Frontline ® immunochromatographic device for on-site urine testing of amphetamines: laboratory validation using authentic specimens

2000 ◽  
Vol 37 (2) ◽  
pp. 199-204 ◽  
Author(s):  
O Beck ◽  
M Kraft ◽  
M R Moeller ◽  
B L Smith ◽  
S Schneider ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Close Agreement ◽  
Flame Ionization Detector ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Detector ◽  
Test Device ◽  
Flame Ionization ◽  
Colour Scale ◽  
Urine Testing

We evaluated a new test device for amphetamines and methamphetamines (Frontline ®, cut-off limit 300 ng/mL) using authentic clinical and forensic specimens. The device is based on immunochromatography and is dipped into urine and read visually by comparison with a colour scale after a few minutes. A total of 658 specimens were tested by comparing results of the screening procedure with established immunoassays. Discordant results were further investigated by gas chromatography- mass spectrometry or gas chromatography (with flame ionization detector). The Frontline device had a sensitivity of 93% and a specificity of 98%. When specimens were classified by urine amphetamine concentration, close agreement was obtained at concentrations below 150 ng/mL and above 1000 ng/mL. A small number of specimens with amphetamine concentrations between 300 and 1000 ng/mL tested negative in the Frontline test. This finding could to some extent be explained by the enantioselectivity of the antibodies in the Frontline test to d-amphetamine. We conclude that the performance of the Frontline test device for amphetamines is adequate for presumptive clinical and forensic screening.

Characterization of Agarwood Incense using Gas Chromatography - Mass Spectrometry (GC-MS) coupled with Solid Phase Micro Extraction (SPME) and Gas Chromatography - Flame Ionization Detector (GC-FID)

2015 ◽  
Vol 12 (2) ◽  
pp. 67
Author(s):  
Nurlaila Ismail ◽  
Mastura Lbrahim ◽  
Seema Zareen ◽  
Mohd Hezri Fazalul Rahiman ◽  
Saiful Nizam Tajuddin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Solid Phase ◽  
Flame Ionization Detector ◽  
Gas Chromatography Mass Spectrometry ◽  
Solid Phase Micro Extraction ◽  
Ionization Detector ◽  
Polydimethyl Siloxane ◽  
Flame Ionization ◽  
Chromatography Mass Spectrometry

This paper presents the application of Solid Phase Micro Extraction (SPME) coupled with Gas Chromatography-Mass Spectrometry (GC-MS) and Gas Chromatography-Flame Ionization Detector (GC-FID) to charaterize agarwood incense. The work involved three types of SPME fibres at 30 minutes sampling time. The fibres are 50/30 µm divinylbenzene-carboxen­polydimethysiloxane (DVB-CAR-PDMS), 65 µm polydi methylsiloxane­divinylbenzene (PDMS-DVB) and 85 µm carboxen-polydimethyl siloxane (CAR-PDMS). The results showed that among many compounds extracted by GC-MS coupled with SPME, six compounds were found significantly presence in high quality aganvood incense due to their high percentage area (%). They are beta-maaliene, alpha-elemol, beta-selinene, 10-epi-gamma-eudesmol, agarospirol and caryophellene oxide. The finding offers a new approach for establishing the volatile profile of agarwood incense components which is beneficial for agarwood grading and discrimination.

Characterization of Agarwood Incense using Gas Chromatography - Mass Spectrometry (GC-MS) coupled with Solid Phase Micro Extraction (SPME) and Gas Chromatography - Flame Ionization Detector (GC-FID)

2015 ◽  
Vol 12 (2) ◽  
pp. 67
Author(s):  
Nurlaila Ismail ◽  
Mastura Lbrahim ◽  
Seema Zareen ◽  
Mohd Hezri Fazalul Rahiman ◽  
Saiful Nizam Tajuddin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Solid Phase ◽  
Flame Ionization Detector ◽  
Gas Chromatography Mass Spectrometry ◽  
Solid Phase Micro Extraction ◽  
Ionization Detector ◽  
Polydimethyl Siloxane ◽  
Flame Ionization ◽  
Chromatography Mass Spectrometry

This paper presents the application of Solid Phase Micro Extraction (SPME) coupled with Gas Chromatography-Mass Spectrometry (GC-MS) and Gas Chromatography-Flame Ionization Detector (GC-FID) to charaterize agarwood incense. The work involved three types of SPME fibres at 30 minutes sampling time. The fibres are 50/30 µm divinylbenzene-carboxen­polydimethysiloxane (DVB-CAR-PDMS), 65 µm polydimethylsiloxane­divinylbenzene (PDMS-DVB) and 85 µm carboxen-polydimethyl siloxane (CAR-PD MS). The results showed that among many compounds extracted by GC-MS coupled with SPME, six compounds were found significantly presence in high quality aganvood incense due to their high percentage area(%). They are b-maaliene, a-elemol, b-selinene, 10-epi-y-eudesmol, agarospirol and caryophellene oxide. The finding offers a new approach for establishing the volatile profile of agarwood incense components which is beneficial for agarwood grading and discrimination. 

scholarly journals Chemical Composition of Vernonia albicans Essential Oil from India

2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900
Author(s):  
Rajesh K. Joshi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Flame Ionization Detector ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Aerial Parts ◽  
Sesquiterpene Hydrocarbons ◽  
Total Oil

The chemical composition of the hydro-distilled essential oil obtained from the flowering aerial parts of Vernonia albicans DC. (Asteraceae) was analyzed by gas chromatography equipped with a flame ionization detector (GC-FID) and gas chromatography coupled with a mass spectrometry (GC/MS). Thirty-nine compounds have been identified, representing 97.5% of the total oil. The major constituents were β-caryophyllene (34.3%), γ-amorphene (19.5%), 9-epi-β-caryophyllene (6.9%), and α-pinene (6.9%). The oil was found to be rich in sesquiterpene hydrocarbons (73.9%).

Chemical Composition of the Essential Oil From Minthostachys acris Schmidt-Leb. Grown in the Peruvian Andes

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1985749
Author(s):  
Jorge A. Pino ◽  
María Milagros Dueñas-Mendoza ◽  
Leoncio Solís-Quispe
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Steam Distillation ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Detector ◽  
Peruvian Andes ◽  
Flame Ionization ◽  
Aerial Parts

The chemical composition of the essential oil from aerial parts of Minthostachys acris Schmidt-Leb. grown in Cuzco was studied. A total of 59 volatile compounds were identified by gas-chromatography-flame ionization detector and gas chromatography-mass spectrometry in the essential oil obtained by steam distillation, of which the most prominent were pulegone (54.4%), cis-menthone (11.0%), and thymol (6.3%).

Sign in / Sign up

Export Citation Format

Share Document