scholarly journals Inhibitory effects of leaf extracts from Fagaceae on mutation in a streptomycin-dependent mutant of Salmonella typhimurium TA 98 under assay conditions without S-9 mix.

1989 ◽  
Vol 53 (10) ◽  
pp. 2807-2808 ◽  
Author(s):  
Akiyoshi HOSONO ◽  
Toshihiro KISHI ◽  
Hajime OTANI
Keyword(s):  
Salmonella Typhimurium ◽  
Inhibitory Effects ◽  
Leaf Extracts ◽  
Assay Conditions

scholarly journals Correction: Inhibitory Effects of Hydroethanolic Leaf Extracts of Kalanchoe brasiliensis and Kalanchoe pinnata (Crassulaceae) against Local Effects Induced by Bothrops jararaca Snake Venom

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0172598 ◽  
Author(s):  
Júlia Morais Fernandes ◽  
Juliana Félix-Silva ◽  
Lorena Medeiros da Cunha ◽  
Jacyra Antunes dos Santos Gomes ◽  
Emerson Michell da Silva Siqueira ◽  
...  
Keyword(s):  
Snake Venom ◽  
Inhibitory Effects ◽  
Leaf Extracts ◽  
Local Effects ◽  
Bothrops Jararaca ◽  
Kalanchoe Pinnata

scholarly journals Allelopathic effect of Azadirachta indica L. on the germination of Abelmoschus esculentus L.

2014 ◽  
Vol 15 ◽  
pp. 13-22 ◽  
Author(s):  
T. Vaithiyanathan ◽  
M. Soundari ◽  
M. Rajesh ◽  
K. Sankar Ganesh ◽  
P. Sundaramoorthy
Keyword(s):  
Azadirachta Indica ◽  
Photosynthetic Pigment ◽  
Research Work ◽  
Chemical Compounds ◽  
Abelmoschus Esculentus ◽  
Allelopathic Effect ◽  
Root Extract ◽  
Root Bark ◽  
Inhibitory Effects ◽  
Leaf Extracts

The chemical compounds have been reported to be exuded by plants and their inhibitory effects of other plants. The research work was carried out to study the allelopathic effect of root, bark and leaf extracts of Azadirachta indica L. on the seed germination of Abelmoschus esculentus L. Germination studies were conducted in laboratory, the bhendi seeds treated with the root, bark and leaf extracts of Neem. On the seventh day the morphological parameters, photosynthetic pigment and biochemical contents were analysed. The result showed that the rate of germination of bhendi decreased in all Azadirachta indica L. extracts. The reduction in biochemical contents of bhendi was also noted. The highest rate of germination was observed in control (distilled water) treatment and the highest rate of inhibition was observed in root extract followed by bark and leaf extracts.

scholarly journals Changes in the Ripening Characteristics and Shelf Life of Mango Fruits as Related to the Application of Coating Based on Cocoa Leaf Extracts

2019 ◽  
pp. 1-13
Author(s):  
Kouete Jarvin Ovaric ◽  
Phounzong-Tafre Eugène ◽  
Aghofack-Nguemezi Jean
Keyword(s):  
Shelf Life ◽  
Nutritional Value ◽  
Soluble Solids ◽  
Edible Coatings ◽  
Soluble Solids Content ◽  
Inhibitory Effects ◽  
Leaf Extracts ◽  
Ripening Process ◽  
Solids Content ◽  
Β Carotene

The influence of edible coatings based on cocoa leaf extracts on the ripening of ''Belle-Dame'' mangoes has been evaluated, to contribute to the improvement of the shelf life of these fruits after harvest. There were overall treatment-independent decreases in the firmness and chlorophylls levels, and increases in total soluble solids content, water content, physiological weight loss and β-carotene concentration during the ripening of mango fruits. These variations were very rapid in control fruits, reflecting an accelerated ripening process that led to the senescence of these fruits from day 9 after harvest onwards. In treated fruits, variations of different parameters were slow with significant differences between the treated fruits and controls. These inhibitory effects of coatings resulted in a delay of the ripening process and consequently to a shift of the onset of senescence to 12 days after harvest. In addition to the extension of shelf life by three days, treatments of mango fruits by dipping them in solutions containing cocoa leaf extracts induced a higher accumulation of β-carotene in coated fruits as compared to controls, thereby enhancing the nutritional value of the former.

scholarly journals Investigating Anti-mutagenic Activities of Lantana camara L. (Verbenaceae) Applying Salmonella typhimurium and the Ames Test

2020 ◽  
Vol 5 (3) ◽  
pp. 90-95
Author(s):  
Zahra Zare
Keyword(s):  
Salmonella Typhimurium ◽  
Ames Test ◽  
Petri Dish ◽  
Botanical Garden ◽  
Positive Control ◽  
Test Methods ◽  
Leaf Extracts ◽  
Percolation Method ◽  
Methanolic Extracts ◽  
The Mean

