Isolation of novel bacteria and actinomycetes using soil-extract agar medium

2005 ◽  
Vol 99 (5) ◽  
pp. 485-492 ◽  
Author(s):  
Takefumi Hamaki ◽  
Motomasa Suzuki ◽  
Ryosuke Fudou ◽  
Yasuko Jojima ◽  
Takayuki Kajiura ◽  
...  
Keyword(s):  
Agar Medium ◽  
Soil Extract ◽  
Extract Agar ◽  
Novel Bacteria ◽  
Soil Extract Agar

PLATE COUNTS OF BACTERIA AND FUNGI IN A SALINE SOIL

1959 ◽  
Vol 5 (5) ◽  
pp. 431-439 ◽  
Author(s):  
Norman James
Keyword(s):  
Saline Soil ◽  
Potassium Phosphate ◽  
Soil Extract ◽  
Red River ◽  
Replica Plating ◽  
Extract Agar ◽  
Plate Counts ◽  
Agar Media ◽  
Bacteria And Fungi ◽  
Soil Extract Agar

Numbers of bacteria and of fungi in a saline soil were about one-fifth of numbers in a Red River clay soil. Bacterial counts on two soil-extract agar media, one prepared from the saline soil and the other from Red River soil, were the same, and the populations on the two were the same as shown by the replica plating technique. They were larger than counts on sodium albuminate agar or on asparagin–mannitol agar. Likewise, fungal counts on either soil-extract agar, with 0.02% dipotassmm phosphate, 0.1% glucose, and 0.1% peptone, were higher than counts on Waksman's acidified medium, or on Martin's medium, or on Smith and Dawson's medium. Interestingly, fungal counts on a medium with the same three chemicals as the soil-extract media but with the soil extract replaced by water were as high as those on the soil-extract media. Different levels of potassium phosphate were tested in each of the above media. In each medium for bacteria, and in each for fungi, counts varied inversely as the amount of potassium phosphate. The same held true when sodium phosphate was used instead of potassium phosphate in each medium.

EFFECT OF PLATING MEDIUM AND INCUBATION TEMPERATURE ON GROWTH OF FUNGI IN SOIL-DILUTION PLATES

1963 ◽  
Vol 9 (6) ◽  
pp. 741-751 ◽  
Author(s):  
Donald D. Kaufman ◽  
Lansing E. Williams ◽  
Charles B. Sumner
Keyword(s):  
Sugar Beet ◽  
Incubation Temperature ◽  
Soil Extract ◽  
Plate Method ◽  
Dilution Plate ◽  
Extract Agar ◽  
Dilution Plate Method ◽  
Incubation Temperatures ◽  
Soil Dilution ◽  
Soil Extract Agar

The effects of 20, 25, or 30 °C incubation temperatures for dilution-plates and of five plating media (Ohio Agricultural Experiment Station Medium (OAES), Liftman"s crystal-violet agar, dextrose-peptone agar, soil-extract agar, and glucose-nitrate soil-extract agar) on total numbers and types of fungi isolated from two soils (sugar beet or corn cropped) by a soil-dilution plate method were determined. The data revealed that significant differences existed among the three variables as well as their interactions. Although numbers of colonies of fungi isolated were not affected significantly, the types of fungi (number of individual genera) isolated were significantly greater in sugar beet than in corn cropped soils. In nearly all instances, the greatest total number and types of fungi were isolated at temperatures of 20 or 25 °C. Although each medium appeared to favor one or more groups of fungi, the overall frequency and distribution of fungi was essentially the same on all media. On the basis of its transparency, total number and types of fungi isolated, the elimination of bacteria and actinomycetes, and its restriction of rapidly growing fungi, the OAES medium was deemed the most suitable for use in the soil-dilution plate method.

