Radiation-induced ICAM-1 Expression via TGF-β1 Pathway on Human Umbilical Vein Endothelial Cells; Comparison between X-ray and Carbon-ion Beam Irradiation

Osteosarcoma (OSA) is the most common malignant bone tumor in children and adolescents. The overall five-year survival rate for all bone cancers is below 70%; however, when the cancer has spread beyond the bone, it is about 15–30%. Herein, we evaluated the effects of carbon-ion beam irradiation alone or in combination with zoledronic acid (ZOL) on OSA cells. Carbon-ion beam irradiation in combination with ZOL significantly inhibited OSA cell proliferation by arresting cell cycle progression and initiating KHOS and U2OS cell apoptosis, compared to treatments with carbon-ion beam irradiation, X-ray irradiation, and ZOL alone. Moreover, we observed that this combination greatly inhibited OSA cell motility and invasion, accompanied by the suppression of the Pi3K/Akt and MAPK signaling pathways, which are related to cell proliferation and survival, compared to individual treatments with carbon-ion beam or X-ray irradiation, or ZOL. Furthermore, ZOL treatment upregulated microRNA (miR)-29b expression; the combination with a miR-29b mimic further decreased OSA cell viability via activation of the caspase 3 pathway. Thus, ZOL-mediated enhancement of carbon-ion beam radiosensitivity may occur via miR-29b upregulation; co-treatment with the miR-29b mimic further decreased OSA cell survival. These findings suggest that the carbon-ion beam irradiation in combination with ZOL has high potential to increase OSA cell death.

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Carbon-Ion Beam Irradiation Kills X-Ray-Resistant p53-Null Cancer Cells by Inducing Mitotic Catastrophe

PLoS ONE ◽

10.1371/journal.pone.0115121 ◽

2014 ◽

Vol 9 (12) ◽

pp. e115121 ◽

Cited By ~ 27

Author(s):

Napapat Amornwichet ◽

Takahiro Oike ◽

Atsushi Shibata ◽

Hideaki Ogiwara ◽

Naoto Tsuchiya ◽

...

Keyword(s):

Cancer Cells ◽

Ion Beam ◽

Mitotic Catastrophe ◽

Beam Irradiation ◽

Ion Beam Irradiation ◽

Carbon Ion ◽

X Ray ◽

Carbon Ion Beam

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Effect of captopril on radiation-induced TGF-β1 secretion in EA.Hy926 human umbilical vein endothelial cells

Oncotarget ◽

10.18632/oncotarget.15356 ◽

2017 ◽

Vol 8 (13) ◽

pp. 20842-20850 ◽

Cited By ~ 6

Author(s):

Jingni Wei ◽

Hui Xu ◽

Yinyin Liu ◽

Baiyu Li ◽

Fuxiang Zhou

Keyword(s):

Endothelial Cells ◽

Umbilical Vein ◽

Human Umbilical Vein ◽

Radiation Induced ◽

Umbilical Vein Endothelial Cells ◽

Tgf Β1

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p53- dependent hyperthermic enhancement of tumour growth inhibition by X-ray or carbon-ion beam irradiation

International Journal of Hyperthermia ◽

10.1080/02656730210166131 ◽

2003 ◽

Vol 19 (2) ◽

pp. 145-153 ◽

Cited By ~ 12

Author(s):

A. Takahashi ◽

I. Ota ◽

T. Tamamoto ◽

I. Asakawa ◽

Y. Nagata ◽

...

Keyword(s):

Growth Inhibition ◽

Tumour Growth ◽

Ion Beam ◽

Beam Irradiation ◽

Ion Beam Irradiation ◽

Carbon Ion ◽

X Ray ◽

Tumour Growth Inhibition ◽

Carbon Ion Beam

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Platelet-Derived Exosomes Affect the Proliferation and Migration of Human Umbilical Vein Endothelial Cells Via miR-126

Current Vascular Pharmacology ◽

10.2174/1570161116666180313142139 ◽

2019 ◽

Vol 17 (4) ◽

pp. 379-387 ◽

Cited By ~ 11

Author(s):

Yan Sun ◽

Xiao-li Liu ◽

Dai Zhang ◽

Fang Liu ◽

Yu-jing Cheng ◽

...

Keyword(s):

Endothelial Cells ◽

Growth Factor ◽

Real Time ◽

Umbilical Vein ◽

Angiogenic Factors ◽

Healthy Donors ◽

And Migration ◽

Umbilical Vein Endothelial Cells ◽

Tgf Β1 ◽

Proliferation And Migration

Background:Intraplaque angiogenesis, the process of generating new blood vessels mediated by endothelial cells, contributes to plaque growth, intraplaque hemorrhage, and thromboembolic events. Platelet-derived Exosomes (PLT-EXOs) affect angiogenesis in multiple ways. The ability of miR-126, one of the best-characterized miRNAs that regulates angiogenesis, carried by PLT-EXOs to influence angiogenesis via the regulation of the proliferation and migration of endothelial cells is unknown. In this study, we aimed to investigate the effects of PLT-EXOs on angiogenesis by Human Umbilical Vein Endothelial Cells (HUVECs).Methods:We evaluated the levels of miR-126 and angiogenic factors in PLT-EXOs from Acute Coronary Syndrome (ACS) patients and healthy donors by real-time Polymerase Chain Reaction (PCR) and western blotting. We incubated HUVECs with PLT-EXOs and measured cell proliferation and migration with the Cell Counting Kit-8 assay and scratch assay, respectively. We also investigated the expression of miR-126 and angiogenic factors in HUVECs after exposure to PLT-EXOs by western blotting and real-time PCR.Results:PLT-EXOs from ACS patients contained higher levels of miR-126 and angiogenic factors, including Vascular Endothelial Growth Factor (VEGF), basic Fibroblast Growth Factor (bFGF), and Transforming Growth Factor Beta 1 (TGF-β1), than those from healthy donors (p<0.05). Moreover, the levels of exosomal miR-126 and angiogenic factors were increased after stimulation with thrombin (p<0.01). HUVEC proliferation and migration were promoted by treatment with activated PLT-EXOs (p<0.01); they were accompanied by the over-expression of miR-126 and angiogenic factors, including VEGF, bFGF, and TGF-β1 (p<0.01).Conclusion:Activated PLT-EXOs promoted the proliferation and migration of HUVECs, and the overexpression of miR-126 and angiogenic factors, thereby elucidating potential new therapeutic targets for intraplaque angiogenesis.

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