Introduction: Genetic mutations have a significant role in causing cancers, and plants are effective on cancer recovery by producing metabolites. In this regard, the present study aimed to evaluate the Lantana camera anti-mutation effects applying Salmonella typhimurium in the Ames test. Methods: To this end, the plant was prepared from the Iran National Botanical Garden in 2018 (Tehran, Iran), and the methanolic extracts of its leaves and flowers were obtained by the percolation method. Then, anti-mutagenic activities were studied by the Ames method and the assessment of the rate of reverse mutations in mutant Salmonella typhimurium. Mutant strains cannot grow on minimal mineral media thus only those bacteria that have acquired a wild genotype after reverse mutation in the presence of the mutagen are able to grow on this medium. The plant extract, along with a mutagen substance was used to evaluate its anti-mutagenic effects by counting grown colonies and calculating the mean mutation inhibitory index according to the "Ong" formula. Finally, anti-mutagenic activities were retested by adding the sterile extract of the mouse liver (S9), and the data were analyzed by SPSS statistical software, version 22.Results: In general, the results showed that the mean number of grown colonies decreased significantly despite the plant material in comparison with the standard. According to the "Ong" formula, the percentage of inhibition was [1-T/M]×100. Based on the results, T grew a number of colonies on each petri dish despite the mutagen and extract, and M grew a number of colonies in positive control plates. Eventually, mutation inhibition percentages in leaf extracts were significantly higher than those of flower extracts, which were 75.59 ± 0.73 (+S9) and 84.79 ± 0.17 (-S9), as well as 49.57 ± 0.55 (+S9) and 62.32 ± 0.23 (-S9), respectively (P < 0.05). Conclusion: In general, the leaves and flowers of L. camara demonstrated anti-mutagenic activities with higher activities in the leaves compared to flowers.

Evaluation of the effects of Rumex obtusifolius seed and leaf extracts against Acanthamoeba: An in vitro study

2020 ◽  
Vol 20 ◽  
Author(s):  
Tooran Nayeri ◽  
Farahnaz Bineshian ◽  
Fariba Khoshzaban ◽  
Abdolhossein Dalimi Asl ◽  
Fatemeh Ghaffarifar
Keyword(s):  
Flow Cytometry ◽  
Culture Medium ◽  
In Vitro Study ◽  
Vital Staining ◽  
Seed Extract ◽  
Clinical Strain ◽  
Inhibitory Effects ◽  
Leaf Extracts ◽  
Rumex Obtusifolius

Background: Acanthamoebiasis treatment is a major and challenging problem due to the presence of resistant cyst form. Many herbal extracts and their derivatives have been used against trophozoites and cysts of Acanthamoeba, but no effective therapeutic agent has yet been discovered. Therefore, the present study aimed to evaluate the effect of Rumex obtusifolius (R. obtusifolius) extracts against a clinical strain of Acanthamoeba genotype T4 in vitro. Methods: In this experimental study, after genotyping the clinical isolate, the hydroalcohlic extracts of R. obtusifolius seeds and leaves were prepared. Different concentrations (1.25, 2.5, 5 and 10 mg/ml) of extracts were tested in triplicate (24, 48 and 72h) on trophozoites and cysts of Acanthamoeba. Mortality of the parasite was assessed by trypan blue vital staining and flow cytometry analysis. Results: Results showed that the extract of R. obtusifolius leaves at the concentration of 10 mg/ml killed 100% of trophozoites and cysts after 72 h. However, the seed extract of R. obtusifolius had weak inhibitory effects on trophozoites and cysts of Acanthamoeba. In the presence of 10 mg/ml of hydroalcohlic seed extract of R.obtusifolius in culture medium after 72 h, 28.6% of trophozoites and 0% of cysts of Acanthamoeba were killed. After analysis by flow cytometry, seeds and leaves extracts indicated apoptosis effect. Seed and leaf of extracts caused 2.6% and 0.4% percent apoptosis. Conclusion: These extracts are not promising candidates for further medicine development on acanthamoebiasis. Nonetheless, further researches are necessary to clarify effective fractions of seed and leaf extracts of R. obtusifolius and their mechanisms of action.

scholarly journals Inhibitory Effects of Collagen on the PCR for Detection of Clostridium perfringens

2000 ◽  
Vol 66 (3) ◽  
pp. 1213-1215 ◽  
Author(s):  
Sangburm Kim ◽  
Ronald G. Labbe ◽  
Sangryeol Ryu
Keyword(s):  
Clostridium Perfringens ◽  
Rapid Detection ◽  
Extraction Methods ◽  
Magnesium Ion ◽  
Inhibitory Effects ◽  
Food Components ◽  
Pcr Method ◽  
Dna Extraction Methods ◽  
Assay Conditions ◽  
Specific Food

ABSTRACT It is essential to identify specific food components that inhibit PCR in order to increase the sensitivity of the PCR method for rapid detection of pathogens contaminating a food. We found that collagen, a major component of several foods, inhibited PCR. The inhibitory action of collagen on PCR could be partially reversed by adjusting the concentration of magnesium ion in the reaction mixture and by the use of various DNA extraction methods to remove the collagen from the DNA. Also, the source of thermostable DNA polymerase was affected by the presence of collagen. These results suggest the need to optimize the extraction and assay conditions for rapid detection of enterotoxigenicClostridium perfringens by PCR with respect to the kind of food being analyzed.

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