scholarly journals Nephrocalcinosis in 2 young rats: a case report

1980 ◽  
Vol 14 (3) ◽  
pp. 241-242 ◽  
Author(s):  
Jerald Silverman ◽  
Abraham Rivenson
Keyword(s):  
Case Report ◽  
Histopathological Finding ◽  
Sprague Dawley ◽  
Young Rats ◽  
Corticomedullary Junction ◽  
Sprague Dawley Rats

Nephrocalcinosis was diagnosed as an incidental histopathological finding in 2 Sprague-Dawley rats of 150 g bodyweight, The calcified material was located along the entire corticomedullary junction. Dietary and other causes of nephrocalcinosis are discussed.

Testosterone, dihydrotestosterone and androstanediols in plasma, testes and prostates of rats during development

1981 ◽  
Vol 96 (1) ◽  
pp. 127-135 ◽  
Author(s):  
Colette Corpéchot ◽  
Etienne-Emile Baulieu ◽  
Paul Robel
Keyword(s):  
Plasma Concentration ◽  
Sharp Peak ◽  
Ventral Prostate ◽  
Sprague Dawley ◽  
Adult Level ◽  
Broad Maximum ◽  
Young Rats ◽  
Sprague Dawley Rats ◽  
Radio Immunoassay ◽  
Specific Radio

Abstract. Testosterone (T). 5α-dihydrotestosterone (DHT), 5α-androstane-3α, 17β-diol (3α-diol), and 5α-androstane-3β,17β-diol (3β-diol) have been measured in the plasma, ventral prostate, and testes of Sprague-Dawley rats with a specific radio-immunoassay, at birth, and then weekly until the 12th week. In plasma, the T concentration is ∼ 0.3 ng/ml at birth and up to the 4th week; thereafter it increases steadily and reaches the adult level of ∼ 3 ng/ml by the 8th week. In male foetuses of 18 days gestation, the plasma concentration is 0.6 ng/ml. The 5α-metabolites taken together are at a higher concentration than that of T until the 7th week, and each one, after a broad maximum between 5–9 weeks, decreases promptly by the 10th week. The larger formation of 5α-metabolites from T in young rats has been confirmed by injection of the hormone to castrated males, which showed relatively more conversion at 5 than at 10 weeks. In the ventral prostate, DHT is already high at 4 weeks (11 ng/g), and remain between 7 and 15 ng/g until the 12th week. T concentration is also larger than in plasma at 4th week (2 ng/g) and becomes approximately equal to the plasma value at puberty. A sharp peak of 3α-diol and 3β-diol is observed at the 8th week. In the testes, T concentration is high at birth (183 ng/g), then declines rapidly, and increases again from the 5th week onward in parallel with plasma concentration. The concentrations of 5α-metabolites per testis vary in parallel to those in plasma, with the exception of sustained elevation of 3β-diol.

scholarly journals Case Report on Situs Inversus Totalis in Two Sprague-Dawley Rats.

1995 ◽  
Vol 44 (4) ◽  
pp. 341-345 ◽  
Author(s):  
Kazumi SHIRAIWA ◽  
Atsushi WATANABE ◽  
Norihiro SATO ◽  
Midori NITO ◽  
Youshiro KOBAYASHI
Keyword(s):  
Case Report ◽  
Situs Inversus ◽  
Situs Inversus Totalis ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

Abstract P202: Impaired Central, Renal, and Blood Pressure Responses to Alterations in Fluid and Electrolyte Homeostasis in Aged Sprague-Dawley Rats

Hypertension ◽  
2016 ◽  
Vol 68 (suppl_1) ◽  
Author(s):  
Alissa A Frame ◽  
Kyle R Rodrigues ◽  
Lillian M Whelan ◽  
Richard D Wainford
Keyword(s):  
Human Subjects ◽  
Sodium Reabsorption ◽  
Electrolyte Balance ◽  
Aged Rats ◽  
Neuronal Activation ◽  
Sprague Dawley ◽  
Young Rats ◽  
Sprague Dawley Rats ◽  
Electrolyte Homeostasis ◽  
Parvocellular Neurons

