scholarly journals Laboratory-based dried blood spot testing for hepatitis C: A protocol for systematic review and meta-analysis of diagnostic accuracy

2020 ◽  
Vol 3 ◽  
pp. 78
Author(s):  
Paul G. Carty ◽  
Michael McCarthy ◽  
Sinead O’Neill ◽  
Patricia Harrington ◽  
Michelle O’Neill ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Meta Analysis ◽  
Venous Blood ◽  
Hcv Infection ◽  
Likelihood Ratios ◽  
Blood Samples ◽  
Reflex Testing ◽  
Chronic Hcv Infection ◽  
Chronic Hcv ◽  
Blood Spot

Background: Diagnosis of chronic hepatitis C virus (HCV) infection typically involves collection of venous blood samples prior to serological investigation of an antibody response followed by a confirmatory viral load or antigen test to verify active HCV infection. This conventional pathway poses logistical challenges for the implementation of reflex testing, whereby the confirmatory test is performed on the same sample used for serological investigation. Dried blood spot (DBS) testing, in which capillary blood is deposited on filter paper, is a less invasive alternative that can enable reflex testing without the need for venepuncture, centrifugation and freezing of samples. Methods: This systematic review aims to assess the diagnostic accuracy of DBS compared with venous blood samples for diagnosis of chronic HCV infection. Observational studies which compare diagnostic tests using DBS with those using serum, plasma or whole blood in patients with chronic or resolved HCV infection will be included. Electronic searches will be conducted in PubMed, Embase, Scopus, Web of Science, Lilacs and the Cochrane library. Citation screening, data extraction and quality appraisal of included studies will be performed in duplicate using the Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) tool. A meta-analysis will be conducted to derive pooled estimates of sensitivity, specificity, positive likelihood ratios, negative likelihood ratios, and diagnostic odds ratios. Sensitivity analyses and meta-regression will also be performed. Quality of the evidence will be evaluated using the GRADE criteria. Discussion: Identifying and linking people with currently undiagnosed chronic HCV infection to care is pivotal to attaining global viral hepatitis elimination targets. The use of DBS could simplify diagnostic testing strategies by integrating reflex testing into the care pathway and reducing drop-off along the cascade of care. Registration: PROSPERO, CRD42020205204. Registered 19th September 2020.

Association of chronic hepatitis C virus infection with an increased risk of lung cancer: A systematic review and meta-analysis.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e13562-e13562
Author(s):  
Ben Ponvilawan ◽  
Nipith Charoenngam ◽  
Pongprueth Rujirachun ◽  
Phuuwadith Wattanachayakul ◽  
Surapa Tornsatitkul ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Systematic Review ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Search Strategy ◽  
Meta Analysis ◽  
Hcv Infection ◽  
Increased Risk ◽  
Chronic Hcv Infection ◽  
Chronic Hcv

e13562 Background: Chronic hepatitis C virus (HCV) infection is associated with increased risk of multiple types of extrahepatic cancer, such as lymphomas, thyroid cancer and renal cancer. However, whether HCV infection also increases the risk of lung cancer is still inconclusive. This systematic review and meta-analysis was performed in order to determine the relationship between chronic HCV infection and lung cancer. Methods: A systematic review was performed using EMBASE and MEDLINE databases from inception to November 2019 with search strategy that represents “hepatitis C virus” and “cancer”. Eligible studies must be cohort studies which include patients with chronic HCV infection and comparators without HCV infection, then follow them for incident lung cancer. Relative risk, incidence rate ratio (IRR), standardized incidence ratio, or hazard risk ratio of this association along with associated 95% confidence interval (CI) from each study were extracted and combined for the calculation of the pooled effect estimate using the random effect, generic inverse variance. Results: 20,459 articles were discovered using the aforementioned search strategy. After two rounds of review, eight studies fulfilled the inclusion criteria and were included into the meta-analysis. Chronic HCV infection was significantly associated with increased risk of lung cancer with the pooled relative risk of 1.94 (95% CI, 1.56 – 2.42; I2 = 87%). Funnel plot was fairly symmetric and not suggestive of presence of publication bias. Conclusions: Chronic HCV infection is significantly associated with a 1.94-fold increased risk in the development of lung cancer compared to no infection.