Accuracy and precision of population estimates of Verticillium dahliae on growth media in quantitative soil assays

2003 ◽  
Vol 81 (4) ◽  
pp. 294-306 ◽  
Author(s):  
S I Mpofu ◽  
R Hall
Keyword(s):  
Population Density ◽  
Verticillium Dahliae ◽  
Soil Extract ◽  
Population Estimates ◽  
Dilution Method ◽  
Growth Media ◽  
Infested Soil ◽  
Extract Agar ◽  
Accuracy And Precision ◽  
Soil Extract Agar

Verticillium dahliae Kleb. is a serious pathogen of many plant species. Growth media used to measure population density of V. dahliae in soil were evaluated for high recovery of the pathogen, as well as accuracy and precision of population estimates from naturally and artificially infested sandy loam soil using the soil dilution method. Recovery of V. dahliae from naturally infested field soil was highest on soil pectate Tergitol agar (SPT), soil extract agar + sodium polypectate (SEAP), modified pectate agar (MPA), potato dextrose agar + streptomycin sulphate (PDAS), and Talboys' prune lactose agar (TPA); however, PDAS and TPA were overgrown with contaminating fungi, making enumeration difficult. Use of SPT medium resulted in the most precise population estimate with a standard error (SE) of 12.6% of the mean followed by use of pectate agar (PA) (SE = 14.8%) and SEAP (SE = 19.1%). Ethanol, biotin, and Dox salts enhanced recovery of V. dahliae from naturally infested soil, but combining Dox salts with ethanol and biotin significantly reduced population density. Soil extract had no significant effects on population density. Accuracy of V. dahliae population estimates from sterile artificially inoculated soil was highest on modified soil extract agar (MSEA) (64%) followed by SPT (58%). However, accuracy of V. dahliae population estimates from nonsterile artificially inoculated soil was highest on SPT (36%). Soil extract is not an essential ingredient and biotin may increase recovery of V. dahliae from soil.Key words: Verticillium dahliae, verticillium wilt, population density, recovery, accuracy, precision.

scholarly journals Increased culturability of soil bacteria from Marcellus shale temperate forest in Pennsylvania

2013 ◽  
Vol 2 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Evan Mitchell Lutton ◽  
Rosa Schellevis ◽  
Anupama Shanmuganathan
Keyword(s):  
16S Rdna ◽  
Soil Bacteria ◽  
Temperate Forest ◽  
Culture Medium ◽  
Culture Media ◽  
Marcellus Shale ◽  
Soil Extract ◽  
Extract Agar ◽  
Unique Otus ◽  
Soil Extract Agar

Soil bacteria comprise a largely untapped resource with only 1-10% of bacterial species predicted to live in soil being culturable in the laboratory. Establishing culture-dependent protocols that identify unique operational taxonomic units (OTUs) is an important research topic in soil bacterial ecology. The culturability of soil bacteria may be improved by employing different culture media due to inherent preferences of growth substrate utilization. Soil-extract agar, R-2A agar, and 1% nutrient agar were used in this study to isolate bacteria obtained from soil samples collected in winter months to increase the understanding of bacterial diversity in Abernathy Field Station, a Marcellus shale temperate forest in Washington, Pennsylvania. Changes in bacterial diversity can be used to assess the early impact of anthropological factors, such as hydraulic fracturing in the Marcellus shale region, which may lead to severe environmental problems. For the purpose of long term ecological monitoring, data obtained from this year’s sample collection were analyzed in conjunction with previous years’ assessments. Bacterial isolates were analyzed taxonomically and phylogenetically. Unique OTUs were identified through comparative analysis of 16S rDNA. The Shannon-Weaver and Simpson’s diversity indices ranked isolates on soil-extract agar highest for species richness, and rarefaction analysis suggests that sampling saturation of OTUs identified on soil-extract agar has not yet been reached. Each culture medium studied supported isolates of four common phyla: Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. Soil-extract agar supported the greatest proportion of pigmented colonies including a Cyanobacterium which exhibited intra-16S rDNA polymorphism. Each culture medium supported the growth of unique OTUs and genera with Bacillus, Flavobacterium, Pseudomonas, Rhizobium, and Streptomyces found on each. This study suggests that utilizing different culture media can increase the culturability of soil bacteria.

scholarly journals Caracterização de isolados de Rhizoctonia associados à queima foliar em Roraima.

