scholarly journals Genome analysis of Agrobacterium tumefaciens: Linkage map and genetic features of the left region of the linear chromosome

2001 ◽  
Vol 76 (6) ◽  
pp. 363-371 ◽  
Author(s):  
Devika M. De Costa ◽  
Katsunori Suzuki ◽  
Megumi Satou ◽  
Kazuo Yoshida
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Linkage Map ◽  
Genome Analysis ◽  
Linear Chromosome ◽  
Genetic Features ◽  
Left Region

scholarly journals Draft Genome Sequence of Agrobacterium tumefaciens Strain 1D1526

2019 ◽  
Vol 8 (45) ◽  
Author(s):  
Naxin Huo ◽  
Yong Gu ◽  
Kent F. McCue ◽  
Diaa Alabed ◽  
James G. Thomson
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Circular Chromosome ◽  
Linear Chromosome ◽  
Content Type

This work reports the draft genome sequence of Agrobacterium tumefaciens strain 1D1526. The assembled genome is composed of a 2,881,823-bp circular chromosome, a 2,235,711-bp linear chromosome, and a 44,582-bp unassembled contig.

scholarly journals Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini

BMC Genomics ◽  
2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Claire Anderson ◽  
Muhammad Adil Khan ◽  
Ann-Maree Catanzariti ◽  
Cameron A. Jack ◽  
Adnane Nemri ◽  
...  
Keyword(s):  
Gene Cloning ◽  
Linkage Map ◽  
Genome Analysis ◽  
Rust Fungus ◽  
High Density ◽  
Avirulence Gene ◽  
Melampsora Lini

RFLP linkage map and genome analysis of Saccharum spontaneum

Genome ◽  
1993 ◽  
Vol 36 (4) ◽  
pp. 782-791 ◽  
Author(s):  
Jorge A. G. da Silva ◽  
Mark E. Sorrells ◽  
William L Burnquist ◽  
Steven D. Tanksley
Keyword(s):  
Linkage Map ◽  
Genome Size ◽  
Genome Analysis ◽  
Dna Probes ◽  
Rflp Markers ◽  
Saccharum Spontaneum ◽  
Linkage Groups ◽  
Rflp Map ◽  
Dna Libraries ◽  
Rice Cdna

An RFLP linkage map of the wild sugarcane species Saccharum spontaneum L. (2n = 8x = 40–128) was constructed, comprising 216 loci, detected by 116 DNA probes, and distributed over 44 linkage groups. At a density of at least one marker every 25-cM interval, the coverage of the genome was estimated as 86%. For the generation of RFLP markers, probes were surveyed from seven DNA libraries: three sugarcane cDNA, one oat cDNA, one rice cDNA, and one barley cDNA, as well as one sugarcane genomic. Sixty-two maize genomic clones that were previously mapped on maize were used to initiate a comparative map between the sugarcane, sorghum, and maize genomes. Based on the RFLP segregation data, we conclude that this species is an autopolyploid, with an estimated genome size of 2107 cM.Key words: sugarcane, polyploid, RFLP, map, genome.

scholarly journals Reconciliation of Sequence Data and Updated Annotation of the Genome of Agrobacterium tumefaciens C58, and Distribution of a Linear Chromosome in the Genus Agrobacterium

2012 ◽  
Vol 79 (4) ◽  
pp. 1414-1417 ◽  
Author(s):  
Steven Slater ◽  
João C. Setubal ◽  
Brad Goodner ◽  
Kathryn Houmiel ◽  
Jian Sun ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Evolutionary History ◽  
Sequence Data ◽  
Additional Analysis ◽  
Linear Chromosome ◽  
Content Type ◽  
History Of ◽  
Agrobacterium Tumefaciens C58

ABSTRACTTwo groups independently sequenced theAgrobacterium tumefaciensC58 genome in 2001. We report here consolidation of these sequences, updated annotation, and additional analysis of the evolutionary history of the linear chromosome, which is apparently limited to the biovar I group ofAgrobacterium.

scholarly journals Replicon-Specific Regulation of Small Heat Shock Genes in Agrobacterium tumefaciens

2004 ◽  
Vol 186 (20) ◽  
pp. 6824-6829 ◽  
Author(s):  
Sylvia Balsiger ◽  
Curdin Ragaz ◽  
Christian Baron ◽  
Franz Narberhaus
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Heat Shock ◽  
Elevated Temperatures ◽  
Heat Shock Gene ◽  
Circular Chromosome ◽  
Linear Chromosome ◽  
Shsp Gene ◽  
Shine Dalgarno Sequence ◽  
Specific Regulation ◽  
Shock Proteins

ABSTRACT Four genes coding for small heat shock proteins (sHsps) were identified in the genome sequence of Agrobacterium tumefaciens, one on the circular chromosome (hspC), one on the linear chromosome (hspL), and two on the pAT plasmid (hspAT1 and hspAT2). Induction of sHsps at elevated temperatures was revealed by immunoblot analyses. Primer extension experiments and translational lacZ fusions demonstrated that expression of the pAT-derived genes and hspL is controlled by temperature in a regulon-specific manner. While the sHsp gene on the linear chromosome turned out to be regulated by RpoH (σ32), both copies on pAT were under the control of highly conserved ROSE (named for repression of heat shock gene expression) sequences in their 5′ untranslated region. Secondary structure predictions of the corresponding mRNA strongly suggest that it represses translation at low temperatures by masking the Shine-Dalgarno sequence. The hspC gene was barely expressed (if at all) and not temperature responsive.

scholarly journals Combined Genetic and Physical Map of the Complex Genome of Agrobacterium tumefaciens

1999 ◽  
Vol 181 (17) ◽  
pp. 5160-5166 ◽  
Author(s):  
Brad W. Goodner ◽  
Brian P. Markelz ◽  
M. Casey Flanagan ◽  
Chris B. Crowell ◽  
Jodi L. Racette ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Physical Mapping ◽  
Physical Map ◽  
Genomic Structure ◽  
Genetic Maps ◽  
Mapping Data ◽  
Circular Chromosome ◽  
Linear Chromosome ◽  
Single Chromosome ◽  
Complex Genome

ABSTRACT A combined genetic and physical map of the Agrobacterium tumefaciens A348 (derivative of C58) genome was constructed to address the discrepancy between initial single-chromosome genetic maps and more recent physical mapping data supporting the presence of two nonhomologous chromosomes. The combined map confirms the two-chromosome genomic structure and the correspondence of the initial genetic maps to the circular chromosome. The linear chromosome is almost devoid of auxotrophic markers, which probably explains why it was missed by genetic mapping studies.

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