scholarly journals Performance of HCV Antigen Testing for the Diagnosis and Monitoring of Antiviral Treatment: A Systematic Review and Meta-Analysis

2022 ◽  
Vol 2022 ◽  
pp. 1-17
Author(s):  
Geane Lopes Flores ◽  
Jurema Corrêa Mota ◽  
Larissa Tropiano da Silva Andrade ◽  
Renata Serrano Lopes ◽  
Francisco Inácio Bastos ◽  
...  
Keyword(s):  
Systematic Review ◽  
Diagnostic Accuracy ◽  
Meta Analysis ◽  
Antiviral Treatment ◽  
Hcv Infection ◽  
Hcv Rna ◽  
Eligibility Criteria ◽  
Cross Sectional ◽  
High Concordance ◽  
Chronic Hcv

Background and Aims. Active hepatitis C virus (HCV) infection is based on the detection of HCV RNA that it is effective but presents high cost and the need to hire trained personnel. This systematic review and meta-analysis is aimed at evaluating the diagnostic accuracy of HCV Ag testing to identify HCV cases and to monitor antiviral treatment including DAA treatment. Methods. The studies were identified through a search in PubMed, Lilacs, and Scopus from 1990 through March 31, 2020. Cohort, cross-sectional, and randomized controlled trials were included. Two independent reviewers extracted data and assessed quality using an adapted Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool. Our primary outcome was to determine the accuracy of HCV Ag detection for the diagnosis, which we estimated using random-effects meta-analysis. Results. Of 3,062 articles identified, 54 met our eligibility criteria. The studies described cohorts from 20 countries, including 14,286 individuals with chronic HCV individuals. Studies for ECLIA technology demonstrated highest quality compared to studies that used ELISA. The pooled sensitivity and specificity (95% CI) for HCV Ag detection of active HCV infection were 98.82% ( 95 % CI = 98.04 %; 99.30%) and 98.95% ( 95 % CI = 97.84 %; 99.49%), respectively. High concordance was found between HCV Ag testing and HCV RNA detection 89.7% and 95% to evaluate antiviral treatment. Conclusions. According to our findings, HCV Ag testing could be useful to identify HCV active cases in low-resource areas. For antiviral treatment, HCV Ag testing will be useful at the end of treatment.

Thyroid Disturbance in Patients with Chronic Hepatitis C Infection: A Systematic Review and Meta-analysis

2016 ◽  
Vol 25 (2) ◽  
pp. 227-234 ◽  
Author(s):  
Yi Shen ◽  
Xu-Lin Wang ◽  
Jin-Ping Xie ◽  
Jian-Guo Shao ◽  
Yi-Hua Lu ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Meta Analysis ◽  
Random Effect ◽  
Hcv Infection ◽  
Interferon Α ◽  
Thyroid Peroxidase ◽  
Thyroid Antibodies ◽  
Thyroid Peroxidase Antibody ◽  
Chronic Hcv Infection ◽  
Chronic Hcv

Background & Aims: The involvement of thyroid autoimmunity and dysfunction in patients with chronichepatitis C virus (HCV) infection before interferon-α (IFN-α) therapy remains controversial. We performedthis meta-analysis to evaluate the association of HCV infection with the presence of anti-thyroid antibodiesand dysthyroidism. Methods: A literature search was carried out to collect articles dated up to August 2015 to identify observationalstudies which compared the prevalence of anti-thyroid antibodies and thyroid dysfunction in IFN-α naïvechronic HCV-infected subjects with non-HCV infected controls. Random-effect or fixed-effect meta-analyseswere applied and results reported as odds ratios (ORs) with 95% confidence intervals (CIs). Results: Twelve studies were included, involving 1,735 HCV-infected and 1,868 non-HCV infected subjects.Pooled anti-thyroid antibody prevalence tended to be higher in HCV-infected subjects. The prevalence ofanti-thyroglobulin antibody (TGAb), anti-thyroid peroxidase antibody (TPOAb), anti-thyroid microsomalantibody (ATMA) were 2.40-fold, 1.96-fold and 1.86-fold higher in HCV-infected subjects than in controls,respectively. The prevalence of hypothyroidism also differed by HCV infection status, with a pooled risk of3.10 (95%CI: 2.19-4.40) in HCV-infected subjects. However, the results did not show a significant differencein the prevalence of hyperthyroidism between the two groups.Conclusion: Chronic HCV infection may be an independent risk factor for thyroid disturbance. It is advisablefor the clinicians to monitor both thyroid antibodies and function in the course of chronic HCV infection,independent of IFN-α treatment. Abbreviations: AITD: autoimmune thyroid disease; ATMA: anti-thyroid microsomal antibody; CHC:chronic hepatitis; GD: Graves’ disease; HCV: hepatitis C virus; HT: Hashimoto’s thyroiditis; IFN-α: interferonalpha;NOS: Newcastle-Ottawa quality assessment scale; T3: triiodothyronine; T4: thyroxine; TGAb: antithyroglobulinantibody; TPOAb: anti-thyroid peroxidase antibody.