2012 ◽  
Vol 6 (2) ◽  
pp. 158 ◽  
Author(s):  
Dayane Rodrigues Youssef ◽  
Giovanni Ribeiro de Souza ◽  
Kátia De Lima Nechet ◽  
Bernardo De Almeida Halfeld-Vieira
Keyword(s):  
Glycine Max ◽  
Rhizoctonia Solani ◽  
Vigna Unguiculata ◽  
Lactuca Sativa ◽  
Hevea Brasiliensis ◽  
Cajanus Cajan ◽  
Citrullus Lanatus ◽  
Soil Extract ◽  
Extract Agar ◽  
Soil Extract Agar

O objetivo desse trabalho foi caracterizar isolados do fungo Rhizoctonia associados à queima foliar, obtidos de hospedeiros de importância econômica no estado de Roraima. Os isolados foram obtidos de plantas de feijão-caupi (Vigna unguiculata), soja (Glycine max), seringueira (Hevea brasiliensis), melancia (Citrullus lanatus), alface (Lactuca sativa) e feijão-guandu (Cajanus cajan). Os parâmetros utilizados foram números de núcleos, grupo de anastomose e as características culturais da colônia, taxa de crescimento micelial e a formação de escleródios nos meios de cultura: batata dextrose agar (BDA), BDA+asparagina, BDA+extrato de levedura, Czapek Agar, maltose-peptona-agar, soil extract agar, sacarose-yeast-asparagina e V-8. Todos os 10 isolados estudados foram caracterizados como multinucleados e pertencentes à espécie Rhizoctonia solani. Três isolados de feijão-caupi, um de soja e o isolado de melancia foram identificados como AGI-1A e um isolado de feijãocaupi, um de soja e o isolado de feijão-guandu como AGI-1B. O isolado de seringueira não foi identificado como nenhum dos padrões de anastomose utilizado. Para a maioria dos isolados as maiores taxas de crescimento micelial foram obtidas no meio de cultura Soil Extract Agar. Dois tipos de escleródios, característicos do grupo AGI, foram observados: formação de 2-20 tufos.placa-1 coloração variável, 1-2 mm e formação de 38-611 microescleródios placa-1, de coloração marrom, medindo 100 μm. A produção e o tipo de escleródio variaram com o isolado e o meio de cultura utilizado.

SOIL-EXTRACT AGAR FOR CANDIDA ALBICANS

1954 ◽  
Vol 69 (6) ◽  
pp. 735 ◽  
Author(s):  
ALEXANDER BAKERSPIGEL
Keyword(s):  
Candida Albicans ◽  
Soil Extract ◽  
Extract Agar ◽  
Soil Extract Agar

Fruiting of Lyophyllum tylicolor in plate culture on Soytoneglucose agar and urea-treated soil extract agar

Mycoscience ◽  
1994 ◽  
Vol 35 (2) ◽  
pp. 187-189 ◽  
Author(s):  
Takashi Yamanaka
Keyword(s):  
Soil Extract ◽  
Treated Soil ◽  
Extract Agar ◽  
Soil Extract Agar

A Comparison of the effects of several concentrations of oxgall in platings of soil fungi

1951 ◽  
Vol 29 (1) ◽  
pp. 26-31 ◽  
Author(s):  
J.J. Miller ◽  
D.J Peers ◽  
R.W. Neal
Keyword(s):  
Soil Fungi ◽  
Soil Extract ◽  
Potato Dextrose Agar ◽  
Crowding Effect ◽  
Extract Agar ◽  
Bacteria And Fungi ◽  
Soil Extract Agar

A study was made of the effect of adding varying concentrations of dehydrated bile to soil extract agar and potato dextrose agar in platings from two Ontario soils. The development of colonies of bacteria and actinomycetes was retarded, but no decrease was noted in the number of fungus colonies that appeared in the plates. The crowding effect of certain bacteria and fungi was lessened, facilitating the counting and isolation of individual fungus cultures.