Aim: Hypertension is strongly correlated with increased age in human subjects. These studies tested the hypothesis that impaired natriuretic responses to acute and chronic challenges to fluid and electrolyte homeostasis contribute to age-dependent hypertension. Methods: Two-month old (young) and 6-month old (aged) male Sprague-Dawley rats underwent an acute IV volume expansion (VE; 5% BW) and MAP, HR, urine output, and PVN neuronal activation (c-Fos expression) were assessed (n=4 per group). In a separate study, naïve 2 and 6-month old male Sprague-Dawley rats were maintained on a normal (0.6% NaCl) or high salt (4% NaCl) diet, and on day 21, MAP and NCC activity (peak natriuresis to IV HCTZ, 2mg/kg) were assessed (n=4 per group). Results: Renal excretion of sodium and water after acute VE was impaired in aged rats (total % sodium load excreted; young 78±6 vs aged 60±7: total % water load excreted; young 96±7 vs aged 66±5, P<0.05). PVN neuronal activation was attenuated in response to acute VE in aged rats in all parvocellular regions excluding the lateral parvocellular subnucleus (PVN neuronal activation [c-fos positive cells]; medial parvocellular young 59±4 vs aged 42±7, P<0.05). CV parameters did not change in response to acute VE in either group. Chronic HS diet evoked an increase in MAP in aged rats but not young rats (MAP [mmHg]; young NS 124±2 vs young HS 126±3 vs aged HS 138±3, P<0.05). Chronic HS diet caused a decrease in NCC activity in young rats and, in contrast, an increase in aged rats (peak ΔUNaV to HCTZ [μeq/min]; young NS 9.2±0.5 vs young HS 7.1±0.3 vs aged HS 16±1, P<0.05). Conclusion: Activation of sympathoinhibitory PVN parvocellular neurons is a well-characterized response to acute VE. Our data suggest there are attenuated acute PVN sympathoinhibitory responses to alterations in fluid and electrolyte balance in aged rats. In aged animals, HS intake increased NCC-mediated sodium reabsorption and promoted the development of sodium-dependent hypertension. We speculate that this is driven by blunted HS-evoked sympathoinhibition, likely due to reduced PVN neuronal activation.

Ultrastructural Investigation of Spontaneous Pituitary Adenomas in Aging Sprague-Dawley Rats

1982 ◽  
Vol 40 ◽  
pp. 216-217
Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs
Keyword(s):  
Microscopic Study ◽  
Pituitary Adenomas ◽  
Wistar Rats ◽  
Microscopic Level ◽  
Sprague Dawley ◽  
Prolactin Cells ◽  
Light Microscopic Level ◽  
Ultrastructural Investigation ◽  
Sprague Dawley Rats ◽  
Long Evans

Investigation of the spontaneous pituitary adenomas in rat have been limited mainly to light microscopic study. Furth et al. (1973) described them as chromophobic, secreting prolactin. Kovacs et al. (1977) in an ul trastructural investigation of adenomas of old female Long-Evans rats, found that they were composed of prolactin cells. Berkvens et al. (1980) using immunocytochemistry at the light microscopic level, demonstrated that some spontaneous tumors of old Wistar rats could contain GH, TSH or ACTH as well as PRL.

Early Development of Drug-Induced Hepatic Necrosis

1971 ◽  
Vol 29 ◽  
pp. 576-577
Author(s):  
F. G. Zaki ◽  
E. Detzi ◽  
C. H. Keysser
Keyword(s):  
Early Development ◽  
Hepatic Necrosis ◽  
Screening Tests ◽  
Dense Matrix ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Drug Induced ◽  
Hepatocellular Damage ◽  
Sprague Dawley Rats ◽  
Microscopic Studies

