High-Resolution Time-of-Flight Spectra Obtained Using the MULTUM II Multi-Turn Type Time-of-Flight Mass Spectrometer with an Electron Ionization Ion Source

2005 ◽  
Vol 11 (3) ◽  
pp. 261-266 ◽  
Author(s):  
Daisuke Okumura ◽  
Michisato Toyoda ◽  
Morio Ishihara ◽  
Itsuo Katakuse
Keyword(s):  
Mass Spectrometer ◽  
Mass Difference ◽  
Time Of Flight ◽  
Ion Source ◽  
Electron Ionization ◽  
Mass Resolution ◽  
Resolving Power ◽  
High Mass ◽  
Electric Sector ◽  
Mass Resolving Power

This paper describes experiments demonstrating the high mass-resolving power of the MULTUM II multi-turn type time-of-flight (ToF) mass spectrometer with a 1.308-meter circuit controlled by four toroidal electric sector fields1 and an electron ionization (EI) ion source. A mass resolution of 250,000 [full-width at half maximum: (FWHM)] was obtained for N2+ after a flight time of 9.0 ms (flight cycles: 1200, flight length: 1500 m). A doublet of 12C5H514N and 13C12C5H6 ( m/Δ m = 9746; Δ m: mass difference of doublet, m: mass of lighter ion of doublet) was separated and a mass resolution of 91,000 (FWHM) was obtained. A doublet of CDCl2 and CH2Cl2 ( m/Δ m = 54,162) was also separated. A mass resolution of 115,000 (FWHM) was then achieved. When one peak of these doublets was used as a calibrant, the mass of the other peak was determined within a few ppm by mass difference. The ToF depending on the square of m/z was significantly larger than the systematic errors in the ToF, so that good mass accuracy was obtained by one-point mass determination.

scholarly journals Limits for resolving tandem mass tag reporter ions with identical integer mass using phase constrained spectrum deconvolution

2018 ◽  
Author(s):  
Christian D. Kelstrup ◽  
Konstantin Aizikov ◽  
Tanveer S. Batth ◽  
Arne Kreutzman ◽  
Dmitry Grinfeld ◽  
...  
Keyword(s):  
Mass Spectrometer ◽  
Peptide Sequencing ◽  
Mass Resolution ◽  
Resolving Power ◽  
Acquisition Time ◽  
Tandem Mass ◽  
Optimal Method ◽  
Orbitrap Mass Spectrometer ◽  
Mass Resolving Power ◽  
Spectrum Deconvolution

ABSTRACTA popular method for peptide quantification relies on isobaric labeling such as tandem mass tags (TMT) which enables multiplexed proteome analyses. Quantification is achieved by reporter ions generated by fragmentation in a tandem mass spectrometer. However, with higher degrees of multiplexing, the smaller mass differences between the reporter ions increase the mass resolving power requirements. This contrasts with faster peptide sequencing capabilities enabled by lowered mass resolution on Orbitrap instruments. It is therefore important to determine the mass resolution limits for highly multiplexed quantification when maximizing proteome depth. Here we defined the lower boundaries for resolving TMT reporter ions with 0.0063 Da mass differences using an ultra-high-field Orbitrap mass spectrometer. We found the optimal method depends on the relative ratio between closely spaced reporter ions and that 64 ms transient acquisition time provided sufficient resolving power for separating TMT reporter ions with absolute ratio changes up to 16-fold. Furthermore, a 32 ms transient processed with phase-constrained spectrum deconvolution provides >50% more identifications with >99% quantified, but with a slight loss in quantification precision and accuracy. These findings should guide decisions on what Orbitrap resolution settings to use in future proteomics experiments relying on TMT reporter ion quantification with identical integer masses.

High mass resolution glow discharge mass spectrometry using an external ion source FT-ICR mass spectrometer

1993 ◽  
Vol 65 (20) ◽  
pp. 2801-2804 ◽  
Author(s):  
Clifford H. Watson ◽  
John. Wronka ◽  
Frank H. Laukien ◽  
Christopher M. Barshick ◽  
John R. Eyler
Keyword(s):  
Mass Spectrometry ◽  
Glow Discharge ◽  
Mass Spectrometer ◽  
Ion Source ◽  
Mass Resolution ◽  
Glow Discharge Mass Spectrometry ◽  
High Mass ◽  
High Mass Resolution ◽  
Ft Icr

Time-of-flight mass spectrographs of high mass resolving power

2019 ◽  
Vol 34 (36) ◽  
pp. 1942001
Author(s):  
H. Wollnik ◽  
M. Wada ◽  
P. Schury ◽  
M. Rosenbusch ◽  
Y. Ito ◽  
...  
Keyword(s):  
Time Of Flight ◽  
Molecular Ions ◽  
Resolving Power ◽  
High Mass ◽  
Energetic Ions ◽  
Structure Of Molecules ◽  
Mass Resolving Power ◽  
Sum Formula ◽  
The Masses

The masses of charged atoms and molecules were first investigated by laterally dispersive sector field mass analyzers, which early on already achieved high mass resolving powers. Equally, high mass resolving powers were achieved by time-of-flight mass analyzers during the last decades. These measurements became possible when fast and precise electronic circuitries became available. Such techniques have been developed and used extensively for the mass analysis of short-lived nuclei, whose mass values reveal insight in processes that describe the formation of elements in star explosions. Precise mass determinations of short-lived ions have been performed for energetic ions in large accelerator storage rings as well as for low-energy ions in time-of-flight mass spectrographs with long flight paths. Similarly, precise mass measurements can also be performed for molecular ions that help to reveal the structure of molecules. In case of very high mass resolving powers, the mass determination of molecular ions can be so high that the measured ion mass directly reveals the molecule’s sum formula.