EFFECT OF NUTRITION ON GROWTH AND CHLAMYDOSPORE FORMATION IN BROWN AND GRAY CULTURES OF THIELAVIOPSIS BASICOLA

1956 ◽  
Vol 34 (4) ◽  
pp. 459-472 ◽  
Author(s):  
R. H. Stover
Keyword(s):  
Agar Medium ◽  
Soil Extract ◽  
Methyl Green ◽  
Thielaviopsis Basicola ◽  
Wild Type ◽  
Ecological Studies ◽  
Chlamydospore Formation ◽  
Thiamine Content ◽  
Agar Media ◽  
Soil Extract Agar

Physiological differences between brown and gray wild type cultures of Thielaviopsis basicola were sought in order to develop selective media for use in mutational studies, and as a basis for pathogenicity and ecological studies. The gray wild type was more thiamine-deficient, yielded less mycelium, and grew more slowly than the brown on all media tested regardless of composition and thiamine content. In liquid media both types were more thiamine-deficient on sucrose than on glucose, but the reverse was true on agar media. The gray type was inhibited more than the brown by malachite and methyl green. A two-layered thiamine-deficient agar medium, and one containing dyes, was selective for the brown type. Gray and brown types grew better on thiamine-deficient water agar and soil extract agar than on thiamine-deficient agar media containing 20 gm/l. of sugar and 1 gm./l. of nitrogen compounds. On thiamine-deficient media, distortion and extrusion of the contents of endoconidia and hyphae were more common with the gray wild type than the brown.Endoconidia, but not chlamydospores, were formed in varying amounts on all thiamine-deficient media. The addition of thiamine resulted in the abundant production of chlamydospores and greatly increased growth. Chlamydospore production was also influenced by the source of nitrogen. Certain amino acids were more inhibitory to chlamydospore formation by the brown than gray type and vice versa. Asparagine, for example, blocked chlamydospore formation by the brown but not the gray type, even though growth and endoconidial production were abundant. Mutants capable of overcoming this blockage were readily produced in culture.

THE INFLUENCE OF SOIL AND ROOT EXTRACTS ON THE ASSOCIATIVE GROWTH OF SELECTED SOIL BACTERIA

1963 ◽  
Vol 9 (2) ◽  
pp. 187-197 ◽  
Author(s):  
E. C. S. Chan ◽  
H. Katznelson ◽  
J. W. Rouatt
Keyword(s):  
Mixed Culture ◽  
Soil Bacteria ◽  
Agar Medium ◽  
Soil Extract ◽  
Antagonistic Effect ◽  
Pseudomonas Sp ◽  
Population Changes ◽  
Agrobacterium Radiobacter ◽  
Root Extracts ◽  
Wheat Population

These studies are concerned with the growth interrelationships of mixed cultures of five soil organisms in soil extract and root extracts of 2-, 4-, and 8-week-old oats, soybeans, and wheat. Population changes of Agrobacterium radiobacter, Arthrobacter citreus, Azotobacter chroococcum, Bacillus cereus, and a Pseudomonas sp. in pure and mixed culture were followed by plating on selective media. B. cereus and A. chroococcum grew poorly alone or in mixed culture in the extracts. In soil extract, A. citreus predominated over, or was nearly equal in number to, the Gram-negative forms (Pseudomonas and Agrobacterium). In root extracts, Pseudomonas sp. always predominated over A. citreus in mixed culture. A. radiobacter was inhibited in mature root extracts (8-week-old plants) although in pure culture it recovered after a period. An antagonistic effect of Pseudomonas sp. on A. chroococcum plated on nitrogen-free agar medium was found to be related to the kind of agar used.

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