Highly efficient in vitro translation of authentic affinity-purified messenger ribonucleoprotein complexes

RNA ◽

10.1261/rna.065730.118 ◽

2018 ◽

Vol 24 (7) ◽

pp. 982-989 ◽

Author(s):

Sarah E. Fritz ◽

Nazmul Haque ◽

J. Robert Hogg

Keyword(s):

In Vitro Translation ◽

Highly Efficient ◽

Ribonucleoprotein Complexes ◽

Messenger Ribonucleoprotein

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In vitro translation of mRNAs that are in their native ribonucleoprotein complexes

Biochemical Journal ◽

10.1042/bj20150772 ◽

2015 ◽

Vol 472 (1) ◽

pp. 111-119 ◽

Author(s):

Baptiste Panthu ◽

Fabrice Mure ◽

Henri Gruffat ◽

Theophile Ohlmann

Keyword(s):

Binding Proteins ◽

In Vitro Translation ◽

Ribosome Binding ◽

Ribonucleoprotein Complexes ◽

The present study shows the impact of ribosome binding proteins on in vitro translation.

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Highly efficient ribosome display selection by use of purified components for in vitro translation

Journal of Immunological Methods ◽

10.1016/j.jim.2006.04.001 ◽

2006 ◽

Vol 313 (1-2) ◽

pp. 140-148 ◽

Author(s):

Denis Villemagne ◽

Ronald Jackson ◽

Julie A. Douthwaite

Keyword(s):

In Vitro Translation ◽

Ribosome Display ◽

Highly Efficient

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An assessment of the masked message hypothesis: Sea urchin egg messenger ribonucleoprotein complexes are efficient templates for in vitro protein synthesis

Developmental Biology ◽

10.1016/0012-1606(82)90126-9 ◽

1982 ◽

Vol 93 (2) ◽

pp. 389-403 ◽

Author(s):

Randall T. Moon ◽

Michael V. Danilchik ◽

Merrill B. Hille

Keyword(s):

Protein Synthesis ◽

In Vitro Protein Synthesis ◽

Ribonucleoprotein Complexes ◽

Sea Urchin Egg ◽

Messenger Ribonucleoprotein ◽

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A Highly Efficient and Robust In Vitro Translation System for Expression of Picornavirus and Hepatitis C Virus RNA Genomes

Methods in Enzymology - Translation Initiation: Extract Systems and Molecular Genetics ◽

10.1016/s0076-6879(07)29004-4 ◽

2007 ◽

pp. 53-82 ◽

Author(s):

Yuri V. Svitkin ◽

Nahum Sonenberg

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

In Vitro Translation ◽

Translation System ◽

Hepatitis C Virus Rna ◽

Highly Efficient ◽

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Effects of estrogens on in vitro translation and on in vitro transcription in isolated rat liver nuclei

Acta Endocrinologica ◽

10.1530/acta.0.120s179 ◽

1989 ◽

Vol 120 (3_Suppl) ◽

pp. S179

Author(s):

C. BANDEL-SCHLESSELMANN ◽

E. R. LAX

Keyword(s):

Rat Liver ◽

In Vitro Transcription ◽

In Vitro Translation ◽

Isolated Rat Liver ◽

Rat Liver Nuclei ◽

Liver Nuclei ◽

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Heterogeneity of messenger RNA that encodes the rat insulin receptor is limited to the domain of exon 11. Analysis by RNA heteroduplex mapping, amplification of cDNA, and in vitro translation

Diabetes ◽

10.2337/diabetes.41.10.1293 ◽

1992 ◽

Vol 41 (10) ◽

pp. 1293-1300 ◽

Author(s):

B. J. Goldstein ◽

A. L. Dudley

Keyword(s):

Insulin Receptor ◽

Messenger Rna ◽

In Vitro Translation ◽

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Faculty Opinions recommendation of Potyvirus genome-linked protein, VPg, directly affects wheat germ in vitro translation: interactions with translation initiation factors eIF4F and eIFiso4F.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1097775.553895 ◽

2008 ◽

Author(s):

Andy Maule

Keyword(s):

Translation Initiation ◽

In Vitro Translation ◽

Initiation Factors ◽

Translation Initiation Factors

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Faculty Opinions recommendation of An in vitro translation, selection and amplification system for peptide nucleic acids.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1745956.2061082 ◽

2010 ◽

Author(s):

Dehua Pei ◽

Tao Liu

Keyword(s):

Nucleic Acids ◽

Peptide Nucleic Acids ◽

In Vitro Translation ◽

Amplification System

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Faculty Opinions recommendation of An in vitro translation, selection and amplification system for peptide nucleic acids.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1745956.1742054 ◽

2010 ◽

Author(s):

Sherry Mowbray ◽

Mikael Nilsson

Keyword(s):

Nucleic Acids ◽

Peptide Nucleic Acids ◽

In Vitro Translation ◽

Amplification System

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DEVELOPMENT OF APPROACHES FOR DETECTION OF GENOME-INTEGRATED PROVIRAL DNA OF THE HUMAN IMMUNODEFICIENCY VIRUS (HIV-1) IN ULTRA LOW CONCENTRATIONS USING THE CRISPR/CAS SYSTEM

Molecular Diagnostics and Biosafety – 2020. Russian national scientific and practical conference with international participation (October, 6–8, 2020): Conference Proceedings ◽

10.36233/978-5-9900432-9-9-118 ◽

2020 ◽

Author(s):

M.A. Tyumentseva ◽

◽

A.I. Tyumentsev ◽

V.G. Akimkin ◽

◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

Health Monitoring ◽

Biological Samples ◽

Proviral Dna ◽

Ribonucleoprotein Complexes ◽

Low Concentrations ◽

Immunodeficiency Virus ◽

For the effective functioning of supervisory and health monitoring services, it is necessary to introduce modern molecular technologies into their practice. Therefore, the task of developing new effective methods for detecting pathogen, for example HIV, based on CRISPR/CAS genome editing systems, remains urgent. In the present work, guide RNAs and specific oligonucleotides were developed for preliminary amplification of highly conserved regions of the HIV-1 genome. The developed guide RNAs make it possible to detect single copies of HIV-1 proviral DNA in vitro as part of CRISPR/CAS ribonucleoprotein complexes in biological samples after preliminary amplification.

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