scholarly journals Real-world effectiveness and safety of glecaprevir/pibrentasvir for the treatment of patients with chronic HCV infection: A meta-analysis

2020 ◽  
Vol 72 (6) ◽  
pp. 1112-1121 ◽  
Author(s):  
Pietro Lampertico ◽  
Jose A. Carrión ◽  
Michael Curry ◽  
Juan Turnes ◽  
Markus Cornberg ◽  
...  
Keyword(s):  
Real World ◽  
Meta Analysis ◽  
Hcv Infection ◽  
Chronic Hcv Infection ◽  
Chronic Hcv

Treatment of acute hepatitis C infection with interferon-alfa 2b monotherapy prevents development of chronic HCV infection

2001 ◽  
Vol 120 (5) ◽  
pp. A567-A567 ◽  
Author(s):  
E JAECKEL ◽  
M CORNBERG ◽  
T SANTANTONIO ◽  
J MAYER ◽  
H WEDEMEYER ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Acute Hepatitis ◽  
Interferon Alfa ◽  
Hcv Infection ◽  
Acute Hepatitis C ◽  
Hepatitis C Infection ◽  
Chronic Hcv Infection ◽  
Chronic Hcv

Diagnostic accuracy of magnetic resonance angiography for acute pulmonary embolism - a systematic review and meta-analysis

VASA ◽  
2016 ◽  
Vol 45 (2) ◽  
pp. 149-154 ◽  
Author(s):  
Jie Li ◽  
Lei Feng ◽  
Jiangbo Li ◽  
Jian Tang
Keyword(s):  
Pulmonary Embolism ◽  
Magnetic Resonance ◽  
Magnetic Resonance Angiography ◽  
Diagnostic Accuracy ◽  
Acute Pulmonary Embolism ◽  
Meta Analysis ◽  
Likelihood Ratios ◽  
Summary Receiver Operating Characteristic ◽  
Acute Pe ◽  
Limited Sensitivity

Abstract. Background: The aim of this meta-analysis was to evaluate the diagnostic accuracy of magnetic resonance angiography (MRA) for acute pulmonary embolism (PE). Methods: A systematic literature search was conducted that included studies from January 2000 to August 2015 using the electronic databases PubMed, Embase and Springer link. The summary receiver operating characteristic (SROC) curve, sensitivity, specificity, positive likelihood ratios (PLR), negative likelihood ratios (NLR), and diagnostic odds ratio (DOR) as well as the 95 % confidence intervals (CIs) were calculated to evaluate the diagnostic accuracy of MRA for acute PE. Meta-disc software version 1.4 was used to analyze the data. Results: Five studies were included in this meta-analysis. The pooled sensitivity (86 %, 95 % CI: 81 % to 90 %) and specificity (99 %, 95 % CI: 98 % to 100 %) demonstrated that MRA diagnosis had limited sensitivity and high specificity in the detection of acute PE. The pooled estimate of PLR (41.64, 95 % CI: 17.97 to 96.48) and NLR (0.17, 95 % CI: 0.11 to 0.27) provided evidence for the low missed diagnosis and misdiagnosis rates of MRA for acute PE. The high diagnostic accuracy of MRA for acute PE was demonstrated by the overall DOR (456.51, 95 % CI: 178.38 - 1168.31) and SROC curves (AUC = 0.9902 ± 0.0061). Conclusions: MRA can be used for the diagnosis of acute PE. However, due to limited sensitivity, MRA cannot be used as a stand-alone test to exclude acute PE.

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