International Journal of Molecular Sciences ◽

10.3390/ijms22062827 ◽

2021 ◽

Vol 22 (6) ◽

pp. 2827

Author(s):

Vicente Barrios ◽

Laura M. Frago ◽

Sandra Canelles ◽

Santiago Guerra-Cantera ◽

Eduardo Arilla-Ferreiro ◽

...

Keyword(s):

Adipose Tissue ◽

Energy Balance ◽

Brown Adipose Tissue ◽

Uncoupling Protein ◽

Negative Energy ◽

Negative Energy Balance ◽

Cytokine Signaling ◽

Mrna Levels ◽

Brown Adipose ◽

Igf I

The growth hormone (GH)/insulin-like growth factor I (IGF-I) axis is involved in metabolic control. Malnutrition reduces IGF-I and modifies the thermogenic capacity of brown adipose tissue (BAT). Leptin has effects on the GH/IGF-I axis and the function of BAT, but its interaction with IGF-I and the mechanisms involved in the regulation of thermogenesis remains unknown. We studied the GH/IGF-I axis and activation of IGF-I-related signaling and metabolism related to BAT thermogenesis in chronic central leptin infused (L), pair-fed (PF), and control rats. Hypothalamic somatostatin mRNA levels were increased in PF and decreased in L, while pituitary GH mRNA was reduced in PF. Serum GH and IGF-I concentrations were decreased only in PF. In BAT, the association between suppressor of cytokine signaling 3 and the IGF-I receptor was reduced, and phosphorylation of the IGF-I receptor increased in the L group. Phosphorylation of Akt and cyclic AMP response element binding protein and glucose transporter 4 mRNA levels were increased in L and mRNA levels of uncoupling protein-1 (UCP-1) and enzymes involved in lipid anabolism reduced in PF. These results suggest that modifications in UCP-1 in BAT and changes in the GH/IGF-I axis induced by negative energy balance are dependent upon leptin levels.

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