This study represents the first in a series of investigations carried out to elucidate the mechanism(s) of early hepatocellular damage induced by drugs and other related compounds. During screening tests of CNS-active compounds in rats, it has been found that daily oral administration of one of these compounds at a dose level of 40 mg. per kg. of body weight induced diffuse massive hepatic necrosis within 7 weeks in Charles River Sprague Dawley rats of both sexes. Partial hepatectomy enhanced the development of this peculiar type of necrosis (3 weeks instead of 7) while treatment with phenobarbital prior to the administration of the drug delayed the appearance of necrosis but did not reduce its severity.Electron microscopic studies revealed that early development of this liver injury (2 days after the administration of the drug) appeared in the form of small dark osmiophilic vesicles located around the bile canaliculi of all hepatocytes (Fig. 1). These structures differed from the regular microbodies or the pericanalicular multivesicular bodies. They first appeared regularly rounded with electron dense matrix bound with a single membrane. After one week on the drug, these vesicles appeared vacuolated and resembled autophagosomes which soon developed whorls of concentric lamellae or cisterns characteristic of lysosomes (Fig. 2). These lysosomes were found, later on, scattered all over the hepatocytes.

Ultrastructural investigation of spontaneous pituitary gonadotroph cell adenomas in aging Sprague-Dawley rats

1983 ◽  
Vol 41 ◽  
pp. 702-703
Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs
Keyword(s):  
Electron Microscopy ◽  
Animal Model ◽  
Epoxy Resin ◽  
Immunoelectron Microscopy ◽  
Tumor Type ◽  
Gonadal Function ◽  
Sprague Dawley ◽  
Male And Female ◽  
Reticulin Fibers ◽  
Sprague Dawley Rats

Gonadotroph cell adenomas of the pituitary are infrequent in human patients and are not invariably associated with altered gonadal function. To date, no animal model of this tumor type exists. Herein, we describe spontaneous gonadotroph cell adenomas in old male and female Sprague-Dawley rats by histology, immunocytology and electron microscopy.The material consisted of the pituitaries of 27 male and 38 female Sprague Dawley rats, all 26 months of age or older, removed at routine autopsy. Sections of formal in-fixed, paraffin-embedded tissue were stained with hematoxylin-phloxine-saffron (HPS), the PAS method and the Gordon-Sweet technique for the demonstration of reticulin fibers. For immunostaining, sections were exposed to anti-rat β-LH, anti-ratβ-TSH, anti-rat PRL, anti-rat GH and anti-rat ACTH 1-39. For electron microscopy, tissue was fixed in 2.5% glutaraldehyde, postfixed in 1% OsO4 and embedded in epoxy-resin. Tissue fixed in 10% formalin, embedded in epoxy resin without osmification, was used for immunoelectron microscopy.

Freeze-etch studies of epiphyseal growth plate cartilage reveal matrix vesicles associated with calcification

1978 ◽  
Vol 36 (2) ◽  
pp. 670-671
Author(s):  
Russell N. A. Cecil ◽  
H. Clarke Anderson
Keyword(s):  
Chromic Acid ◽  
Light And Electron Microscopy ◽  
Matrix Vesicles ◽  
Matrix Vesicle ◽  
Glycerol Solution ◽  
Sprague Dawley ◽  
Epiphyseal Growth Plate ◽  
Growth Plates ◽  
Sprague Dawley Rats ◽  
Tibial Epiphyseal

Unfixed proximal tibial epiphyseal growth plates were studied by freeze-etch to confirm the presence of extracellular calcifying matrix vesicles and to determine the substructure of matrix vesicle membranes as compared to plasma and other membranes of intact chondrocytes. Growth plates from 6-10 week old Sprague-Dawley rats were cut into 1x3 mm blocks whose long dimension was oriented either perpendicular or parallel to the long axis of the tibia. Some blocks were fixed at pH 7. 0 in 0. 2M cacodylate - buffered 2. 5% glutaraldehyde for 1 hour at 4ÅC. The blocks were immersed in 30% glycerol solution at 4ÅC for 1 hour, frozen in liquid nitrogen, and then fractured, etched for 2 minutes, and coated with platinum, carbon and 0. 2% Formvar solution. The replicas were cleaned with chromic acid, floated onto Formvar coated grids, and examined with a Phillips EM 300 electron microscope.Fixed and unfixed specimens appeared similar in ultrastructure. Chondrocytes, matrix, and matrix vesicles were identified. In specimens fractured parallel to the long axis of the tibia, the reserve, proliferative, hypertrophic, and calcifying zones could be discerned as described by light and electron microscopy.