Simultaneous Hydrogen and Heavier Element Isotopic Ratio Images with a Scanning Submicron Ion Probe and Mass Resolved Polyatomic Ions

2014 ◽  
Vol 20 (2) ◽  
pp. 577-581 ◽  
Author(s):  
Georges Slodzian ◽  
Ting-Di Wu ◽  
Noémie Bardin ◽  
Jean Duprat ◽  
Cécile Engrand ◽  
...  
Keyword(s):  
Secondary Ion Mass Spectrometry ◽  
Isotopic Ratio ◽  
Simultaneous Recording ◽  
Mass Resolution ◽  
Resolving Power ◽  
High Mass ◽  
Ratio Measurement ◽  
Ion Probe ◽  
Secondary Ions ◽  
Mass Resolving Power

AbstractIn situ microanalysis of solid samples is often performed using secondary ion mass spectrometry (SIMS) with a submicron ion probe. The destructive nature of the method makes it mandatory to prevent information loss by using instruments combining efficient collection of secondary ions and a mass spectrometer with parallel detection capabilities. The NanoSIMS meets those requirements with a magnetic spectrometer but its mass selectivity has to be improved for accessing opportunities expected from polyatomic secondary ions. We show here that it is possible to perform D/H ratio measurement images using 12CD−/12CH−, 16OD−/16OH−, or 12C2D−/12C2H− ratios. These polyatomic species allow simultaneous recording of D/H ratios and isotopic compositions of heavier elements like 15N/14N (via 12C15N−/12C14N−) and they provide a powerful tool to select the phase of interest (e.g., mineral versus organics). We present high mass resolution spectra and an example of isotopic imaging where D/H ratios were obtained via the 12C2D−/12C2H− ratio with 12C2D− free from neighboring mass interferences. Using an advanced mass resolution protocol, a “conventional” mass resolving power of 25,000 can be achieved. Those results open many perspectives for isotopic imaging at a fine scale in biology, material science, geochemistry, and cosmochemistry.

scholarly journals An ion trap time-of-flight mass spectrometer with high mass resolution for cold trapped ion experiments

2017 ◽  
Vol 88 (12) ◽  
pp. 123107 ◽  
Author(s):  
P. C. Schmid ◽  
J. Greenberg ◽  
M. I. Miller ◽  
K. Loeffler ◽  
H. J. Lewandowski
Keyword(s):  
Mass Spectrometer ◽  
Ion Trap ◽  
Time Of Flight ◽  
Mass Resolution ◽  
High Mass ◽  
Trapped Ion ◽  
Flight Mass Spectrometer ◽  
High Mass Resolution

Development of an Ion Trap/Multi-Turn Time-of-Flight Mass Spectrometer with Potential-Lift

2009 ◽  
Vol 15 (2) ◽  
pp. 249-260 ◽  
Author(s):  
Kenichi Iwamoto ◽  
Hirofumi Nagao ◽  
Michisato Toyoda
Keyword(s):  
Mass Spectrometer ◽  
Cross Sections ◽  
Ion Trap ◽  
Time Of Flight ◽  
Paul Trap ◽  
Ion Source ◽  
Mass Resolution ◽  
Storage Device ◽  
Linear Mode ◽  
New Apparatus

An ion trap/multi-turn time-of-flight (ToF) mass spectrometer with potential-lift has been developed. This system consists of an external ion source, a lens system, an ion trap, a potential-lift, a multi-turn ToF mass spectrometer and a detector. The ion trap consists of hyperbolic electrode cross-sections (Paul trap) and is used as an ion storage device. The potential-lift, which is part of the flight tube, was attached between the ion trap and the multi-turn ToF mass spectrometer. The potential-lift is known to be useful for increasing the kinetic energy of the ions. In order to check the ability of the potential-lift, mass distributions of [(CsI) n Cs]+ clusters ( n = 1–9) were measured. The relative intensity ratios of the [(CsI) nCs]+ clusters were consistent with the results obtained using other apparatus. To check the properties of the new apparatus, Xe+ isotopes were analyzed using either a linear or multi-turn ToF mass spectrometer. In the linear mode, the mass resolution was 500. In the multi-turn mode, the resolution depended on the number of cycles of the multi-turn ToF mass spectrometer; the mass resolution was 4400 (FWHM) after nine cycles. This new apparatus with a high resolution will be useful for measurements of ion–molecule reactions and photodissociations.

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