scholarly journals Formation and metabolism of pungent principles of Capsicum fruits. II. In vivo and in vitro formation of dihydrocapsaicin in sweet pepper fruits, Capsicum annuum L. var. grossum.

1977 ◽  
Vol 41 (10) ◽  
pp. 1877-1882 ◽  
Author(s):  
Kazuo IWAI ◽  
Tetsuya SUZUKI ◽  
Kap-Rang LEE ◽  
Masahiro KOBASHI ◽  
Sinzaburo OKA
Keyword(s):  
Capsicum Annuum ◽  
Sweet Pepper ◽  
Capsicum Annuum L

scholarly journals NADPH Oxidase (Rboh) Activity is Up Regulated during Sweet Pepper (Capsicum annuum L.) Fruit Ripening

Antioxidants ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 9 ◽  
Author(s):  
Ángela Chu-Puga ◽  
Salvador González-Gordo ◽  
Marta Rodríguez-Ruiz ◽  
José M. Palma ◽  
Francisco J. Corpas
Keyword(s):  
Oxidative Stress ◽  
Nadph Oxidase ◽  
Capsicum Annuum ◽  
Fruit Ripening ◽  
Biochemical Characterization ◽  
Sweet Pepper ◽  
Oxidative Stress Marker ◽  
Vitro Assay ◽  
Capsicum Annuum L

In plants, NADPH oxidase (NOX) is also known as a respiratory burst oxidase homolog (Rboh). This highly important enzyme, one of the main enzymatic sources of superoxide radicals (O2•−), is involved in the metabolism of reactive oxygen and nitrogen species (ROS and RNS), which is active in the non-climacteric pepper (Capsicum annuum L.) fruit. We used sweet pepper fruits at two ripening stages (green and red) to biochemically analyze the O2•−-generating Rboh activity and the number of isozymes during this physiological process. Malondialdehyde (MDA) content, an oxidative stress marker, was also assayed as an index of lipid peroxidation. In red fruits, MDA was observed to increase 2-fold accompanied by a 5.3-fold increase in total Rboh activity. Using in-gel assays of Rboh activity, we identified a total of seven CaRboh isozymes (I–VII) which were differentially modulated during ripening. CaRboh-III and CaRboh-I were the most prominent isozymes in green and red fruits, respectively. An in vitro assay showed that CaRboh activity is inhibited in the presence of nitric oxide (NO) donors, peroxynitrite (ONOO−) and glutathione (GSH), suggesting that CaRboh can undergo S-nitrosation, Tyr-nitration, and glutathionylation, respectively. In summary, this study provides a basic biochemical characterization of CaRboh activity in pepper fruits and indicates that this O2•−-generating Rboh is involved in nitro-oxidative stress associated with sweet pepper fruit ripening.

scholarly journals Exploring the Nutraceutical Potential of Dried Pepper Capsicum annuum L. on Market from Altino in Abruzzo Region

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 400 ◽  
Author(s):  
Alice Della Valle ◽  
Marilisa Pia Dimmito ◽  
Gokhan Zengin ◽  
Stefano Pieretti ◽  
Adriano Mollica ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Sweet Pepper ◽  
Biological Properties ◽  
Hot Pepper ◽  
Local Product ◽  
Abruzzo Region ◽  
Typical Type ◽  
Anti Inflammatory

Sweet pepper is a typical type of Capsicum annuum from Abruzzo region, recognized as a traditional and local product, traditionally cultivated in the town of Altino (Abruzzo region, Italy). The aim of this study is to compare the sweet type of peppers from Altino with the hot pepper cultivated in the same area, in order to delineate their different phytochemical and biological profiles in vitro and in vivo. In this study, we elucidated their phytochemical composition, fatty acids composition and phenolic/flavonoid contents in extracts. Then antioxidant and enzyme inhibition assays were performed to evaluate their biological properties, together with in vitro cell assay and in vivo anti-inflammatory activity. Microwave (1000 mg/mL) extract of hot pepper showed the best inhibition value on in vitro cell growth assay; in fact, the number of survived cells was about 20% and 40% for microwave and Soxhlet extracts, respectively. In vivo anti-inflammatory assay revealed good activity for both species, which, when associated with in vitro cell inhibition results, could explain the protective effect on human prostatic hyperplasia.

scholarly journals Cytotoxic, antioxidant and antimicrobial properties of red sweet pepper (Capsicum annuum L. var. Llanerón) extracts: In vitro study

2017 ◽  
Vol 6 (2) ◽  
Author(s):  
Rosa Raybaudi-Massilia ◽  
Alírica I. Suárez ◽  
Francisco Arvelo ◽  
Alexandra Zambrano ◽  
Felipe Sojo ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Sweet Pepper ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Capsicum Annuum L ◽  
Vitro Effect ◽  
Mcf 7