Sarcolemmal permeability changes during early myocardial anoxia

1978 ◽  
Vol 36 (2) ◽  
pp. 642-643
Author(s):  
M. Ashraf ◽  
L. Landa ◽  
L. Nimmo ◽  
C. M. Bloor
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Cell Membrane Permeability ◽  
Enzyme Release ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Sarcolemmal Permeability ◽  
Membrane Changes

Following coronary artery occlusion, the myocardial cells lose intracellular enzymes that appear in the serum 3 hrs later. By this time the cells in the ischemic zone have already undergone irreversible changes, and the cell membrane permeability is variably altered in the ischemic cells. At certain stages or intervals the cell membrane changes, allowing release of cytoplasmic enzymes. To correlate the changes in cell membrane permeability with the enzyme release, we used colloidal lanthanum (La+++) as a histological permeability marker in the isolated perfused hearts. The hearts removed from sprague-Dawley rats were perfused with standard Krebs-Henseleit medium gassed with 95% O2 + 5% CO2. The hypoxic medium contained mannitol instead of dextrose and was bubbled with 95% N2 + 5% CO2. The final osmolarity of the medium was 295 M osmol, pH 7. 4.

Light and electron microscopic investigation of adenohypophyses of germfree, food-restricted, and germfree-food restricted aging, male lobund-wistar rats

1988 ◽  
Vol 46 ◽  
pp. 352-353
Author(s):  
G. Ilse ◽  
K. Kovacs ◽  
N. Ryan ◽  
T. Sano ◽  
L. Stefaneanu ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Microscopic Investigation ◽  
Electron Microscopic Investigation ◽  
Aging Male ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Sprague Dawley Rats ◽  
Old Rats ◽  
Ad Libitum ◽  
Microscopic Findings

Germfree state and food restriction have been shown to increase life span and delay tumor occurrence in rats. We report here the histologic, immunocytochemical and electron microscopic findings of adenohypophyses of aging, male Lobund-Wistar rats raised at Lobund Laboratories. In our previous study, the morphologic changes in the adenohypophyses of old rats have been extensively investigated by histology, immunocytochemistry and electron microscopy. Lactotroph adenomas were frequent in Long-Evans and Sprague-Dawley rats, whereas gonadotroph adenomas were frequent in Sprague-Dawley and Wistar rats.Male Lobund-Wistar rats were divided into four groups: 1) conventional, which were raised under normal non-germfree environment and received food ad libitum; 2) germfree-food ad libitum; 3) conventional environment-food restricted and 4) germfree-food restricted. The adenohypophyses were removed from 6-month-, 18-month- and 30-month-old rats. For light microscopy, adenohypophyses were fixed in formalin and embedded in paraffin.

Permeability of the microcirculation in area postrema of rat

1988 ◽  
Vol 46 ◽  
pp. 348-349
Author(s):  
Shams M. Ghoneim ◽  
Frank M. Faraci ◽  
Gary L. Baumbach
Keyword(s):  
Brain Stem ◽  
Area Postrema ◽  
Upper Body ◽  
Sprague Dawley ◽  
Sodium Cacodylate ◽  
Acute Hypertension ◽  
Sprague Dawley Rats ◽  
Ultrastructural Characteristics ◽  
The Brain ◽  
Adjacent Brain

The area postrema is a circumventricular organ in the brain stem and is one of the regions in the brain that lacks a fully functional blood-brain barrier. Recently, we found that disruption of the microcirculation during acute hypertension is greater in area postrema than in the adjacent brain stem. In contrast, hyperosmolar disruption of the microcirculation is greater in brain stem. The objective of this study was to compare ultrastructural characteristics of the microcirculation in area postrema and adjacent brain stem.We studied 5 Sprague-Dawley rats. Horseradish peroxidase was injected intravenously and allowed to circulate for 1, 5 or 15 minutes. Following perfusion of the upper body with 2.25% glutaraldehyde in 0.1 M sodium cacodylate, the brain stem was removed, embedded in agar, and chopped into 50-70 μm sections with a TC-Sorvall tissue chopper. Sections of brain stem were incubated for 1 hour in a solution of 3,3' diaminobenzidine tetrahydrochloride (0.05%) in 0.05M Tris buffer with 1% H2O2.

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