Alcoholic and aqueous extracts were obtained from red sweet pepper (Capsicum annuum L.) by different methodologies to evaluate their cytotoxic, antioxidant and antimicrobial properties. Alcoholic extracts (MFP, MSd, SFP, SDP, SSd) from fresh red sweet pepper (FP) and dry pulp (DP) and seed (Sd) were obtained by maceration (M) and Soxhlet (S) equipment using methanol as extraction solvent; whereas aqueous extracts (LFP, LSd) were obtained by decoction followed by lyophilization (L). Human tumoral cell lines from breast (MCF-7 and SKBr3), prostate (PC3) and cervix (HeLa), and fibroblasts (as control) were used to determine the cytotoxic properties by the MTT assay. Antioxidant and antimicrobial properties were determined by DPPH and disc diffusion method, respectively. The extracts SDP and SFP showed the higher cytotoxic activity. The SDP extract had a significant (P < 0.05) in-vitro effect on HeLa (1.9 ± 1.4 µg/mL) and PC3 (< 1 µg/mL) cells with a moderated impact on fibroblasts (26.1 ± 1.2 µg/mL); whereas, SFP had a significant (p < 0.05) effect on MCF-7 cell line (2.1 ± 1.2 µg/mL) with a moderated impact on fibroblasts (25.9 ± 1.0 µg/mL). The higher antioxidant activity was found for MFP (80.3 ± 0.2%) and SFP extracts (75.5 ± 0.5%). Mild antimicrobial activity was only observed for alcoholic extracts. The results showed the potential of red sweet pepper (C. annuum L.) as a source of antioxidant and cytotoxic compounds, and suggest the need of further studies to isolate and characterize the bioactive compounds that impart those properties.

scholarly journals Antineoplastic effect of pectic polysaccharides from green sweet pepper (Capsicum annuum) on mammary tumor cells in vivo and in vitro

2018 ◽  
Vol 201 ◽  
pp. 280-292 ◽  
Author(s):  
Eliana Rezende Adami ◽  
Claudia Rita Corso ◽  
Natalia Mulinari Turin-Oliveira ◽  
Claudia Martins Galindo ◽  
Letícia Milani ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Capsicum Annuum ◽  
Mammary Tumor ◽  
Sweet Pepper ◽  
Pectic Polysaccharides ◽  
Mammary Tumor Cells ◽  
Antineoplastic Effect

scholarly journals Anastomosis grouping of Rhizoctonia solani and binucleate Rhizoctonia spp. isolated from pepper in Erzincan, Turkey

2013 ◽  
Vol 49 (No. 3) ◽  
pp. 127-131 ◽  
Author(s):  
S. Tuncer ◽  
C. Eken
Keyword(s):  
Rhizoctonia Solani ◽  
Capsicum Annuum ◽  
Binucleate Rhizoctonia ◽  
Anastomosis Groups ◽  
Capsicum Annuum L

Ninety eight isolates of Rhizoctonia spp. were obtained from roots of pepper (Capsicum annuum L.) grown in Erzincan, Turkey during the period 2007&ndash;2008. The most prevalent multinucleate anastomosis groups (AG) were AG-4 (85.2%), followed by AG-2 type 1 (7.4%), AG-6 (5.0%), and AG-3 (2.5%). The population of binucleate Rhizoctonia spp. comprised AG-A (82.4%), AG-K (11.8%), and AG-G (5.9%). Rhizoctonia solani AG-3 and AG-6, as well as binucleate Rhizoctonia spp. AG-G and AG-K on pepper (C. annuum) were firstly determined in this study. During both in vitro and in vivo pathogenicity experiments differences in virulence level between R. solani and binucleate Rhizoctonia spp. isolates were observed. Isolates of R. solani AG-2 type 1 and AG-4 were the most virulent, binucleate Rhizoctonia spp. isolates of AG-A were less virulent, whereas binucleate Rhizoctonia spp. isolates of AG-G and AG-K were non-pathogenic. &nbsp;

scholarly journals The Amendment with Chitin and/or Chitosan Improves the Germination and Growth of Lycopersicon esculentum L., Capsicum annuum L. and Solanum melongena L.

2020 ◽  
Author(s):  
Rkhaila Amine ◽  
El Hartiti Abla ◽  
Bouziani Idrissi Mohammed ◽  
Ounine Khadija
Keyword(s):  
Lycopersicon Esculentum ◽  
Capsicum Annuum ◽  
Solanum Melongena ◽  
Germination Percentage ◽  
In Vivo Test ◽  
Capsicum Annuum L ◽  
Chitin And Chitosan ◽  
Germination And Growth

Background: Chitin and chitosan are natural products extracted from shrimp shells. They are non-toxic, biocompatible and biodegradable which favors many applications in agricultural domain. Moreover, chitin and chitosan are known as stimulators of photosynthetic rates and enhance pants growth through minerals contains (C, O, N ….). In the present study, these co-products are evaluated for their ability to stimulate the germination and growth of Lycopersicon esculentum L., Capsicum annuum L. and Solanum melongena L. Methods: The experiments were conducted at Ibn Tofail University-Kenitra, between July 2016 to January 2018. The experiments are carried out by using a germination test for the three seeds. On the other hand, the in vivo test is used to determine the effect of the biopolymers on plant growth (length, fresh and dry weights of aerial and root parts, number and weight of the obtained fruits). Result: In vitro studies reveal a promoter action of chitosan and the mixture chitin-chitosan on increasing seed germination percentage by 16%, 34% and 22%. Thus, at both 25, 50 and 100 mg/l of chitin or chitosan, increase vigor index, shoot length and root is obtained. In vivo, the effect of chitin and chitosan is limited to stimulation of the lengths, fresh and dry weights of plants. However, a highly significant increase in the number and weight of the fruits is marked by the weekly soil amendment by the chitin-chitosan mixture at 25 mg/l and 100 mg/l.

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